1. Effect of Influenza Vaccination Inducing Antibody Mediated Rejection in Solid Organ Transplant Recipients
- Author
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Elisa Cordero, Angel Bulnes-Ramos, Manuela Aguilar-Guisado, Francisca González Escribano, Israel Olivas, Julián Torre-Cisneros, Joan Gavaldá, Teresa Aydillo, Asunción Moreno, Miguel Montejo, María Carmen Fariñas, Jordi Carratalá, Patricia Muñoz, Marino Blanes, Jesús Fortún, Alejandro Suárez-Benjumea, Francisco López-Medrano, Cristina Roca, Rosario Lara, Pilar Pérez-Romero, Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Regional Development Fund (ERDF/FEDER), Red Española de Investigación en Patología Infecciosa, Universidad de Cantabria, Junta de Andalucía, European Commission, and Instituto de Salud Carlos III - ISCIII
- Subjects
Graft Rejection ,Male ,0301 basic medicine ,Time Factors ,alloreactivity ,0302 clinical medicine ,Cytomegaloviruses ,HLA Antigens ,Isoantibodies ,Risk Factors ,Immunology and Allergy ,Medicine ,Longitudinal Studies ,Prospective Studies ,Original Research ,Histocompatibility Testing ,Vaccination ,Middle Aged ,Flow Cytometry ,Treatment Outcome ,Influenza Vaccines ,Histocompatibility ,Antibody mediated rejection ,Female ,donor specific antibodies ,lcsh:Immunologic diseases. Allergy ,Immunology ,Congenital cytomegalovirus infection ,Influenzavirus ,Risk Assessment ,anti-human leukocyte antigen ,03 medical and health sciences ,Vacunes antivíriques ,Influenza viruses ,Humans ,cytomegalovirus ,Viral vaccines ,business.industry ,Donor specific antibodies ,organ rejection ,Organ Transplantation ,medicine.disease ,Virology ,030104 developmental biology ,Spain ,Citomegalovirus ,lcsh:RC581-607 ,Solid organ transplantation ,business ,Biomarkers ,030215 immunology - Abstract
[Introduction] Our goal was to study whether influenza vaccination induced antibody mediated rejection in a large cohort of solid organ transplant recipients (SOTR)., [Methods] Serum anti-Human Leukocyte Antigen (HLA) antibodies were determined using class I and class II antibody-coated latex beads (FlowPRATM Screening Test) by flow cytometry. Anti-HLA antibody specificity was determined using the single-antigen bead flow cytometry (SAFC) assay and assignation of donor specific antibodies (DSA) was performed by virtual-crossmatch., [Results] We studied a cohort of 490 SOTR that received an influenza vaccination from 2009 to 2013: 110 (22.4%) received the pandemic adjuvanted vaccine, 59 (12%) within the first 6 months post-transplantation, 185 (37.7%) more than 6 months after transplantation and 136 (27.7%) received two vaccination doses. Overall, no differences of anti-HLA antibodies were found after immunization in patients that received the adjuvanted vaccine, within the first 6 months post-transplantation, or based on the type of organ transplanted. However, the second immunization dose increased the percentage of patients positive for anti-HLA class I significantly compared with patients with one dose (14.6% vs. 3.8%; P = 0.003). Patients with pre-existing antibodies before vaccination (15.7% for anti-HLA class I and 15.9% for class II) did not increase reactivity after immunization. A group of 75 (14.4%) patients developed de novo anti-HLA antibodies, however, only 5 (1.02%) of them were DSA, and none experienced allograft rejection. Only two (0.4%) patients were diagnosed with graft rejection with favorable outcomes and neither of them developed DSA., [Conclusion] Our results suggest that influenza vaccination is not associated with graft rejection in this cohort of SOTR., This study was funded by the Consejería de Salud (Grant Number: PI-0119-2012), Ministerio de Economía y Competitividad, Instituto de Salud Carlos III (Grant Numbers: GR09/0041, PI14-00165, and MPY110/18) and co-financed by European Development Regional Fund “A way to achieve Europe” ERDF, Spanish Network for the Research in Infectious Diseases (REIPI RD12/0015/0001).
- Published
- 2020