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1. Design and Modeling of a Flexible Conductive Fabric Antenna Integrated in an OLED Light Source for WIMAX Wireless Communication Systems

Author

Mustapha El Halaoui, Laurent Canale, Soukaina Sekkal, Othmane Bendaou, Adel Asselman, Optics and Photonics Laboratory, Université Abdelmalek Essaadi, Tetouan, Morocco, Faculté des Sciences de Tétouan, LAboratoire PLasma et Conversion d'Energie (LAPLACE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Lumière et Matière (LAPLACE-LM), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Centre National de la Recherche Scientifique (CNRS), Modeling and Simulation of Mechanical Systems Laboratory, Université Abdelmalek Essaâdi (UAE), This work is supported by a grant from the PHC Maghreb (Hubert Curien Program) MELINA (Mastering Efficient Lighting in North Africa). Funding is provided by Campus France (Grant number 43981ZG awarded to L.C.) and the French Ministries of Europe and Foreign Affairs, and in Morocco, by the Ministry of Higher Education and Scientific Research, in partnership with the National Center for Scientific and Technical Research (CNRST)., and PHC Maghreb (Hubert Curien Program) MELINA (Mastering Efficient Lighting in North Africa). Campus France (Grant number 43981ZG)

Subjects
Materials science, Wireless Communication Systems, 02 engineering and technology, Flexible Antenna, 7. Clean energy, Radiation pattern, 0202 electrical engineering, electronic engineering, information engineering, OLED, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Reflection coefficient, Electrical conductor, Organic Light-Emitting Diode, business.industry, [SPI.NRJ]Engineering Sciences [physics]/Electric power, 020206 networking & telecommunications, 021001 nanoscience & nanotechnology, WiMAX, [SPI.TRON]Engineering Sciences [physics]/Electronics, Transparent Conductive Fabric, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Optoelectronics, Antenna (radio), 0210 nano-technology, business, Layer (electronics), Microwave
Abstract

International audience; This work presents a new bendable antenna for worldwide interoperability for microwave access (WiMAX) wireless communication systems. These antennas, transparent and flexible, will be easily integrated into various mdia and in particular OLED lighting which could be part of the public lighting network of tomorrow as well as on all display media. The integration of these antennas as close as possible to the end-user is a possible solution to reduce the energy consumption which goes hand in hand with the increase in the data rate. This kind of new antenna, designed to be integrated in organic light-emitting diode (OLED), was modeled from a transparent VeilShieldTM conductive fabric and was placed on a 100% polyester substrate with a thickness of 1.5 mm and a loss tangent of 0.02. We have tested and evaluated the characteristic parameters of our antenna, namely the reflection coefficient, the radiation pattern and the gain, to find out the performance of our proposed design. The performance of the transparent conductive fabric integrated in the 100% polyester substrate is tested for the application of flexible antenna operating at 3.5 GHz with a gain value of 5.38 dB. We have integrated this proposed new antenna with the OLED light source containing four layers of different materials and electrical properties: aluminum cathode layer, polymer layer, indium tin oxide (ITO) anode layer and glass substrate layer. After integration, the resonant frequency shifted to 3.52 GHz with a gain value of 4.61 dB. In addition, we also tested the concave bending on the reflection coefficient of the proposed flexible antenna taking into account the different bending angles. This work demonstrates the possibility of integrating these unconventional materials used for the proposed antenna within the OLED despite weak effects on the resonant frequency and the gain of the proposed antenna after integration.

Published
2021

2. Occupational exposure to disinfectants and asthma incidence in U.S. nurses: A prospective cohort study

Author

Carlos A. Camargo, Frank E. Speizer, Paul K. Henneberger, Raphaëlle Varraso, Nicole Le Moual, Catherine Quinot, Orianne Dumas, Jan-Paul Zock, Krislyn M. Boggs, Vieillissement et Maladies chroniques : approches épidémiologique et de santé publique (VIMA), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), PCOFUND-GA-2013-609102 National Institutes of Health, NIH Centers for Disease Control and Prevention, CDC: UM1 CA‐176726 Marie Curie Research Executive Agency, REA Université Pierre et Marie Curie, UPMC, The Nurses? Health Study II is coordinated at the Channing Division of Network Medicine, Brigham and Women's Hospital, Boston, MA. We would like to thank the participants and staff of the Nurses? Health Study II for their valuable contributions. Support for this study was provided by grants R01 OH-010359 (Centers for Disease Control and Prevention), UM1 CA-176726 (National Institutes of Health) and funding from the People Program (Marie Curie Actions) of the European Union's Seventh Framework Program (FP7/2007-2013) under REA grant agreement no. PCOFUND-GA-2013-609102, through the PRESTIGE program coordinated by Campus France. John Meyer declares that he has no conflict of interest in the review and publication decision regarding this article., and The Nurses’ Health Study II is coordinated at the Channing Division of Network Medicine, Brigham and Women's Hospital, Boston, MA. We would like to thank the participants and staff of the Nurses’ Health Study II for their valuable contributions. Support for this study was provided by grants R01 OH‐010359 (Centers for Disease Control and Prevention), UM1 CA‐176726 (National Institutes of Health) and funding from the People Program (Marie Curie Actions) of the European Union's Seventh Framework Program (FP7/2007‐2013) under REA grant agreement no. PCOFUND‐GA‐2013‐609102, through the PRESTIGE program coordinated by Campus France.

Subjects
Adult, Disinfectant, Population, Nurses, Article, 03 medical and health sciences, 0302 clinical medicine, Occupational Exposure, Surveys and Questionnaires, Environmental health, medicine, Humans, Prospective Studies, 030212 general & internal medicine, Asthma, Occupational, Prospective cohort study, education, disinfection, Asthma, 2. Zero hunger, education.field_of_study, healthcare workers, business.industry, Incidence, Hazard ratio, Public Health, Environmental and Occupational Health, Middle Aged, asthma, medicine.disease, United States, Confidence interval, 3. Good health, 030228 respiratory system, Female, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, cleaning products, occupational asthma, business, Occupational asthma, Body mass index, Disinfectants
Abstract

International audience; Background: Exposure to disinfectants among healthcare workers has been associated with respiratory health effects, in particular, asthma. However, most studies are cross-sectional and the role of disinfectant exposures in asthma development requires longitudinal studies. We investigated the association between occupational exposure to disinfectants and incident asthma in a large cohort of U.S. female nurses. Methods: The Nurses’ Health Study II is a prospective cohort of 116 429 female nurses enrolled in 1989. Analyses included 61 539 participants who were still in a nursing job and with no history of asthma in 2009 (baseline; mean age: 55 years). During 277 744 person-years of follow-up (2009-2015), 370 nurses reported incident physician-diagnosed asthma. Occupational exposure was evaluated by questionnaire and a Job-Task-Exposure Matrix (JTEM). We examined the association between disinfectant exposure and subsequent asthma development, adjusted for age, race, ethnicity, smoking status, and body mass index. Results: Weekly use of disinfectants to clean surfaces only (23% exposed) or to clean medical instruments (19% exposed) was not associated with incident asthma (adjusted hazard ratio [95% confidence interval] for surfaces, 1.12 [0.87-1.43]; for instruments, 1.13 [0.87-1.48]). No association was observed between high-level exposure to specific disinfectants/cleaning products evaluated by the JTEM (formaldehyde, glutaraldehyde, bleach, hydrogen peroxide, alcohol quats, or enzymatic cleaners) and asthma incidence. Conclusions: In a population of late career nurses, we observed no significant association between exposure to disinfectants and asthma incidence. A potential role of disinfectant exposures in asthma development warrants further study among healthcare workers at earlier career stage to limit the healthy worker effect.

Published
2019

3. Rapid and Label-Free Electrochemical DNA Biosensor for Detecting Hepatitis A Virus

Author

Sara Viezzi, Sandra Mazerat, Marisa Manzano, Robert S. Marks, Jasmina Vidic, Università degli Studi di Udine - University of Udine [Italie], Nanyang Technological University (NTU), Université Paris Saclay (COmUE), Ben-Gurion University of the Negev (BGU), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Campus France through the BioAsia Grant [35976PH], and Campus France through Galilee Grant [37184VG]

Subjects
0209 industrial biotechnology, [SDV]Life Sciences [q-bio], viruses, Biosensing Techniques, 02 engineering and technology, Tripropylamine oxidation, 01 natural sciences, law.invention, 020901 industrial engineering & automation, Limit of Detection, law, Electrochemistry, Polymerase chain reaction, Chemistry, Hybridization probe, DNA–DNA hybridization, Nucleic Acid Hybridization, virus diseases, General Medicine, Hepatitis A, 021001 nanoscience & nanotechnology, 3. Good health, Hepatitis A virus detection, Cyclic voltammetry, DNA-electrochemical biosensor, AFM, DNA Probes, 0210 nano-technology, Biotechnology, Biophysics, Biomedical Engineering, DNA, Single-Stranded, lcsh:A, Nucleic acid thermodynamics, On-electrode hybridization, Complementary DNA, Humans, Detection limit, Base Sequence, 010401 analytical chemistry, Electrochemical Techniques, Molecular biology, digestive system diseases, 0104 chemical sciences, n/a, Food, DNA, Viral, Hepatitis A virus, lcsh:General Works, Biosensor
Abstract

International audience; Diagnostic systems that can deliver highly specific and sensitive detection of hepatitis A virus (HAV) in food and water are of particular interest in many fields including food safety, biosecurity and control of outbreaks. Our aim was the development of an electrochemical method based on DNA hybridization to detect HAV. A ssDNA probe specific for HAV (capture probe) was designed and tested on DNAs from various viral and bacterial samples using Nested-Reverse Transcription Polymerase Chain Reaction (nRT-PCR). To develop the electrochemical device, a disposable gold electrode was functionalized with the specific capture probe and tested on complementary ssDNA and on HAV cDNA. The DNA hybridization on the electrode was measured through the monitoring of the oxidative peak potential of the indicator tripropylamine by cyclic voltammetry. To prevent non-specific binding the gold surface was treated with 3% BSA before detection. High resolution atomic force microscopy (AFM) confirmed the efficiency of electrode functionalization and on-electrode hybridization. The proposed device showed a limit of detection of 0.65 pM for the complementary ssDNA and 6.94 fg/μL, for viral cDNA. For a comparison, nRT-PCR quantified the target HAV cDNA with a limit of detection of 6.4 fg/μL. The DNA-sensor developed can be adapted to a portable format to be adopted as an easy-to-use and low cost method for screening HAV in contaminated food and water. In addition, it can be useful for rapid control of HAV infections as it takes only a few minutes to provide the results.

Published
2017

4. Low Temperature Synthesis and Phosphorescence of Methylcyanotriacetylene

Author

Claudine Crépin, Urszula Szczepaniak, Robert Kołos, Jean-Claude Guillemin, Marcin Gronowski, Institut des Sciences Moléculaires d'Orsay (ISMO), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institute of Physical Chemistry, Polska Akademia Nauk = Polish Academy of Sciences (PAN), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Centre National d’Etudes Spatiales, Campus France, 2011/03/B/ST4/02763, Narodowe Centrum Nauki, Conseil National de la Recherche Scientifique, European Social Fund, 27752QB, Hubert Curien Partnership, Campus France, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Rare gas, [CHIM.ORGA]Chemical Sciences/Organic chemistry, 010402 general chemistry, Propyne, 01 natural sciences, Electron spectroscopy, 0104 chemical sciences, Characterization (materials science), [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, chemistry.chemical_compound, chemistry, 0103 physical sciences, Physical chemistry, Density functional theory, Physical and Theoretical Chemistry, Phosphorescence, 010303 astronomy & astrophysics
Abstract

International audience; This paper reports on UV-stimulated synthesis of methylcyanotriacetylene carried out in cryogenic rare gas matrixes via coupling of smaller precursors: propyne and cyanodiacetylene. The detection was possible due to the strong visible ã 3A' → X̃ 1A1 phosphorescence of CH3C7N, discovered in the course of this work. The ensuing measurements of electronic spectroscopy revealed the formally forbidden B̃ 1E-X̃ 1A1 system, as well as the allowed one Ẽ 1A1-X̃ 1A1, with origins at approximately 3.32 and 5.4 eV, respectively. It was also possible to revisit the spectroscopic characterization of cyanotriacetylene, HC7N, formed in parallel to the title photoproduct. Spectral assignments were assisted with a density functional theory study.

Published
2017

5. Aging effect on microcirculation: A multiscale entropy approach on laser speckle contrast images

Author

Khalil, Adil, Humeau-Heurtier, Anne, Gascoin, L., Ábrahám, Pierre, Mahé, Guillaume, allard, christelle, Laboratoire Angevin de Recherche en Ingénierie des Systèmes (LARIS), Université d'Angers (UA), Biologie Neurovasculaire et Mitochondriale Intégrée (BNMI), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Investigation Clinique [Rennes] (CIC), Université de Rennes (UR)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), We gratefully acknowledge the support provided by the ministry of higher education and scientific research in Iraq (Baghdad), the Embassy of France in Iraq (service of cooperation and cultural action in Baghdad), and Campus France Paris, France., Embassy of France in Iraq (service of cooperation and cultural action in Baghdad), Campus France Paris, France, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Hôpital Pontchaillou-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Nantes Angers Le Mans, Biologie Neurovasculaire et Mitochondriale Intégrée, Centre National de la Recherche Scientifique (CNRS) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université d'Angers (UA), Université de Rennes 1 (UR1) - Hôpital Pontchaillou - Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Angers (UA)

Subjects
alzheimers-disease patients, Adult, skin, Aging, Entropy, sample entropy, Young Adult, endothelial function, Humans, microvascular blood-flow, ComputingMilieux_MISCELLANEOUS, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing, Aged, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Lasers, Microcirculation, age-related-changes, Optical Imaging, Equipment Design, Middle Aged, Forearm, Regional Blood Flow, perfusion signal, chaos theory, heart-rate-variability, complexity, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, Algorithms
Abstract

International audience; Purpose: It has long been known that age plays a crucial role in the deterioration of microvessels. The assessment of such deteriorations can be achieved by monitoring microvascular blood flow. Laser speckle contrast imaging (LSCI) is a powerful optical imaging tool that provides two-dimensional information on microvascular blood flow. The technique has recently been commercialized, and hence, few works discuss the postacquisition processing of laser speckle contrast images recorded in vivo. By applying entropy-based complexity measures to LSCI time series, we present herein the first attempt to study the effect of aging on microcirculation by measuring the complexity of microvascular signals over multiple time scales. Methods: Forearm skin microvascular blood flow was studied with LSCI in 18 healthy subjects. The subjects were subdivided into two age groups: younger (20-30 years old, n = 9) and older (50-68 years old, n = 9). To estimate age-dependent changes in microvascular blood flow, we applied three entropy-based complexity algorithms to LSCI time series. Results: The application of entropy-based complexity algorithms to LSCI time series can differentiate younger from older groups: the data fluctuations in the younger group have a significantly higher complexity than those obtained from the older group. Conclusions: The effect of aging on microcirculation can be estimated by using entropy-based complexity algorithms to LSCI time series. (C) 2016 American Association of Physicists in Medicine.

Published
2016

6. The cradle of giants: insights into the origin of Scherotheca Bouché, 1972 (Lumbricidae, Crassiclitellata) with the descriptions of eight new species from Corsica, France

Author

Marchán, Daniel Fernández, Domínguez, Jorge, Hedde, Mickael, Decaëns, Thibaud, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Universidade de Vigo, Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), naturalist expedition, 'Our Planet Reviewed -Corsica 2019-2021'. This survey was organized by the Museum national d'Histoire naturelle (MNHN, Paris) in collaboration with and funded by the Collectivite de Corse and the Office francais de la Biodiversite (OFB)., Make Our Planet Great Again Postdoctoral grant from Campus France (mopga-postdoc-3-6111272103), and Xunta de Galicia (Conselleria de Cultura, Educacion e Ordenacion Universitaria. Secretaria Xeral de Universidades under grant ED431B 2019/038).

Subjects
new species, new combination, Annelida, Corsica, Biodiversity, Eumenescolex, Scherotheca, Crassiclitellata, Animalia, Clitellata, Animal Science and Zoology, Oligochaeta, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, molecular phylogeny, Lumbricidae, Ecology, Evolution, Behavior and Systematics, Taxonomy
Abstract

Copyright BOLD © 2014-2022; International audience; Scherotheca Bouché, 1972 is a highly diverse genus of Lumbricidae Rafinesque-Schmaltz, 1815, broadly distributed from Northern Italy and Corsica Island to the Pyrenees and Northeastern Iberian Peninsula. A recent survey of earthworm species diversity in Corsica resulted in the identification of 12 operational taxonomic units (OTU) within Scherotheca and the closely related genus Eumenescolex Qiu & Bouché, 1998, based on both morphological characters and COI barcodes. In this work, we complete molecular analyses to provide a dataset including four DNA markers (COI, 16S, 28S and ND1) and analyze it through Bayesian phylogeny reconstruction and barcode gap analysis. Our results highlight the existence of eight phylogenetically and morphologically distinct species, new for science, which we described herein as Scherotheca altarocca Marchán, Decäens & Domínguez, n. sp., Scherotheca marceli Marchán, Decäens & Domínguez, n. sp., Scherotheca darioi Marchán, Decäens & Domínguez, n. sp., Scherotheca boccaverghju Marchán, Decäens & Domínguez, n. sp., Scherotheca qiui Marchán, Decäens & Domínguez, n. sp., Scherotheca litoralis Marchán, Decäens & Domínguez, n. sp., Scherotheca capcorsana Marchán, Decäens & Domínguez, n. sp. and Scherotheca mausoleana Marchán, Decäens & Domínguez, n. sp. The diversity of the genus in Corsica has thus increased from four to 12 species. In addition, Eumenescolex emiliae Qiu & Bouché, 1998 appears phylogenetically nested within the Corsican Scherotheca, justifying amendment of the name to Scherotheca emiliae (Qiu & Bouché, 1998) n. comb. leading to 13 Corsican species in the genus. We further discuss diversity patterns, phylogenetic relationships and biogeographical history of the genus in the Corsica Island.

Published
2023

7. CYTOTOXIC EVALUATION OF ORIGINAL PYRAZOLO[3,4-d]THIAZOLES AND PYRAZOLO[3,4-c]PYRAZOLES

Author

Ostache, Nicu-Cosmin, Pâris, Arnaud, Hiebel, Marie-Aude, Fînaru, Adriana-Luminita, Daniellou, Richard, Suzenet, Franck, Guillaumet, Gérald, Institut de Chimie Organique et Analytique (ICOA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University of Bacău Vasile Alecsandri, ERASMUS grants and CNFIS-FDI-0151/2017_0087/2019_0130/2020_ 0123/2021), Francophone University Agency in Central and Eastern Europe (CE_124_FF_2016), Campus France (N 857352 B, N 902192 B), the Eiffel Scholarship Program of Excellence (N 870738 C), projects CHemBio (FEDER-FSE 2014-2020-EX003677), Techsab (FEDER-FSE 2014-2020- EX011313), the RTR Motivhealth (2019-00131403), University of Orléans, CNRS, France, ANR-11-LABX-0029,SYNORG,Synthèse Organique : des molécules au vivant(2011), and ANR-11-LABX-0018,IRON,Radiopharmaceutiques Innovants en Oncologie et Neurologie(2011)

Subjects
[CHIM.ORGA]Chemical Sciences/Organic chemistry, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, General Pharmacology, Toxicology and Pharmaceutics
Abstract

International audience; A concise and versatile synthetic strategy for the synthesis of original substituted heterobicycles was developed, relying on the use of aromatic hydrazines in one-pot condensations and Ullmann-type intramolecular cyclization sequences, as well as on chemoselective brominations and palladium-mediated cross-coupling reactions. In this study, the two series of novel heterobicycles were tested for their cytotoxic potential. The compounds were structurally characterized by nuclear magnetic resonance spectrometry (1H NMR and 13C NMR), infrared spectroscopy (IR) and high-resolution mass spectrometry (HRMS). The IC50 values of these compounds on five tumour cell lines (A-549, HS-683, MCF-7, SK-MEL-28 and B16-F1) were obtained by MTT colorimetric assay. Six of the compounds exhibited excellent activity compared to 5-fluorouracil against two of the five cell lines tested, with IC50 values ranging from 0.1 to 10 μM.

Published
2022

8. Development of a Chitosan/Nickel Phthalocyanine Composite Based Conductometric Micro‐sensor for Methanol Detection

Author

Ibrahim Musa, Guy Raffin, Marie Hangouet, Marie Martin, Albert Alcacer, Nadia Zine, Francesca Bellagambi, Nicole Jaffrezic‐Renault, Abdelhamid Errachid, Micro & Nanobiotechnologies, Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), spectroscopies vibrationnelles, Centro Nacional de Microelectronica [Spain] (CNM), The authors acknowledge the financial support of the EU H2020 WIDESPREAD Program entitled Bionanosens grant agreement # 951887 and France Embassy of Nigeria, and Kano state government of Nigeria, through CAMPUS FRANCE under the MoU agreement KN-FR scholarship., and European Project: 951887,Bionanosens

Subjects
[SDV]Life Sciences [q-bio], Electrochemistry, [CHIM]Chemical Sciences, Analytical Chemistry
Abstract

International audience; Thin-film composite of chitosan/nickel phthalocyanine (NiPc) was electrochemically deposited on the fingers of interdigitated gold electrodes, applying chronoamperometric polymerization technique. The presence of crystallized NiPc in the chitosan was confirmed by EDX and FTIR analysis. Acetone, ethanol, and methanol gas-sensing properties of the films prepared at optimum conditions were studied at atmospheric temperature, through differential measurements at an optimized frequency of 10 kHz, using a lock-in amplifier. The conductometric sensor presents the highest sensitivity of 60.2 μS.cm−1(v/v) for methanol and 700 ppm as the limit of detection. For validation, the methanol content of a commercial rubbing alcohol was determined.

Published
2022

9. Photocatalytic Performance of Perovskite and Metal–Organic Framework Hybrid Material for the Reduction of N2 to Ammonia

Author

Masoumeh Chamack, Madjid Ifires, Sayed Ali Akbar Razavi, Ali Morsali, Ahmed Addad, Afsanehsadat Larimi, Sabine Szunerits, Rabah Boukherroub, Tarbiat Modares University [Tehran], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), NanoBioInterfaces - IEMN (NBI - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), and National Centre for Scientific Research (CNRS), Center for International Scientific Studies and Collaboration (GUNDISHAPUR 2019, Project No. 261), The University of Lille, the Hauts-de-France region, Tarbiat Modares University (TMU), Campus France (PHC GUNDISHAPUR 2019, Project No. 42965PK) and Niroo Research Institute (NRI) are gratefully acknowledged for financial support.

Subjects
[PHYS]Physics [physics], Inorganic Chemistry, X-ray photoelectron spectroscopy, [SPI]Engineering Sciences [physics], Nitrogen, Photocatalysts, Metal organic frameworks, Physical and Theoretical Chemistry, Composites
Abstract

International audience; The orthorhombic phase of KNbO3 perovskite has been applied for nitrogen (N-2) photoreduction to ammonia (NH3). However, this material suffers from a low surface area and low ammonia production efficiency under UV light irradiation. To eliminate these barriers, we used a metal-organic framework (MOF), named as TMU-5 ([Zn(OBA)(BPDH)(0.5)](n).1.5DMF, where H(2)OBA = 4,4'-oxybis(benzoic acid) and BPDH = 2,5-bis(4-pyridyl)-3,4-diaza-2,4-hexadiene), for the synthesis of the KNbO3@TMU-5 hybrid material. KNbO3@TMU-5 achieved a NH3 production rate of 39.9 mu mol.L-1.h(-1).g(-1) upon UV light irradiation, as compared to 20.5 mu mol.L-1.h(-1).g(-1) recorded for KNbO3 under similar experimental conditions. Using different characterization techniques especially gas adsorption, cyclic voltammetry, X-ray photoelectron spectroscopy, photocurrent measurements, and Fourier transform infrared spectroscopy, it has been found that the higher photoactivity of KNbO3@TMU-5 in ammonia production is due to its higher surface area, higher electron-hole separation efficiency, and higher density of negative charges on Nb sites. This work shows that hybridization of conventional semiconductors (SCs) with photoactive MOFs can improve the photoactivity of the SC@MOF hybrid material in different reactions, especially kinetically complex reactions like photoconversion of nitrogen to ammonia.

Published
2022

10. Atmospheric dispersion modelling of gaseous emissions from Beirutinternational airport activities

Author

Mokalled, Tharwat, Le Calvé, Stéphane, Badaro-Saliba, Nada, Abboud, Maher, Zaarour, Rita, Farah, Wehbeh, Adjizian-Gérard, Jocelyne, Institut de chimie et procédés pour l'énergie, l'environnement et la santé (ICPEES), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Saint-Joseph de Beyrouth (USJ), Unité de Recherche Environnement, Génomique Fonctionnelle et Études Mathématiques [Beyrouth] (UR-EGFEM), This research was funded by the Research Council at Saint Joseph University and supported by Strasbourg University and Campus France., and Le Calvé, Stéphane

Subjects
[SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, volatile organic compound, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Beirut, nitrogen dioxide, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDE.IE]Environmental Sciences/Environmental Engineering, [SDU.OCEAN] Sciences of the Universe [physics]/Ocean, Atmosphere, aviation emission, atmospheric dispersion modelling system (ADMS), [SDE.IE] Environmental Sciences/Environmental Engineering, General Environmental Science
Abstract

The projected increase of civil aviation activity, the degradation of air quality and the location of Beirut Airport embedded in a very urbanized area, in addition to the special geography and topography surrounding the airport which plays a significant role in drawing emissions to larger distances, demanded anassessment of the spatial impact of the airport activities on the air quality of Beirut and its suburbs. This is the first study in the Middle East region that model pollutant concentrations resulting from an international airport's activities using an advanced atmospheric dispersion modelling system in a country with no data. This followed validation campaigns showing very strong correlations (r = 0.85) at validation sites as close as possible to emission sources. The modelling results showed extremely high NO2 concentrations within the airport vicinity, i.e., up to 110 μg∙m-3 (which is greater than the World Health Organization annual guidelines) posing a health hazard to the workers in the ramp. The major contribution of Beirut–Rafic Hariri International Airport to the degradation of air quality was in the airport vicinity; however, it extended to Beirut and its suburbs in addition to affecting the seashore area due to emissions along the aircraft trajectory; this isan aspect rarely considered in previous studies. On the other hand, elevated volatile organic compound levels were observed near the fuel tanks and at the aerodrome center. This study provides (ⅰ) a methodology to assess pollutant concentrations resulting from airport emissions through the use of an advanced dispersion model in a country with no data; and (ⅱ) a tool for policy makers to better understand the contribution of the airport's operations to national pollutant emissions, which is vital for mitigation strategies and health impact assessments.

Published
2022

11. Enhancement of microbial fuel cell efficiency by incorporation of graphene oxide and functionalized graphene oxide in sulfonated polyethersulfone membrane

Author

Anthony Szymczyk, Mehri Shabani, Ahmad Rahimpour, Maxime Pontié, Hanxiao Guo, Mostafa Rahimnejad, Habibollah Younesi, Tarbiat Modares University [Tehran], ESAIP, Université d'Angers (UA), Babol Noshirvani University of Technology, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Iran National Science Foundation, INSF: 97014305, IG-39804, Campus France, Tarbiat Modares University, TMU, Universite Angers, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Microbial fuel cell, Materials science, Oxide, 02 engineering and technology, Conductivity, 010402 general chemistry, 01 natural sciences, law.invention, chemistry.chemical_compound, law, Functionalized GO, [CHIM]Chemical Sciences, Electricity generation, Renewable Energy, Sustainability and the Environment, Graphene, Proton exchange membranes, 021001 nanoscience & nanotechnology, Polyelectrolyte, 0104 chemical sciences, Membrane, chemistry, Chemical engineering, 0210 nano-technology, Selectivity, Faraday efficiency
Abstract

International audience; The present work examines, for the first time, the use of thiolated graphene oxide (TGO), in polyelectrolyte composite membranes as an effective approach to enhance the MFC performance. A new composite membrane based on a sulfonated polyethersulfone (SPES) hybrid with GO, sulfonated GO (SGO), and TGO was fabricated and assessed in MFC. The blend membranes were characterized with various techniques. The sulfhydryl (-SH) and sulfonic (-SO3H) groups enhanced the proton selectivity of the membrane and MFC performance. The MFC using the SPES/SGO1.8% composite membrane generated a power density of 66.4 mW/m2 which was double that produced by MFC using Nafion117 membrane in batch mode which lasted for 8 days. The SPES/SGO membrane was more selective towards H+ rather than other cations (K+, Na+, and Li+). This was also confirmed by the results of proton conductivity analysis, as the SPES/SGO1.8% membranes showed a value of 1.42 mS/cm which was higher than Nafion117 (1.3 mS/cm), SPES/TGO1.8% (1.25 mS/cm), SPES/GO1.8% (0.56 mS/cm), and SPES (0.32 mS/cm). The higher COD removal and coulombic efficiency were obtained in MFC with SPES/SGO membranes. In conclusion, it is our view that the new SPES/SGO and SPES/TGO membranes can be applied favorably in dual-chamber MFCs meeting their needs. © 2021 Elsevier Ltd

Published
2021

12. An updated checklist and a DNA barcode library for the earthworms (Crassiclitellata, Oligochaeta) of Corsica, France

Author

Marchán, Daniel Fernández, Gérard, Sylvain, Hedde, Mickaël, Rougerie, Rodolphe, Decaëns, Thibaud, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Muséum national d'Histoire naturelle (MNHN), naturalist expedition, 'Our Planet Reviewed – Corsica 2019-2021' organized by the Muséum national d’Histoire naturelle (MNHN, Paris) in collaboration with and funded by the Collectivité de Corse (CdC) and the Office français de la Biodiversité (OFB), and Make Our Planet Great Again Postdoctoral grant from Campus France (mopga-postdoc-3–6111272103)

Subjects
Annelida, Acanthodrilidae, Mediterranean islands, new records, species list, Biodiversity, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Hormogastridae, endemism, Crassiclitellata, Animalia, Clitellata, DNA barcoding, Animal Science and Zoology, Diporodrilidae, Lumbricidae, Ecology, Evolution, Behavior and Systematics, Taxonomy
Abstract

International audience; Available studies of the earthworm fauna of Corsica reported a total of 36 species, but this regional diversity is probably underestimated considering the relatively modest sampling efforts achieved so far. We conducted a sampling campaign in the context of the program “Our Planet Reviewed”, with the aim to increase sampling coverage and to analyze already known regional diversity with a combination of classical morpho-anatomic characters and DNA barcoding. We sampled a total of 31 locations, and identified 22 previously recorded species and eight new citations belonging to the families Acanthodrilidae Claus, 1880, Hormogastridae Michaelsen, 1900 and Lumbricidae Rafinesque- Schmaltz, 1815. We obtained 525 COI barcodes which clustered into 48 genetic lineages, highlight- ing the importance of molecular techniques for earthworm diversity surveys. Of these lineages, ten are supported by multiple lines of evidence and will be described in a forthcoming taxonomy paper as species new to science. Biogeographical affinities of the identified species showed a lower rate of cosmopolitan species compared to other Mediterranean islands, a certain degree of affinity with Italy and Sardinia, and a high rate of endemism (which could be mirrored by the Balearic Islands and Sardinia). Comparative phylogeographic analyses are needed to understand the history of coloniza- tion and diversification of earthworms on Mediterranean islands, and to investigate the drivers that shaped differences in their diversity.; Les études disponibles sur la faune lombricienne de la Corse font état d’un total de 36 espèces, mais cette diversité régionale est probablement sous-estimée si l’on considère les efforts d’échantillonnage relativement modestes réalisés jusqu’à présent. Nous avons mené une campagne d’échantillonnage dans le cadre du programme « La Planète Revisitée », avec comme objectif d’augmenter la couver- ture d’échantillonnage et d’analyser la diversité régionale en combinant l’utilisation des caractères morpho-anatomiques classiques à celle des codes-barres ADN. Nous avons échantillonné un total de 31 sites, ce qui a permis d’identifier 22 espèces précédemment citées et huit nouvelles signalisa- tions appartenant aux familles Acanthodrilidae Claus, 1880, Hormogastridae Michaelsen, 1900 et Lumbricidae Rafinesque-Schmaltz, 1815. Nous avons obtenu 525 codes-barres ADN qui nous ont permis de délimiter 48 lignées génétiques, soulignant l’importance des techniques moléculaires pour inventorier la diversité des vers de terre. Parmi ces lignées, dix sont soutenues par la morphologie et seront décrites comme espèces nouvelles pour la science dans un article de taxonomie à venir. Les affinités biogéographiques des espèces identifiées montrent un taux relativement faible d’espèces cos- mopolites par rapport à d’autres îles méditerranéennes, un degré d’affinité avec les faunes italiennes et sardes, et un taux élevé d’endémisme (qui pourrait être équivalent à celui des îles Baléares et de la Sardaigne). Des analyses phylogéographiques comparatives pourraient aider à comprendre l’histoire de la colonisation et la diversification des faunes de vers de terre des îles méditerranéennes et à com- prendre les facteurs qui ont façonné les différences dans leur diversité.

Published
2022

13. Migration collective cellulaire sur des substrats courbes cylindriques

Author

Glentis, Alexandros, Blanch-Mercader, Carles, Balasubramaniam, Lakshmi, Saw, Thuan Beng, d’Alessandro, Joseph, Janel, Sebastien, Douanier, Audrey, Delaval, Benedicte, Lafont, Frank, Lim, Chwee Teck, Delacour, Delphine, Prost, Jacques, Xi, Wang, Ladoux, Benoit, Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Mechanobiology Institute [Singapore] (MBI), National University of Singapore (NUS), CHU Lille, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Laboratoire Physico-Chimie Curie [Institut Curie] [PCC], Mechanobiology Institute [Singapore] [MBI], Institut Jacques Monod [IJM (UMR_7592)], Centre de recherche en Biologie cellulaire de Montpellier [CRBM], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL], Cellular Microbiology and Physics of Infection Group [Lille] (CMPI), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, This work was supported by the LABEX 'Who Am I?' (ANR-11-LABX-0071) (to B.L.), the European Research Council (ERC) (grant no. Adv-101019835) (to B.L.), the Marie Skłodowska-Curie Actions, Individual Fellowship, project 846449 (to W.X.), People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007–2013) under REA grant agreement no. PCOFUND-GA-2013-609102, through the PRESTIGE program coordinated by Campus France (to W.X.), funding from the Labex Who Am I? (ANR-11-LABX-0071) and the 'Initiatives d’excellence' (Idex ANR-11-IDEX-0005-02) transverse project BioMechanOE (TP5) (to W.X.), the Agence Nationale de la Recherche (ANR) 'POLCAM' ANR-17-CE13-0013 funded Glentis et al., Sci. Adv. 8, eabn5406 (2022) 14 September 2022 by the French government (to B.L.), the SystemsX EpiPhysX consortium funding (to C.B.-M.), the NR PRC LUCELL grant (ANR-19-CE13-0014-01) (to B.D.), the ANR-10-EQPX-04-01 (to F.L.), the European Regional Development Fund (FEDER-12001407) (to F.L.), the Groupama Foundation—Research Prize for Rare Diseases 2017 (to D.D. and W.X.), the ANR 'LUCELL' ANR-19-CE13-0014 funded by the French government (to B.D. and D.D.), the Fondation pour la Recherche Médicale (FRM) (to D.D.), the Université de Paris IDEX, ANR-18-IDEX-0001, funded by the French government through its 'Investments for the Future' program (to D.D.), the Human Frontier Science Program, RGP0038/2018 (to D.D., C.T.L., T.B.S., J.P., and W.X.), Lee Kuan Yew Postdoctoral fellowship and Tier 1 grant (R-397-000-320-114) from the Ministry of Education (MOE), Singapore (to T.B.S.), and the National Research Foundation, Singapore, under the Mechanobiology Institute at the National University of Singapore (to C.T.L.)., ANR-11-LABX-0071,WHO AM I,Determinants de l'Identité : de la molécule à l'individu(2011), Delacour, Delphine, and Determinants de l'Identité : de la molécule à l'individu - - WHO AM I2011 - ANR-11-LABX-0071 - LABX - VALID

Subjects
[SDV] Life Sciences [q-bio], [PHYS]Physics [physics], Multidisciplinary, [SDV]Life Sciences [q-bio]
Abstract

View the article online https://www.science.org/doi/10.1126/sciadv.abn5406; International audience; Three-dimensional collective epithelial rotation around a given axis represents a coordinated cellular movement driving tissue morphogenesis and transformation. Questions regarding these behaviors and their relationship with substrate curvatures are intimately linked to spontaneous active matter processes and to vital morphogenetic and embryonic processes. Here, using interdisciplinary approaches, we study the dynamics of epithelial layers lining different cylindrical surfaces. We observe large-scale, persistent, and circumferential rotation in both concavely and convexly curved cylindrical tissues. While epithelia of inverse curvature show an orthogonal switch in actomyosin network orientation and opposite apicobasal polarities, their rotational movements emerge and vary similarly within a common curvature window. We further reveal that this persisting rotation requires stable cell-cell adhesion and Rac-1-dependent cell polarity. Using an active polar gel model, we unveil the different relationships of collective cell polarity and actin alignment with curvatures, which lead to coordinated rotational behavior despite the inverted curvature and cytoskeleton order.

Published
2022

14. Vitamin D induced microbicidal activity against Mycobacterium bovis BCG is dependent on the synergistic activity of bovine peripheral blood cell populations

Author

Susana Flores-Villalva, Aude Remot, Florence Carreras, Nathalie Winter, Stephen V. Gordon, Kieran G. Meade, University College Dublin [Dublin] (UCD), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias [Mexico] (INIFAP), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conway Institute of Biomolecular and Biomedical Research [Dublin, Irlande], Science Foundation Ireland grant to Kieran Meade (17/CDA/4717), Irish Research Council and Campus France via the PHC ‘Ulysses’ Grant Schemes 2020 and 2017, and European Project: 731014,VetBioNet(2017)

Subjects
General Veterinary, Neutrophils, [SDV]Life Sciences [q-bio], Immunology, Mycobacteria, 25(OH)(2)D(3), Vitamin D, Microbicidal activity, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Peripheral blood leukocytes
Abstract

International audience; A growing appreciation is emerging of the beneficial role of vitamin D for health and resistance against infectious diseases, including tuberculosis. However, research has predominantly focused on murine and human species and functional data in bovines is limited. Therefore, the objective of this study was to assess the microbicidal activity and immunoregulatory effect of the vitamin D metabolite 1,25(OH)2D3 on bovine peripheral blood leukocytes (PBL) in response to Mycobacterium bovis BCG (BCG) infection using a combination of functional assays and gene expression profiling. Blood from Holstein-Friesian bull calves with low circulating levels of 25(OH)D was stimulated with 1,25(OH)2D3 for 2 h, and then infected with M. bovis BCG. Results showed that 1,25(OH)2D3 supplementation significantly increased BCG killing by on average 16 %, although responses varied between 1 % and 38 % killing. Serial cell subset depletion was then performed on PBL prior to 1,25(OH)2D3 incubation and BCG infected as before to analyse the contribution of major cell types to mycobacterial growth control. Specific antibodies and either magnetic cell separation or density gradient centrifugation of monocytes, granulocytes, CD3+, CD4+, and CD8+ T lymphocytes were used to capture each cell subset. Results showed that depletion of granulocytes had the greatest impact on BCG growth, leading to a significant enhancement of bacterial colonies. In contrast, depletion of CD4+ or CD8+ T cells individually, or in combination (CD3+), had no impact on mycobacterial growth control. In agreement with our previous data, 1,25(OH)2D3 significantly increased bacterial killing in PBL, in monocyte depleted samples, and a similar trend was observed in the granulocyte depleted subset. In addition, specific analysis of sorted neutrophils treated with 1,25(OH)2D3 showed an enhanced microbicidal activity against both BCG and a virulent strain of M. bovis. Lastly, data showed that 1,25(OH)2D3 stimulation increased reactive oxygen species (ROS) production and the expression of genes encoding host defence peptides (HDP) and pathogen recognition receptors (PRRs), factors that play an important role in the microbicidal activity against mycobacteria. In conclusion, the vitamin D metabolite 1,25(OH)2D3 improves antimycobacterial killing in bovine PBLs via the synergistic activity of monocytes and granulocytes and enhanced activation of innate immunity.

Published
2022

15. Effect of channel density, inverse solutions and connectivity measures on EEG resting-state networks reconstruction: A simulation study

Author

Sahar Allouch, Aya Kabbara, Joan Duprez, Mohamad Khalil, Julien Modolo, Mahmoud Hassan, Laboratoire Traitement du Signal et de l'Image (LTSI), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Azm Center for Research and Biotechnologies, Lebanese University [Beirut] (LU), MINDig, Lebanese Association for Scientific Research [Tripoli] (LASeR), Ecole Doctorale des Sciences et de la Technologie (EDST), Reykjavík University, and The authors would like to acknowledge the Lebanese National Council for Scientific Research (CNRS-L), the Agence Universitaire de la Francophonie (AUF), and the Lebanese University for granting Ms. Allouch a doctoral scholarship. This work was also supported by the Labex Cominlabs project PKSTIM and by the Institute of Clinical Neuroscience of Rennes (Projects EEGCog and EEGNET3). The authors would also like to thank Campus France, Programme Hubert Curien CEDRE (PROJET N° 42257YA), and the Lebanese Association for Scientific Research (LASER) for their support.

Subjects
Functional connectivity, Analytical variability, Channel density, Neurology, Cognitive Neuroscience, EEG resting-state networks, Neural mass model, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Inverse solution
Abstract

International audience; Along with the study of brain activity evoked by external stimuli, the past two decades witnessed an increased interest in characterizing the spontaneous brain activity occurring during resting conditions. The identification of connectivity patterns in this so-called "resting-state" has been the subject of a great number of electrophysiology-based studies, using the Electro/Magneto-Encephalography (EEG/MEG) source connectivity method. However, no consensus has been reached yet regarding a unified (if possible) analysis pipeline, and several involved parameters and methods require cautious tuning. This is particularly challenging when different analytical choices induce significant discrepancies in results and drawn conclusions, thereby hindering the reproducibility of neuroimaging research. Hence, our objective in this study was to shed light on the effect of analytical variability on outcome consistency by evaluating the implications of parameters involved in the EEG source connectivity analysis on the accuracy of resting-state networks (RSNs) reconstruction. We simulated, using neural mass models, EEG data corresponding to two RSNs, namely the default mode network (DMN) and dorsal attentional network (DAN). We investigated the impact of five channel densities (19, 32, 64, 128, 256), three inverse solutions (weighted minimum norm estimate (wMNE), exact low-resolution brain electromagnetic tomography (eLORETA), and linearly constrained minimum variance (LCMV) beamforming) and four functional connectivity measures (phase-locking value (PLV), phase-lag index (PLI), and amplitude envelope correlation (AEC) with and without source leakage correction), on the correspondence between reconstructed and reference networks. We showed that, with different analytical choices related to the number of electrodes, source reconstruction algorithm, and functional connectivity measure, high variability is present in the results. More specifically, our results show that a higher number of EEG channels significantly increased the accuracy of the reconstructed networks. Additionally, our results showed significant variability in the performance of the tested inverse solutions and connectivity measures. Such methodological variability and absence of analysis standardization represent a critical issue for neuroimaging studies that should be prioritized. We believe that this work could be useful for the field of electrophysiology connectomics, by increasing awareness regarding the challenge of variability in methodological approaches and its implications on reported results.

Published
2023

16. Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics

Author

Veer I. P. Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aizel, Maud Bongaerts, Fanny Delille, Lorena Kolar-Znika, Vittore F. Scolari, Sebastian Hoffmann, Edward J. Banigan, Leonid A. Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon, Dynamique du noyau [Institut Curie], Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Biologie Cellulaire et Cancer, Université Paris sciences et lettres (PSL), Department of Physics [MIT Cambridge], Massachusetts Institute of Technology (MIT), Laboratoire de Physique et d'Etude des Matériaux (UMR 8213) (LPEM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), LabEx CELL(N)SCALE, LabEx DEEP, ANR, ERC, Institut Curie, ATIP-Avenir (CNRS, INSERM, Plan Cancer), Campus France (PRESTIGE-2018-1-0023), Fondation ARC (PJA 20161204869), NIH (GM114190), MIT-France Seed Fund, Région Île-de-France (Chaire Blaise Pascal), ANR-18-CE12-0023,CHROMAG,Probing genome dynamics and mechanics at the single chromosome level(2018), ANR-11-LABX-0038,CelTisPhyBio,Des cellules aux tissus: au croisement de la Physique et de la Biologie(2011), ANR-11-LABX-0044,DEEP,Développement, Epigénèse, Epigénétique et potentiel de vie(2011), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), European Project: 757956,4D-GenEx, European Project: 666003,H2020,H2020-MSCA-COFUND-2014,IC-3i-PhD(2016), Coulon, Antoine, APPEL À PROJETS GÉNÉRIQUE 2018 - Probing genome dynamics and mechanics at the single chromosome level - - CHROMAG2018 - ANR-18-CE12-0023 - AAPG2018 - VALID, Des cellules aux tissus: au croisement de la Physique et de la Biologie - - CelTisPhyBio2011 - ANR-11-LABX-0038 - LABX - VALID, Développement, Epigénèse, Epigénétique et potentiel de vie - - DEEP2011 - ANR-11-LABX-0044 - LABX - VALID, Initiative d'excellence - Paris Sciences et Lettres - - PSL2010 - ANR-10-IDEX-0001 - IDEX - VALID, European Research Council (ERC) Starting Grant - 4D-GenEx - 757956 - INCOMING, and Institut Curie 3-i PhD Program - IC-3i-PhD - - H20202016-09-01 - 2021-09-01 - 666003 - VALID

Subjects
Cell Nucleus, Multidisciplinary, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Genomics, Chromatin, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, Micromanipulation, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Chromosomes, Human, Humans, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], Interphase
Abstract

International audience; Our understanding of the physical principles organizing the genome in the nucleus is limited by the lack of tools to directly exert and measure forces on interphase chromosomes in vivo and probe their material nature. Here, we introduce an approach to actively manipulate a genomic locus using controlled magnetic forces inside the nucleus of a living human cell. We observed viscoelastic displacements over micrometers within minutes in response to near-piconewton forces, which are consistent with a Rouse polymer model. Our results highlight the fluidity of chromatin, with a moderate contribution of the surrounding material, revealing minor roles for cross-links and topological effects and challenging the view that interphase chromatin is a gel-like material. Our technology opens avenues for future research in areas from chromosome mechanics to genome functions.

Published
2022

17. Microwave absorption by carbon-based materials and structures

Author

Polina Kuzhir, Alain Celzard, Xiaobo Chen, University of Eastern Finland, Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), University of Missouri [Kansas City] (UMKC), University of Missouri System, Academy of Finland via Flagship Program Photonics Research and Innovation (PREIN), Decision No. 32016, Campus France and Lithuanian Science Council through the joint program PHC Gilibert No. 46414VC 'Hybrid gels for electromagnetic applications,', NATO Science for Peace and Security Program [Grant No. G5697 CERTAIN, 'Globular carbon-based structures and metamaterials for enhanced electromagnetic protection']., European Project: 823728 ,DiSeTCom, and European Project: 823878,TERASSE

Subjects
[PHYS]Physics [physics], Microwave absorptions, microwave, nanocarbon, graphene, Carbohydrates, General Physics and Astronomy, Composite materials, 3D printing, metasurface, Carbon based materials, Chemical engineering, Electromagnetic spectrum, Electromagnetic compatibility, carbon nanotube, absorption, Mechanosynthesis
Abstract

'Microwave absorption by carbon-based materials and structures' special topic Editorial&nbsp

Published
2022

18. Achieving high circularly polarized luminescence with push–pull helicenic systems: from rationalized design to top-emission CP-OLED applications

Author

Bassem Jamoussi, Laura Abella, Benoit Racine, Sylvia Meunier-Della-Gatta, Thierry Roisnel, Nicolas Vanthuyne, Etienne Quesnel, Ludovic Favereau, Grégory Pieters, Kais Dhbaibi, Jeanne Crassous, Jochen Autschbach, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Synthèse Caractérisation Analyse de la Matière (ScanMAT), Université de Rennes (UR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), University at Buffalo [SUNY] (SUNY Buffalo), State University of New York (SUNY), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), King Abdulaziz University, Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Science Foundation, NSF: CHE-1855470, University at Buffalo, UB, University of Gabès, UNIVGB, Campus France, Université de Rennes 1, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut de Chimie du CNRS (INC)

Subjects
Dipole transition moment, Luminescence, Exciton, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, chemistry.chemical_compound, Crucial parameters, Stereochemistry, Theoretical investigations, OLED, [CHIM]Chemical Sciences, Relative orientation, Circular polarization, Luminescence process, business.industry, Excited states, General Chemistry, 021001 nanoscience & nanotechnology, Molecular orientation, 0104 chemical sciences, Organic light emitting diodes (OLED), Chemistry, Chiroptical properties, Dipole, Circularly polarized luminescence, Helicene, chemistry, Excited state, Optoelectronics, Mutual orientation, 0210 nano-technology, business, Magnetic dipole
Abstract

While the development of chiral molecules displaying circularly polarized luminescence (CPL) has received considerable attention, the corresponding CPL intensity, glum, hardly exceeds 10−2 at the molecular level owing to the difficulty in optimizing the key parameters governing such a luminescence process. To address this challenge, we report here the synthesis and chiroptical properties of a new family of π-helical push–pull systems based on carbo[6]helicene, where the latter acts as either a chiral electron acceptor or a donor unit. This comprehensive experimental and theoretical investigation shows that the magnitude and relative orientation of the electric (μe) and magnetic (μm) dipole transition moments can be tuned efficiently with regard to the molecular chiroptical properties, which results in high glum values, i.e. up to 3–4 × 10−2. Our investigations revealed that the optimized mutual orientation of the electric and magnetic dipoles in the excited state is a crucial parameter to achieve intense helicene-mediated exciton coupling, which is a major contributor to the obtained strong CPL. Finally, top-emission CP-OLEDs were fabricated through vapor deposition, which afforded a promising gEl of around 8 × 10−3. These results bring about further molecular design guidelines to reach high CPL intensity and offer new insights into the development of innovative CP-OLED architectures., A CPL intensity of up to 3 × 10−2 is achieved in π-extended 6-helicene derivatives, owing to an intense helicene-mediated exciton coupling. Corresponding top-emission CP-OLEDs afforded a promising gEl of around 8 × 10−3.

Published
2021

19. Strong σ ‐Hole Activation on Icosahedral Carborane Derivatives for a Directional Halide Recognition

Author

Won-Sik Han, Olivier Jeannin, Emmanuel Aubert, Sunhee Lee, Maxime Beau, Ie-Rang Jeon, Sooyeon Kim, Marc Fourmigué, Enrique Espinosa, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Seoul Women's University, Cristallographie, Résonance Magnétique et Modélisations (CRM2), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), ANR 17-ERC3-0003, Agence Nationale de la Recherche, PHC STAR 41595RK, Campus France, NRF-2018K1A3A1A21043478, National Research Foundation of Korea, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Institut Jean Barriol (IJB), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Sigma-hole interaction, Dimer, Supramolecular chemistry, Halide, Chalcogen bonding, Crystal engineering, 010402 general chemistry, 01 natural sciences, Catalysis, Chalcogen, chemistry.chemical_compound, carborane, [CHIM.CRIS]Chemical Sciences/Cristallography, [CHIM]Chemical Sciences, Molecule, Halogen bond, Chemistry, 010405 organic chemistry, General Chemistry, General Medicine, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Crystallography, crystal engineering, Carborane, Halogen bonding
Abstract

International audience; Crystal engineering based on σ -hole interactions is an emerging approach for realization of new materials with higher complexity. Neutral inorganic clusters derived from 1,2-dicarba- closo -dodecaborane, substituted with -SeMe, -TeMe, and -I moieties on both skeletal carbon vertices are experimentally demonstrated here as outstanding chalcogen- and halogen-bond donors. In particular, these new molecules strongly interact with halide anions in the solid-state. The halide ions are coordinated by one or two donor groups ( μ 1 - and μ 2 -coordinations), to stabilize a discrete monomer or dimer motifs to 1D supramolecular zig-zag chains. Crucially, the observed chalcogen bond and halogen bond interactions feature remarkably short distances and strong directionality. Electrostatic potential calculations further demonstrate the efficiency of the carborane derivatives, with V s,max being similar or even superior to that of reference organic halogen bond donors such as iodopentafluorobenzene.

Published
2020

20. Teach Your Robot Your Language! Trainable Neural Parser for Modeling Human Sentence Processing: Examples for 15 Languages

Author

Johannes Twiefel, Xavier Hinaut, Mnemonic Synergy (Mnemosyne), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS)-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Institut des Maladies Neurodégénératives [Bordeaux] (IMN), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Knowledge Technology group [Hamburg] (KT), Department of Informatics [Hamburg], University of Hamburg-University of Hamburg, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Inria Bordeaux - Sud-Ouest, and Support by a Marie Curie Intra EuropeanFellowship within the 7th European Community Framework Programme:EchoRob project (PIEF-GA-2013-627156). 'LingoRob - Learning Language in Developmental Robots' by Campus France PHC Procope Project 37857TF and by DAAD Project 57317821 in the Förderprogramm 'Projektbezogener Personenaustausch Frankreich'

Subjects
Computer science, [INFO.INFO-NE]Computer Science [cs]/Neural and Evolutionary Computing [cs.NE], 010501 environmental sciences, computer.software_genre, Semantics, 01 natural sciences, language learning, Human–robot interaction, Sentence processing, German, human-robot interaction, 03 medical and health sciences, echo state networks, 0302 clinical medicine, [INFO.INFO-LG]Computer Science [cs]/Machine Learning [cs.LG], Artificial Intelligence, [INFO.INFO-RB]Computer Science [cs]/Robotics [cs.RO], natural language processing, 0105 earth and related environmental sciences, Parsing, Artificial neural networks, business.industry, sentence processing, reservoir computing, [SCCO.LING]Cognitive science/Linguistics, Language acquisition, language.human_language, Recurrent neural network, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, language, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], recurrent neural network, Artificial intelligence, multilingual, business, computer, 030217 neurology & neurosurgery, Software, Sentence, Natural language processing
Abstract

International audience; We present a Recurrent Neural Network (RNN) that performs thematic role assignment and can be used for Human-Robot Interaction (HRI). The RNN is trained to map sentence structures to meanings (e.g. predicates). Previously, we have shown that the model is able to generalize on English and French corpora. In this study, we investigate its ability to adapt to various languages originating from Asia or Europe. We show that it can successfully learn to parse sentences related to home scenarios in fifteen languages: English, German, French, Spanish, Catalan, Basque, Portuguese, Italian, Bulgarian, Turkish, Persian, Hindi, Marathi, Malay and Mandarin Chinese. Moreover, in the corpora we have deliberately included variable complex sentences in order to explore the flexibility of the predicate-like output representations. This demonstrates that (1) the learning principle of our model is not limited to a particular language (or particular sentence structures), but more generic in nature, and (2) it can deal with various kind of representations (not only predicates), which enables users to adapt it to their own needs. As the model is inspired from neuroscience and language acquisition theories, this generic and language independent aspect makes it a good candidate for modelling human sentence processing. Finally, we discuss the potential implementation of the model in a grounded robotic architecture.

Published
2020

21. A Flexible and Original Architecture of Two Unrelated Zinc Fingers Underlies the Role of the Multitask P1 in Rymv Spread

Author

Vianney Poignavent, François Hoh, Guillaume Terral, Yinshan Yang, François-Xavier Gillet, Jeong-Hyeon Kim, Frédéric Allemand, Eric Lacombe, Christophe Brugidou, Sarah Cianferani, Hélène Déméné, Florence Vignols, UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre de Biologie Structurale [Montpellier] (CBS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), BioCampus (BCM), Laboratoire de Spectrométrie de Masse BioOrganique [Strasbourg] (LSMBO), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Microbiologie, adaptation et pathogénie (MAP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Institut des Sciences des Plantes de Montpellier (IPSIM), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Campus France Orchid program (Grant no29354SD), the Taiwanese Research and Practical Training Program, GAIA PhD School of Montpellier University, France., and ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010)

Subjects
History, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Polymers and Plastics, Oryza, Zinc Fingers, Crystallography, X-Ray, Industrial and Manufacturing Engineering, Plant Viruses, Viral Proteins, Zinc, Protein Domains, Structural Biology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Business and International Management, Molecular Biology, Nuclear Magnetic Resonance, Biomolecular
Abstract

International audience; Viruses of the sobemovirus genus are plant viruses, most of which generate very important agricultural and financial losses. Among them, the rice yellow mottle virus (RYMV) is one of the most damaging pathogens devastating rice fields in Africa. RYMV infectivity and propagation rely on its protein P1, identified as a key movement and potential long-distance RNA silencing suppressor. Here we describe P1′s complete 3D structure and dynamics obtained by an integrative approach combining X-Ray crystallography and NMR spectroscopy. We show that P1 is organized in two semi-independent and topologically unrelated domains, each harboring an original zinc finger. The two domains exhibit different affinities for zinc and sensitivities to oxidoreduction conditions, making the C-terminal P1 region a potential labile sensor of the plant redox status. An additional level of regulation resides on the capacity of P1 to oligomerize through its N-terminal domain. Coupling P1 structure information with site-directed mutagenesis and plant functional assays, we identified key residues in each zinc domain essential for infectivity and spread in rice tissues. Altogether, our results provide the first complete structure of a sobemoviral P1 movement protein and highlight structural and dynamical properties that may serve RYMV functions to infect and invade its host plant.

Published
2022

22. Enhanced TLR3 Responsiveness in Hepatitis C Virus Resistant Women From the Irish Anti-D Cohort

Author

Jamie A. Sugrue, Céline Posseme, Ziyang Tan, Christian Pou, Bruno Charbit, Vincent Bondet, Nollaig M. Bourke, Petter Brodin, Darragh Duffy, Cliona O’Farrelly, Trinity College Dublin, Immunologie Translationnelle - Translational Immunology lab, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Karolinska Institutet [Stockholm], Cytometrie et Biomarqueurs – Cytometry and Biomarkers (UTechS CB), The VirScan data handling was enabled by resources in project sens2018511 provided by the Swedish National Infrastructure for Computing (SNIC) at UPPMAX, partially funded by the Swedish Research Council through grant agreement no. 2018-05973. The authors acknowledge colleagues at the Irish Blood Transfusion Service for their help in matching donors to their batch numbers. This study was funded through a Science Foundation Ireland grant to C.O.F. (12/IA/1667) and benefitted from an Irish Research Council – Campus France Ulysses award., and The authors are grateful to the many blood donors who contributed to this study. The authors acknowledge Margaret Needham for help processing samples and the administrative support in organizing the blood donations.

Subjects
[SDV.IMM]Life Sciences [q-bio]/Immunology, Humans, Female, Hepacivirus, Hepatitis C, Antiviral Agents, General Biochemistry, Genetics and Molecular Biology, Toll-Like Receptor 3
Abstract

Cytokine data presented in this manuscript is available at https://dx.doi.org/10.17632/m65wh4vc9z.1. NanoString data is available at https://data.mendeley.com/datasets/z4nr7p7ry7. Additional data reported in this paper will be shared by the lead contact upon request.; International audience; Natural resistance to infection is an overlooked outcome after hepatitis C virus (HCV) exposure. Between 1977 and 1979, 1,200 Rhesus D-negative Irish women were exposed to HCV-contaminated anti-D immunoglobulin. Here, we investigate why some individuals appear to resist infection despite exposure (exposed seronegative [ESN]). We screen HCV-resistant and -susceptible donors for anti-HCV adaptive immune responses using ELISpots and VirScan to profile antibodies against all know human viruses. We perform standardized ex vivo whole blood stimulation (TruCulture) assays with antiviral ligands and assess antiviral responses using NanoString transcriptomics and Luminex proteomics. We describe an enhanced TLR3-type I interferon response in ESNs compared with seropositive women. We also identify increased inflammatory cytokine production in response to polyIC in ESNs compared with seropositive women. These enhanced responses may have contributed to innate immune protection against HCV infection in our cohort.

Published
2022

23. Two Argan Oil Phytosterols, Schottenol and Spinasterol, Attenuate Oxidative Stress and Restore LPS-Dysregulated Peroxisomal Functions in Acox1−/− and Wild-Type BV-2 Microglial Cells

Author

Soukaina Essadek, Catherine Gondcaille, Stéphane Savary, Mohammad Samadi, Joseph Vamecq, Gérard Lizard, Riad El Kebbaj, Norbert Latruffe, Alexandre Benani, Boubker Nasser, Mustapha Cherkaoui-Malki, Pierre Andreoletti, Laboratoire Bio-PeroxIL. Biochimie du peroxysome, inflammation et métabolisme lipidique [Dijon] (BIO-PEROXIL), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université Hassan 1er [Settat], Université de Lorraine (UL), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Laboratoire d'Hormonologie, Métabolisme-Nutrition & Oncologie (HMNO), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Département de Biochimie et Biologie Moléculaire-Centre de Biologie et Pathologie (CBP) Pierre-Marie Degand-INSERM Structures Partenaires, Maladies RAres du DEveloppement embryonnaire et du MEtabolisme : du Phénotype au Génotype et à la Fonction - ULR 7364 (RADEME), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Bourgogne Franche-Comté [COMUE] (UBFC), and This research was funded by the Ministere de l'Enseignement et de la Recherche and the CNRST. The Action Integree of the Comite Mixte Inter-universitaire Franco-Marocain (n degrees TBK 19/92 n degrees Campus France: 41501RJ) from the PHC Toubkal program, Ministere des Affaires Etrangeres, the Ministere de l'enseignement et de la Recherche.

Subjects
argan oil, LPS, schottenol, Physiology, catalase, Clinical Biochemistry, microglia, Cell Biology, Biochemistry, BV-2, inflammation, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Acyl-CoA oxidase 1, peroxisome, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Spinasterol, Molecular Biology
Abstract

Oxidative stress and inflammation are the key players in neuroinflammation, in which microglia dysfunction plays a central role. Previous studies suggest that argan oil attenuates oxidative stress, inflammation, and peroxisome dysfunction in mouse brains. In this study, we explored the effects of two major argan oil (AO) phytosterols, Schottenol (Schot) and Spinasterol (Spina), on oxidative stress, inflammation, and peroxisomal dysfunction in two murine microglial BV-2 cell lines, wild-ype (Wt) and Acyl-CoA oxidase 1 (Acox1)-deficient cells challenged with LPS treatment. Herein, we used an MTT test to reveal no cytotoxicity for both phytosterols with concentrations up to 5 µM. In the LPS-activated microglial cells, cotreatment with each of these phytosterols caused a significant decrease in intracellular ROS production and the NO level released in the culture medium. Additionally, Schot and Spina were able to attenuate the LPS-dependent strong induction of Il-1β and Tnf-α mRNA levels, as well as the iNos gene and protein expression in both Wt and Acox1−/− microglial cells. On the other hand, LPS treatment impacted both the peroxisomal antioxidant capacity and the fatty acid oxidation pathway. However, both Schot and Spina treatments enhanced ACOX1 activity in the Wt BV-2 cells and normalized the catalase activity in both Wt and Acox1−/− microglial cells. These data suggest that Schot and Spina can protect cells from oxidative stress and inflammation and their harmful consequences for peroxisomal functions and the homeostasis of microglial cells. Collectively, our work provides a compelling argument for the protective mechanisms of two major argan oil phytosterols against LPS-induced brain neuroinflammation.

Published
2023

24. Variability of Bacterial Homopolysaccharide Production and Properties during Food Processing

Author

Marion Nabot, Marie Guérin, Dharini Sivakumar, Fabienne Remize, Cyrielle Garcia, Démarche intégrée pour l'obtention d'aliments de qualité (UMR QualiSud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Avignon Université (AU)-Université de La Réunion (UR)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Qualisud - Pôle de La Réunion (Qualisud Réunion ), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Avignon Université (AU)-Université de La Réunion (UR)-Institut Agro Montpellier, Tshwane University of Technology [Pretoria] (TUT), Sciences Pour l'Oenologie (SPO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, and France-South Africa from Campus France, PHC PROTEA 201942165TF

Subjects
General Immunology and Microbiology, [SDV]Life Sciences [q-bio], natural polymers, exopolysaccharide, rheology, General Agricultural and Biological Sciences, texture, General Biochemistry, Genetics and Molecular Biology
Abstract

International audience; Simple Summary Bacteria can produce diverse homopolysaccharides (HoPSs), which are polymers of glucose, fructose or galactose. The synthesis of these compounds is catalyzed by glycosyltransferases. The range of HoPS sizes is very large and mostly depends on the carbon source in the medium and the catalyzing enzyme; however, factors such as nitrogen nutrients, pH, water activity, temperature and duration of bacterial culture also impact size and yield. The sequence of the polymerization enzyme influences the structure of the HoPS, by modulating the type of linkage between monomers, both for the linear chain and for the ramifications. HoPSs' size and structure influence its rheological properties. As a consequence, the choice of catalyzing enzyme and the modulation of environmental factors open ways to guide the production of specific HoPSs in foods by bacteria. This approach presents many advantages to propose applications of bacterial HoPS to texture foods, either by in situ or ex situ production, but requires a better knowledge of HoPS production conditions. Various homopolysaccharides (HoPSs) can be produced by bacteria: alpha- and beta-glucans, beta-fructans and alpha-galactans, which are polymers of glucose, fructose and galactose, respectively. The synthesis of these compounds is catalyzed by glycosyltransferases (glycansucrases), which are able to transfer the monosaccharides in a specific substrate to the medium, which results in the growth of polysaccharide chains. The range of HoPS sizes is very large, from 10(4) to 10(9) Da, and mostly depends on the carbon source in the medium and the catalyzing enzyme. However, factors such as nitrogen nutrients, pH, water activity, temperature and duration of bacterial culture also impact the size and yield of production. The sequence of the enzyme influences the structure of the HoPS, by modulating the type of linkage between monomers, both for the linear chain and for the ramifications. HoPSs' size and structure have an effect on rheological properties of some foods by their influence on viscosity index. As a consequence, the control of structural and environmental factors opens ways to guide the production of specific HoPS in foods by bacteria, either by in situ or ex situ production, but requires a better knowledge of HoPS production conditions.

Published
2021

25. Surface Enhancement Using Black Coatings for Sensor Applications

Author

Martin Hruška, Joris More-Chevalier, Přemysl Fitl, Michal Novotný, Petr Hruška, Dejan Prokop, Petr Pokorný, Jan Kejzlar, Virginie Gadenne, Lionel Patrone, Martin Vrňata, Jan Lančok, University of Chemistry and Technology Prague (UCT Prague), Institute of Physics of the Czech Academy of Sciences (FZU / CAS), Czech Academy of Sciences [Prague] (CAS), Charles University [Prague] (CU), Institut des Matériaux, de Microélectronique et des Nanosciences de Provence (IM2NP), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), and Authors (M.H., J.M.-C., P.F., M.N., J.L., M.V., J.K. and L.P.) acknowledge the support of the Ministry of Education, Youth and Sports of the Czech Republic projects SOLID21 CZ.02.1.01/0.0/0.0/16_019/0000760, No. 8F21008 and 8J22FR023, and project No. JP22420 from the International Visegrad Fund. The authors (M.H., P.F., M.V. and M.N.) also acknowledge the support of the Czech Science Foundation (Project No. 22-14886S). The author (J.M.-C.) acknowledges the support of the Czech Science Foundation (Project No. 21-09685S). Funding from PHC Barrande 2022 (48110YA), Campus France, Ministères de l’Europe et des Affaires étrangères (MEAE) et de l’Enseignement supérieur, de la Recherche et de l’Innovation (MESRI) is acknowledged by authors (L.P., V.G., P.F. and M.H.)

Subjects
sensor applica- tions, [PHYS]Physics [physics], black aluminium, [SPI]Engineering Sciences [physics], sputtering depositions, General Chemical Engineering, black gold, evaporation depositions, nanostructured materials, QCM sensors, sensor applications, General Materials Science
Abstract

International audience; The resolution of a quartz crystal microbalance (QCM) is particularly crucial for gas sensor applications where low concentrations are detected. This resolution can be improved by increasing the effective surface of QCM electrodes and, thereby, enhancing their sensitivity. For this purpose, various researchers have investigated the use of micro-structured materials with promising results. Herein, we propose the use of easy-to-manufacture metal blacks that are highly structured even on a nanoscale level and thus provide more bonding sites for gas analytes. Two different black metals with thicknesses of 280 nm, black aluminum (B-Al) and black gold (B-Au), were deposited onto the sensor surface to improve the sensitivity following the Sauerbrey equation. Both layers present a high surface roughness due to their cauliflower morphology structure. A high response (i.e., resonant frequency shift) of these QCM sensors coated with a black metal layer was obtained. Two gaseous analytes, H2O vapor and EtOH vapor, at different concentrations, are tested, and a distinct improvement of sensitivity is observed for the QCM sensors coated with a black metal layer compared to the blank ones, without strong side effects on resonance frequency stability or mechanical quality factor. An approximately 10 times higher sensitivity to EtOH gas is reported for the QCM coated with a black gold layer compared to the blank QCM sensor.

Published
2022

26. Common Mode and Differential Mode noise of AC/DC LED Driver

Author

Abdelhakim Zeghoudi, Amar Tilmatine, Abdelber Bendaoud, Helima Slimani, Laurent Canale, Applications of Plasma, Electrostatics and Electromagnetic Compatibility (APELEC), Université Djilali Liabès [Sidi-Bel-Abbès], Lumière et Matière (LAPLACE-LM), LAboratoire PLasma et Conversion d'Energie (LAPLACE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Université Ibn Khaldoun de Tiaret = University of Tiaret, and This research is part of the PHC 'MAGHREB' (Programme Hubert Curien n°43981ZG) MELINA (Mastering Efficient Lighting In North Africa) granted to Laurent Canale and funded via Campus France by the French Ministry of Europe and Foreign Affairs and Algerian Ministry of Higher Education and Scientific Research.

Subjects
AC/DC PFC, Computer science, Driver, Topology (electrical circuits), Power factor, 7. Clean energy, Electromagnetic interference, Common Mode, Interference (communication), LISN, EMI, EMC, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Common-mode signal, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, business.industry, LED, [SPI.NRJ]Engineering Sciences [physics]/Electric power, Electrical engineering, Electromagnetic compatibility, Line (electrical engineering), Differential Mode, [SPI.TRON]Engineering Sciences [physics]/Electronics, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, business
Abstract

International audience; Electromagnetic interference (EMI) represents one of the biggest problems facing the production of any electronic device. The impact on the performance of the device caused such unwanted interference must be carefully measured to understand and quantify the electromagnetic compatibility (EMC) of the instrument under test. The electrical power conversions are among the sources of electromagnetic pollution, they provide a low power factor and generate electromagnetic interference (EMI). The white light emitting diode (LED) has attracted a lot of attention from industry and scientific researchers due to its high efficiency, easy of driving, and long life. Nevertheless, LED driver can interfere with the electronic device system due to rapid voltage variation and current. In this paper we simulate some LED driver topology on LTspice software in order to measure the electromagnetic disturbances using LISN (line impedance stabilizer network) device.

Published
2021

27. Synthesis and chiroptical properties of organometallic complexes of helicenic N ‐heterocyclic carbenes

Author

Jeanne Crassous, Thierry Roisnel, Nesrine Hafedh, Nicolas Vanthuyne, Faouzi Aloui, Marion Jean, Elsa Caytan, Ludovic Favereau, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Monastir - University of Monastir (UM), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), 16-CE07-0019-01-HEL-NHC, Agence Nationale de la Recherche, Campus France, Chirafun CNRS network, ANR-16-CE07-0019,HEL-NHC,Carbènes N-hétérocycliques (NHC) hélicéniques: synthèse, structure, propriétés photophysiques et activité catalytique(2016), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)

Subjects
Circular dichroism, chemistry.chemical_element, 010402 general chemistry, Transfer hydrogenation, 01 natural sciences, Medicinal chemistry, Catalysis, Analytical Chemistry, Rhodium, helicenes, chemistry.chemical_compound, Drug Discovery, [CHIM]Chemical Sciences, N-heterocyclic carbenes, Iridium, Spectroscopy, Pharmacology, 010405 organic chemistry, Organic Chemistry, Diastereomer, chiroptical properties, iridium, 0104 chemical sciences, chemistry, transfer hydrogenation, Enantiomer, Carbene
Abstract

International audience; Novel [4, 6]helicenes (4a,b) bearing a fused imidazolium unit have been prepared from [4, 6]helicene-2,3-di-n-propyl-amines 3a,b. The in situ formation of N-heterocyclic carbene (NHC) derivatives followed by their complexation to iridium(I) or rhodium(I) gave access to complexes 1a, 1'a, and 1b, containing mono-coordinated helicene-NHC, chloro and COD (COD = 1,5-cyclooctadiene) ligands. Ir and Rh complexes 1a and 1'a were characterized by X-ray crystallography. HPLC and NMR analyses showed that Ir(I) complex 1b existed as a mixture of two diastereomeric complexes corresponding to enantiomeric pairs M-(-)/P-(+)-1b and M-(-)/P-(+)-1b which differ by the position of COD through space. The chiroptical properties (electronic circular dichroism and optical rotation) of the four stereoisomers were measured. These complexes were also tested as catalysts in a transfer hydrogenation reaction.

Published
2019

28. Presence of bluetongue and epizootic hemorrhagic disease viruses in Egypt in 2016 and 2017

Author

Stéphan Zientara, Corinne Sailleau, Mohamed Abd El-Fatah Mahmoud, Emmanuel Bréard, Cyril Viarouge, Sahar Ahmed, Department of Cell Biology, Genetic Engineering and Biotechnology Division, National Research Centre - NRC (EGYPT)-National Research Centre - NRC (EGYPT), Department of Parasitology and Animal Diseases, Veterinary Research Division, Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), EMHOTEP Program-Campus France 35156PK, European Project: 727393,PALE Blu, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and European Project: 727393,H2020,H2020-EU.3.2.1.1.,PALE-Blu(2017)

Subjects
0301 basic medicine, Microbiology (medical), Serotype, 030106 microbiology, Cattle Diseases, Hemorrhagic Disease Virus, Epizootic, EHDV, Microbiology, 03 medical and health sciences, Genetics, Animals, Epizootic hemorrhagic disease, Elisa method, Molecular Biology, Phylogeny, Ecology, Evolution, Behavior and Systematics, 2. Zero hunger, biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Virology, Genome detection, 3. Good health, 030104 developmental biology, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Herd, biology.protein, RNA, Viral, Cattle, ELISA, Egypt, Antibody, Bluetongue virus, BTV
Abstract

BTV and EHDV are closely-related orbiviruses that are transmitted between domestic and wild ruminants via the bites of hematophagous midges. Previous studies have reported seropositivity against BTV antibodies in sheep and goats in two Egyptian governorates (Beni Suef and Menoufia). However, no recent data are available on the BTV serotype(s) circulating in Egypt and the likely presence of EHDV has never been explored. This study investigated the presence of BTV and EHDV among cattle which had been found BTV-seropositive by ELISA method. These cattle living in proximity to sheep and goats previously found BTV-seropositive. These cattle displayed no clinical signs of BT but reproductive problems had been reported in herds. A total of 227 cattle blood samples were therefore collected in 2016 and 2017. Ninety-four of the 227 animals tested by a BTV ELISA were positive for BTV antibodies (41.4%). Of these 94 ELISA-positive cattle, only 83 EDTA-blood samples were available and therefore tested for BTV and EHDV genome detection by RT-PCR and sequencing. Of the cattle sampled in 2016, results revealed that two were RT-PCR-positive for BTV and seven for EHDV. Sequencing showed the presence of EHDV-1 and BTV-3 genome sequences. EHDV-1 S2 shared 99.5% homology with an EHDV-1 S2 from a strain isolated in 2016 in Israel. BTV-3 S2 and S8 sequences shared >99.8% nucleotide similarity with the BTV-3 Zarzis S2 and S8 sequences (Tunisian BTV, also detected in 2016). Of the 66 blood samples tested following their collection in 2017, they were all EHDV-negative by RT-qPCR while five were BTV- positive by RT-qPCR. However, attempts to identify the BTV serotype of these five samples were unsuccessful. Only part of BTV S8 was sequenced and it showed 79% nucleotide similarity with S8 of atypical BTV serotypes (particularly with BTV-26 and another BTV serotype strain isolated from a sheep pox vaccine). Overall, these findings demonstrate that both BTV and EHDV were circulating in Egypt in 2016 and 2017.

Published
2019

29. Pyrogenic carbon content and dynamics in top and subsoil of French forests

Author

Samuel Abiven, Laure Soucémarianadin, Lauric Cécillon, Pierre Barré, Manuel Nicolas, Moritz Reisser, Laboratoire de géologie de l'ENS (LGENS), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Département des Géosciences - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Department of Geography, Soil Science and Biogeochemistry Unit, Universität Zürich [Zürich] = University of Zurich (UZH)-Universität Zürich [Zürich] = University of Zurich (UZH), Étude et compréhension de la biodiversité (ECODIV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Office National des Forêts (ONF), Agence de l'environnement et de la maitrise de l'energie [FR, APR REACCTIF, piCaSo project] Campus France [FR] PRESTIGE-2015-3-0008Ville de Paris [FR, 'Emergences' program, SOCUTE project] Swiss National Science Foundation [CH, National Research Program 68 'Soil as a Resource'] University of Zurich [CH, and Research Priority Programme, 'Global Change and Biodiversity']

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Topsoil, Chemistry, Soil Science, chemistry.chemical_element, Soil classification, 04 agricultural and veterinary sciences, Soil carbon, Soil type, 15. Life on land, Microbiology, Subsoil, Benzene polycarboxylic acids, Pyrogenic carbon, Environmental chemistry, Soil water, Solubilization, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Soil horizon, Degradation (geology), Forest soils, Carbon
Abstract

Pyrogenic carbon (PyC) may leave the soil surface where it is deposited, either through degradation, lateral transport or transfer within the profile. However, PyC has been seldom measured in the subsoil. We estimated the quantity and molecular composition of PyC in the topsoil and subsoil of 22 French forests with diverse soil types and vegetation cover. While the absolute PyC content decreased with depth, its proportion to the soil organic carbon remained constant or tended to increase. The benzene polycarboxylic acids pattern indicated that more condensed structures were found in the subsoil. Our results show that PyC transfers through the soil profile, probably as soluble fraction, and tends to accumulate in the subsoil, specifically in podzolic soils.

Published
2019

30. Role of Backyard Flocks in Transmission Dynamics of Highly Pathogenic Avian Influenza A(H5N8) Clade 2.3.4.4, France, 2016–2017

Author

Audrey Schmitz, Luc Robertet, Eric Niqueux, Marie Souvestre, Claire Guinat, Nicolas Eterradossi, Guillaume Croville, Mathilde Paul, Anne Bronner, Jean-Luc Guérin, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Ploufragan - Plouzané, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Direction Générale de l'Alimentation (DGAL), This project was implemented through the Chaire de Biosécurité Aviaire, granted by the French Ministry of Agriculture (Direction Générale de l’Alimentation). The research leading to these results received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. PCOFUND-GA-2013-609102, through the PRESTIGE Programme coordinated by Campus France., European Project: 609102,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,PRESTIGE(2014), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
Epidemiology, [SDV]Life Sciences [q-bio], animal diseases, Biosecurity, lcsh:Medicine, HPAI, medicine.disease_cause, Disease Outbreaks, law.invention, 0302 clinical medicine, Seroepidemiologic Studies, law, Role of Backyard Flocks in Transmission Dynamics of Highly Pathogenic Avian Influenza A(H5N8) Clade 2.3.4.4, France, 2016–2017, Odds Ratio, risk factors, Influenza A Virus, H5N8 Subtype, 030212 general & internal medicine, Clade, Avian influenza virus, poultry, Dispatch, France, H5N8, backyard flocks, biosecurity, clade 2.3.4.4, highly pathogenic avian influenza, influenza, viruses, zoonoses, Infectious Diseases, Transmission (mechanics), Microbiology (medical), Farms, Highly pathogenic, 030231 tropical medicine, Enzyme-Linked Immunosorbent Assay, Biology, History, 21st Century, lcsh:Infectious and parasitic diseases, Birds, 03 medical and health sciences, medicine, Animals, lcsh:RC109-216, Poultry Diseases, lcsh:R, Virology, Influenza A virus subtype H5N1, Molecular Typing, Influenza in Birds, Flock
Abstract

International audience; Highly pathogenic avian influenza A(H5N8) clade 2.3.4.4 spread in France during 2016-2017. We assessed the biosecurity and avian influenza virus infection status of 70 backyard flocks near H5N8-infected commercial farms. One flock was seropositive for clade 2.3.4.4. Backyard flocks linked to commercial farms had elevated risk for H5 infection.

Published
2019

31. Different groups of nitrite-reducers and N2O-reducers have distinct ecological niches and functional roles in West African cultivated soils

Author

Féline L. Assémien, Jean T. Gonnety, Amélie A. M. Cantarel, Alessandro Florio, Xavier Le Roux, Thomas Pommier, Catherine Lerondelle, UMR 1418 LEM Ecologie Microbienne. Centre de recherche Auvergne-Rhône-Alpes, Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Laboratoire Biocatalyse and Bioprocess, Unité Formation et Recherche Scientifique et Technologie des Aliments, Université Nangui Abrogoua, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), PAES UEMOA program P-Z1 - IAD - 002, Institut National de la Recherche Agronomique (INRA, EFPA Department), CAMPUS France program (AMRUGE-CI/SCAC), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)

Subjects
dénitrification, sol, [SDV]Life Sciences [q-bio], Microorganism, Soil Science, Biology, Microbiology, soil, Crop, chemistry.chemical_compound, Nitrate, nosZ, nitrate, carbone organique du sol, nirK, nirS, 2. Zero hunger, Ecological niche, Soil organic carbon, Niche differentiation, 04 agricultural and veterinary sciences, Soil carbon, Soil nitrate, 15. Life on land, Agronomy, chemistry, 13. Climate action, Soil water, Denitrification, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Mulch
Abstract

Increasing attention has been paid to microorganisms able to produce nitrous oxide (N2O), a potent greenhouse gas, or reduce it to harmless N2. Based on previous studies, niche differentiation could exist between nirK- and nirS-nitrite reducers and nosZI- and nosZII-N2O reducers, and nosZII-bacteria would have a key role for N2O reduction in soils. Most previous studies have been performed for agricultural systems but never in the moist savanna zone which covers half a million km2 in West Africa and whose soils are among the poorest in nitrogen (N) on earth. Here, we quantified potential gross and net N2O production rates along with the abundances of nirK-, nirS-, nosZI- and nosZII-harbouring bacteria for soils under six agricultural practices with maize rotations (slash-and-burn, chemical fertilization, mulching with or without inclusion of crop legumes, and without any input) after 4 and 5 crop cycles at nine sites in Ivory Coast. Sites and practices influenced denitrifier abundances and activities, the ratio of total abundances of nitrite-to-N2O reducers being highest and lowest for the mulching + green soya and slash-and-burn practices, respectively. Using structural equation modelling, we showed that nirS- and nosZI-bacteria both strongly depended on nitrate availability whereas nirK- and nosZII-bacteria were related to soil organic carbon and pH. Furthermore, potential gross and net N2O production rates depended strongly and only on the abundances of nirS- and nosZI-bacteria. Our results support the view of a clear niche differentiation between these four microbial groups but invalidate the assumption of a prominent functional role of soil nosZII-N2O reducers.

Published
2019

32. A new highly sensitive micro-sensor for the ultra-traces analysis of paracetamol directly in water

Author

Lydia Taouri, Mustapha Bourouina, Saliha Bourouina, Didier Hauchard, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Abderrahmane Mira [Béjaïa], and The authors acknowledge the financial support of the Algerian Ministry of Education and Scientific Research and Campus France program under grant agreement PROFAS B+

Subjects
Detection, Paracetamol, Cavity microelectrode, Water, [CHIM]Chemical Sciences, Spectroscopy, Electrochemical preconcentration, Analytical Chemistry
Abstract

International audience; In the present work, a simple, inexpensive and highly sensitive electrochemical micro-sensor was developed for trace detection of paracetamol (PAR) directly in water, using electrochemical preconcentration technique (EP) on a cavity microelectrode modified with carbon black nanoparticles (nCB/ CME). The results demonstrate that with an EP of PAR on nCB/ CME at 0 V for 900 s in BRS 0.04 M at pH = 7, the new proposed micro-sensor show two large linear response in ranges of 5 × 10-10 to 9 × 10-8 mol.L-1 and from 1 × 10-7 to 1 × 10-6 mol.L-1. The detection and quantification limits are estimated to be 1.4 × 10-10 and 4.3 × 10-10 mol.L-1, respectively. The performances of the new proposed nCB/ CME for the determination of PAR were compared to its performances without EP and the results reveal the importance of the application of EP in improving the electroanalytical characteristics. The applicability of the designed micro-sensor was successfully applied for the direct analysis of local tap water and treated wastewater samples spiked with trace PAR compounds with a satisfactory recovery (less than 4 %) and a relation standard deviation less than 5 %. It indicates that the electrochemical micro-sensors are an effective device for monitoring PAR concentrations directly in water without any pretreatment. © 2022 Elsevier B.V.

Published
2022

33. Water treatment intensification using a monophasic hybrid process coupling nanofiltration and ozone/hydrogen peroxide advanced oxidation

Author

Sara Ouali, Pierre-François Biard, Patrick Loulergue, Rukun You, Noureddine Nasrallah, Rachida Maachi, Anthony Szymczyk, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université des Sciences et de la Technologie Houari Boumediene = University of Sciences and Technology Houari Boumediene [Alger] (USTHB), and The Ministry of Higher Education and Scientific Research of Algeria and Campus France are gratefully acknowledged for providing S. Ouali with a Profas B + grant (954610C). The Rennes 1 university is also acknowledged for providing PF Biard with a 'Défi scientifique' grant.

Subjects
Micropollutant, Ozone, General Chemical Engineering, Organic membrane, [CHIM]Chemical Sciences, Environmental Chemistry, General Chemistry, Advanced oxidation process, Hydrogen peroxide, Nanofiltration, Industrial and Manufacturing Engineering
Abstract

International audience; This study assesses the feasibility of designing a hybrid ozonation / nanofiltration membrane process that can simultaneously oxidize and reject contaminants while exhibiting reduced membrane fouling. An innovative monophasic configuration, in which a pre-ozonated water was mixed at the membrane cell inlet to the water to be treated doped with H2O2 (at equimolar concentrations of H2O2 and O3), was implemented, allowing to avoid an ozone-enriched gas flow in the membrane cell. Thus, two commercial polymer membranes, NP10 (polyethersulfone) and NF270 (polyamide), were assessed in a cross-flow configuration despite their low-ozone compatibility compared with ceramic membranes. Ozone removal yields>90% were obtained whatever the studied water matrix. Fast ozone decomposition initiation reactions with H2O2 and with some moieties of the natural organic matter were responsible for a low ozone lifetime in the liquid bulk, allowing to protect the membranes from ozone and radicals. Deethylatrazine (DEA) was used as a radical tracer, allowing to determine Rct values with orders of magnitude of 10-6 and 10-7 in drinking water and a river water sample, respectively. The concentrations of two pharmaceuticals, carbamazepine and sulfamethoxazole, in the permeate and the retentate were lower than the detection limit in drinking water when the PES membrane was tested. During treatment of the river water sample, membrane fouling dropped by a factor two while there was no alteration of both the PES and PA membranes. Finally, thanks to synergistic effects induced by contaminant oxidation and rejection, dissolved organic content and DEA were both removed at around 70% when PA membrane, exhibiting a tighter microstructure than PES, was used.

Published
2022

34. Structure and Properties of Gallium-Rich Sodium Germano-Gallate Glasses

Author

Younes Messaddeq, Thierry Cardinal, Yannick Ledemi, Karen S. Levin, Marc Dussauze, Evelyne Fargin, Tea Skopak, Scott Kroeker, Raphaël Méreau, Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre d'Optique, Photonique et Laser (COPL), Université Laval [Québec] (ULaval), Department of Chemistry, University of Manitoba [Winnipeg], Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This research was supported by the Canada Excellence Research Chair program (CERC) in Photonics Innovations and the Aquitaine Region. The Fonds de Recherche Québecois sur la Nature et les Technologies (FRQNT, no. 097991), the Natural Sciences and Engineering Research Council (NSERC) of Canada, and the Canada Foundation for Innovation (CFI, no. 29191 and 32523) for the financial support. Access to the 21.1 T NMR spectrometer was provided by the National Ultrahigh-Field NMR Facility for Solids (Ottawa, Canada), a national research facility funded by a consortium of Canadian universities, supported by the National Research Council of Canada and Bruker Biospin, and managed by the University of Ottawa. The research program was also supported by the financial support from the French National Research Agency (ANR) in the frame of 'the Investments for the future' Programme IdEx Bordeaux—LAPHIA (ANR-10-IDEX-03-02), as well as in the frame of the ANR program no. ANR-17-CE08-0042-01 and the French Region Nouvelle Aquitaine, with the program no. 2016-1R10107. We are grateful to Dr Victor Terskikh for valuable assistance with the NMR experiments. Computer time was provided by the 'Pôle Modélisation' HPC facilities of the Institut des Sciences Moléculaires UMR 5255 CNRS—Université de Bordeaux, cofunded by the 'Nouvelle Aquitaine' region. Mobilities were supported by the CNRS (LIA LuMAQ) and by grants of the French Consulate in Québec (Frontenac Program), the association of Campus France and Mitacs Globalink., ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), and ANR-17-CE08-0042,PROTEus,ImPRession laser de fibres exOtiques Multi-MaTEriaux(2017)

Subjects
Materials science, chemistry.chemical_element, Infrared spectroscopy, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, symbols.namesake, Magic angle spinning, Germanate, Physical and Theoretical Chemistry, Gallium, glass structure, 71Ga NMR spectroscopy, [CHIM.MATE]Chemical Sciences/Material chemistry, Gallate, 021001 nanoscience & nanotechnology, vibrational spectroscopy, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Amorphous solid, 71 Ga NMR spectroscopy, General Energy, chemistry, germano-gallate glasses, infrared, symbols, Physical chemistry, 0210 nano-technology, Glass transition, Raman spectroscopy
Abstract

International audience; Glass compositions in the gallium-rich region of the ternary GaO3/2–GeO2–NaO1/2 vitreous system are studied as a function of the Na/Ga cationic ratio (ranging from 1.30 to 1.61) for a fixed GeO2 content. Glass structures are investigated by 71Ga magic angle spinning nuclear magnetic resonance, infrared, and Raman spectroscopies, and the thermal, optical, and physical properties are characterized. Vibrational spectra are interpreted with the help of density functional theory calculations. Gallium oxide generally enters the germania network in fourfold coordination, however, for a Na/Ga ratio below unity, gallium cations tend to charge balance with the formation of five- or six-fold coordination units. When the amount of sodium is greater than gallium, nonbridging oxygens are formed preferably on germanate tetrahedral units. These structural descriptions are used to understand the evolution of glass properties such as glass transition temperature, density, and refractive index.

Published
2018

35. Beyond the on/off chip trade-off: A reversibly sealed microfluidic platform for 3D tumor microtissue analysis

Author

Valérie Taly, Antoine Riaud, Gabriele Pitingolo, Philippe Nizard, Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique (MEPPOT - U1147), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe labellisée Ligue contre le Cancer, This work was supported by the Ministère de l’Enseignement Supérieur et de la Recherche, the Université Paris-Descartes, the Centre National de la Recherche Scientifique (CNRS), the Institut National de la Santé et de la Recherche Médicale (INSERM), the Institut National du Cancer (INCA) and canceropole Ile-de-France (no. 2016-1-EMERG-54-UP 5-1), the SIRIC CARPEM, the Fondation de la Recherche Medicale (SPF20160936257) and the ligue nationale contre le cancer (LNCC, Program 'Equipe labelisée LIGUE', no. EL2016.LNCC/VaT). The authors acknowledges the financial support of HTE Program-HetColi network, INSERM Physicancer program (no. PC201423) and the SIRIC CARPEM. Financial supports from CAMPUS FRANCE (n° 39525QJ) and Università Italo-Francese (n° G18_208) are gratefully acknowledged. The authors would like to thank Saksham Sharma for his important contribution to the development of the numerical models. This work has benefited from the facility and expertise of the electron microscopy facility of Institut de Biologie Paris-Seine (IBPS/FR3631 - UPMC/CNRS- Paris, France). This work was carried out with the support of the Pierre-Gilles de Gennes Institute equipment ('Investissements d’Avenir' program, reference: ANR 10-NANO 0207)., and TALY, Valerie

Subjects
0301 basic medicine, Materials science, Growth kinetics, [SDV]Life Sciences [q-bio], Microfluidics, Nanotechnology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, 02 engineering and technology, Fluorescence, SEM analysis, 03 medical and health sciences, 3D cell culture, Tumor microtissue, Materials Chemistry, Drug response, Electrical and Electronic Engineering, Modular chip, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Instrumentation, Fluorescence staining, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Metals and Alloys, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Chip, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, [SDV] Life Sciences [q-bio], Spheroid, 030104 developmental biology, Microfluidic chip, [SDV.IB]Life Sciences [q-bio]/Bioengineering, 0210 nano-technology
Abstract

International audience; Nowadays, microfluidic 3D cell culture is widely used to mimic complex microtissue and dynamic environment, performing more realistic in vitro assays for drug testing. Herein, we developed a novel microfluidic platform for tumor microtissue culture, drug response analysis and versatile microscopic characterization. By reversibly bonding the chip, we go beyond the on/off chip tradeoff, which allows us to perform both fluorescence and SEM characterization of tumor microtissues on a simple platform. The microfluidic chip consists of spherical microwells connected via microchannels, bonded through a magnetic system. Colorectal cancer HT-29 cells were cultured as spherical microtissues on chip and their growth kinetics monitored. The cytotoxic activity of Camptothecin was evaluated by in situ live/dead fluorescence staining and quantification of morphology parameters. Finally, we demonstrated the possibility to collect the 3D tumor microtissues and characterize their surface damaged by the drug using scanning electron microscopy. This reversibly sealed microfluidic platform thus enables to grow sets of 3D tumor microtissues in a controlled dynamic microenvinroment, and subsequently to retrieve the 3D tumor microtissues after chemotherapeutic treatment for in-depth analysis.

Published
2018

36. Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate

Author

Jose G. Montoya, Hélène Guegan, Rares T. Olariu, Branko Bobić, Cindy Press, Jelena Srbljanović, Kjerstie Olson, Olgica Djurković-Djaković, Florence Robert-Gangneux, Tijana Štajner, Institut de recherche en santé, environnement et travail (Irset), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA), University of Belgrade [Belgrade], Palo Alto Medical Foundation, Victor Babeş University of Medicine and Pharmacy (UMFT), CHU Pontchaillou [Rennes], Ministry of Education, Science and Technological Development of Serbia, 344 through project no. III 41019Institute for Medical Research (contract no. 451-03-345 68/2020-14/200015)Campus France (PHC Pavle Savic no.40734WC) (Serbian project number 451-03-01963/2017-09/15), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and Chard-Hutchinson, Xavier

Subjects
0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Diagnostic methods, IgM, 030106 microbiology, 030231 tropical medicine, Toxoplasma gondii, serology, Western blot, [SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics, Serology, 03 medical and health sciences, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, parasitic diseases, medicine, Seroconversion, Pregnancy, neonatal diagnosis, biology, Obstetrics, business.industry, Diagnostic test, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, medicine.disease, biology.organism_classification, maternal treatment, Congenital toxoplasmosis, 3. Good health, [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, [SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics, qPCR, Real-time polymerase chain reaction, congenital toxoplasmosis, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, business, IgA
Abstract

Neonatal diagnosis of congenital toxoplasmosis is based on a combination of serological and molecular tests. Maternal screening and treatment differ according to national policies and may impact the sensitivity of diagnostic methods in infants at birth. In this multicenter study, 115 neonates born to 61 treated (53%) and 54 (47%) untreated women were retrospectively included in three centers (France, Serbia, and the United States) to assess the impact of maternal anti-Toxoplasma treatment on the performance of neonatal workup at birth (neosynthesized anti-Toxoplasma IgM, IgA, and IgG and quantitative PCR [qPCR]) using univariate and multivariate approaches. Independently of the time of maternal seroconversion, the serological techniques were impacted differently by maternal treatment. The detection of IgM by immunosorbent agglutination assay (ISAGA) and Western blotting (WB) dropped from 90.7% and 88.2% in untreated neonates to 533% and 51.9% in treated neonates (P lt 0.05), whereas IgM enzyme-linked immunosorbent assay (ELISA) and IgA ISAGA were not significantly affected by maternal treatment. A 2-fold reduction in the sensitivity of neosynthesized IgG by WB was also observed in the case of treatment during pregnancy (37.7% versus 82.3%). Interestingly, the effect of treatment was shown to be duration dependent, especially for IgM detection, when the treatment course exceeded 8 weeks, whatever the therapy. The sensitivity of Toxoplasma PCR in blood was also lowered by maternal treatment from 39.1% to 23.2%. These results highlight that anti-Toxoplasma therapy during pregnancy may set back biological evidence of neonatal infection at birth and underline the need for a careful serological follow-up of infants with normal workup. This is the peer-reviewed version of the article: Guegan, H.; Štajner, T.; Bobić, B.; Press, C.; Olariu, R. T.; Olson, K.; Srbljanović, J.; Montoya, J. G.; Đurković-Đaković, O.; Robert-Gangneux, F. Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate. Journal of Clinical Microbiology 2021, 59 (2). [https://doi.org/10.1128/JCM.01368-20]

Published
2021

37. 1,1,4,4-Tetracyanobutadiene-Functionalized Anthracenes: Regioselectivity of Cycloadditions in the Synthesis of Small Near-IR Dyes

Author

Ziemowit Pokladek, Anh Thy Bui, Clotilde Philippe, Olivier Mongin, Katarzyna Matczyszyn, Yann Trolez, Trevor A. Hamlin, Frédéric Paul, Loïc Lemiègre, Sabrinah Batsongo-Boulingui, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Wroclaw University of Science and Technology, Vrije Universiteit Amsterdam [Amsterdam] (VU), 17-CE07-0038-01, Agence Nationale de la Recherche, 37629XG, Campus France, ARED / SAD, Région Bretagne, ANR-17-CE07-0038,Fluotet,Tétracyanobutadiènes fluorescents : de nouveaux AIEgènes pour des applications biophysiques(2017), Theoretical Chemistry, AIMMS, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)

Subjects
Letter, Photoluminescence, [CHIM.ORGA]Chemical Sciences/Organic chemistry, 010405 organic chemistry, Chemistry, Organic Chemistry, Solid-state, Regioselectivity, Chromophore, Tetracyanoethylene, 010402 general chemistry, Photochemistry, 01 natural sciences, Biochemistry, Cycloaddition, 0104 chemical sciences, 3. Good health, chemistry.chemical_compound, Reaction sequence, Physical and Theoretical Chemistry, Absorption (chemistry), SDG 6 - Clean Water and Sanitation
Abstract

Two small 1,1,4,4-tetracyanobutadiene-functionalized chromophores were obtained by careful leverage of the regioselectivity of the cycloaddition reaction of tetracyanoethylene with anthracene-ynamide derivatives, inducing either a [2+2] or a [4+2] Diels–Alder process. DFT calculations unraveled the mechanism of the [2+2] cycloaddition-retroelectrocyclization reaction sequence with ynamides and elucidated the differing mechanisms in the two substrates. The synthesized dyes presented panchromatic absorption extending into the near-IR, and far-red/near-IR photoluminescence in the solid-state up to 1550 nm.

Published
2020

38. Experimental evolution links post-transcriptional regulation to Leishmania fitness gain

Author

Laura Piel, K. Shanmugha Rajan, Giovanni Bussotti, Hugo Varet, Rachel Legendre, Caroline Proux, Thibaut Douché, Quentin Giai-Gianetto, Thibault Chaze, Thomas Cokelaer, Barbora Vojtkova, Nadav Gordon-Bar, Tirza Doniger, Smadar Cohen-Chalamish, Praveenkumar Rengaraj, Céline Besse, Anne Boland, Jovana Sadlova, Jean-François Deleuze, Mariette Matondo, Ron Unger, Petr Volf, Shulamit Michaeli, Pascale Pescher, Gerald F. Späth, Parasitologie moléculaire et Signalisation / Molecular Parasitology and Signaling, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), The Everard and Mina Goodman Faculty of Life Sciences, Bar-Ilan University [Israël], Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Biomics (plateforme technologique), Plateforme de Protéomique / Proteomics platform, Université Paris Cité (UPCité)-Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Charles University [Prague] (CU), The Mina and Everard Goodman Faculty of Life Sciences, Centre National de Recherche en Génomique Humaine (CNRGH), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), This work was supported by the Fondation de la Recherche Médicale contract FDT201805005619 (LP), the Agence Nationale pour la Recherche Labex ‘French Alliance for Parasitology and Health Care’ contract ANR-11-LABX-0024 (GFS, LP, PP, GB) and Labex ‘Integrative Biology of Emerging Infectious Diseases’ contract ANR-10-LABX-62-IBEID, the Campus France Franco-Israeli Programme Hubert Curien Maimonide 2018 (GFS and SM), the France Génomique National infrastructure, funded as part of the « Investissements d’Avenir » program managed by the Agence Nationale pour la Recherche contract ANR-10-INBS-09 (HV, RL, CP), the EU H2020 project LeiSHield-MATI - REP-778298-1 (GS), and the ERD Funds, project CePaViP (CZ.02.1.01/0.0/0.0/ 16_019/0000759) (BV, JS, PV)., We thank the CEA-CNRGH for its contribution to the sequencing costs and all the CEA-CNRGH staff who performed sample preparation and sequencing for their excellent technical assistance, ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), and European Project: 778298,H2020,H2020-MSCA-RISE-2017,LeiSHield-MATI(2018)

Subjects
Proteomics, Gene Expression Regulation, [SDV]Life Sciences [q-bio], Virology, Immunology, Genetics, Humans, Leishmaniasis, Visceral, Parasitology, Molecular Biology, Microbiology, Genomic Instability, Leishmania donovani
Abstract

The protozoan parasite Leishmania donovani causes fatal human visceral leishmaniasis in absence of treatment. Genome instability has been recognized as a driver in Leishmania fitness gain in response to environmental change or chemotherapy. How genome instability generates beneficial phenotypes despite potential deleterious gene dosage effects is unknown. Here we address this important open question applying experimental evolution and integrative systems approaches on parasites adapting to in vitro culture. Phenotypic analyses of parasites from early and late stages of culture adaptation revealed an important fitness tradeoff, with selection for accelerated growth in promastigote culture (fitness gain) impairing infectivity (fitness costs). Comparative genomics, transcriptomics and proteomics analyses revealed a complex regulatory network associated with parasite fitness gain, with genome instability causing highly reproducible, gene dosage-independent and -dependent changes. Reduction of flagellar transcripts and increase in coding and non-coding RNAs implicated in ribosomal biogenesis and protein translation were not correlated to dosage changes of the corresponding genes, revealing a gene dosage-independent, post-transcriptional mechanism of regulation. In contrast, abundance of gene products implicated in post-transcriptional regulation itself correlated to corresponding gene dosage changes. Thus, RNA abundance during parasite adaptation is controled by direct and indirect gene dosage changes. We correlated differential expression of small nucleolar RNAs (snoRNAs) with changes in rRNA modification, providing first evidence that Leishmania fitness gain in culture may be controlled by post-transcriptional and epitranscriptomic regulation. Our findings propose a novel model for Leishmania fitness gain in culture, where differential regulation of mRNA stability and the generation of modified ribosomes may potentially filter deleterious from beneficial gene dosage effects and provide proteomic robustness to genetically heterogenous, adapting parasite populations. This model challenges the current, genome-centric approach to Leishmania epidemiology and identifies the Leishmania transcriptome and non-coding small RNome as potential novel sources for the discovery of biomarkers that may be associated with parasite phenotypic adaptation in clinical settings.

Published
2022

39. Autotransporters Drive Biofilm Formation and Autoaggregation in the Diderm Firmicute Veillonella parvula

Author

Jean-Marc Ghigo, Nathalie Béchon, Emilie Bierque, Najwa Taib, Christophe Beloin, Jerzy Witwinowski, Laurence Ma, Simonetta Gribaldo, Thomas Cokelaer, Alicia Jiménez-Fernández, Génétique des Biofilms - Genetics of Biofilms, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Université Sorbonne Paris Cité (USPC), Biologie Evolutive de la Cellule Microbienne - Evolutionary Biology of the Microbial Cell, Collège doctoral [Sorbonne universités], Sorbonne Université (SU), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Biomics (plateforme technologique), Institut Pasteur [Paris], French National Research Agency (ANR) (Fir-OM ANR-16-CE12-0010), from the Institut Pasteur 'Programmes Transversaux de Recherche' (PTR 39-16), from the French government's Investissement d'Avenir Program, Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant n°ANR-10-LABX-62-IBEID) and from the Fondation pour la Recherche Médicale (grant DEQ20180339185). N.B. was supported by a MENESR (Ministère Français de l’Education Nationale, de l’Enseignement Supérieur et de la Recherche) fellowship. A.J.F. was supported by a PRESTIGE program from Campus France., ANR-16-CE12-0010,Fir-OM,Firmicutes avec une membrane externe: vers des nouveaux modeles d'étude de la transition monodermes/didermes(2016), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Collège Doctoral, and Institut Pasteur [Paris] (IP)

Subjects
Type V Secretion Systems, Firmicutes, Veillonella, Microbiology, Bacterial Adhesion, Veillonella parvula, Clostridia, 03 medical and health sciences, stomatognathic system, Spotlight, Adhesins, Bacterial, Molecular Biology, 030304 developmental biology, 0303 health sciences, Negativicutes, biology, diderm firmicutes, 030306 microbiology, autotransporter proteins, Biofilm, adhesins, biology.organism_classification, Bacterial adhesin, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biofilms, Proteobacteria, Bacterial outer membrane, Bacteria, Research Article, Autotransporters
Abstract

Veillonella parvula is an anaerobic commensal and opportunistic pathogen whose ability to adhere to surfaces or other bacteria and form biofilms is critical for it to inhabit complex human microbial communities such as the gut and oral microbiota. Although the adhesive capacity of V. parvula has been previously described, very little is known about the underlying molecular mechanisms due to a lack of genetically amenable Veillonella strains. In this study, we took advantage of a naturally transformable V. parvula isolate and newly adapted genetic tools to identify surface-exposed adhesins called autotransporters as the main molecular determinants of adhesion in this bacterium. This work therefore provides new insights on an important aspect of the V. parvula lifestyle, opening new possibilities for mechanistic studies of the contribution of biofilm formation to the biology of this major commensal of the oral-digestive tract., The Negativicutes are a clade of the Firmicutes that have retained the ancestral diderm character and possess an outer membrane. One of the best studied Negativicutes, Veillonella parvula, is an anaerobic commensal and opportunistic pathogen inhabiting complex human microbial communities, including the gut and the dental plaque microbiota. Whereas the adhesion and biofilm capacities of V. parvula are expected to be crucial for its maintenance and development in these environments, studies of V. parvula adhesion have been hindered by the lack of efficient genetic tools to perform functional analyses in this bacterium. Here, we took advantage of a recently described naturally transformable V. parvula isolate, SKV38, and adapted tools developed for the closely related Clostridia spp. to perform random transposon and targeted mutagenesis to identify V. parvula genes involved in biofilm formation. We show that type V secreted autotransporters, typically found in diderm bacteria, are the main determinants of V. parvula autoaggregation and biofilm formation and compete with each other for binding either to cells or to surfaces, with strong consequences for V. parvula biofilm formation capacity. The identified trimeric autotransporters have an original structure compared to classical autotransporters identified in Proteobacteria, with an additional C-terminal domain. We also show that inactivation of the gene coding for a poorly characterized metal-dependent phosphohydrolase HD domain protein conserved in the Firmicutes and their closely related diderm phyla inhibits autotransporter-mediated biofilm formation. This study paves the way for further molecular characterization of V. parvula interactions with other bacteria and the host within complex microbiota environments. IMPORTANCE Veillonella parvula is an anaerobic commensal and opportunistic pathogen whose ability to adhere to surfaces or other bacteria and form biofilms is critical for it to inhabit complex human microbial communities such as the gut and oral microbiota. Although the adhesive capacity of V. parvula has been previously described, very little is known about the underlying molecular mechanisms due to a lack of genetically amenable Veillonella strains. In this study, we took advantage of a naturally transformable V. parvula isolate and newly adapted genetic tools to identify surface-exposed adhesins called autotransporters as the main molecular determinants of adhesion in this bacterium. This work therefore provides new insights on an important aspect of the V. parvula lifestyle, opening new possibilities for mechanistic studies of the contribution of biofilm formation to the biology of this major commensal of the oral-digestive tract.

Published
2020

40. Activating both Halogen and Chalcogen Bonding Interactions in Cation Radical Salts of Iodinated Tetrathiafulavalene Derivatives

Author

Frédéric Barrière, Maxime Beau, Ie-Rang Jeon, Marc Fourmigué, Olivier Jeannin, Sunhee Lee, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Seoul Women's University, French National Research Agency. Grant Number: ANR 17-ERC3-0003, Campus France. Grant Number: PHC STAR 41595RK, Rennes Metropole, National Research Foundation of Korea. Grant Number: NRF-2018 K1 A3A1 A21043478, Dpt. Org. Polym. Mater., TITech, Japan. Grant Number: 2020-A0080805032, GENCI, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Halogen bond, 010405 organic chemistry, Chemistry, Halide, General Chemistry, 010402 general chemistry, Crystal engineering, 01 natural sciences, Magnetic susceptibility, 0104 chemical sciences, Ion, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Crystallography, Chalcogen, symbols.namesake, tetrathiafulvalenes, crystal engineering, halogen bonding, Halogen, symbols, chalcogen bonding, Van der Waals radius, σ-hole interactions
Abstract

International audience; Halogen bonding (XB) interactions are investigated in cation radical salts of bis(methylthio)-5,5'-diiodotetrathiafulvalene (1). Electrocrystallization of 1 in the presence of Bu NCl affords a 1 1 salt formulated as (E-1)Cl. Particularly strong I⋅⋅⋅Cl XB interactions are observed around the Cl anion with the distances at 78 % the sum of the van der Waals radii, a consequence of the XB charge activation in the cation radical. Moreover, the Cl environment is complemented by two extra S⋅⋅⋅Cl chalcogen bonding (ChB) interactions, an original feature among reported halide salts of TTF derivatives. Electrostatic potential calculations on the cation radical further demonstrate the efficient activation of the S atoms of the 1,3-dithiole rings (V =87.2 kcal/mol), as strong as with the iodine atoms (V =87.9 kcal/mol). The radical cations form weakly dimerized stacks, as confirmed by the variable-temperature magnetic susceptibility and the weak conductivity (4.8×10 S cm ).

Published
2020

41. Thin-film modeling of resting and moving active droplets

Author

Sarah Trinschek, Karin John, Uwe Thiele, Fenna Stegemerten, DYnamique des Fluides COmplexes et Morphogénèse [Grenoble] (DYFCOM-LIPhy ), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), and Campus France (PRO- COPE 2020 Grant No. 57511756), Studienstiftung des deutschen Volkes, doctoral school 'Active living fluids' funded by the German French University (Grant No. CDFA-01-14), and the DAAD

Subjects
Materials science, Drop (liquid), Surface force, Contact line, Fluid Dynamics (physics.flu-dyn), FOS: Physical sciences, Physics - Fluid Dynamics, Polarization (waves), 01 natural sciences, 010305 fluids & plasmas, Physics::Fluid Dynamics, Biological Physics (physics.bio-ph), Chemical physics, Free surface, 0103 physical sciences, Polar, Physics - Biological Physics, Wetting, Thin film, 010306 general physics, [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
Abstract

We propose a generic model for thin films and shallow drops of a polar active liquid that have a free surface and are in contact with a solid substrate. The model couples evolution equations for the film height and the local polarization profile in the form of a gradient dynamics supplemented with active stresses and fluxes. A wetting energy for a partially wetting liquid is incorporated allowing for motion of the liquid-solid-gas contact line. This gives a consistent basis for the description of drops of dense bacterial suspensions or compact aggregates of living cells on solid substrates. As example, we analyze the dynamics of two-dimensional active drops (i.e., ridges) and demonstrate how active forces compete with passive surface forces to shape droplets and drive contact line motion. The model reproduces moving and resting states of polarized droplets: Drops containing domains of opposite polarization are stationary and evolve after long transients into drops with a uniform polarization moving actively over the substrate. In our simple two-dimensional scenario droplet motion sets in at infinitely small self-propulsion force, i.e., it does not need to overcome a critical threshold., Comment: 17 pages, 16 figures

Published
2020

42. Characterization and in vitro evaluation of a vaginal gel containing Lactobacillus crispatus for the prevention of gonorrhea

Author

H. Faye-Kette, A.A.S. Aka Any-Grah, Nicolas Huang, A. Yeo, Armand Koffi, S. Bouttier, S. Villebrun, Sandrine Geiger, Valérie Nicolas, Florence Agnely, K.C. N'Guessan Gnaman, Gilles Ponchel, Institut Galien Paris-Saclay (IGPS), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Félix Houphouët-Boigny (UFHB), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur de Côte d'Ivoire, Réseau International des Instituts Pasteur (RIIP), Ingénierie et Plateformes au Service de l'Innovation Thérapeutique (IPSIT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut de recherche et de developpement (IRD), Franco-Ivorian cooperation, Campus France, AMRUGE-CI/C2D, Universite Paris-Saclay, and Tâche, Roselyne

Subjects
medicine.drug_class, [SDV]Life Sciences [q-bio], Antibiotics, Gonorrhea, Pharmaceutical Science, 02 engineering and technology, medicine.disease_cause, 030226 pharmacology & pharmacy, Microbiology, law.invention, 03 medical and health sciences, Probiotic, 0302 clinical medicine, law, Lactobacillus, medicine, Humans, Confocal laser scanning microscopy, Lactobacillus crispatus, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Gonorrhea prevention, Gel, biology, business.industry, Probiotics, Vaginal administration, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, 021001 nanoscience & nanotechnology, medicine.disease, biology.organism_classification, 3. Good health, [SDV] Life Sciences [q-bio], [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Poloxamer 407, Vagina, Neisseria gonorrhoeae, Vaginal Creams, Foams, and Jellies, Female, Rheology, 0210 nano-technology, business, Bacteria, medicine.drug
Abstract

International audience; The increasing resistance of Neisseria gonorrhoeae to any current antibiotic treatment and the difficulties associated with the use of prevention means such as condom urge the need for alternative methods to prevent this sexually transmitted infection. In this work, a prevention strategy based on the use of a vaginal gel containing Lactobacilli was assessed in vitro. A Lactobacillus crispatus strain (ATCC 33197) was selected based on the published data on its ability to inhibit Neisseria gonorrhoeae. Its probiotic properties were first characterized. Then, a thermo-sensitive hydrogel containing 21.5% of poloxamer 407, 1% of sodium alginate and 9 log(10), CFU of Lactobacillus crispatus per gel sample (5 g) was developed. The gelation temperature and the rheological characteristics of this formulation appeared suitable for a vaginal administration. Lactobacillus crispatus was viable in the gel for six months although a large amount of the bacteria was not culturable. The ability of Lactobacillus crispatus to inhibit Neisseria gonorrhoeae was still observed with the gel. Such system, thus, appeared promising for the prevention of gonorrhea.

Published
2020

43. Linearised electrodynamics and stabilisation of a cold magnetised plasma

Author

Labrunie, Simon, Zaafrani, Ibtissem, Institut Élie Cartan de Lorraine (IECL), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Campus France Eiffel Excellence Programme for its financial support (second author), Université de Sousse, and Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)

Subjects
exponential stability Mathematics Subject Classification. 35L04, exponential stability, Mathematics Subject Classification 35L04, 35Q61, 35B35, 35B40, 37L15, 47D06, 93D20, 93D22, 76N30, evolution semigroups, Mathematics - Analysis of PDEs, strong stability, Maxwell equations, Mathematics Subject Classification : 35L04, 35Q61, 35B35, 35B40, 37L15, 47D06, 93D20, 93D22, 76N30, absorbing boundary condition, FOS: Mathematics, [MATH.MATH-AP]Mathematics [math]/Analysis of PDEs [math.AP], stabilisation, hydrodynamic models, plasma, Analysis of PDEs (math.AP)
Abstract

We consider a linearized Euler--Maxwell model for the propagation and absorption of electromagnetic waves in a magnetized plasma. We present the derivation of the model, and we show its well-posedeness, its strong and polynomial stability under suitable and fairly general assumptions, its exponential stability in the same conditions as the Maxwell system, and finally its convergence to the time-harmonic regime. No homogeneity assumption is made, and the topological and geometrical assumptions on the domain are minimal. These results appear strongly linked to the spectral properties of various matrices describing the anisotropy and other plasma properties.; Nous considérons un modèle d'Euler-Maxwell linéarisé pour la propagation et l'absorption des ondes électromagnétiques dans un plasma magnétique. Nous établissons les équations du modèle, puis nous montrons sa bonne position, sa stabilité forte et polynomiale sous des hypothèses assez générales, ainsi que la stabilité exponentielle dans les même s conditions que le système de Maxwell, et sa convergence vers le régime harmonique en temps. Aucune hypothèse d'homogénéité n'est faite, et les hypothèses topologiques et géométriques sur le domaine sont minimales. Ces résultats semblent fortement liés aux propriétés spectrales de diverses matrices décrivant l'anisotropie et d'autres propriétés du plasma.

Published
2020
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44. Structural characterization and antioxidant activity of water-soluble polysaccharides from the Tunisian brown seaweed Cystoseira compressa

Author

Cédric Delattre, Christine Gardarin, Didier Le Cerf, Faiez Hentati, Alina V. Ursu, Philippe Michaud, Slim Abdelkafi, Jacques Desbrières, Guillaume Pierre, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Tunisian Ministry of higher education and scientific research and Campus France( Eiffel scholarship program of excellence , 895151J ), Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Dihydropyridines, Antioxidant, Cystoseira, Polymers and Plastics, Alginates, DPPH, medicine.medical_treatment, 02 engineering and technology, Phaeophyta, Polysaccharide, Ferric Compounds, 01 natural sciences, Antioxidants, Ferrous, chemistry.chemical_compound, Glucuronic Acid, Antioxidant activity, Polysaccharides, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Fucoidan, Materials Chemistry, medicine, Monosaccharide, Brown seaweed, Chelation, Ferrous Compounds, chemistry.chemical_classification, Molecular Structure, biology, 010405 organic chemistry, Hexuronic Acids, Alginate, Organic Chemistry, Water, [CHIM.MATE]Chemical Sciences/Material chemistry, Seaweed, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, [CHIM.POLY]Chemical Sciences/Polymers, Solubility, chemistry, Sulfated polysaccharide, 0210 nano-technology, Nuclear chemistry
Abstract

International audience; A fucoidan (CCF) and a sodium alginate (CCSA) were extracted and purified from the Tunisian brown seaweed Cystoseira compressa. CCF was a highly sulfated heterogalactofucan composed of α-(1→3), α-(1→4)-linked L-Fucp as main backbone which could be highly branched (31.84%) at O-3 and O-4 positions of α-(1→4)-L-Fucp and α-(1→3)-L-Fucp by terminal monosaccharides and side chains such as terminal α-L-Fucp, terminal β-D-Galp, β-D-Galp-(1→3)-α-L-Fucp and β-D-Galp-(1→4)-α-L-Fucp. The ratio of α-(1→3)/α-(1→4) linkages was estimated at 3.86:1. CCSA was characterized by HPAEC-PAD, GC/MS-EI, ATR-FTIR, and 1H-NMR. The M/G ratio was M/G = 0.77, indicating that CCSA respectively contained 44% and 56% of mannuronic and guluronic acids. The values of FGG, FMM, FGM (or FMG) blocks as well as the parameter η were estimated. The two polysaccharides exhibited effective antioxidant activities by ferrous ion chelation, ferric ion reduction and DPPH radical-scavenging, outlining their potentials as natural additives.

Published
2018

45. Evolution of wet agglomerates inside inertial shear flow of dry granular materials

Author

Saeid Nezamabadi, Patrick Mutabaruka, Thanh-Trung Vo, Jean-Yves Delenne, Farhang Radjai, Danang Architecture University (DAU), Physique et Mécanique des Milieux Divisés (PMMD), Laboratoire de Mécanique et Génie Civil (LMGC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ministry of Education and Training in Vietnam, Campus France, and ANR-10-LABX-0020,NUMEV,Digital and Hardware Solutions and Modeling for the Environement and Life Sciences(2010)

Subjects
Materials science, Capillary action, Structure granulaire, Mechanics, Granular material, 01 natural sciences, 010305 fluids & plasmas, Physics::Fluid Dynamics, Shear rate, Surface tension, Shear (geology), Agglomerate, [SPI.MECA.MEMA]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Mechanics of materials [physics.class-ph], 0103 physical sciences, Simulation numérique 3D, Astrophysics::Earth and Planetary Astrophysics, [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph], 010306 general physics, Shear flow, Scaling
Abstract

International audience; We use particle dynamics simulations to investigate the evolution of a wet agglomerate inside homogeneous shear flows of dry particles. The agglomerate is modeled by introducing approximate analytical expressions of capillary and viscous forces between particles in addition to frictional contacts. During shear flow, the agglomerate may elongate, break, or be eroded by loss of its capillary bonds and primary particles. By systematically varying the shear rate and surface tension of the binding liquid, we characterize the rates of these dispersion modes. All the rates increase with increasing inertial number of the flow and decreasing cohesion index of the agglomerate. We show that the data points for each mode collapse on a master curve for a dimensionless scaling parameter that combines the inertial number and the cohesion index. The erosion rate vanishes below a cutoff value of the scaling parameter. This leads to a power-law borderline between the vanishing erosion states and erosion states in the phase space defined by the inertial number and the cohesion index.

Published
2019

46. Switchable Heteroleptic Mononuclear Iron(II) Complexes as Versatile Molecular Building Block

Author

Chérif Baldé, Nicolas Paradis, Cédric Desplanches, Guillaume Chastanet, Laboratoire de Chimie et Physique (LCPM), Université Assane SECK de Ziguinchor (UASZ), Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), the Department of Cooperation and Cultural Action Embassy of France to Dakar‐Sénégal, the University of Ziguinchor and its Department of Cooperation and Research, the Ministry of Higher Education and Research of Sénégal (Program FIRST and CNDST) and CAMPUS France. The University of Bordeaux, the Centre National de la Recherche Scientifique (CNRS), the Région Nouvelle Aquitaine., ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), and ANR-13-BS04-0002,FEMTOMAT,Etude femtoseconde rayons X et optique de la dynamique ultrarapide de photocommutation de matériaux moléculaires magnétiques(2013)

Subjects
010405 organic chemistry, Chemistry, Iron, Spin crossover, 010402 general chemistry, 01 natural sciences, 0104 chemical sciences, Ligand effects, Inorganic Chemistry, Crystallography, N ligands, Block (telecommunications), Magnetic properties, [CHIM.COOR]Chemical Sciences/Coordination chemistry
Abstract

International audience; A series of photoswitchable precursors based on [Fe(NCX)2L(L′)2], where X = S or Se, L is a bidentate ligand (phenanthroline or bipyrimidine), and L′ a monodentate ligand such as a pyridine or picoline derivative, were synthesized and their spin crossover (SCO) properties investigated in detail. The thermal and light‐induced magnetic properties of a family of [Fe(NCS)2L(py)2] [py = pyridine and L = bipyrimidine (1) or phenanthroline (phen) (2)] is reported. The [Fe(NCX)2(phen)(L′)2] compounds were further explored by changing the counteranion from NCS– to NCSe– (3) or by replacing pyridine with 4‐picoline (4) or 4‐ethylpyridine (5). Complexes 1–4 exhibit thermal and light‐induced SCO, while 5 remains in the high‐spin state. The kinetics governing the photoinduced back‐conversion was investigated for compounds 2 and 4. Complex 2 exhibits LIESST relaxation kinetics involving strongly cooperative (sigmoidal) behavior, whereas complex 4 displays a stretched exponential shape, typical for broad distributions of relaxation times. These results are discussed on the basis of the steric hindrance due to bulkiness and the electronic influence of the substituent effects on the magnetic and photomagnetic properties of mononuclear iron(II) complexes.

Published
2018

47. Ionic-to-Electronic Conductivity Crossover in CdTe–AgI–As2Te3 Glasses: An 110mAg Tracer Diffusion Study

Author

Eugene Bychkov, Mohammad Kassem, D. Le Coq, I. Alekseev, Maria Bokova, Laboratoire de Physico-Chimie de l'Atmosphère ( LPCA ), Université du Littoral Côte d'Opale-Centre National de la Recherche Scientifique ( CNRS ), Institut des Sciences Chimiques de Rennes ( ISCR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Ecole Nationale Supérieure de Chimie de Rennes-Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), ANR, Agence Nationale de la Recherche, ANR-15-ASTR-0016-01, Campus France, EC, European Commission, Laboratoire de Physico-Chimie de l'Atmosphère (LPCA), Université du Littoral Côte d'Opale (ULCO)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), ANR-15-ASTR-0016,VOIR,Verres pour l'Optique dans l'InfraRouge long(2015), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Diffusion activation energy, Chalcogenide, Diffusion, Nonmonotonic behaviors, Semiconducting cadmium telluride, Oxide, Analytical chemistry, Ionic bonding, 02 engineering and technology, Activation energy, Conductivity, Ion transports, 010402 general chemistry, [ CHIM ] Chemical Sciences, 01 natural sciences, chemistry.chemical_compound, Electric conductivity, Semiconducting tellurium compounds, Materials Chemistry, Cadmium telluride, Orders of magnitude (data), Metal ions, Tracer diffusion, Physical and Theoretical Chemistry, II-VI semiconductors, [CHIM.MATE]Chemical Sciences/Material chemistry, Silver halides, 021001 nanoscience & nanotechnology, Cadmium telluride photovoltaics, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic transport, Orders of magnitude, Electronic conductivity, chemistry, Chalcogenide glass, Glass, Semiconducting silver compounds, 0210 nano-technology
Abstract

International audience; Conductivity isotherms of (CdTe)x(AgI)0.5-x/2(As2Te3)0.5-x/2 glasses (0.0 ≤ x ≤ 0.15) reveal a nonmonotonic behavior with increasing CdTe content reminiscent of mixed cation effect in oxide and chalcogenide glasses. Nevertheless, the apparent similarity appears to be partly incorrect. Using 110mAg tracer diffusion measurements, we show that semiconducting CdTe additions produce a dual effect (i) decreasing the Ag+ ion transport by a factor of ≈ 200 with a simultaneous increase of the diffusion activation energy and (ii) increasing the electronic conductivity by 1.5 orders of magnitude. Consequently, the conductivity minimum at x = 0.05 reflects an ionic-to-electronic transport crossover; the silver-ion transport number decreases by 3 orders of magnitude with increasing x. © 2018 American Chemical Society.

Published
2018

48. Use of laterite as a sustainable catalyst for removal of fluoroquinolone antibiotics from contaminated water

Author

Aymen Amin Assadi, Khalil Hanna, Sylvain Giraudet, Tiangoua Kone, Mahamadou Kamagate, Lacina Coulibaly, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Université Nangui Abrogoua, Campus France, Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Nangui Abrogoua (UNA)

Subjects
Environmental Engineering, Goethite, Environmental remediation, Iron, Morpholines, Health, Toxicology and Mutagenesis, Laterite, 02 engineering and technology, 010501 environmental sciences, Ferric Compounds, 01 natural sciences, Redox, Catalysis, Water Purification, Adsorption, UVA irradiation, Oxidation, medicine, [CHIM]Chemical Sciences, Environmental Chemistry, 0105 earth and related environmental sciences, Minerals, Chemistry, Public Health, Environmental and Occupational Health, Sorption, Hydrogen Peroxide, General Medicine, General Chemistry, Mineralization (soil science), 021001 nanoscience & nanotechnology, Pollution, 6. Clean water, Anti-Bacterial Agents, Fluoroquinolone antibiotics, Kinetics, visual_art, Environmental chemistry, Flumequine, visual_art.visual_art_medium, 0210 nano-technology, Stability, Oxidation-Reduction, Iron Compounds, Water Pollutants, Chemical, Fluoroquinolones, medicine.drug
Abstract

International audience; Although there is a growing interest in Fenton oxidation processes based on natural catalysts, the use of laterite soil to promote sequential adsorption/oxidation treatments of fluoroquinolone antibiotics has been scarcely investigated. In this work, the ability of an african laterite containing goethite and hematite to remove flumequine (FLU), used as a representative compound of fluoroquinolone antibiotics, was evaluated under dark and UVA irradiation. Batch experiments and liquid chromatography analyses showed that the presence of laterite can enhance FLU removal from heavily contaminated water through both sorption and oxidation reactions (up to 94% removal of 77 μmol L−1 of FLU and 72% of mineralization). The heterogeneous reaction rate is dominated by the rate of intrinsic surface chemical reactions including sorption and oxidation of FLU, and light-induced reduction of FeIII sites to produce FeII. Based on the probe and scavenging experiments, [rad]OH radicals were mainly involved in the heterogeneous oxidation reaction. The photo-assisted Fenton process showed a high efficiency of FLU removal even in the presence of a second fluoroquinolone antibiotic, norfloxacin (NOR), which can be co-found with FLU in affected environments. Determinations of kinetic rate constants and total organic carbon (TOC) for five sequential adsorption/oxidation cycles showed that laterite exhibited no deactivation of surface sites and an excellent catalytic stability. This cost-effective and environmentally friendly remediation technology may appear as a promising way for the removal of fluoroquinolone antibiotics from multi-contaminated waters. © 2017 Elsevier Ltd

Published
2018

49. A Seven-Year Retrospective Study on the Surveillance of Hepatitis B in Laos

Author

Philavanh Sitbounlang, Eric Deharo, François Chassagne, Sengaloun Soundala, Phimpha Paboriboune, Stéphane Bertani, François-Xavier Babin, Thomas Vial, Davone Sengmanothong, Pascal Pineau, Paul Dény, Nicolas Steenkeste, Center of Infectiology Lao-Christophe Mérieux [Vientiane] (CILM), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut de Recherche pour le Développement (IRD), Fondation Mérieux, Université Paris 13 (UP13), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Fondation Me ́rieux for providing nancial support and to medical sta and patients. Phimpha Paboriboune was sup- ported by CILM, IRD, Campus France, and Fondation Me ́rieux. Franc ̧ois Chassagne was supported by Fonda- tion pour la Recherche Me ́dicale (FDM20140731352), Paul Sabatier University of Toulouse, and the ird Cancer Plan of the French National Alliance for Life Sciences and Health (ENV201408), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), and Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
medicine.medical_specialty, Article Subject, 03 medical and health sciences, 0302 clinical medicine, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Internal medicine, Medicine, lcsh:RC799-869, Hepatology, business.industry, Incidence (epidemiology), [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Retrospective cohort study, Hepatitis B, medicine.disease, 3. Good health, Vaccination, Laos, 030220 oncology & carcinogenesis, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, lcsh:Diseases of the digestive system. Gastroenterology, 030211 gastroenterology & hepatology, Liver function, business, Liver cancer, Viral load, Research Article, Cohort study
Abstract

Objective. Lao PDR is one of the most highly endemic countries for hepatitis B in Asia and the second country for liver cancer incidence. Therefore, the follow-up of infected individuals through predictive serological markers is of utmost importance to monitor the progression of the pathology and take the decision on treatment. Methods. A retrospective-descriptive cohort study was conducted on 3,857 HBV-infected patients. Information about infection status (viral load, VL), liver function (aminotransferases), and treatments was recorded. Results. M/F sex ratio was 1.77 for a median age of 37. Patients under 37 displayed higher VL than older ones and men had higher VL than women. Initial VL ranged from 13 IU/mL. Median aminotransferase values were 45.5 U/L for ALAT and 44 U/L for ASAT, ranging from 2,000 U/L. Men had higher aminotransferase than women. Globally 20% of patients received treatment (mainly immunostimulant and reverse-transcriptase inhibitors); 11% had high levels of VL and liver enzymes, but only 2% of them were treated. Conclusion. Public health decisions should be taken urgently to rationalise vaccination and provide fair access to early diagnosis and treatment; otherwise the burden of HBV-associated diseases will be overwhelming for Laos in the near future.

Published
2018

50. Seven-coordinated iron(<scp>ii</scp>) spin-crossover molecules: some learning from iron substitution in [FexMn1−x(L222N3O2)(CN)2]·H2O solid solutions

Author

Cédric Desplanches, Hongfeng Wang, Guillaume Chastanet, Philippe Guionneau, Chérif Baldé, Arnaud Grosjean, Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie et Physique des Matériaux, Université Assane SECK de Ziguinchor (UASZ), The Department of Cooperation and Cultural Action Embassy of France to Dakar-Sénégal, the University of Ziguinchor and its Department of Cooperation and Research, the Ministry of Higher Education and Research of Sénégal (Program FIRST) and CAMPUS France., ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), and ANR-13-BS04-0002,FEMTOMAT,Etude femtoseconde rayons X et optique de la dynamique ultrarapide de photocommutation de matériaux moléculaires magnétiques(2013)

Subjects
Materials science, 010405 organic chemistry, Coordination number, [CHIM.MATE]Chemical Sciences/Material chemistry, Crystal structure, 010402 general chemistry, 01 natural sciences, 0104 chemical sciences, Inorganic Chemistry, Metal, Crystallography, Spin crossover, visual_art, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, visual_art.visual_art_medium, Molecule, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Macrocyclic ligand, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries), ComputingMilieux_MISCELLANEOUS, Solid solution, Phase diagram
Abstract

International audience; The purpose of this work is to study the influence of the substitution of Fe(II) by Mn(II) on the spin crossover behaviour of [FexMn1−x(L222N3O2)(CN)2]·H2O solid solutions where L222N3O2 is a macrocyclic ligand = 2,13-dimethyl-6,9-dioxa-3,12,18-triazabicyclo[12.3.1]-octadeca-1(18),2,12,14,16-pentaene. The pure Fe(II) complex is known to present a change of the coordination number from 7 at high temperature to 6 at low temperature. The target of the solid solution study was to investigate the effect of metal dilution on this coordination change. We have then measured the thermal spin crossover features as well as the metastable HS state lifetime generated by rapid thermal quenching, which was probed through the determination of the T(TIESST) value. A discussion of the spin-state as a function of temperature is given based on the phase diagram of this series and based on the investigation of the crystal structure of the [Mn(L222N3O2)(CN)2]·H2O complex and its comparison with the published [Fe(L222N3O2)(CN)2]·H2O master compound.

Published
2018

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