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Background Neurological damage from spinal cord injury (SCI) is a result of primary mechanical injury and secondary damage from oxidative stress and neuroinflammation. Although genistein has been shown to have potent antioxidant and anti-inflammatory effects in studies of brain injury, its effect on secondary damage in SCI has remained unknown. Objective To determine effects of genistein in a model of SCI in rats. Methods We divided 21 rats evenly into 3 groups, a control group, in which only a laminectomy was performed; a trauma group in which SCI was induced; and a genistein group in which genistein was administered subcutaneously after SCI. The rats were assessed using a Basso–Beattie and Bresnahan functional score at the 12th hour and on the 1st, 3rd, 5th, and 7th days. Biochemical analyses were conducted at the same time points to determine the serum levels of catalase, ischemia-modified albumin (IMA), disulfide (SS), total thiol (TT), native thiol (NT), disulfide/total thiol (SS/TT), and native thiol/total thiol (NT/TT). Total oxidant and antioxidant capacity, and oxidative stress index were determined in spinal cord tissue obtained on the 7th day together with immunohistochemistry for cyclooxygenase-2 levels. Result Catalase activity on the 7th day was significantly (P = 0.001) higher in the genistein-treated rats than in other groups, and IMA levels became stable earlier (3rd day) in the genistein group. SS values were significantly (P = 0.004) lower in the genistein group. NT/TT ratio were significantly (P = 0.049) higher in the genistein-treated rats on the 7th day. Conclusion Genistein has antioxidant, anti-inflammatory, and protective effects in a model of SCI in rats and warrants further study.

Amac: Deneysel omurilik yaralanmalarinda hasarin takibi ve hucrelerin hasara yanitini degerlendirmek ana unsurlardan biridir. Bu calismada iki farkli deneysel omurilik travma modelinde serum ve dokuda oksidan-antioksidan maddelerin degisimi incelenmistir. Gerec ve yontem: Calismada 3 grupta 6 adet Wistar rat kullanildi. Kontrol grubuna (A grubu) sadece laminektomi yapildi. Agirlik grubuna (B grubu) laminektomi ve agirlik dusurme modeli ile, klip-kompresyon grubuna (C grubu) laminektomi ve klip kompresyon teknigi ile spinal kord hasari olusturuldu. Fonksiyonel olarak 12. saat, 1., 3. 5. 7. Gun BBB skorlari ile degerlendirildi. Biokimyasal olarak 12. saatde, 1., 5. ve 7. gun alinan kuyruk kanlarinda oksidan-antioksidan maddeler incelendi. 7.gun sakrifiye edilen deneklerin omurilik dokularinda Total antioksidan kapasite (TAS), Total oksidan kapasite (TOS) ve OSI (oksidatif stres indeksi) bakildi. Bulgular A grubuna gore B ve C grubunda Disulfid(SS)/Total tiol(TT), SS/Native tiol(NT) ve NT/TT oranlari anlamli olarak farkli bulunmustur. Katalaz icin en erken anlamli fark 5. gun ortaya cikmistir. IMA (iskemi modifiye albumin) erken evrede artsa da hasarin 3. gununden itibaren tekrar azalip 7. gun normale yakin degerlere gelmistir. C ile B grubu kiyaslandiginda parametrelerin C grubunda daha anlamli degisiklik gosterdigi gorulmustur. Sonuc Travma modellerinde oksidatif-antioksidatif markerlar hasarin siddetini gostermede ve takibinde kullanilabilir. Klip kompresyon yontemi, serumda oksidatif stres parametrelerini olcmek icin daha basarili bir yontemdir.