Search

Your search keyword '"Cassatella MA"' showing total 5 results
Start Over Author "Cassatella MA" Language undetermined
5 results on '"Cassatella MA"'

5. Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils

Author

Maili Zimmermann, Monica Castellucci, Francisco Bianchetto Aguilera, Marco A. Cassatella, Nicola Tamassia, Marzia Rossato, Gioacchino Natoli, Renato Ostuni, Flavia Bazzoni, Sara Costa, Giampiero Girolomoni, Claudio Lunardi, Zimmermann, M, Aguilera, Fb, Castellucci, M, Rossato, M, Costa, S, Lunardi, C, Ostuni, R, Girolomoni, G, Natoli, G, Bazzoni, F, Tamassia, N, and Cassatella, Ma.

Subjects
Neutrophils, General Physics and Astronomy, Chromatin remodelling, Ligands, Models, Biological, General Biochemistry, Genetics and Molecular Biology, Neutrophil Activation, Histones, Transcription (biology), Granulocyte Colony-Stimulating Factor, Interleukin-12 Receptor beta 1 Subunit, Animals, Humans, RNA, Messenger, Interleukin 6, Autocrine signalling, Enhancer, Promoter Regions, Genetic, Adaptor Proteins, Signal Transducing, Inflammation, IL-6, Multidisciplinary, biology, Interleukin-6, Tumor Necrosis Factor-alpha, Toll-Like Receptors, Imidazoles, Signal transducing adaptor protein, Nuclear Proteins, General Chemistry, TLR8, Chromatin remodelling, TNF-alpha, IL-6, neutrophils, Chromatin Assembly and Disassembly, Mice, Inbred C57BL, Autocrine Communication, Interleukin 1 Receptor Antagonist Protein, Enhancer Elements, Genetic, Genetic Loci, TLR4, biology.protein, Cancer research, Tumor necrosis factor alpha, I-kappa B Proteins, Peritoneum, Protein Processing, Post-Translational, TNF-alpha, Protein Binding, Transcription Factors
Abstract

Controversy currently exists about the ability of human neutrophils to produce IL-6. Here, we show that the chromatin organization of the IL-6 genomic locus in human neutrophils is constitutively kept in an inactive configuration. However, we also show that upon exposure to stimuli that trigger chromatin remodelling at the IL-6 locus, such as ligands for TLR8 or, less efficiently, TLR4, highly purified neutrophils express and secrete IL-6. In TLR8-activated neutrophils, but not monocytes, IL-6 expression is preceded by the induction of a latent enhancer located 14 kb upstream of the IL-6 transcriptional start site. In addition, IL-6 induction is potentiated by endogenous TNFα, which prolongs the synthesis of the IκBζ co-activator and sustains C/EBPβ recruitment and histone acetylation at IL-6 regulatory regions. Altogether, these data clarify controversial literature on the ability of human neutrophils to generate IL-6 and uncover chromatin-dependent layers of regulation of IL-6 in these cells.

Published
2015

Catalog

Books, media, physical & digital resources
See catalog results