1. [Mechanisms of contractile reaction changes and efficiency of oxygen utilization by portal vein smooth muscles in experimental diabetes mellitus].
- Author
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Prysiazhna OD, Kotsiuruba AV, Tkachenko MM, and Sahach VF
- Subjects
- Animals, Diabetes Mellitus, Experimental metabolism, Hydrocarbons, Brominated, Isoenzymes metabolism, Male, Muscle, Smooth, Vascular physiology, Nitric Oxide metabolism, Nitric Oxide Synthase Type III metabolism, Oxidative Stress physiology, Portal Vein physiology, Rats, Rats, Inbred WKY, Diabetes Mellitus, Experimental physiopathology, Fluorocarbons metabolism, Muscle Contraction physiology, Muscle, Smooth, Vascular metabolism, Portal Vein metabolism
- Abstract
On the rat portal vein isolated strips the essential reduction of contractive response, increase of stiffness of vascular smooth muscles at their stretching and increase of oxygen value of smooth muscles' work are revealed in conditions of experimental diabetes mellitus. The possible mechanism of such infringements is chronic hyperglycemia, which results in development of oxidative stress and reduction of NO synthesis by constitutive NOS. Indeed, the significant decrease of activity of constitutive NO-synthase and increase of the oxidative stress markers contents are shown at experimental diabetes mellitus. The similar infringements are marked at in vitro model of hyperglycemia, blockade of NO-synthase and in conditions of oxidative stress. Additional confirmation of a role of NO synthesis blockade and oxidative stress in development of such infringements at diabetes is fact that application of antioxidant melatonin and L-arginine, a precursor of NO to diabetic animals results in partial restoration of contractive reactions, reduction of stiffness and oxygen value of smooth muscles' work.
- Published
- 2006