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Additional file 1: Figure S1. Co-localization and expression of glut1 and eNOS in A549 cells. IF was performed for GLUT1 (SLC2A1) and eNOS as described in the main text. (a) Secondary antibody only control, (b) glut-1 (cyan) and eNOs (magenta) localized largely to the plasma membrane, and (c) Line-scan showing colocalization of glut1 and eNOS. Scale bar is 10 μm. Figure S2. Inhibition of PI3K with 1μg/ml LY294002 reverses SC79-induced p-Akt. A549s were treated with SC79 (10μg/ml; DMSO only as control) or PI3K inhibitor LY294002 (1μg/ml) for 1 h before incubating with SC79 for 2 h. Cell lysates were collected for Western. Bars are means ± SEM; n = 3 experiments. (a) *p