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The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial, directional and controllable differentiation of mesenchymal stem cells (MSCs) into cartilage and bone compartments. Emerging organoid technology offers new opportunities for osteochondral regeneration. In this study, we developed gelatin-based microcryogels customized using hyaluronic acid (HA) and hydroxyapatite (HYP), respectively for inducing cartilage and bone regeneration (denoted as CH-Microcryogels and OS-Microcryogels) through in vivo self-assembly into osteochondral organoids. The customized microcryogels showed good cytocompatibility and induced chondrogenic and osteogenic differentiation of MSCs, while also demonstrating the ability to self-assemble into osteochondral organoids with no delamination in the biphasic cartilage-bone structure. Analysis by mRNA-seq showed that CH-Microcryogels promoted chondrogenic differentiation and inhibited inflammation, while OS-Microcryogels facilitated osteogenic differentiation and suppressed the immune response, by regulating specific signaling pathways. Finally, the in vivo engraftment of pre-differentiated customized microcryogels into canine osteochondral defects resulted in the spontaneous assembly of an osteochondral unit, inducing simultaneous regeneration of both articular cartilage and subchondral bone. In conclusion, this novel approach for generating self-assembling osteochondral organoids utilizing tailor-made microcryogels presents a highly promising avenue for advancing the field of tissue engineering.

The independence number of a square matrix $A$, denoted by $\alpha(A)$, is the maximum order of its principal zero submatrices. Let $S_n^{+}$ be the set of $n\times n$ nonnegative symmetric matrices with zero trace. Denote by $J_n$ the $n\times n$ matrix with all entries equal to one. Given any integer $n$, we prove that a linear map $\phi: S_n^+\rightarrow S_n^+$ satisfies $$\alpha(\phi(X))= \alpha(X) {\quad\rm for~ all\quad}X\in S_n^+$$ if and only if there is a permutation matrix $P$ such that $$\phi(X)=H\circ(P^TXP)\quad { \rm for~ all\quad}X\in S_n^+,$$ where $H=\phi(J_n-I_n)$ with all off-diagonal entries positive.

Abstract: Intracerebral hemorrhage (ICH) in preterm infants is one of the major co-morbidities of preterm birth and is associated with long-term neurodevelopmental deficits. There are currently no widely accepted treatments to prevent ICH or therapies for the neurological sequelae. With studies broadening the scope of cell death, the newly defined concept of regulated cell death has enriched our understanding of the underlying mechanisms of secondary brain injury after ICH and has suggested potential interventions in preterm infants. In this review, we will summarize the current evidence for regulated cell death pathways in preterm infants after ICH, including apoptosis, necroptosis, pyroptosis, ferroptosis, autophagy, and PANoptosis as well as several potential intervention strategies that may protect the immature brain from secondary injury after ICH through regulating regulated cell death.

usc This paper introduces a label-free DNA sensor based on the based on double microcavities Fabry-Perot interference and Vernier effect. The sensor can simultaneously monitor temperature to avoid interference with the process of measuring DNA. The biosensor is composed of two open microcavities made of C-type optical fiber. One of the microcavities will is functionalized by the probe DNA (pDNA) to hybridize with complementary DNA (cDNA). And the other microcavity is filled with PDMS to realize temperature sensitivity. At the same time, the two cavities will form a Vernier effect, which is sensitive to both temperature and DNA. It is proved by experiments that the temperature sensitivity and refractive index (RI) sensitivity of the sensor are -2.79 nm/°C and -7122.63 nm/RIU, respectively. The proposed sensor has good specificity. cDNA sequence can bind well to given targets, but hardly bind to non-complementary DNA (non-cDNA) sequences. Moreover, the sensor offers some other has the benefits of high sensitivity, dual-parameter detection, real-time response, and simple fabrication. This is of great significance to solve the problem of temperature sensitivity of biosensors. Refereed/Peer-reviewed

The effects of corruption in urban development and urban affairs management in several south Asian countries are examined through a series of specific, distinctive, and provocative cases for which the data is more readily available. The stories and themes provide a starting point for analyzing corruption as a symptom and factor of underdevelopment, affecting efforts to use and allocate scarce resources for a higher quality of life in cities. It shows how corruption stifles imaginative and creative solutions to urban challenges while increasing future revenue sources. 3Ps has provided a chance for the public section to look at various funding expertise and options from the business sector to prepare the public infrastructure. On the other hand, governments in the source of budget limitations and other competing demands for state sources can’t supply each citizenry’s infrastructure. Besides, the private sector has been considered a better resource manager, and the government should concentrate on policymaking. Where P3s are put to fair use, the advantages are immense. Unfortunately, vulnerable to bribery. This is the case; whatever benefits 3P offers in reducing the urban infrastructure deficit may be eroded due to corruption, which could lead to an increase in construction or facility costs.’ rehabilitation. Secondly, a PPP process marred by corruption could lead to inferior construction substances. One of the fund’s big chunks will be diverted to the public officials’ bribing via the project company. Thirdly, a corrupt process could compromise officials’ integrity that has been charged with accountability for inspecting and approving construction works.