1. Sponge-induced angiogenesis and inflammation in PAF receptor-deficient mice (PAFR-KO)
- Author
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Ferreira, Mônica A N D, Barcelos, Lucíola S, Campos, Paula P, Vasconcelos, Anilton C, Teixeira, Mauro M, and Andrade, Silvia P
- Subjects
Dihydropyridines ,Neutrophils ,Administration, Topical ,Polyurethanes ,Neovascularization, Physiologic ,Platelet Membrane Glycoproteins ,Receptors, G-Protein-Coupled ,Hemoglobins ,Mice ,Implants, Experimental ,Acetylglucosaminidase ,Humans ,Animals ,Platelet Activating Factor ,Peroxidase ,Skin ,Inflammation ,Mice, Knockout ,Mice, Inbred BALB C ,Granuloma ,Neovascularization, Pathologic ,Macrophages ,Imidazoles ,Fibroblasts ,Mice, Mutant Strains ,Porifera ,Papers ,Granulation Tissue ,Commentary ,Blood Vessels ,Chemokines - Abstract
1. To determine biological functions of platelet-activating factor (PAF) in chronic inflammation, we have investigated the kinetics of angiogenesis, inflammatory cells recruitment and cytokine production in sponge-induced granuloma in wild type and PAF receptor-deficient mice (PAFR-KO). 2. Angiogenesis as determined by morphometric analysis and hemoglobin content was significantly higher in the implants of PAFR-KO mice at all time points. Treatment with PAF receptor antagonist UK74505 (30 mg kg(-1)) also increased angiogenesis in sponge implants. 3. Neutrophils and macrophages accumulation, as determined by myeloperoxidase and N-acetylglucosaminidase activities in the supernatant of implanted sponges were markedly decreased in PAFR-KO mice. Surprisingly, the levels of the proinflammatory chemokines, keratinocyte-derived chemokine and chemokine monocyte chemoattractant protein 1 were higher in the implants of the transgenic animals. 4. We have shown that angiogenesis was stimulated in PAFR-KO mice whereas inflammation was decreased, indicating that PAF is an endogenous regulator of new blood vessels formation in the inflammatory microenvironment induced by the sponge implant.
- Published
- 2004