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Additional file 1: Figure S1. (a) The density distribution map of transcript per million (TPM). (b) The bubble chart showing the KEGG pathways of 739 DEGs. (c) Venn diagram illustrating the overlapped KEGG pathways. (d) KEGG pathway annotation of intersection and specific parts in Figure S1c. (The color represented each comparison groups are same with Figure S1c). Figure S2. (a) Power value for the adjacency matrix in WGCNA, where the red line signals 0.85 on the vertical axis. (b) The mean connectivity of WGCNA analysis. Figure S3. (a) Hierarchical cluster tree showing coexpression modules identified by WGCNA. (b) Validation of the transcriptome data by qRT-PCR. (r were calculated by Pearson correlation). Figure S4. (a) The relative ratio of erythroid cells during terminal erythropoiesis stage. (Kruskal-Wallis test was used and P value were corrected by bonferroni) (b) Module-trait relationships plot. Each row corresponds to a module, column to different cell types during terminal erythropoiesis. (c) Heatmap of genes involved in the NF-kappa B signaling pathway. Figure S5. The correlation between erythrocyte differentiation related genes in turquoise module with Hif1a.

The feasibility of Clostridium botulinum toxin type D as a novel biological rodenticide to control plateau zokors was explored. In this study, Horn’s method was used to determine the gavage LD50 of C. botulinum toxin type D to plateau zokors. Modified Karber metho was employed to determine the oral LD50 of granular and wheat baits. Results showed that the gavage LD50 was 5840 MLD/kg (the reliable line is 3430–9950 MLD/kg) and the oral LD50 values of granular and wheat baits were 0.8339 and 1.319 g/kg, respectively, which indicated that plateau zokors are sensitive to this toxin. The palatability of wheat and homemade granular baits to plateau zokors was measured. Selective feeding coefficients were 1.33 and 1.10, whereas nonselective feeding coefficients were 0.81 and 0.60, respectively. A plot experiment of different toxin concentrations to kill zokors showed that homemade granular bait (10000 MLD/g) had the best effect. The average efficiency rate was 89.83%. This study showed the feasibility of controlling plateau zokors by using botulinum toxin type D.