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Aim. This study was aimed to examine influence of сytokinin fraction of basidiomycete Hericium coralloides on the spontaneous and induced phagocytic activity of murine peritoneal macrophages. Materials and methods. Mononuclear phagocyte (macrophage) fraction of peritoneal exudate of C57BL/6 mice was used. Macrophages were incubated under standard conditions at 37 °C, 100% humidity and 5% CO2 for 4 hours. Phorbol 12-myristate 13-acetate (PMA) was added to part of the samples to activate oxidative metabolism. Hericium coralloides was added in two concentrations that were 5 and 10 times lower than the IC50, defined as an antiproliferative effect on colon cancer cells. Incubation with samples was carried out for 2 hours. Results. When adding PMA, Hericium coralloides (0.017 and 0.035 ?g/ml) and in the combination of PMA with Hericium coralloides, activation of reactive oxygen species (ROS) in peritoneal macrophages was revealed by 1.37-1.7 times, compared to the spontaneous activity of phagocytes. Conclusions. Thus, the effect of the cytokinin e.tract of the basidium fungus Hericium coralloides was manifested by an increase in the phagocytic activity of peritoneal macrophages as one of the possible mechanisms of immunomodulatory action.

The intensities of oxidative metabolism and nitric oxide production in the trained and untrained female students aged 18-22 years under adaptation to physical load were studied. At the initial stage of the competitive period in the trained students adaptive rearrangements were accompanied by a significant increase in the intensity of the oxidative metabolism (an increase in the rate of generation of the superoxide radical, hydroxyl radical, uric acid content, hydrogen peroxide), production of nitric oxide (increased activity of the constitutive NOS and decrease in the content of the marker of peroxynitrite - nitrate anion) and lipid peroxidation. The growth of the content of TBA-active products indicates the intensification of these processes. High (r>0.70) correlation dependence among trained girls students between the functional state of the organism and the indicators of the intensity of oxidative metabolism and nitric oxide production was revealed. Our results point for the important role of these processes in the formation of adaptive changes in the body under systematic physical load. [ABSTRACT FROM AUTHOR]

The intensities of oxidative metabolism and nitric oxide production in the trained and untrained female students aged 18-22 years under adaptation to physical load were studied. At the initial stage of the competitive period in the trained students adaptive rearrangements were accompanied by a significant increase in the intensity of the oxidative metabolism (an increase in the rate of generation of the superoxide radical, hydroxyl radical, uric acid content, hydrogen peroxide), production of nitric oxide (increased activity of the constitutive NOS and decrease in the content of the marker of peroxynitrite - nitrate anion) and lipid peroxidation. The growth of the content of TBA-active products indicates the intensification of these processes. High (r≥0.70) correlation dependence among trained girls students between the functional state of the organism and the indicators of the intensity of oxidative metabolism and nitric oxide production was revealed. Our results point for the important role of these processes in the formation of adaptive changes in the body under systematic physical load. [ABSTRACT FROM AUTHOR]