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Supplementary filefor "A Next Generation Sequencing-based Protocol for Screening of Variants of Concern in Autism Spectrum Disorder." The supplementary file included corresponds to Figure S1: Average sequencing depth (bar plot) and coverage (dot-line plot) in each chromosome. The x-axis represents chromosome; the left y-axis is the average depth; the right y-axis is the cov-erage (proportion of covered bases).; Figure S2: Sample ASD WGS genetics report for participants.; Table S1: Sanger sequencing primer information.