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Additional file 4: Figure S2. Analysis of IAA and oxIAA background concentrations in liquid media containing AtDAO1- or GFP-producing bacteria or no bacteria prior supplementation with exogenous IAA (T0). Mean ± SD (n = 3).

Additional file 2: Figure S1. Western blot analysis of IPTG-induced E. coli harboring recombinant AtDAO1, AtGH3.6, AtGH3.17, AtUGT84B1, AtUGT74D1 or GFP construct. Bacterial cultures were treated with 0.1 mM IPTG and incubated 6 h at 20 °C. For bacterial enzymatic assays, production of recombinant protein was obtained with over-night incubation at 20 °C in the presence of 0.1 mM IPTG. Calculated MW of recombinant proteins: 35.7 kDa (AtDAO1), 69.9 kDa (AtGH3.6 and AtGH3.17), 51.7 kDa (AtUGT84B1), 51.1 kDa (AtUGT74D1) and 27 kDa (GFP).