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2 results on '"Amoss, MS"'

1. Pituitary and Ovarian Responses to Gonadotropin Releasing Hormone, Calf Removal and Progestogen in Anestrous Beef Cows2

Author

Amoss Ms, Ellersieck Mr, Lishman Aw, Wiltbank Jn, E. K. Inskeep, Harms Pg, G. S. Lewis, and M. F. Smith

Subjects
medicine.medical_specialty, Endocrinology, Progestogen, Internal medicine, medicine.medical_treatment, Genetics, medicine, Animal Science and Zoology, General Medicine, Gonadotropin-releasing hormone, Biology, Food Science
Published
1983
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2. Synthetic polyamines added to cultures containing bovine sera reversibly inhibit in vitro parameters of immunity

Author

William J. Byrd, Amoss Ms, and Jacobs Dm

Subjects
Lipopolysaccharides, Spermidine, Lymphocyte, Spermine, chemical and pharmacologic phenomena, Lymphocyte Activation, chemistry.chemical_compound, Immune system, Antigen, Lectins, Concanavalin A, medicine, Lymphocytes, Cells, Cultured, Phytohaemagglutinin, Immunity, Cellular, Multidisciplinary, biology, Pokeweed mitogen, Polysaccharides, Bacterial, DNA, Cytotoxicity Tests, Immunologic, Molecular biology, Culture Media, Blood, medicine.anatomical_structure, Biochemistry, chemistry, biology.protein, Lymphocyte Culture Test, Mixed, Spleen
Abstract

POLYAMINES are present in high concentration in foetal1 and neoplastic tissue2 and seminal fluid3—all representing antigenic challenges which usually fail to elicit the appropriate immune response. This has prompted us to investigate the influence of polyamines on in vitro parameters of immunity. We report here that micromolar quantities of spermine and spermidine reversibly inhibit three responses of primary cultures of murine spleen cells. These are (1) DNA synthetic response stimulated by phytohaemagglutinin (PHA), pokeweed mitogen (PWM), concanavalin A (con A) or bacterial lipopolysaccharide (LPS); (2) mixed lymphocyte response (MLC) stimulated by irradiated allogeneic cells; and (3) the induction but not the expression of the cytolytic lymphocyte response. The inhibition does not seem to be due to toxicity but occurs only in the presence of calf or foetal calf sera.

Published
1977
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