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Using disk electrophoresis in the polyacrylamide gel, blood serum proteins were studied in miners working under conditions of the combine (the control group) and drilling-and-blasting (the contact with carbon oxide, nitrogen oxides) driving technique under normal temperature conditions. 26--27 protein fractions characterized by mobility, thermolability under definite conditions of the experiment and the contitative content were obtained. It is shown that the contact with carbon oxide and nitrogen oxides causes changes in the rpoperties of certain proteins (II3, globulins--2 alpha 1, 3 alpha 1, 2 beta, 2 alpha 2, 5 alpha 2, 6 alpha 2, 7 alpha 2) of miners blood serum. Some of these proteins are supposed to participate in the adaptation reactions of the organism.

A successive treatment with sodium iodide and digitonin of the myeline, microsome and synaptosome fractions of the cattle brain tissue resulted in obtaining the preparations of "soluble", Na+, K+-ATPase with a rather high specific activity non-containing practically Mg2+-dependent ATPase. To estimate the degree of these preparations structural heterogeneity they were subjected to analytical ultracentrifugation and disc-electrophoresis in polyacrylamide gel. The data obtained suggest an insignificant degree of heterogeneity for the "soluble" Na+, K+-ATP-ase preparations.

Heterogeneity of proteins of blood serum high-molecular fraction in healthy people, patients with cancer and those with pathologies of nonmalignant nature was studied by the method of disc electrophoresis in 4% polyacrylamide gel. It is established that under conditions of the experiment sharp differences are observed in heterogeneity of studied proteins manifesting in a growth of the discrete electrophoretic bands in disc electrophoregrams of high-molecular fractions of sera from the cancer patients. Disc electrophoregrams of proteins of the studied fractions from the sera of patients with nonmalignant pathologies also differ from those of control groups, though not so sharply as when comparing the preparations obtained from the sera of healthy people and cancer patients. Possible reasons of the observed differences are discussed.