1. Identification of 30-kDa heat shock protein gene in Trichophyton rubrum
- Author
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Fatma Sirmatel, Payam Behzadi, Elham Behzadi, BAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Behzadi, Elham, and Sırmatel, Fatma
- Subjects
HSP30 Heat-Shock Proteins ,Molecular Sequence Data ,Dermatology ,Trichophyton rubrum ,Heat Shock Proteins ,Trhsp30 ,Microbiology ,Fungal Proteins ,chemistry.chemical_compound ,Trichophyton ,Heat shock protein ,Dermatophytosis ,Dermatomycoses ,Humans ,Amino Acid Sequence ,Heat shock ,Gene ,biology ,Base Sequence ,General Medicine ,biology.organism_classification ,Molecular biology ,Molecular Weight ,Open reading frame ,genomic DNA ,Trichophyton Rubrum ,Infectious Diseases ,PCR ,chemistry ,GenBank ,Sequence Alignment ,DNA - Abstract
WOS:000264820200005 PubMed: 18643918 Small heat shock proteins (sHSPs) are chaperones that are crucial in the heat shock response but also have important non-stress roles within the cell. HSP70 in Trichophyton rubrum is already detected and carefully characterised; however, no study was carried out for HSP30 in this pathogenic fungus. In the present study, T. rubrum was obtained from patients with dermatophytosis and cultured in appropriate conditions. High-molecular-weight DNA was extracted using standard extraction methods. Pairs of 21 nt primers were designed from highly conserved regions of the similar genes in other eukaryotic cells. Mentioned primers were utilised in PCR using isolated genomic DNA and extracted RNA templates of T. rubrum. The PCR fragments were then sequenced and 415 nucleotides of HSP30 in this pathogenic fungus were detected; the open reading frame had 156 nucleotides and was coding 51 amino acids. This gene (called TrHSP30) is registered in GenBank at National Center for Biotechnology Information (NIH, USA) database. Detection of TrHSP30 gene may open the way to determination of its possible role in the pathogenesis of dermatophyte infections due to T. rubrum.
- Published
- 2008