Search

We present a technical note that supplements published procedures on optimal sample preparation for performing wood and phloem formation analyses. Before beginning sampling, it is important to learn about the characteristics of the tree or shrub species to be investigated. Some tips are given how to use the Trephor tool in the best way, how to remove the outer hard bark (periderm), how microcores should be handled after removal from the tree, and how they should be oriented for embedding in paraffin, and cutting thin sections for microscopy. Possible defects that may result from improper handling are illustrated and discussed. We also present optimal images to accurately identify different cell development stages in phloem and xylem, which is particularly challenging in hardwoods and Mediterranean tree and shrub species. Predstavljamo tehnična navodila, ki dopolnjujejo objavljene postopke za optimalno pripravo vzorcev za izvajanje analiz nastajanja lesa in floema. Pred začetkom vzorčenja je potrebno poznati značilnosti drevesne ali grmovne vrste, ki jo želimo preiskovati. Podanih je nekaj nasvetov, kako najbolje uporabiti orodje Trephor, kako odstraniti zunanjo trdo skorjo (periderm), kako ravnati z mikro izvrtki po odvzemu iz debla in kako jih orientirati za vklapljanje v parafin in rezanje tankih rezin za mikroskopske preiskave. Prikazane in razložene so možne napake, ki lahko nastanejo zaradi neustreznega ravnanja v različnih korakih postopka. Predstavljamo tudi optimalne slike tkiv za prepoznavanje različnih razvojnih faz celic v floemu in ksilemu, ki je še posebej zahtevno pri listavcih ter sredozemskih drevesnih in grmovnih vrstah. Bibliografija: str. 56. Izvleček ; Abstract ; Povzetek.

Dendrochronology, the science of dating tree rings in the wood, defines in which calendar year a particular tree ring was formed. The method can be used to determine the age and authentication of wooden musical instruments. We present a protocol describing how to perform a dendrochronological analysis on stringed instruments and how to interpret the dating. The protocol describes the basic steps in the analysis of top plates, which are usually made of Norway spruce (Picea abies) or, more rarely, silver fir (Abies alba). First, the top plate is carefully inspected, and then the tree ring widths are measured directly on the instrument using high-resolution images. After completing the measurements, a tree ring sequence of the instrument is created, and, in the next step, dating is performed with a number of reference chronologies of the tree species from different geographical areas and instruments. The specialists who date the instruments also invest work in creating reference chronologies. The dendrochronological report provides the dating of an instrument as a calendar year (end date), indicating the year in which the last (most recent) tree ring on the top plate was formed when the tree was still alive. The end date represents the terminus post quem, the year after which the instrument was made or before which it could not have been made. To estimate the year of manufacture, one must consider the time required for wood drying and storing and the number of tree rings removed during wood processing. This protocol is intended to help those commissioning such an analysis to better understand how the analysis is performed and how to interpret the dendrochronological reports in terms of the age, origin, maker, and authenticity of the instrument.

Wood in outdoor applications is exposed to abiotic and biotic factors. If we want to slow down the decay, the wood must be protected. In the past, biocidal products based on copper, chromium, and boron compounds (CCB) were one of the most important solutions for wood protection under extreme conditions. Although CCB is in practice no longer used in the EU, it can serve as a reference for the evaluation of new biocidal products. At the field test site of the Department of Wood Science and Technology, Biotechnical Faculty, an experiment has been carried out under real conditions for 14 years, in which impregnated samples are exposed to the weather according to a double-layer test. In the case of treated wood, we often find that the wood decays faster than expected. In this work we want to determine what contributes to decay based on the analysis of decayed impregnated wood from the field test site. The results show that sufficient retention and penetration of the active substances into the wood ensures the planned service life.

Temporal variability of leaf senescence (autumn phenology) was observed in 2020 in 11 European beech (Fagus sylvatica) trees in Tivoli, Rožnik and Šišenski hrib Landscape Park in Ljubljana, Slovenia, and also observed for spring phenology in the same year. General leaf colouring, BBCH94, occurred between 19 and 24 October 2020, with lower inter-individual variability than that of leaf unfolding, BBCH11. The trees had active leaves (time between leaf unfolding and leaf colouring) between 177 and 199 days. In only three trees total leaf fall, BBCH97, occurred before 19 November 2020. Leaf colouring of the tree included in the long-term monitoring program of the Slovenian Environment Agency ARSO occurred on 24 October 2020. This is 7 days later than the 65-year average of the same tree/location and is ascribed to weather conditions. Investigation of tree tissues showed that the width of the last formed tree-ring in the wood varied between 0.39 and 9.61 mm and in the phloem between 0.09 and 0.26 mm, and that the tissues reflect the health condition of the trees.

Wood identification of barbecue charcoal from commercial packages of three retailers (B1, B2, B3) in Slovenia and Croatia was performed with help of Confocal Laser Scanning Microscopy (CLSM). CLSM enabled us to image key identification features of charcoal wood that were compared with light micrographs of wood from the reference collection. Product B1 contained charcoal made exclusively of beech wood (Fagus sylvatica) and the declaration indicated the address of the producer, in Serbia which allowed traceability of the wood. The selection of wood species in product B2, consisted of red oak (Quercus cerris or Q. rubra), black locust (Robinia pseudoacacia), and cherry (Prunus avium), which could originate from Serbia, and it did not contain tropical wood as stated on the package. Product B3 contained wood from at least four (sub)topical species which could not be exactly identified to species/genus level. The declaration on the product did not allow traceability of wood. As the risks of illegal logging are high for wood of (sub)tropical origin, our results support the initiative that the monitoring of the charcoal trade should be covered by the EUTR - European Timber Regulations.

Forests are under great pressure due to climate changes. It is forecast that the importance of Mediterranean type forests will significantly increase. Among various types of oak species, pubescent oak (Quercus pubescens) will likely gain ecological and economic importance in the region south of Alps. Although this wood species is well known, there are not much data available about chemical properties and durability. A comprehensive analysis of extractives and durability against wood decay fungi was therefore performed. The results of the chemical analysis revealed that heartwood contains up to 20% of the extractives, with considerable amounts of phenols, including a fairly high concentration of Gallic acid (GAc). Anatomical structure, with high frequency of tylosis, resulted in good water exclusion efficacy. The presence of biologically active extractives and superior water exclusion efficacy resulted in good durability, as shown by basidiomycetes tests. Based on the laboratory data, factors that determine the service life of wood were calculated. Data indicate superior performance in above-ground applications.

Mediterranean trees and shrubs form intra-annual density fluctuations (IADFs) in tree rings as a sign of their plasticity in wood formation in response to intraseasonal variations of environmental conditions. Different species show a different aptitude to form IADFs, due to their diverse ability to cope with climate stressors, since the occurrence of IADFs may affect plant hydraulics. Dendroecology and quantitative wood anatomy were used to characterise IADFs in Pinus pinea and Arbutus unedo co-occurring at a Mediterranean site in Italy. The relations between climate parameters (i.e. temperature and precipitation) and intra-annual tree-ring traits (i. e. IADF frequency and conduit size) were analysed to highlight the main triggers for IADF formation and their functional role.Data showed that both species are characterised by a high plastic response to climate and formed a high frequency of L-IADFs (occurrence of earlywoodlike conduits in latewood). The two species, although forming the same type of IADFs, showed different sensitivity to environmental factors. Pinus pinea showed a high dependence of tracheid size on temperature, while Arbutus unedo was more sensitive to precipitation in spring and autumn. Arbutus unedo promptly developed more than one IADF per year in response to rainfall events following drought periods.The overall results were useful to compare the aptitude of the two species in forming IADFs and to highlight the factors priming their formation. This is useful to understand wood growth reactions to environmental drivers and to evaluate the adaptive capabilities in these two species, and thus to predict forest reactions after climate changes.

Survival of forest tree species in semi-arid environments such as the Mediterranean area is threatened by the increase in harsh drought conditions. Therefore, better knowledge of the eco-physiology of Mediterranean species and their growth responses to climatic factors is needed to develop strategies for sustainable management.The studies of cambial activity and wood formation can provide information on tree growth and physiological responses to variations in intra-annual climatic parameters, helping to answer questions related to tree performance and plasticity under changing environmental conditions.Our aim was to investigate cambium production in holm oak (Quercus ilex L.) as one of the most widespread evergreen oaks in Southern Italy. We studied the response of cambium activity throughout the particularly dry year 2017. We collected tissues from tree stems every two weeks by sampling microcores containing phloem, cambium and xylem. For the analyses, thin cross sections of the microcores were analysed under a light microscope to identify the timing of cambial production, xylogenesis and phloem formation. We detected the period in which the cambium was active, as well as the period in which the cambium was not productive. We interpreted the cambial response to climatic conditions at the site during the period of observation.The observed pattern of xylogenesis differed from the expected bi-modal pattern typical for Mediterranean species which usually results in Intra-annual Density Fluctuations (IADFs) in tree-rings. In Q. ilex we observed only one peak of cambial activity, likely due to the low water availability during the year 2017.The obtained results provide useful information supporting the forecasting of the wood-growth responses to expected climate change. Moreover, we gained technical experience on optimal preparation of thin sections of problematic tissues, which is especially challenging in Q.ilex due to high hardness of the peculiar wood structure making the investigations of xylogenesis very challenging in this species.

A local tree-ring chronology of sessile oak (Qercus petraea) was constructed for the site Klanec pri Kozini (KLA), Slovenia (45.59° N, 13.92° E, 450 m a.s.l.) located on the Karst edge on the transition from the sub-Mediterranean climatic to temperate Continental zones. The chronology is 93 years long and covers the period 1925–2017. A comparison with four local oak chronologies from Slovenia and 38 from the surrounding countries (distance 65–220 km) showed that KLA agreed best with the local oak chronology of Rožnik, Ljubljana (ROZ), and two other oak chronologies from central Slovenia (the surroundings of Novo mesto and Ljubljana), one from Croatia and one from Austria. Dendroclimatological analysis showed that the main factor affecting tree-ring variation is June temperature (negative effect) and March precipitation (positive effect), which to a great extent explain the relation to other chronologies. The negative effect of June (maximal) temperature has become increasingly significant in the last few decades, which can be ascribed to warming related to ongoing climatic change.

BACKGROUND AND AIMS: Mediterranean trees have patterns of cambial activity with one or more pauses per year, leading to intra-annual density fluctuations (IADFs) in tree rings. We analysed xylogenesis (January 2015–January 2016) in Pinus pinea L. and Arbutus unedo L., co-occurring at a site on Mt. Vesuvius (southern Italy), to identify the cambial productivity and timing of IADF formation. METHODS: Dendrochronological methods and quantitative wood anatomy were applied and enabled IADF identification and classification. KEY RESULTS: We showed that cambium in P. pinea was productive throughout the calendar year. From January to March 2015, post-cambial (enlarging) earlywood-like tracheids were observed, which were similar to transition tracheids. The beginning of the tree ring was therefore not marked by a sharp boundary between latewood of the previous year and the new xylem produced. True earlywood tracheids were formed in April. L-IADFs were formed in autumn, with earlywood-like cells in latewood. In A. unedo, a double pause in cell production was observed, in summer and winter, leading to L-IADFs in autumn as well. Moreover, the formation of more than one IADF was observed in A. unedo. CONCLUSIONS: Despite having completely different wood formation models and different life strategies, the production of earlywood, latewood and IADF cells was strongly controlled by climatic factors in the two species. Such cambial production patterns need to be taken into account in dendroecological studies to interpret climatic signals in wood from Mediterranean trees.

The analysis of the growth behaviour, wood anatomical, and eco-physiological traits of two Bougainvillea genotypes, trained to two shapes and subjected to different irrigation regimes, fully supports the idea that structural properties interact with cultural practices (i.e., training) in determining the adaptive capability of plants, hence their productivity and survival. Bougainvillea species are cultivated for landscaping in the arid Mediterranean region. In Bougainvillea spp., secondary xylem and phloem are formed by successive cambia leading to a peculiar stem anatomy which could favour water storage and plant adaptation under drought conditions. To achieve sustainable production of Bougainvillea ornamental shrubs, it is crucial to understand how different genotypes respond to deficit irrigation and how cultural practices, such as canopy training, may interact with morpho-anatomical traits in modifying the plants’ ability to withstand water deficit. A greenhouse experiment was conducted to determine the effects of deficit irrigation on plant growth, ecophysiology, and branch anatomy in two Bougainvillea genotypes [B. × buttiana ‘Rosenka’, B. ‘Lindleyana’ (=B. ‘Aurantiaca’)] trained to globe and pyramid shapes. Irrigation treatments were based on daily evapotranspiration (ET): control (C, 100 % ET) or deficit irrigation (DI, 25 % ET). The two genotypes exhibited morphological adaptations to cope with water deficit, including reductions in dry weight, leaf number, and lamina size. In both genotypes, the DI-induced increase in stomatal resistance was accompanied by a decrease in stomata size. Water deficit triggered adjustments in wood anatomical functional traits, also depending on canopy shape and genotype, favouring either water conduction efficiency or safety against embolism. The occurrence of a safer hydraulic system in the pyramid-trained plants suggests a better control of water transport, thus supporting better growth performance under DI conditions compared to globe-trained plants. Such differences, induced by different canopy-shape trainings, should be considered in the management of DI.

Vessel occlusion through tyloses or gums is a natural phenomenon occurring with aging and heartwood formation, and in sapwood in response to vessel embolism. These types of vessel occlusion play a crucial role to limit the spread of pathogens and wood decay organisms, also as part of compartmentalization after wounding. In the sapwood, they can be considered to be an effective stress response.Here we review the literature on tyloses and gums in hardwoods, starting with the detailed 19th century account on tyloses by Hermine von Reichenbach. The structural diversity of tyloses (from thin-walled to sclerotic) and gum deposits is highlighted and illustrated. Our understanding of the development of vessel occlusions through vessel contact cells of the ray and axial parenchyma has greatly increased over the last decades, also thanks to ultrastructural and immunocytological studies. We critically discuss the postulated relationships between vessel-to-ray parenchyma pit size and vessel size and the incidence of either tyloses or gums and review the occurrence of these types of vessel occlusions in extant and fossil dicots. All factors identified in the literature as stimuli for vessel occlusion probably act through vessel embolism as a single direct trigger. Attempts in the literature to relate vessel occlusion with mechanisms of vessel refilling and embolism repair are controversial and invite more experimental research.

Buildings on piles have been constructed in Ljubljana since the Bronze Age. The piles were made of different types of wood. In the present study, piles that were erected about 125 years ago were investigated. Investors tend to renovate a building, therefore, the piles were analysed to assess the structural condition of the building. The building showed no signs of damage. To gain access to the piles, a 2 m thick layer of soil was removed. On-site, the following analyses were carried out: drilling resistance with a resistograph and a screw withdrawal test. Part of the piles was isolated and light microscopy, scanning electron microscopy, infrared spectroscopy, dynamic vapour sorption, density analysis, and chemical analysis were performed. Microscopic analysis revealed that the piles were made from the wood of Scots pine (Pinus sylvestris). The results indicate that the wood was severely degraded, mainly by soft-rot fungi and bacteria, resulting in a significant deterioration of its mechanical properties.

Mediterranean tree rings are characterized by Intra-Annual Density Fluctuations (IADFs) due to partly climate-driven cambial activity. IADFs are used as structural signals to gain information on relations between environmental conditions and eco-physiological processes during xylogenesis, with intra-annual resolution. To reach an unbiased synchronization of the IADF position within tree rings and seasonal fluctuations in environmental conditions, it is necessary to know the timing of cambial activity and wood formation, which are species- and site-specific processes. We applied the microcoring technique to analyze xylogenesis in Pinus halepensis and Arbutus unedo. To the best of our knowledge, this is the first attempt to study xylogenesis in a hardwood species forming frequent IADFs. Both species co-occur at a site in southern Italy characterized by a Mediterranean climate. To facilitate tree-ring dating and identification of IADFs, we performed traditional dendroecological analysis. We analyzed xylogenesis during summer, which is considered a constraint for xylogenesis and a trigger for IADF formation. We followed the different phases of cell development in the current wood increment with the aim of evaluating whether and which type of IADFs were formed. We additionally analyzed the same phases again in September and in winter to verify the possible formation of IADFs in fall and whether cell production and differentiation was completed by the end of the calendar year. Both species formed the same type of IADFs (earlywood-like cells within latewood), due to temporary growth restoration triggered by rain events during the period of summer drought. At the end of the calendar year, no cells in the phases of enlargement and secondary cell wall deposition occurred. A. unedo was more sensitive than P. halepensis because IADFs were formed earlier in the season and were more frequent in the tree-ring series. The dendro-anatomical approach, combining analysis of tree-ring series and of xylogenesis, helped to detect the period of IADF formation in the two species. Results are discussed in functional terms, highlighting the environmental conditions triggering IADFs, and also in methodological terms, evaluating the applicability of xylogenesis analysis in Mediterranean woods, especially when the formation of IADFs is not uniform around the stem.