1. An immune-based tool platform for in vivo cell clearance
- Author
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Zhang, Jieqiong, Tsukui, Tatsuya, Wu, Xiumin, Brito, Alyssa, Trumble, John Maxwell, Caraballo, Juan C, Allen, Greg M, Zavala-Solorio, José, Zhang, Chunlian, Paw, Jonathan, Lim, Wendell A, Geng, Jiefei, Kutskova, Yuliya, Freund, Adam, Kolumam, Ganesh, Sheppard, Dean, and Cohen, Robert L
- Subjects
Kinetics ,Underpinning research ,Pulmonary Fibrosis ,1.1 Normal biological development and functioning ,Inflammatory and immune system ,Hepatocytes ,Humans ,Fibroblasts ,Antibodies - Abstract
Immunological targeting of pathological cells has been successful in oncology and is expanding to other pathobiological contexts. Here, we present a flexible platform that allows labeling cells of interest with the surface-expressed model antigen ovalbumin (OVA), which can be eliminated via either antigen-specific T cells or newly developed OVA antibodies. We demonstrate that hepatocytes can be effectively targeted by either modality. In contrast, pro-fibrotic fibroblasts associated with pulmonary fibrosis are only eliminated by T cells in initial experiments, which reduced collagen deposition in a fibrosis model. This new experimental platform will facilitate development of immune-based approaches to clear potential pathological cell types in vivo.
- Published
- 2023