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Background. The relevance of finding optimal methods to treat patients with a comorbid non-alcoholic steatohepatitis (NASH) that developed against the background of type 2 diabetes mellitus (T2DM) is due to the fact that these diseases have a number of common cause-and-effect mechanisms, and if diabetic kidney disease (DKD) develops, also mutual burden mechanisms. The purpose of the study was to find out the possible influence of a combination of metformin, rosuvastatin, essential phospholipids and quercetin on the clinical course of non-alcoholic steatohepatitis, diabetic kidney disease, type 2 diabetes mellitus, as well as on the state of the blood lipids, parameters of carbohydrate metabolism compensation, the degree of insulin resistance, which are factors for the progression of NASH and diabetic kidney disease. Materials and methods. Studies were conducted on the dynamics of treatment in 60 patients with NASH, T2DM and DKD stage I–III: 48 (80.0 %) of them had mild NASH, and 12 (20.0 %) had moderate NASH. A comorbid disease in 100 % of patients was moderate type 2 diabetes: 15 (25.0 %) people were diagnosed with diabetes in the stage of compensation, 45 (75.0 %) had subcompensated disease. Results. The positive effect of quercetin was noted by us in relation to the content of low-density lipoprotein cholesterol in the blood that was increased by 1.8 times (p < 0.05) before the treatment: a decrease after it was 1.7 times (p < 0.05) in group 2 and 1.3 times (p < 0.05) in group 1. Comprehensive therapy with the inclusion of quercetin contributed to a probable increase in anti-atherogenic high-density lipoprotein (by 1.3 times, p < 0.05) with the normalization of the indicator after the treatment, while traditional therapy in this contingent did not lead to any probable changes. Conclusions. The combination therapy for type 2 diabetes mellitus and NASH with the addition of quercetin contributed to the elimination of the main clinical and laboratory symptoms of NASH exacerbation, a probable reduction in the liver inflammation (a decrease in markers of cytolysis, mesenchymal inflammation), reversal of hepatic steatosis due to the optimization of cholesterol and triacylglycerols in the blood, a probable increase in high-density lipoproteins, normalization of glycemia, reduction of insulinemia, a decrease in the degree of insulin resistance. The effectiveness of treatment for DKD was also increased: the rate of proteinuria and the degree of hypercreatinemia decreased, and the glomerular filtration rate increased.

Non-alcoholic fatty liver disease (NAFLD) or due modern nomenclature – metabolic dysfunction-associated steatotic liver disease (MASLD) and atrial fibrillation are interconnected health problems that require reassessment of treatment strategies to address their common underlying causes. Lifestyle changes and pharmacotherapy are used to manage NAFLD, while treatment for atrial fibrillation focuses on anticoagulation and rhythm control. Aim. To evaluate the efficacy of combining ursodesoxycholic acid and quercetin with standard treatment to improve liver health and heart function in patients with non-alcoholic fatty liver disease and atrial fibrillation. Material and methods. A prospective, randomized controlled trial was conducted on patients with NAFLD and atrial fibrillation at two hospitals from January 2020 to December 2023. The study involved 127 patients who were divided into three groups: standard treatment, standard treatment + ursodesoxycholic acid (UDCA), and standard treatment + UDCA + quercetin. Results. The average age and sex distribution were similar among the three groups, suggesting that the randomization process successfully balanced the demographic characteristics. Paroxysmal atrial fibrillation was the most common form in all groups, followed by persistent atrial fibrillation and permanent atrial fibrillation, with no statistically significant differences between the groups. The body mass index was comparable across all three groups as well. The results showed a decrease in liver stiffness in all groups, with groups 2 and 3 showing the most significant improvements. Fibrosis stages also shifted after treatment, with group 3 showing a marked reduction in progressive fibrosis. Groups 2 and 3 also showed significant reductions in steatosis levels, with an increase in the proportion of patients without steatosis. Left atrial diameter decreased in all groups, with group 3 showing the most significant reduction. Left ventricular ejection fraction improved in all groups, with the most significant increase in group 3. Group 3 also showed improvements in diastolic filling and left ventricular filling pressure. Overall, the combined treatment regimen in group 3 appeared to have the most favourable effects on liver and cardiac health. Conclusions. The addition of UDCA and quercetin to standard treatment regimens for NAFLD and atrial fibrillation shows promising improvements in liver health and cardiac function.

In the context of the modern industrialisation of poultry farming and the growing global demand for quail products, fnding effective ways to improve bird productivity and health is extremely important. One of the most promising approaches is the use of nanoselenium bio-compounds with probiotics to improve the metabolic parameters of quail. Recent research has focused on the synthesis of selenium nanoparticles using probiotics as an environmentally friendly alternative to traditional methods of adding inorganic selenium to quail feed. The advantage of this approach is the production of a biocompatible and bioavailable form of selenium, which provides birds with the ability to effectively absorb and use selenium for various physiological processes. The effect of innovative feed additives, such as selenium nanoconjugates and probiotics, on various metabolic parameters in quail was investigated. These include the activity of antioxidant defence enzymes, indicators of carbonyl oxidative stress, protein carbonyl levels and protein metabolism. By adding selenium nanoconjugates and probiotics to quail feed, an improvement in antioxidant defence mechanisms was observed, leading to a reduction in oxidative stress and an improvement in the overall health of the birds. In addition, improved protein metabolism as a result of these supplements has been shown to have a positive impact on the productivity and quality of quail products. In summary, investing in high quality feed additives such as selenium nanoconjugates and probiotics is a strategic approach to improving the productivity and proftability of poultry production. By taking advantage of the benefts of nanotechnology and probiotics, farmers can optimise the health and productivity of their poultry flocks, meeting the growing demand for quail products on the national market.

The relevance of this study stems from the growing interest in the impact of acute desynchronosis (AD) on oxidative disorders and the insufficiently studied effect of flavonoids on these processes in kidney tissue. This study investigates the effect of quercetin on oxidative and nitrosative stress in rat kidney homogenate under AD and 3 lipopolysaccharide (LPS)-induced systemic inflammatory response (SIR). The animals were randomly divided into five groups: group 1 served as the control, group 2 underwent LPS-induced SIR modeling for 8 weeks, group 3 experienced AD reproduction by shifting the light-dark cycle 6 hours earlier for 7 days, group 4 underwent AD modeling during the last week of LPS-induced SIR, and rats in group 5 were administered a water-soluble form of quercetin (corvitin) intragastrically was administered at a dose of 200 mg/kg (20 mg/kg in terms of quercetin) daily during the AD modeling against LPS-induced SIR. It was found that the modeling of LPS-induced oxidative stress and AD significantly increased the rate of superoxide anion radical production in kidney homogenate by microsomal monooxygenases and NO synthase (NOS), mitochondrial respiratory chain, leukocyte NADPH oxidase, inducible NOS activity, and decreased the activity of the constitutive NOS isoform and its coupling index. Under AD modeling, the rate of superoxide anion radical production by leukocyte NADPH oxidase, total and inducible NOS activity was significantly lower compared with LPS-induced SIR. When modeling AD against the background of LPS-induced SIR, the production of superoxide anion radical by microsomes, mitochondria and leukocyte NADPH oxidase exceeded the respective results of group 2 by 15.9%, 16.0% and 38.5%, and group 3 by 24.4%, 17.5% and 74.6%. The inducible NOS activity was 22.9% higher compared to the value of group 2 and 93.4% higher in group 3, the concentration of peroxynitrites of alkali and alkaline earth metals was 35.5% higher than the respective parameters of group 2 and 57.9% higher than in group 3. Administration of quercetin during AD modeling and LPS-induced oxidative stress significantly reduced the rate of superoxide anion radical production by microsomal monooxygenases by 17.1%, mitochondrial respiratory chain by 31.2%, leukocyte NADPH oxidase by 19.4%, total and inducible NOS activity by 50.4% and 55.5%, respectively; the concentration of peroxynitrites of alkali and alkaline earth metals diminished by 41.4% compared to the value of group 4. It has been concluded that the administration of quercetin significantly reduces the signs of oxidative-nitrosative stress in the kidney homogenate of rats. [ABSTRACT FROM AUTHOR]

Background. Cardiovascular diseases like coronary artery disease (CAD) are a major global health issue, causing millions of deaths each year. Type 2 diabetes mellitus (T2DM) worsens outcomes for those with CAD. Common risk factors suggest potential synergistic treatment approaches, including the use of quercetin, a natural flavonoid. The purpose was to examine the clinical efficacy and safety of empagliflozin and quercetin in the treatment of coronary artery disease and type 2 diabetes mellitus comorbidity in the light of their impact on parameters of inflammation. Materials and methods. A total of 128 patients diagnosed with both stable CAD and T2DM were enrolled in the study between January and December 2023. Participants were categorized into three groups. Group 1 (controls): 40 patients receiving the standard treatment protocol for CAD and T2DM. Group 2: 46 patients received standard therapy in addition to empagliflozin (10 mg daily). Group 3: 42 patients received standard therapy in addition to empagliflozin (10 mg daily) and quercetin (80 mg three times a day). Results. The results showed that age and gender were similar between the groups, indicating a balanced distribution. The study then evaluated the effects of treatment on various parameters. The baseline body mass index (BMI), glucose, glycated hemoglobin (HbA1c), soluble suppression of tumorigenicity 2 (sST2), Toll-like receptor 2 (TLR2) and TLR4 levels were comparable across all groups. After treatment, significant reductions were observed in BMI, fasting glucose, HbA1c, sST2, and TLR2/TLR4 in all groups. The most significant improvements were seen in the group that received both empagliflozin and quercetin. The findings highlight the potential benefits of these interventions in patients with coronary heart disease and type 2 diabetes mellitus. Conclusions. The addition of empagliflozin and quercetin to standard therapy appears to offer significant benefits in managing BMI, glycemic control, and inflammatory markers in patients with CAD and T2DM. The combined treatment regimen has potential to address the complex interplay of metabolic and inflammatory processes in high-risk patients. The proposed combination also showed efficacy in reducing the myocardial stress, which is shown by a decrease in serum sST2.

Background. Non-alcoholic fatty liver disease and chronic kidney disease are public health concerns worldwide due to their increasing prevalence, adverse prognosis, and health care burden. The purpose of the study was to determine the probable effect of a combination of metformin, rosuvastatin, essential phospholipids and quercetin on the blood lipids, endothelial function, fibrinolysis system and platelet hemostasis, which are factors for the progression of nonalcoholic steatohepatitis. Materials and methods. Studies were performed on the dynamics of treatment in 60 patients with non-alcoholic fatty liver disease, type 2 diabetes mellitus and diabetic kidney disease (stage I–III). Depending on the prescribed treatment at random, the examined patients were divided into 2 groups. Twenty-eight persons of the first group received a low-calorie diet with dietary restrictions, essential phospholipids, metformin hydrochloride, rosuvastatin. Thirty-two patients from the second group received quercetin in addition to similar dietary recommendations, essential phospholipids, hypoglycemic and hypolipidemic therapy. The mean age of patients was 53.80 ± 3.52 years. The comparison group consisted of 30 healthy individuals of the corresponding age. Results. To evaluate the degree of endothelial-protective effect of quercetin on the background of the recommended protocol therapy, markers of endothelial dysfunction, fibrinolysis and platelet hemostasis were studied. NO content significantly reduced (1.7 times) in patients of group 2 before treatment, increased by 1.5 times (p

This article investigates the impact of water-soluble form of quercetin (corvitin) on the depolymerization of the organic matrix biopolymers and biomechanical properties of the rat mandible following its incomplete fracture during chronic alcohol intoxication. The study was conducted on the 14th day after inducing chronic alcohol intoxication by daily intragastric administration of a 40% ethanol solution in a daily dose of 12 ml/kg body weight; on that day the incomplete fracture of the mandible was modelled by drilling the bone to a depth of 1 mm using a carbide drill with a diameter of 1.5 mm at a speed of up to 1000 rpm. The administration of a water-soluble form of quercetin for 14 days during the posttraumatic period in a daily dose of 10 mg/kg (in recalculation for quercetin), following an incomplete mandibular fracture during chronic alcohol intoxication, significantly reduced the activity of acid phosphatase and its tartrate-resistant isoform by 35.3% and 23.1%, respectively, as well as led to a decrease in the concentration of free hydroxyproline, hexuronic and N-acetylneuraminic acids by 17.0%, 30.6%, and 43.3%, respectively, compared to the findings revealed in animals, which did not receive the drug. The Young's modulus exceeded the values of this group by 72.3%, the elastic limit by 53.2% the tensile strength by 59.6%, and the relative elongation to failure by 30.0%. We concluded that the administration of a water-soluble form of quercetin for 14 days following an incomplete mandibular fracture during chronic alcohol intoxication improves reparative processes in the injured area; it also restricts bone resorption and the depolymerization of connective (bone) tissue biopolymers such as collagen, proteoglycans, and sialoglycoproteins. Under these conditions, the biomechanical properties of the mandibular bone in the fracture area, particularly its elasticity and strength, are significantly enhanced. [ABSTRACT FROM AUTHOR]

The objective: to evaluate the effectiveness of the combined medication, which includes ascorbic acid (vitamin C), vitamin D3 (cholecalciferol), quercetin, and zinc oxide in the treatment and prevention of complications of acute respiratory viral infections (ARVI) at the outpatient level. Materials and methods. 5,982 patients (42.68% men and 57.32% women) aged 18 years and older with symptoms of ARVI participated in the study. The study was conducted in two stages: 1) diagnosis of ARVI and prescribing treatment, including a combined medication; 2) final assessment of the effectiveness, safety and tolerability of therapy. The total duration of participation in the study for one patient was no more than 1 month. In the complex treatment of ARVI at the outpatient level, according to the instructions, 1, 2 or 3 tablets per day were prescribed, depending on the severity of the course of the disease. The duration of treatment was from 5 to 30 days. 1 tablet of the combined medication includes ascorbic acid (vitamin C) - 100 mg, sodium ascorbate - 450 mg (equivalent to ascorbic acid - 400 mg), vitamin D3 (cholecalciferol) - 1000 IU, quercetin - 250 mg, zinc oxide (equivalent to elemental zinc) - 10 mg. Efficacy was evaluated based on the frequency of bacterial complications of ARVI, which required the antibiotic therapy. The safety of the therapy was assessed by the frequency of adverse outcomes and their severity. Results. The main indication for the use of the combined medication was ARVI (83% of patients) without etiological clarification, 5% of patients were diagnosed with COVID-19 and 11% - influenza. In 6% of patients the drug was prescribed for prophylactic purposes and for non-infectious diseases. Clinical manifestations of acute bronchitis were found in 12% of patients with ARVI, acute sinusitis - in 8% of patients. Bacterial complications (pneumonia, otitis, bacterial sinusitis, and bronchitis) in which there is a need to use systemic antibiotics occurred in 4.79% (95% CI: 4.27-5.35%) of ARVI patients which have received the combined medication. The frequency of prescribing systemic antibiotics increased in proportion to the severity of the disease and the age of the patients. Adverse non-serious, rare side effects when using the combined medication were found in 0.56% of patients who participated in the study. Conclusions. The combined medication, which includes ascorbic acid (vitamin C), vitamin D3 (cholecalciferol), quercetin, zinc oxide, has good tolerance and can be recommended to alleviate the condition of patients with ARVI, prevent complications, shorten the duration of treatment and promote faster recovery of the patient. [ABSTRACT FROM AUTHOR]

The study is aimed at investigating the impact of exogenous melatonin and quercetin on indices of systemic inflammatory response and indicators of carbohydrate and lipid metabolism in the blood serum of male rats exposed to round-the-clock lighting (RCL) with 1500 lx intensity during the last 30 days of being kept on a 60 day high-calorie carbohydrate-lipid diet (HCCLD, 20% fructose solution and the proper chow). The study has demonstrated that the restoration of serum melatonin concentration in rats by its exogenous administration during the RCL exposure and keeping them on HCCLD did not fully correct the indicators of the systemic inflammatory response such as the content of tumor necrosis factor α (TNF-α) and C-reactive protein (CRP), as well as indices of carbohydrate and lipid metabolism such as concentration of insulin, highdensity lipoprotein (HDL), very low-density lipoprotein (VLDL) and triglycerides, HOMA-IR insulin resistance index. The administration of quercetin under the experimental conditions was accompanied by a significant increase in the serum melatonin concentration (by 85.9%), a decrease in the content of TNF-α (by 53.9%), CRP (by 54.4%), glucose (by 49.2%), insulin (by 49.6%), VLDL (by 49, 2%) and triglycerides (by 49.3%), and an increase in HDL concentration (by twofold), but these indicators (with the exception of the HOMA-IR index, which decreased by 62.4%) did not reach the values of the intact group. The combined effect of melatonin and quercetin under RCL exposure and HLLD significantly improved the indicators of systemic inflammatory response, carbohydrate and lipid metabolism that is confirmed by a more significant decrease in serum levels of TNF-α, CRP, insulin, VLDL and triglycerides, an increase in HDL concentration, and a decrease in the HOMA-IR index compared with the separate use of melatonin and quercetin. [ABSTRACT FROM AUTHOR]

Background. Patients diagnosed with an ST-elevation myocardial infarction and type 2 diabetes mellitus present a complex clinical scenario with an increased risk of adverse cardiovascular outcomes. Despite advancements in the standard treatment approaches for an ST-elevation myocardial infarction, the mortality and morbidity rates among individuals with concomitant type 2 diabetes mellitus remain considerable. This study aims to compare the effects of adenosine and quercetin, both alone and in combination, with standard treatment on the outcomes in patients with an ST-elevation myocardial infarction and type 2 diabetes. Materials and methods. This study employed a prospective, randomized controlled design to compare the effects of adenosine, quercetin, and standard treatment in patients with an ST-elevation myocardial infarction and type 2 diabetes mellitus. A total of 97 patients were recruited and randomly assigned to three groups: group 1 (adenosine and quercetin), group 2 (quercetin), group 3 (standard treatment only). The patients were followed up for 3 weeks. Results. The combined treatment with adenosine and quercetin, along with standard treatment, demonstrated a more pronounced effect on improving cardiac function compared to quercetin and standard treatment alone. Specifically, the combined treatment group exhibited a significant reduction in ST elevation after percutaneous coronary intervention, indicating a more complete resolution of myocardial ischemia. Moreover, the combined treatment group showed improvements in echocardiographic parameters, such as left ventricular ejection fraction and global longitudinal strain, indicating enhanced cardiac function. Additionally, the combined treatment appeared to have a positive impact on quality of life, as assessed by validated questionnaires. Notably, a slight decrease in body mass index was observed in the combined treatment group, suggesting a potential benefit in weight management. Conclusions. The results of our study support the notion that the combination of adenosine, quercetin, and standard treatment may offer superior outcomes compared to individual therapies or standard treatment alone. This combined approach demonstrated a more substantial resolution of ST elevation, enhanced cardiac function, and improved quality of life. Furthermore, there was a suggestive indication of potential weight management benefits, as evidenced by a slight decrease in body mass index in the group receiving the combined treatment.

Background. Histamine is one of the versatile biogenic amines with multiple roles in the immune response and allergic disorders. Histamine and ATP can act as ligands in the body. In plasma, ATP is a potent vasodilator that stimulates the formation of NO and prostaglandins and, very importantly, can offset local sympathetic vasoconstriction. Adenosine triphosphate is released into plasma from erythrocytes and endothelial cells, and the plasma concentration increases in both the feed artery and the vein draining the contracting skeletal muscle. Taking this into account, it is important to study the effect of histamine in combination with quercetin, which inhibits the release of histamine from cellular depots, on the content of the superoxide anion and ATP in the blood plasma. Materials and Methods. Nonlinear white male rats were used for the experimental studies. Quercetin solutions were added to whole blood to a final concentration of 0.1; 0.3; 0.5; 1; 3; 5 mM. In other experiments, histamine solution was added to the blood to final concentration of 0.01; 0.1; 1; 10 μM. In a series of experiments, histamine (0.01 and 10 μM) and quercetin (0.1; 0.5; 3; 5 mM) were added to the blood in various possible combinations. Blood plasma was used in each experimental group. The blood to which saline was added was used as control. The content of superoxide anion and adenosine triphosphate was measured in the selected samples. Results and Discussion. Histamine, quercetin, as well as their combined action lead to the intensification of superoxide anion generation in the blood plasma of rats. Quercetin is known to be an antioxidant, but some of our studies have shown the opposite effect. Quercetin causes an increase in the ATP content in the blood plasma of rats. Histamine causes the same effect only at concentrations of 0.1 and 10 µM. Likewise, the combined administration of histamine and quercetin into the blood increased the ATP content. The results of ANOVA test prove that both histamine and quercetin have the same effect on the release of superoxide anion and ATP from blood cells into plasma. Their combined action only strengthens the effect of releasing the studied products. Conclusion. Quercetin, histamine and their combined action cause an increase in the generation of superoxide anion and ATP in the blood plasma of rats.

Aim. Determine the effect of quercetin on predictors of not reaching target blood pressure (BP) levels in patients with arterial hypertension (AH) and coronary heart disease (CHD). Materials and methods. 120 patients with stable coronary heart disease and hypertension of the II stage 2–3 degrees (66 women and 54 men) were examined. The patients were divided into 2 groups: Group I – 58 patients who, in addition to basic therapy (ramipril/amlodipine), received quercetin (Corvitin®), Group II – 62 patients who received only basic antihypertensive therapy. The parameters of daily blood pressure monitoring, blood lipid spectrum, C-reactive protein, the concentration of adhesion molecules (s-VCAM, s-ICAM-1), levels of endothelin-1 (ET-1), IL-1, IL-6, TNF-α were determined. Statistical processing of materials was carried out using the following programs: Minitab 21.0, StatPlus Pro 7.6.5, Wizard Pro, Jamovi, MedCalc. Results. We have established that the risk of not reaching the target level of daily SBP in the group of basic treatment of hypertension (group II) significantly increases with exceeding the level of LDL >4.0 mmol/l (by 3.6 times), an increase in the level of IL-6 >7 ng/ml (by 10 times), an increase in the level of TNF-α >9 pg/ml (by 7 times), an increase in the level of VCAM >1100 ng/ml (by 2.4 times). In the group of people who additionally took quercetin (group I), with an increase in the level of ET-1 >1.1 pg/ml, the risk of failure to reach the target SBP increases by 2.6 times, in the II group – by 3.5 times toward to daily SBP and 8.6 times – toward to daily DBP. We found out that an increase in the concentration of IL-6 > 7 ng/mg significantly increases the risk of arrhythmias by 6 times in group II. Conclusions. When quercetin is used, it is possible to achieve the target values of SBP (daily, daytime, nighttime) and DBP (daily, daytime, nighttime) in a significantly higher proportion of patients compared with those who are prescribed only standard antihypertensive therapy. Quercetin has an endothelial protective effect, as evidenced by a significant decrease in the levels of vascular endothelial adhesion molecules (s-VCAM), type I intercellular adhesion molecules (s-ICAM-1), ET-1, IL-1, IL-6, TNF-α. The additional use of quercetin against the background of standard antihypertensive therapy significantly reduces the risk of failure to achieve the target levels of SBP, DBP and the risk of heart rhythm disturbances even against the background of increased levels of proinflammatory cytokines and markers of endothelial dysfunction.

The aim of this work was to evaluate the effectiveness of quercetin addition to the treatment regimen for patients with COVID-19 associated pneumonia. Materials and methods. The effectiveness of two dosage forms of quercetin was studied in 200 patients, who were divided equally into the main and control groups. The main group patients received quercetin in addition to the basic therapy: intravenous drip of Quercetin/Polyvinylirolidone during the first 10 days followed by oral administration of Quercetin/Pectin over the next 10 days. Patients from the control group received only the basic therapy drugs. The study evaluated the dynamics of the disease symptoms (saturation level, respiratory rate, body temperature, cough, general weakness), as well as laboratory markers (C-reactive protein (CRP), ferritin, D-dimer). Results. Two dosage forms of quercetin consistently used in addition to the basic therapy improve pulmonary gas exchange and accelerate the lung function recovery. This is evidenced by a statistically significant majority of patients with positive dynamics in the symptoms of “Saturation level” and “Cough” as well as the meeting a complex indicator of the therapy effectiveness 2 days earlier than in the control group. The treatment regimen applied also helps to stabilize the level of D-dimer in the blood of the main group patients. Conclusions. The use of two dosage forms of quercetin in addition to the basic therapy accelerates the recovery of patients with coronavirus disease associated pneumonia and can help to prevent the progression of COVID-19 associated coagulopathy.

The article presents an assessment of the dynamics of changes in the content of the marker of collagenolysis – free oxyproline in the homogeniate of soft tissues and bone in experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on these indicators. The aim of this study was to determine the role of cytokinogenesis and the effect of flavonol on it in the pathogenesis, development and course of experimental periodontitis. During the experiment, a fragment of the mandible was taken from the animals, from which the soft tissues and bone were carefully separated. The state of collagen was determined by the content of free oxyproline in the soft and bone tissues. The concentration was determined according to the calibration graph and expressed in μmol/g. The results of studies of the indicators of the state of biopolymers of connective tissue structures of periodontium on the 7th, 14th and 30th day of experimental bacterial-immune periodontitis and after its correction with flavonol (from the 7th to the 14th day of the experiment) are presented. The data on the nature of changes in the content of collagen monomers in the process of formation of the inflammatory focus in the periodontal complex are given. During the acute phase of the inflammatory process in rats there was revealed a slight increase in blood free oxyproline in bone homogenate and homogenate of soft periodontal tissues, on the 14th day the dynamics continued to increase, at a later stage of the experiment, namely on the 30th day, increase in bone resorption continued as compared to the 7th and 14th day. During the correction of disorders resulted from the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14th day. The use of flavonol quercetin, which, by affecting immune processes, limited the inflammatory response in periodontal tissues and stabilized collagenolysis processes in periodontal tissues was manifested by a decrease in free oxyproline in bone and soft tissue homogenates of experimental animals.

Aim: To investigate the effectiveness of course quercetin in improving functional state of the microvascular endothelium and to determine the possibility of using quercetin to increase body resistance to hypoxia in the elderly. Materials and methods. The effectiveness of quercetin in improving the functional state of the endothelium and increasing body resistance to hypoxia was studied in 110 elderly patients with metabolic syndrome, who were divided equally into the main and control groups. Patients in the main group received quercetin at a daily dose of 240 mg for 3 months, and patients in the control group received placebo for the same period. Before and after treatment, the microvascular endothelial function was studied by laser Doppler flowmetry using the post-occlusive hyperemia test. Acute hypoxic test was performed under isocapnicnormobaric hypoxia, during which the followings were recorded: blood saturation (SpO2), heart rate and diastolic blood pressure (BP). Results. In the group of patients receiving quercetin, there was an improvement in blood flow to the skin, as evidenced by a statistically significant increase in the maximum volumetric rate of cutaneous blood flow and the duration of the recovery period of OSHSK to baseline in the post-occlusive hyperaemia. The course of quercetin also led to a less significant decrease in SpO2 during hypoxic test and to a decrease in the absolute values of systolic and diastolic blood pressure, while reducing an increase of the systolic blood pressure on the 20th min of the hypoxic test. Conclusions. In elderly patients with metabolic syndrome (within a 3 month course) the use of quercetin has improved the microvascular endothelium functional status and increased the resistance to hypoxia.

Aim. To compare the nephroprotective effect of oral applications of different phytogels in experimental kidney pathology.Methods. We used phytogels: "Kvertulin" (quercetin + inulin + calcium citrate), "Biotrit" (juice from wheat germ) and "Dubovy" (extract from oak wood).Experimental models were reproduced in rats: diabetic syndrome, prednisolone immunodeficiency, intoxication with thermal peroxide sunflower oil. The condition of the kidneys was assessed by changes in the level of biochemical markers in the kidney tissue (elastase, urease, lysozyme, MDA, the degree of dysbiosis).Results. Experimental pathologies cause an increase in the level of elastase, urease, MDA, the degree of dysbiosis, and a decrease in lysozyme activity in the kidneys. Oral application of phytogels caused a decrease in the activity of elastase, urease, MDA content and the degree of dysbiosis, but increased the activity of lysozyme. When recalculating the degree of decrease in elastase activity and increase in lysozyme activity per 1 g of phytogel or 1 mg of active principle (quercetin or the amounts of polyphenols), phytogel "Kvertulin" turned out to be the most effective anti-elastase agent, and phytogel "Biotrit" turned out to be the most effective lysozyme-reducing agent.Conclusion. Oral applications of multifunctional mucous-adhesive gels have a nephroprotective effect in experimental kidney pathology.

The search for safe and effective drugs that can influence the course and correction of metabolic syndrome, especially in the older age group, has drawn attention to the possibilities of using an antioxidant from the bioflavonoid group, quercetin. The search for literature data indicates the absence of clinical studies on the use of quercetin in order to increase resistance to hypoxia in elderly people with metabolic syndrome. The aim of the study. To establish the response to an acute hypoxic test in the elderly people with metabolic syndrome after a course of quercetin in comparison with the control group. The object was 110 elderly patients with metabolic syndrome criteria, of which 55 people took quercetin (drug “Kvertin” PAO SPC «Borshchahivskiy CPP», 80 mg three times a day for 3 months), and 55 people took placebo and formed a control group. Methods. Anthropometric measurements; biochemical study with determination of glucose in blood plasma, lipids in blood serum; measurement of blood pressure; hypoxic test to determine resistance to hypoxia. Results. The course use of quercetin led to a less significant decrease in SрO2 in the main group of patients during the hypoxic test. Also, in the main group, after treatment, there was a statistically significant decrease in systolic and diastolic blood pressure, both at the beginning of HP and at 20 minutes of HP compared to the baseline level and the control group. In addition, there was a statistically significant decrease in the double product – an indicator that indirectly characterizes the level of oxygen consumption by the myocardium in the main group compared to the control group. Conclusions. Course use of quercetin significantly increases the body’s resistance to hypoxia in elderly patients with metabolic syndrome. The antihypoxic effect of quercetin can be used to correct conditions associated with oxygen deficiency.

The purpose of this study is to investigate the effects of bioflavonoids, epigallocatechin-3-gallate (EGCG) and quercetin, on the production of reactive oxygen and nitrogen species in the liver of rats under round-the-clock light exposure with an intensity of 1500 lux for 30 days while being kept on carbohydrate-lipid diet (20% fructose solution and appropriate food) for 60 days. In the liver tissues, the rate of the superoxide anion production by NADPH-dependent electron transport chains (microsomal monooxygenases and constitutive uncoupled NO synthases) increased by 1.93 times, by the mitochondrial respiratory chain by 1.89 times, and it was doubled by leukocyte NADPH-oxidase. The total activity of NO synthase was increased by 2.35 times, the activity of its inducible isoform increased by 2.57 times, and the concentration of alkali and alkaline earth metals peroxynitrites elevated by 1.68 times. Administration of bioflavonoids-epigallocatechin-3-gallate and quercetinsignificantly restrained the rate of superoxide anion production in the liver tissues by microsomal monooxygenases and NO synthase by 39.1 and 40.1%, by the mitochondrial respiratory chain by 37.2 and 34.4%, by leukocyte NADPH-oxidase by 35.0 and 32.1%, respectively. Epigallocatechin-3-gallate and quercetin inhibited the total activity of NO-synthase by 46.7 and 36.2%, the activity of its inducible isoform by 49.6 and 39,0%, increased the activity of the constitutive isoenzyme NO-synthase by 2.9 times and its coupling index by 4.5 and 4.7 times. Additionally, administration of these bioflavonoids lowered the concentration of peroxynitrites of alkali and alkaline earth metals by 30.5 and 34.3% compared to the respective values obtained in the group of rats, which did not receive the bioflavonoids, but were exposed to light and carbohydrate-lipid-rich diet. We suggest that epigallocatechin-3-gallate and quercetin in the above experimental conditions are effective means to restrain the formation of reactive oxygen and nitrogen species in the liver tissue. [ABSTRACT FROM AUTHOR]

In the absence of effective and safety therapy for COVID-19 patients, today the search for new methods of treatment is the most actual topic in medicine and pharmacy. High mortality (7.1 %) due to the development of acute respiratory distress-syndrome, which is caused by cytokine storm, dictate the need to develop a new approaches to the influence on a various pathogenetic links of the disease in the complex therapy of such patients. This review summarizes the data of experimental and clinical studies of the pharmacological properties of quercetin (mechanisms of action, pharmacological effects) and quercetin-containing drugs Quertin and Corvitin (oral and parenteral dosage forms), which are presented on the pharmaceutical market of Ukraine. The results of numerous studies indicate that quercetin has (among others) high anti-inflammatory, antiviral, membrane-stabilizing, immunomodulating and antioxidant effects. The mechanisms for the realization of these pharmacological effects are well studied and make it possible to use quercetin as a pathogenetic therapy (effect on the various pathogenesis links) in patients with coronavirus infection. This dictates the need for the conducting of appropriate clinical trials of these drugs for the prevention and treatment of coronary infection. Clinical trials results may be an innovative strategy in the treatment of COVID-19 patients.

The article features the diet therapy in hypertension, recommendations of the World Health Organization (WHO), leading European nutritionists and cardiologists on the disease. The aim of the study – to get acquainted with the nutritional characteristics of hypertension; analyze known publications on nutrition and diet therapy in hypertension. Also, changes in lifestyle are recommended for the prevention and treatment of hypertension. Cardiovascular disease is one of the leading causes of morbidity and remains one of the major causes of mortality in economically developed countries. One of the most serious health problems today is arterial hypertension, a leading risk factor for myocardial infarction and stroke, as well as the cause of disability and death among people of working age. WHO experts from the 12 largest countries in the world have said that about 20 % of the adult population of the planet suffers from high blood pressure, hypertension "is younger" in recent decades. In this regard, according to researchers, pressure is reduced by 19 % in boys and 16 % in girls. Therefore, the problems of further improving the treatment and prevention of arterial hypertension are extremely relevant and have become the subject of numerous studies, including the areas of nutrition and diet therapy. At present, the main factors of food risk for hypertension are excessive intake of sodium, saturated fatty acids, excess and caloric diet, etc. The article gives recommendations for changing the way of life for the prevention and treatment of hypertension. Fatty foods, stomach overload with food, high salt content, water retention in the body, and as a result of increased pressure, lead to an increase in the number of cardiovascular diseases. This review of the literature describes and examines the nutritional features of different countries of the world, examines the effects of diet therapy and provides recommendations for improving the health of the population: limiting sodium intake (no more than 5 g/day); reduce the total amount of liquid (up to 1–1.2 liters per day); maintaining adequate potassium consumption (through fresh fruits and vegetables); consumption of sufficient amounts of calcium, magnesium and other minerals; food fibers; enriching your diet with seafood; slimming (Mediterranean diet with moderate use of red wine); reduction or complete refusal to use foods rich in caffeine; flour products, semi-finished products, etc.; increase in physical activity (walking and running in the fresh air, swimming, cycling, exercises on a bike). Conclusions. Proper nutrition in hypertension is one of the important ways to normalize the functions of the heart and blood vessels, thereby improving the quality of life without leading to fatal results. Nutrition should help to maintain health, meet the needs of the body in nutrients, calories, as well as vitamins and mineral elements.

Copyright of International Journal of Endocrinology / Mìžnarodnij Endokrinologìčnij Žurnal is the property of Zaslavsky O.Yu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The purpose of this work was to determine the patterns of quercetin and rutin sorption on the aluminium oxide as dependent on concentration and pH of their water-ethanol solutions to develop the assays of the sorptionspectrophotometric determination of these natural flavonoids in preparations of plant origin. Complex adsorption and spectral studies of the interaction of quercetin and rutine with pyrogenic aluminium oxide in a water-ethanolic medium have been carried out. The flavonoid sorption on aluminium oxide starts at pH > 2.5 and reaches the maximum values in a neutral medium, correlating with the content of non-dissociated surface ≡AlOH groups. Both quercetin and rutin sorption is described with the H-type isotherms which is typical for the chemisorption. The found coincidence of spectral characteristics of flavonoids on the aluminium oxide surface and in solutions containing Al(III) ions indicates the formation of similar chelate complexes in these systems. The spectral characteristics (intensity and position of the absorption bands) of flavonoids adsorbed on the surface of aluminium oxide depend on their concentration and pH of aqueous solutions. The intensity of sorbent colour is proportional to the surface concentration of quercetin and rutin and values of their conditional molar absorption coefficients in 0.025 % alumina dispersion were determined (εS 434 = 4386 g/mol∙cm and εS 403 = 5280 g/mol∙cm respectively). In addition, the absorbance of such dispersions linearly depends on the flavonoid concentration in analyzed solutions. Thus, aluminium oxide is found to be a promising solid-phase reagent for the determination of quercetin and rutin in preparations of medicinal plants by spectrophotometric or visual test methods. [ABSTRACT FROM AUTHOR]

Copyright of Mezdunarodnyj Nevrologiceskij Zurnal is the property of Zaslavsky O.Yu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of the study was to evaluate the effectiveness of anemia's correction in patients with chronic heart failure of ischemic genesis with iron's drugs in comparison with their combination with quercetin based clinical, cardiohemodynamic characteristic, indicators of erythropoiesis and cytokines. Materials and methods. Tere were examinated 120 patients with chronic heart failure due to iscemic heart disease and anemia. Chronic heart failure FC II diagnosed in 49 pations, III FK – 42 and FC IV – 29. Patients with CHF and anemia were divided in 2 groups. 1 group recieved standart therapy and iron’s drugs, the 2 group was additionally assigned quercetin. Hemoglobin, serum iron, erythropoetin and ferritin levels were evaluated. Results. After the therapy, the level of erythrocytes, hemoglobin, hematocrit, serum iron in the first group increased by 5.3, 20.3, 12.8, 40.2 %, respectively, in the II group – by 9.2, 25.6, 14.2 and 62.4 % respectively. The level of erythropoietin before the onset of therapy in both groups significantly exceeded the control indices. After treatment it significantly decreased in the I group by 20.4 %, in II – by 28.2 %. A significant increase of the level of tumor necrosis factor-α, interleukin-1β was determined in both groups of patients with chronic heart failure and anemia, In first group level of tumor necrosis factor -α significantly decreased by 14.2 %, interleukin-1β – on 6.5 %, in II group – by 26.6 % and 28.1 % respectively after the treatment. In the first group the end-diastolic diameter was significantly decreased by 7.7 %, the end-diastolic volume by 9.5 %, the end-systolic diameter by 7.9 %, the end systolic volume by 7.5 %, the ejection fraction increased by 14.4 % and indicators of a 6-minute walk test by 19.4 %, quality of life parameters by 24.2 %, in II group the end diastolic diameter decreased by 11.3 %, the end diastolic volume by 10.2 %, the end systolic diam, eter by 9.4 %, the end systolic volume by 13.6 %, the ejection fraction increased by 18.3 %, the distance of 6-minute walk test improved by 34.0 % the quality of life parameters by 32.9 %. Conclusions. It has been detected, that anemia’s correction in patients with CHF, who received combination of standart treatment with iron’s drugs accompanied by an increase in the level of erythrocytes, hemoglobin, iron, and decreased level in the initially increased levels of erythropoetin, ferritin, tumor necrosis factor-α, interleykin-1β. Favorable changes in erythropoiesis were accompanied by a positive dynamics of the morphofunctional parameters of the myocardium, the distance during the 6-minute walk test and the quality of life indicators. Quercetin, included in the scheme of therapy of patients with chronic heart faiulure and anemic syndrome, potentiated hematopoietic and hemodynamic effects of therapy.

Objective – to study the effectiveness of Allopurinol and Quercetin inclusion in a complex treatment of stable angina pectoris and peculiarities of biomarkers and homeostatic indices changes. Materials and Methods. 120 patients with the diagnosis of stable angina pectoris (SAP) and hyperuricemia (HU) were examined and three groups were formed: patients receiving a complex standard therapy (70 persons – 58,33%), patients receiving the standard therapy with addition of Allopurinol (23 persons – 19,17%), and patients receiving Quercetin in addition to the standard therapy (27 individuals – 22,50%). At the beginning of in-patient treatment and 6 months later during out-patient period all patients were clinically and laboratory examined (uric acid, total cholesterol (TC), triglycerides (TG), creatinine, NT-proBNP, С-reactive protein (СRP) and total testosterone (TT) in the blood serum). Results. Reduced content of TC and TG in the 1st, 2nd and 3rd groups of a complex treatment was found (р

Objective - to study neuroprotective effect of Quercetin in ischemia-reperfusion injury in acute ischemic stroke. Materials and methods. The study included 98 patients with acute ischemic stroke. All patients: main and control group, received standard treatment in accordance with the clinical protocol order Ministry of Health of Ukraine from 03.08.2012, № 602. Patients of the main group (n=68) on the back of the base further treatment was administered quercetin (Corvitin lyophilisate injection solution) course of 10 days according to the scheme: 500 mg of the drug diluted in 100 ml of 0.9% of the physiological solution intravenously twice a day for the first five days and once a day for the next five days. Patients in the control group (n=30) - quercetin is not appointed. Assessment by GCS, NIHSS, Barthel served in the 1st, 3rd, 5th, 10th day of the disease. Results. Simultaneously with the standard treatment, intravenous administration of quercetin, positively influenced the regression of focal neurological symptoms on the NIHSS and Barthel scales in patients with acute ischemic stroke, increased the proportion of patients in the consciousness or with its minor impairments in the GCS, ie contributed to an earlier “awakening” in acute ischemic stroke. Conclusions. Neuroprotective effect of quercetin (Corvitin lyophilisate injection solution) can be explained by its polytropic, antioxidant, anti-inflammatory, membrane-stabilizing effect in ischemia-reperfusion.

The paper presents original data on improvement of effectiveness of treatment (reduction of systemic inflammation, stabilization of lipid metabolism, improvment of quality of life) in patients with coronary artery disease (CAD) and metabolic syndrome (MS), who underwent primary percutaneous coronary intervention (PCI) for acute coronary syndrome without ST-segment elevation (ACS nST) by adding quercetin to basic therapy, 40 mg 3 times a day during the 12,07 ± 1,51 months. The reduction of a high sensitive C-reactive proteins (hs-CRP), (2,51 ± 0,93, nmol / L., versus 3,12 ± 0,43, nmol / L.), (p = 0,0007) and low-density lipoproteins (LDL), (4,01 ± 0,36, mmol / L., versus 4,29 ± 0,71, mmol / L.), (p=0,049) in the main group II (n = 33) as compared with the control group I (n=31), respectively,as well as thrombin: 5,96 ± 1,89, ng / mL. in group I as compared with 3,91 ± 1,43, ng / mL. in group II, (p=0,0002) was revealed. High density lipoproteins (HDL) were significantly greater (p = 0,049) in the group II (1,35 ± 0,41, mmol / L.) in comparison with the group I (1,17 ± 0,34, mmol / L.).

Aim. Coronary artery disease (CAD) with concomitant metabolic syndrome (MS) leads to early disability and poor prognosis after urgent percutaneous coronary intervention (PCI) for acute coronary syndrome without elevation ST (ACS noST). Mathods and results. With the aim of treatment effectiveness improvement, 64 patients were treated (M : F = 55 : 9, mean age 66.9±12.4 years) in two regimens – standard (group I) and with metabolic supplement (group II). Patients in groups didn`t differ in the major clinical, anamnestic, laboratory and instrumental parameters. It was found, that in the II group (n= 33), those patients received in addition to the standard therapy quercetin, 40 mg three times daily for 12.07±1.51 months, compared with the group I (n=31), number of patients with MS` criteria decreased to 48.5% (n=16) and 77.4% (n=24), respectively. These results were obtained by reducing the waist circumflex of patients in the group II compared to the group I (p=0.034) and body mass index (p=0.048); improvement of insulin resistance (calculated by index HOMA) in group II patients in comparison to the group I (p=0.049), fasting blood glucose (p=0.024), low density lipoproteins (p=0.049) and plasma thrombin (p=0.0002). High density lipoproteins were significantly higher in group II patients compared to the group I (p=0.049). Conclusions: Long-term quercetin use in addition to standard therapy in patients with CAD and MS after PCI on the ACS without ST leads to the improvement of the system of metabolism due to the correction of the parameters of the metabolic syndrome.

The relevance of the work lies in the research of the quercetin and lipoate effect in terms of experimental diabetes treatment. Objective. To study the quercetin and lipoate effect on the glutathione system in retina in terms of diabetes progress process (in two and six months). Materials and methods. The research was conducted using albino rats. Experimental animals were divided into four groups: the first one – control group (14 rats); the second one – experimental group (14 rats), untreated animals with developing diabetes; the third one – experimental group (12 rats), animals with developing diabetes and lipoic acid administration; the fourth one – experimental group (15 rats), animals with developing diabetes and quercetin administration. Reduced and oxidized glutathione content was determined in retinal homogenates. Results. Lipoic acid and quercetin use enhanced the reduced glutathione level by 107.2 and 84.3% in 2 months in terms of research, and by 134.8% and 113.0 in 6 months after the diabetes progress compared to the group of untreated animals. Conclusion. In terms of streptozotocin-induced diabetes progress the decrease of coenzyme (reduced) glutathione form by 61.9% (in 2 months) and by 68.3% (in 6 months) was determined. Lipoic acid and quercetin medical products administration had a normalizing effect on the retinal metabolism, which is manifested in the reduced glutathione levels increase by 107.2 and 84.3% (in 2 months) and by 134.8 and 113.0% (in 6 months). The lipoic acid application creates a more distinct normalizing effect on the glutathione level.

Kidneys perform excretory function and regulate the work of many systems of the body, therefore, the morphological changes of the functional apparatus of the kidneys must necessarily affect the functions of other organs and systems. Data on pathological processes in the kidneys caused by the action of ethanol are diverse and can be manifested as universal tissue reactions to the action of ethanol and manifestations of its toxic effects directly on the tissue of the kidney. Aim: to study the morphological changes in kidney tissues of rats with acute ethanol-induced injury and correction with quercetin and L-arginine L- glutamate. Experimental studies performed on 66 white non-linear, sexually mature male rats weighing 120-130g (average weight - 125 g). Acute alcoholic defeat was modeling by intragastric administration of 40% ethanol in a dose of 20 ml/kg for 7 days with the help of a metal probe with oil. The revealed changes in renal tissue can be considered valuable morphological criteria that characterize the damage of the renal parenchyma in acute alcohol defeat. Using of the preparations of quercetin and L-arginine L-glutamate promotes the protection of kidney cells from the toxic effects of ethanol, which manifested itself in decreasing the severity of dystrophic changes in the endothelium of the tubules. The performed morphological study allows verifying more pronounced tread effect in the form of improving the histostructure of the kidneys under the combination of both drugs in comparison with monotherapy.

Introduction. The thesis actuality consists in studying the quercetin and lipoate effect during experimental diabetes treatment. Objective. To study the quercetin and lipoate effect on the antioxidant system enzymes, particularly, glutathione peroxidase and glutathione reductase during experimental diabetes. Material and methods. Researches were conducted on white rats. Experimental animals were divided into four groups: the first one – control group (14 rats); the second one – experimental group (14 rats), animals with developing diabetes, without drug administration; the third one – experimental group (12 rats), animals with developing diabetes and lipoic acid administration; the fourth one – experimental group (15 rats), animals with developing diabetes and quercetin administration. The activity of glutathione peroxidase and glutathione reductase was determined in homogenates of retinas and blood plasma. Results. The usage of thiol preparations and bioflavanoid considerably contributes to the stabilization of hydroperoxide neutralization processes in the retina in conditions of experimental diabetes. It is also required to specify that the effect of a thiol preparation – lipoic acid was more expressed as compared with quercetin. Conclusion. The effect of lipoic acid and quercetin preparations during the experimental diabetes (2 & 6 months) considerably allows avoiding the inhibition of antioxidant system enzymes in animal retina. It is confirmed by the increase of the activity of glutathione peroxidase in the retina when using lipoate by 65.0% (in 2 months) and by 74.3% (in 6 months), and glutathione reductase - by 26.7% (in 2 months) and by 34.4% (in 6 months) for the group of untreated animals. The quercetin injection increases the activity of glutathione peroxidase by 60.6% (in 2 months) and by 65.9% after six months, and glutathione reductase by 20.4 and 29.8% correspondingly. The most expressed effect for reducing the degree of damage of antioxidant system enzymes is specific for lipoic acid.

Background. Since NSAIDS have some detrimental side effects, studying new combinations of NDAIDS with other substances could provide a reduction of the dosage of the NSAID while retaining therapeutic effectiveness. Objective. To study the effect of Diclocor on the cartilage morphology in the course of collagen-induced arthritis in rats in comparison with sodium diclofenac and quercetin. Methods. Autoimmune arthritis was reproduced by subcutaneous injections of type II collagen ("Sigma-Aldrich", USA). Morphological studies were performed using standard light microscopy techniques. Results. After treatment with both Diclocor and diclofenac sodium no cartilage destruction was oberved and tissue hyperplasia was low. Some residual effects were observed in the synovial membranes in the Diclocor group, and in periarticular tissues – in the diclofenac sodium group. Under the action of quercetin, there was a sufficiently high level of hyperplastic processes and pannus formation in sinovium. Conclusion. Diclocor and diclofenac sodium express a profound normalizing effect, while Quercetin is less active. Citation: Popov OS, Zupanets IA, Shebeko SK. [Morphological study of the effect of the drug "Diclocor" in the course of collagen-induced arthritis in rats]. Morphologia. 2015;9(3):58-66. Ukrainian.

Aim. In the complex treatment of diseases that are associated with the stress or the overfatigue, much attention is paid to the drug therapy. On the Ukrainian pharmaceutical market synthetic drugs dominate among other drugs of tonic effect. The aim of our research is to develop chewable tablets with dry oats extract and quercetin. Methods and results. During our work qualitative and quantitative composition of chewable tablets as well as conditions of granulation and tableting processes have been substantiated, the technological process for preparing solid dosage form has been developed, technological flowchart has been composed. Conclusion. This makes possible to improve composition and technology, and to expand the spectrum of pharmacological effects of the known drug Kvertin (PAO SPC «Borshchahivskiy CPP»).

Quercetin and rutin have potent antioxidant and metal ion chelating capacity, possess various biological, biochemical and therapeutic activity, but their application is limited because of low solubility. Anticeptic cationic gemini surfactant ethonium (2-ethylene-bis-(N-dimethylcarbdecyloximethyl) ammonium dichloride) was considered to be a promising solubilizer for flavonoids. The supramolecular interactions of quercetin and rutin with ethonium have been studied spectrophotometrically. The bathochromic shift of quercetin and rutin spectra in pre-micellar ethonium solutions testifies an increase in their polarity. Indeed, the calculated values of quercetin and rutin apparent dissociation constants in ethonium solutions are lower than that in water. In micellar solutions the flavonoid spectral characteristics and protolytic properties remain unchanged, whereas the solubility of quercetin and rutin raises 250 and 5 times, respectively. The binding constants of quercetin (lgKb = 3.71) and rutin (lgKb = 2.52) with ethonium are determined from the solubility data. The formation of supramolecular complexes also causes the essential increase of quercetin and rutin adsorption on silica surface at рН > 5 both in pre-micellar and micellar ethonium solutions in similar ways. This allows us to assume that the structures of surface complexes formed by pre-micellar associates or micelles have no principal differences. The shape of the obtained pH-dependences of quercetin and rutin adsorption suggests that flavonoids as a part of ethonium supramolecular complexes adsorb on silica mainly through the ionic interactions between the positively charged nitrogen atoms and dissociated silanol groups. The results of physico-chemical study may be a base for the development a composition of new medicinal remedies. [ABSTRACT FROM AUTHOR]

Effect of quercetin, epigallocatechin gallate and polyphenol extract from grapes administration on the changes in cholesterol metabolism in the rat blood serum under insulin resistance was studied. The development of atherogenic dyslipoproteinemia has been revealed: the accumulation of total cholesterol (2.12 times) due to the increase of very low density lipoproteins cholesterol content (1.75 times), the decrease of high density lipoproteins cholesterol concentration (2.6 times) and the increase of the atherogenic index (8.8 times) in animals on a high-fructose diet. The use of all studied polyphenols normalized the indicators of cholesterol metabolism in a different degree. Polyphenolic grape extract showed more pronounced protective properties, obviously due to the variety of composition and, accordingly, the mechanisms of insulin resistance correction. [ABSTRACT FROM AUTHOR]

The influence of treatment with quercetin inclusion on the clinical and biochemical course and the cytokine profile of chronic nonviral hepatitis patients have been studied. In addition to the basic therapeutic treatment quercetin intake led to the decrease in the cytolytic and cholestatic syndromes activities which were manifested by a faster regression of clinical signs of the disease. During two weeks of treatment chronic hepatitis patients who received quercetin in addition to standard treatment, showed decrease in proinflammatory tumor necrosis factor-α by 61,9% (p = 0,02) and a marker of early cardiovascular injury atrial natriuretic propeptide in the blood by 53,8% (p = 0,04). However, according to the data obtained, it is clear that for a complete correction of clinical manifestations of the disease, biochemical changes and the cytokine profile two-week appointment of complex treatment with quercetin inclusion is not enough, which requires longer administration of the chosen treatment course before the onset of persistent remission at the out-patient stage.

Aim: To compare action of quertulin and adrenoblocators oral geles on the biochemical indices of rat serum after common action adrenaline and lincomycin. Methods: Adrenaline stress was made by oral application of gel with adrenalin in dose 0,36 mg/kg during 10 days. Lincomycin was introduced with drinking water in dose 60 mg/kg. The gel of quertulin was introduced by application in dose 20,34 mg/kg and the gel of adrenoblocators (zocson + nicergolin and sibason) by application in dose 0,6 mg/kg. Conents of glucose, triglycerides, total cholesterine and malonic dialdehide (MDA), the activities of urease, lysozyme, elastase and catalase were determined into serum. Results: Both geles no influenced on the serum conten glucose and triglycerides, but raised the content cholesterine. The adrenoblocator gel reduced activity urease and content MDA, but raised activity catalase. The quertulin gel reduced the activity elastase and raised the activity catalase. Conclusion: Oral application of gel with adrenoblocators made antidysbiotic action more then quertulyne, but yielded in antiinflammation and antioxidative actions to quertulyne after common introduce adrenaline and lincomycin.

The purpose of this research was to study the correction by the quercetin of the structural and functional changes in the mucous membrane of the mouth cavity of rats caused by the action of the doxaglide prooxidant and chronic emotional and painful stress in conditions of nutritional deficiencies in plant components. The research was conducted on 21 rats 1.5 months. age. The duration of the experiment was 60 days. Intact group (1) consisted of 7 rats, which were kept on a standard ration of the vivarium. In the 2nd group (7 rats), after 1 month of their non-polifenol diet were subjected to the combined effects of the delagil oral injections (5 mg / kg) and chronic emotional and pain stress. The protective effects of the quercetin have been studied with the combined effects of delagil, chronic stress and non-polifenol diet. Rats of the 3 group (7 animals), aftewr one month from the beginning diet were orally receiving quercetin (50 mg / kg) five times per week for 30 days. The combined effect of the epitheliotropic toxicant delagil, a chronic emotional pain syndrome with alimentary polyphenolic insufficiency, caused pronounced dystrophic changes in the epithelium and the intrinsic plate of the oral mucosa. Quercetin was the best corrector of inflammatory-destructive changes. The nature of the compensatory-regenerative processes in the cells of the epithelial layer acquired features inherent in the intact group. Quercetin prevented the oppression of proliferative activity of the epithelium.

Analysis of the domestic and foreign literature sources devoted to the study of pathogenetic mechanisms of gastropathy caused by non-steroidal anti-inflammatory drugs was done. Current approaches of prevention and treatment of NSAID-induced gastropathy were lined. The appropriateness of drugs with polytropic pharmacological properties (Quercetin, Vinboron and Tiotriazolin) to eliminate the side effects of NSAIDs, including ultserogenesis was discusses.

It is known that endothelial dysfunction is one of the major factors in development of diabetic cardio-vascular complications. Besides, pathophysiological linkages between high concentration of glucose and vascular dysfunction remain not fully established. The aim of this study was to investigate acetylcholine-induced endothelium-dependent dilation of vascular segments obtained from healthy rats and incubated in hyperglycemic solution and vascular segments from diabetic rats before and after quercetin-filled phosphatidylcholine liposomes (Lipoflavon®) treatment in both in vitro and in vivo conditions. Changes in vascular tone were registered in isometric mode. Data are presented as mean ± SEM with “n” indicating the number of vascular preparations tested. On the day of the experiment diabetic rats showed four times higher blood glucose level and decreased dilation responses to acetylcholine (ACh, 1 nM–10 mM) of aortic rings precontracted with noradrenaline (NA, 1 mM)as compared to controls. Incubation of vascular rings under hyperglycemic (25 mM/L glucose) conditions led to time-dependent impairment of dilation responses as compared to normoglycemic (5.5 mM/L glucose) conditions. Lipoflavon® (3 µg/mL of quercetin) application to the organ bath with diabetic rats precontracted vascular rings resulted in a significant reduction in vascular tension. Being added on the plateau of Lipoflavon® relaxation, ACh showed increased dilation responses in comparison to diabetic tissues without Lipoflavon® application. Intravenous Lipoflavon® injections to diabetic rats in concentrations 15 mg/kg or 50 mg/kg of phosphatidylcholine restored dilatation responses up to the control level. In conclusion, we confirmed that hyperglycemia is one of the main factors leading to impairment of endothelium-dependent vasodilation. Quercetin-filled phosphatidylcholine liposomes significantly improve endothelium-dependent relaxation of isolated vascular rings from diabetic rats. We assume that Lipoflavon® is perspective in treatment of diabetic angiopathies.

This experiment was aimed at studying the effect of combined action produced by L-arginine and water-soluble form of quercetin (corvitin) on the markers of oxidative-nitrosative stress in the skin under 30-day intoxication with sodium nitrate (200 mg/kg per day). The combined intraperitoneal administration of L-arginine (500 mg/kg) and corvitin (10 mg / kg in terms of quercetin), once every three days starting from the 15th day of intoxication, reduced the formation of peroxynitrite, production of superoxide anion radical (.О-2) by NADPH-dependent (endoplasmic reticulum and NO-synthase) and NADH-dependent (mitochondrial) electron transport chains in the skin tissues, decreased the concentration of compounds reacting with thiobarbituric acid products during incubation in the prooxidant iron-ascorbate buffer solution. The values of these indicators were significantly inferior to the corresponding data obtained by the separate administration of these substances. The combined effect of L-arginine and quercetin in the experimental conditions increased the activity of arginase and superoxide dismutase in the skin tissues, that significantly exceeded the results of the series, in which above mentioned compounds were used separately. We can conclude the combined administration of L-arginine and corvitin is more effective in preventing oxidative-nitrosative stress in the skin of rats exposed to excessive intake of sodium nitrate compared with their separate application, and reduces the production of reactive oxygen and nitrogen species (.О-2 and peroxynitrite), increases the antioxidant potential, and enhances the activity of arginase and superoxide dismutase. [ABSTRACT FROM AUTHOR]

Copyright of Problems of Environmental Biotechnology is the property of National Aviation University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction. The working out of the medicines on the basis of natural bioflavonoids, quercetin in particular, is a perspective direction of broading doctors’ remedy arsenal with polytropic effect. Quercetin has different pharmacological aspects: anti-inflammatory, antioxidant, radioprotective effects and has elements of cardio-, nephro-, gastro- and hondroprotection. Materials and methods. The object of the research is quercetin substance. Microscopic analysis has been held with the help of laboratory microscope «Konus-Akademy» with ocular-camera Scope Tek DCM510. To visualize the pictures software Scope Photo™ has been used, that allowed to measure the line sizes in real time condition and create stative picture. To define the form the roundedness parameter definition has been used, which can be found as ratio of circumference length with the same area as a particle to the factual particle perimeter. The cosine of angle soaking has been researched according to the dynamics of liquid penetration into the substance within 10 minutes. Results and discussions. The results of microscopic analysis indicate that quercetin substance refers to crystalline monoclinical system. The particles have anisodiametrical form with the fragments on the surface. Form factor is 0,2. The roundedness parameter is approaching 0. The particles are able to agglomerate. The definition of not crushed substance fraction composition by means of sieve analysis showed the advantages of 1 mkm particles. The use of microscopic method allowed to establish that the main particle size of not crushed substance vacillates in the interval 1-0,5 mkm, crushed is 0,5-0,25 mkm. The vacillation of the particle size depending on definition method could be explained by the presence of electrostatic power connections between quercetin particles. According to the received dissolvation data quercetin can be referred to the fourth class of biovailability. It allows to affirm the reason of dissolubility raise of this substance. Quercetin soaking research exposed that the substance does not get wet with hydrofoil dissolutions. Melting temperature determination showed that quercetin refers to thermostable powders (Тmelt. – 302 0С), that is why there are no physical-chemical changes while mechanic and physical affect. Thickness meaning (1,478) gives a chance to predict the creation of substance particles conglomeration which is being investigated. Conclusion. Thus, received experimental data allows to come to conclusion about the necessity to carry out the researches of the physical-chemical quercetin substance features to improve biopharmaceutic indices.