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Additional file 6: Fig. S2. HFD and UCMS differentially modulated HC gene expression of inflammatory markers and related neurobiological processes. Relative gene expression (as compared to controls) measured by TLDA analysis in the hippocampus (HC) of unstressed (Controls) or stressed (UCMS) SD and HFD mice. Detailed analysis revealed significant impact of HFD and/or UCMS for: (A) inflammatory cytokines and markers of microglial activation (IL-1��, IL-6, CD11b, CD74); (B) enzymes from the kynurenine (KYN) pathway (KAT, KYNU); (C) enzymes from the tetrahydrobiopterin (BH4) pathway (GCH1, GFRP, SPR, DHFR, PTPS); (D) key elements of the 5-HT system (5-HT1A, 5-HT1B, 5-HT2C receptors, 5-HT transporter (5-HTT), monoamine oxidase A (MAOA)); (E) markers of glutamate system (vGLUT, GLT-1, GLAST) and (F) oxidative enzymes (NOS2, CAT). (n = 8���10 mice/group). All results are graphed as means �� SEM. *P

Additional file 2: Table S1. Detailed daily schedule of the UCMS protocol.

Additional file 7: Fig. S3. HFD and UCMS differentially modulated PFC gene expression of inflammatory markers and related neurobiological processes. Relative gene expression (as compared to controls) measured by TLDA analysis in the prefrontal cortex (PFC) of unstressed (Controls) or stressed (UCMS) SD and HFD mice. Detailed analysis revealed significant impact of HFD and/or UCMS for: (A) Markers of microglial activation (CCL2, CXCL9, CXCL1, CD86, CD74); (B) enzymes from the KYN pathway (KMO, HAAO, KAT) and the neurotoxicity/neuroprotection ratio (expression level of KMO/KAT); (C) enzymes from the BH4 pathway (GCH1, PTPS); (D) key elements of the 5-HT system (5-HTT, MAOA) and (E) oxidative enzymes (NOS2, CAT). (n = 8���10 mice/group). All results are graphed as means �� SEM. *P

Additional file 4: Table S3. Plasma levels of adipokines, corticosterone and glucose measured at the end of the experiment.

Additional file 5: Fig. S1. HFD mice displayed expected increase of circulating concentrations of inflammatory factors. Plasma levels of cytokines and chemokines analyzed at the end of the experiment in unstressed (Controls) or stressed (UCMS) SD and HFD mice. Detailed statistical analysis reported significant differences between groups for circulating levels of: (A) IL-6, (B) TNF-��, (C) IL-3, (D) IL-5, (E) IL-10, (F) IL-13, (G) MIG or CXCL9, (H) IP10 or CXCL10, (I) KC or CXCL1, (J) MCP-1 or CCL2, (K) MIP-1�� or CCL4, (L) G-CSF and (M) CCL11. n = 8���15 mice/group. All results are graphed as means �� SEM. **P

Additional file 1. Supplementary methods providing detailed descriptions of the UCMS protocol, behavioral testing procedures, multiplex and TLDA assays and Z-scores calculation.