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1. Pros-IT CNR: an Italian prostate cancer monitoring project

Author

Noale, Marianna, Maggi, Stefania, Artibani, Walter, Bassi, Pier Francesco, Bertoni, Filippo, Bracarda, Sergio, Conti, Giario Natale, Corvo', RENZO GIACINTO, Gacci, Mauro, Graziotti, Pierpaolo, Magrini, Stefano Maria, Maurizi Enrici, Riccardo, Mirone, Vincenzo, Montironi, Rodolfo, Muto, Giovanni, Pecoraro, Stefano, Porreca, Angelo, Ricardi, Umberto, Tubaro, Andrea, Zagonel, Vittorina, Zattoni, Filiberto, Crepaldi, Gaetano, Corvò, Renzo, Alitto, Anna Rita, Ambrosi, Enrica, Antonelli, Alessandro, Aristei, Cynthia, Barbieri, Michele, Bardari, Franco, Bardoscia, Lilia, Barra, Salvina, Bartoncini, Sara, Basso, Umberto, Becherini, Carlotta, Bellavita, Rita, Bergamaschi, Franco, Berlingheri, Stefania, Berruti, Alfredo, Borghesi, Marco, Bortolus, Roberto, Borzillo, Valentina, Bosetti, Davide, Bove, Giuseppe, Bove, Pierluigi, Brausi, Maurizio, Bruni, Alessio, Bruno, Giorgio, Brunocilla, Eugenio, Buffoli, Alberto, BUGLIONE DI MONALE E BASTIA, Michela, Buttigliero, Consuelo, Cacciamani, Giovanni, Caldiroli, Michela, Cardo, Giuseppe, Carmignani, Giorgio, Carrieri, Giuseppe, Castelli, Emanuele, Castrezzati, Elisabetta, Catalano, Gianpiero, Cattarino, Susanna, Catucci, Francesco, Cavallini Francolini, Dario, Ceccarini, Ofelia, Celia, Antonio, Chiancone, Francesco, Chini, Tommaso, Cianci, Claudia, Cisternino, Antonio, Collura, Devis, Corbella, Franco, Corinti, Matteo, Corsi, Paolo, Cortese, Fiorenza, Corti, Luigi, de Nunzio, Cosimo, Cristiano, Olga, D'Angelillo, Rolando, Da Pozzo, Luigi, D'Agostino, Daniele, D’Andrea, David, Dandrea, Matteo, De Angelis, Michele, De Cobelli, Ottavio, De Concilio, Bernardino, De Lisa, Antonello, De Luca, Stefano, De Stefani, Agostina, Deantoni, Chiara Lucrezia, Degli Esposti, Claudio, Destito, Anna, Detti, Beatrice, Di Muzio, Nadia, Di Stasio, Andrea, Di Stefano, Calogero, Di Trapani, Danilo, Difino, Giuseppe, Falivene, Sara, Farullo, Giuseppe, Fedelini, Paolo, Ferrari, Ilaria, Ferrau, Francesco, Ferro, Matteo, Fodor, Andrei, Fontana, Francesco, Francesca, Francesco, Giulio, Francolini, Frata, Paolo, Frezza, Giovanni, Gabriele, Pietro, Galeandro, Maria, Garibaldi, Elisabetta, Gennari, Pietro, Gentilucci, Alessandro, Giacobbe, Alessandro, Giussani, Laura, Giusti, Giuseppe, Gontero, Paolo, Guarneri, Alessia, Guida, Cesare, Gurioli, Alberto, Huqi, Dorijan, Imbimbo, Ciro, Ingrosso, Gianluca, Iotti, Cinzia, Italia, Corrado, La Mattina, Pierdaniele, Lamanna, Enza, Lastrucci, Luciana, Lazzari, Grazia, Liberale, Fabiola, Liguori, Giovanni, Lisi, Roberto, Lohr, Frank, Lombardo, Riccardo, Lovisolo, Jon, Ludovico, Giuseppe Mario, Macchione, Nicola, Maggio, Francesca, Malizia, Michele, Manasse, Gianluca, Mandoliti, Giovanni, Mantini, Giovanna, Marafioti, Luigi, Marciello, Luisa, Marconi, Alberto Mario, Martillotta, Antonietta, Marzano, Salvino, Masciullo, Stefano, Maso, Gloria, Massenzo, Adele, Mazzeo, Ercole, Mearini, Luigi, Medoro, Serena, Molè, Rosa, Monesi, Giorgio, Montanari, Emanuele, Montefiore, Franco, Montesi, Giampaolo, Morgia, Giuseppe, Moro, Gregorio, Muscas, Giorgio, Musio, Daniela, Muto, Paolo, Muzzonigro, Giovanni, Napodano, Giorgio, Negro, Carlo Luigi Augusto, Nidini, Mattia, Ntreta, Maria, Orsatti, Marco, Palazzolo, Carmela, Palumbo, Isabella, Parisi, Alessandro, Parma, Paolo, Pavan, Nicola, Pericolini, Martina, Pinto, Francesco, Pistone, Antonio, Pizzuti, Valerio, Platania, Angelo, Polli, Caterina, Pomara, Giorgio, Ponti, Elisabetta, Porcaro, Antonio Benito, Porpiglia, Francesco, Pugliese, Dario, Pycha, Armin, Raguso, Giuseppe, Rampini, Andrea, Randone, Donato Franco, Roscigno, Marco, Ruggieri, Maria Paola, Ruoppo, Giuseppe, Sanseverino, Roberto, Santacaterina, Anna, Santarsieri, Michele, Santoni, Riccardo, Scagliarini, Sarah, Scagliotti, Giorgio Vittorio, Scanzi, Mauro, Scarcia, Marcello, Schiavina, Riccardo, Sciarra, Alessandro, Sciorio, Carmine, Scolaro, Tindaro, Scuzzarella, Salvatore, Selvaggio, Oscar, Serao, Armando, Serni, Sergio, Signor, Marco Andrea, Silvani, Mauro, Silvano, Giovanni, Silvestris, Franco, Simeone, Claudio, Simone, Valeria, Spagnoletti, Girolamo, Spinelli, Matteo Giulio, Squillace, Luigi, Tombolini, Vincenzo, Toninelli, Mariastella, Triggiani, Luca, Trinchieri, Alberto, Trodella, Luca Eolo, Trodella, Lucio, Trombetta, Carlo, Tronnolone, Lidia, Tucci, Marcello, Urzì, Daniele, Valdagni, Riccardo, Valeriani, Maurizio, Vanoli, Maurizio, Vitali, Elisabetta, Zaramella, Stefano, Zeccolini, Guglielmo, Zini, Giampaolo, Marianna Noale, Stefania Maggi, Walter Artibani, Pier Francesco Bassi, Filippo Bertoni, Sergio Bracarda, Giario Natale Conti, Renzo Corvò, Mauro Gacci, Pierpaolo Graziotti, Stefano Maria Magrini, Riccardo Maurizi Enrici, Vincenzo Mirone, Rodolfo Montironi, Giovanni Muto, Stefano Pecoraro, Angelo Porreca, Umberto Ricardi, Andrea Tubaro, Vittorina Zagonel, Filiberto Zattoni, Gaetano Crepaldi, the Pros-IT CNR study group: [.., Marco Borghesi, Eugenio Brunocilla, Riccardo Schiavina, ], Noale, M, Maggi, S, Artibani, W, Bassi, P, Bertoni, F, Bracarda, S, Conti, G, Corvò, R, Gacci, M, Graziotti, P, Magrini, S, Maurizi Enrici, R, Mirone, V, Da Pozzo, L, and Pros-IT CNR study, G, Noale, Marianna, Maggi, Stefania, Artibani, Walter, Bassi, Pier Francesco, Bertoni, Filippo, Bracarda, Sergio, Conti, Giario Natale, Corvò, Renzo, Gacci, Mauro, Graziotti, Pierpaolo, Magrini, Stefano Maria, Maurizi Enrici, Riccardo, Mirone, Vincenzo, Montironi, Rodolfo, Muto, Giovanni, Pecoraro, Stefano, Porreca, Angelo, Ricardi, Umberto, Tubaro, Andrea, Zagonel, Vittorina, Zattoni, Filiberto, Crepaldi, Gaetano, Alitto, Anna Rita, Ambrosi, Enrica, Antonelli, Alessandro, Aristei, Cynthia, Barbieri, Michele, Bardari, Franco, Bardoscia, Lilia, Barra, Salvina, Bartoncini, Sara, Basso, Umberto, Becherini, Carlotta, Bellavita, Rita, Bergamaschi, Franco, Berlingheri, Stefania, Berruti, Alfredo, Borghesi, Marco, Bortolus, Roberto, Borzillo, Valentina, Bosetti, Davide, Bove, Giuseppe, Bove, Pierluigi, Brausi, Maurizio, Bruni, Alessio, Bruno, Giorgio, Brunocilla, Eugenio, Buffoli, Alberto, Buglione, Michela, Buttigliero, Consuelo, Cacciamani, Giovanni, Caldiroli, Michela, Cardo, Giuseppe, Carmignani, Giorgio, Carrieri, Giuseppe, Castelli, Emanuele, Castrezzati, Elisabetta, Catalano, Gianpiero, Cattarino, Susanna, Catucci, Francesco, Cavallini Francolini, Dario, Ceccarini, Ofelia, Celia, Antonio, Chiancone, Francesco, Chini, Tommaso, Cianci, Claudia, Cisternino, Antonio, Collura, Devi, Corbella, Franco, Corinti, Matteo, Corsi, Paolo, Cortese, Fiorenza, Corti, Luigi, de Nunzio, Cosimo, Cristiano, Olga, D’Angelillo, Rolando, Da Pozzo, Luigi, D’Agostino, Daniele, D’Andrea, David, Dandrea, Matteo, De Angelis, Michele, De Cobelli, Ottavio, De Concilio, Bernardino, De Lisa, Antonello, De Luca, Stefano, De Stefani, Agostina, Deantoni, Chiara Lucrezia, Degli Esposti, Claudio, Destito, Anna, Detti, Beatrice, Di Muzio, Nadia, Di Stasio, Andrea, Di Stefano, Calogero, Di Trapani, Danilo, Difino, Giuseppe, Falivene, Sara, Farullo, Giuseppe, Fedelini, Paolo, Ferrari, Ilaria, Ferrau, Francesco, Ferro, Matteo, Fodor, Andrei, Fontana, Francesco, Francesca, Francesco, Giulio, Francolini, Frata, Paolo, Frezza, Giovanni, Gabriele, Pietro, Galeandro, Maria, Garibaldi, Elisabetta, Gennari, Pietro, Gentilucci, Alessandro, Giacobbe, Alessandro, Giussani, Laura, Giusti, Giuseppe, Gontero, Paolo, Guarneri, Alessia, Guida, Cesare, Gurioli, Alberto, Huqi, Dorijan, Imbimbo, Ciro, Ingrosso, Gianluca, Iotti, Cinzia, Italia, Corrado, La Mattina, Pierdaniele, Lamanna, Enza, Lastrucci, Luciana, Lazzari, Grazia, Liberale, Fabiola, Liguori, Giovanni, Lisi, Roberto, Lohr, Frank, Lombardo, Riccardo, Lovisolo, Jon, Ludovico, Giuseppe Mario, Macchione, Nicola, Maggio, Francesca, Malizia, Michele, Manasse, Gianluca, Mandoliti, Giovanni, Mantini, Giovanna, Marafioti, Luigi, Marciello, Luisa, Marconi, Alberto Mario, Martillotta, Antonietta, Marzano, Salvino, Masciullo, Stefano, Maso, Gloria, Massenzo, Adele, Mazzeo, Ercole, Mearini, Luigi, Medoro, Serena, Molè, Rosa, Monesi, Giorgio, Montanari, Emanuele, Montefiore, Franco, Montesi, Giampaolo, Morgia, Giuseppe, Moro, Gregorio, Muscas, Giorgio, Musio, Daniela, Muto, Paolo, Muzzonigro, Giovanni, Napodano, Giorgio, Negro, Carlo Luigi Augusto, Nidini, Mattia, Ntreta, Maria, Orsatti, Marco, Palazzolo, Carmela, Palumbo, Isabella, Parisi, Alessandro, Parma, Paolo, Pavan, Nicola, Pericolini, Martina, Pinto, Francesco, Pistone, Antonio, Pizzuti, Valerio, Platania, Angelo, Polli, Caterina, Pomara, Giorgio, Ponti, Elisabetta, Porcaro, Antonio Benito, Porpiglia, Francesco, Pugliese, Dario, Pycha, Armin, Raguso, Giuseppe, Rampini, Andrea, Randone, Donato Franco, Roscigno, Marco, Ruggieri, Maria Paola, Ruoppo, Giuseppe, Sanseverino, Roberto, Santacaterina, Anna, Santarsieri, Michele, Santoni, Riccardo, Scagliarini, Sarah, Scagliotti, Giorgio Vittorio, Scanzi, Mauro, Scarcia, Marcello, Schiavina, Riccardo, Sciarra, Alessandro, Sciorio, Carmine, Scolaro, Tindaro, Scuzzarella, Salvatore, Selvaggio, Oscar, Serao, Armando, Serni, Sergio, Signor, Marco Andrea, Silvani, Mauro, Silvano, Giovanni, Silvestris, Franco, Simeone, Claudio, Simone, Valeria, Spagnoletti, Girolamo, Spinelli, Matteo Giulio, Squillace, Luigi, Tombolini, Vincenzo, Toninelli, Mariastella, Triggiani, Luca, Trinchieri, Alberto, Trodella, Luca Eolo, Trodella, Lucio, Trombetta, Carlo, Tronnolone, Lidia, Tucci, Marcello, Urzì, Daniele, Valdagni, Riccardo, Valeriani, Maurizio, Vanoli, Maurizio, Vitali, Elisabetta, Zaramella, Stefano, Zeccolini, Guglielmo, Zini, Giampaolo, Noale, M., Maggi, S., Artibani, W., Bassi, P. F., Bertoni, F., Bracarda, S., Conti, G. N., Corvo, R., Gacci, M., Graziotti, P., Magrini, S. M., Maurizi Enrici, R., Mirone, V., Montironi, R., Muto, G., Pecoraro, S., Porreca, A., Ricardi, U., Tubaro, A., Zagonel, V., Zattoni, F., Crepaldi, G., Alitto, A. R., Ambrosi, E., Antonelli, A., Aristei, C., Barbieri, M., Bardari, F., Bardoscia, L., Barra, S., Bartoncini, S., Basso, U., Becherini, C., Bellavita, R., Bergamaschi, F., Berlingheri, S., Berruti, A., Borghesi, M., Bortolus, R., Borzillo, V., Bosetti, D., Bove, G., Bove, P., Brausi, M., Bruni, A., Bruno, G., Brunocilla, E., Buffoli, A., Buglione, M., Buttigliero, C., Cacciamani, G., Caldiroli, M., Cardo, G., Carmignani, G., Carrieri, G., Castelli, E., Castrezzati, E., Catalano, G., Cattarino, S., Catucci, F., Cavallini Francolini, D., Ceccarini, O., Celia, A., Chiancone, F., Chini, T., Cianci, C., Cisternino, A., Collura, D., Corbella, F., Corinti, M., Corsi, P., Cortese, F., Corti, L., de Nunzio, C., Cristiano, O., D'Angelillo, R., Da Pozzo, L., D'Agostino, D., D'Andrea, D., Dandrea, M., De Angelis, M., De Cobelli, O., De Concilio, B., De Lisa, A., De Luca, S., De Stefani, A., Deantoni, C. L., Degli Esposti, C., Destito, A., Detti, B., Di Muzio, N., Di Stasio, A., Di Stefano, C., Di Trapani, D., Difino, G., Falivene, S., Farullo, G., Fedelini, P., Ferrari, I., Ferrau, F., Ferro, M., Fodor, A., Fontana, F., Francesca, F., Giulio, F., Frata, P., Frezza, G., Gabriele, P., Galeandro, M., Garibaldi, E., Gennari, P., Gentilucci, A., Giacobbe, A., Giussani, L., Giusti, G., Gontero, P., Guarneri, A., Guida, C., Gurioli, A., Huqi, D., Imbimbo, C., Ingrosso, G., Iotti, C., Italia, C., La Mattina, P., Lamanna, E., Lastrucci, L., Lazzari, G., Liberale, F., Liguori, G., Lisi, R., Lohr, F., Lombardo, R., Lovisolo, J., Ludovico, G. M., Macchione, N., Maggio, F., Malizia, M., Manasse, G., Mandoliti, G., Mantini, G., Marafioti, L., Marciello, L., Marconi, A. M., Martillotta, A., Marzano, S., Masciullo, S., Maso, G., Massenzo, A., Mazzeo, E., Mearini, L., Medoro, S., Mole, R., Monesi, G., Montanari, E., Montefiore, F., Montesi, G., Morgia, G., Moro, G., Muscas, G., Musio, D., Muto, P., Muzzonigro, G., Napodano, G., Negro, C. L. A., Nidini, M., Ntreta, M., Orsatti, M., Palazzolo, C., Palumbo, I., Parisi, A., Parma, P., Pavan, N., Pericolini, M., Pinto, F., Pistone, A., Pizzuti, V., Platania, A., Polli, C., Pomara, G., Ponti, E., Porcaro, A. B., Porpiglia, F., Pugliese, D., Pycha, A., Raguso, G., Rampini, A., Randone, D. F., Roscigno, M., Ruggieri, M. P., Ruoppo, G., Sanseverino, R., Santacaterina, A., Santarsieri, M., Santoni, R., Scagliarini, S., Scagliotti, G. V., Scanzi, M., Scarcia, M., Schiavina, R., Sciarra, A., Sciorio, C., Scolaro, T., Scuzzarella, S., Selvaggio, O., Serao, A., Serni, S., Signor, M. A., Silvani, M., Silvano, G., Silvestris, F., Simeone, C., Simone, V., Spagnoletti, G., Spinelli, M. G., Squillace, L., Tombolini, V., Toninelli, M., Triggiani, L., Trinchieri, A., Trodella, L. E., Trodella, L., Trombetta, C., Tronnolone, L., Tucci, M., Urzi, D., Valdagni, R., Valeriani, M., Vanoli, M., Vitali, E., Zaramella, S., Zeccolini, G., and Zini, G.

Subjects
Italian UCLA Cancer Index, Prostate cancer, Prostate cancer treatments, Quality of life, SF-12 Scale, Adult, Aged, Biopsy, Disease Management, Health Status, Humans, Italy, Male, Middle Aged, Neoplasm Staging, Prospective Studies, Prostatic Neoplasms, Quality of Life, Aging, Geriatrics and Gerontology, medicine.medical_specialty, 03 medical and health sciences, 0302 clinical medicine, medicine, 030212 general & internal medicine, Risk factor, Disease management (health), Prospective cohort study, Settore MED/36 - DIAGNOSTICA PER IMMAGINI E RADIOTERAPIA, Cancer staging, business.industry, medicine.disease, Clinical trial, Prostate cancer, Monitoring, Survival, Outcomes, Settore MED/24, Prostate cancer treatment, 030220 oncology & carcinogenesis, Family medicine, Physical therapy, Observational study, business
Abstract

Aims: The Pros-IT CNR project aims to monitor a sample of Italian males ≥18years of age who have been diagnosed in the participating centers with incident prostate cancer, by analyzing their clinical features, treatment protocols and outcome results in relation to quality of life. Methods: Pros-IT CNR is an observational, prospective, multicenter study. The National Research Council (CNR), Neuroscience Institute, Aging Branch (Padua) is the promoting center. Ninety-seven Italian centers located throughout Italy were involved. The field study began in September 1, 2014. Subjects eligible were diagnosed with biopsy-verified prostate cancer, naïve. A sample size of 1500 patients was contemplated. A baseline assessment including anamnestic data, clinical history, risk factors, the initial diagnosis, cancer staging information and quality of life (Italian UCLA Prostate Cancer Index; SF-12 Scale) was completed. Six months after the initial diagnosis, a second assessment evaluating the patient’s health status, the treatment carried out, and the quality of life will be made. A third assessment, evaluating the treatment follow-up and the quality of life, will be made 12months after the initial diagnosis. The 4th, 5th, 6th and 7th assessments, similar to the third, will be completed 24, 36, 48 and 60months after the initial diagnosis, respectively, and will include also a Food Frequency Questionnaire and the Physical Activity Scale for the Elderly. Discussion: The study will provide information on patients’ quality of life and its variations over time in relation to the treatments received for the prostate cancer.

Published
2017

2. TheC. elegansTailless/TLX transcription factornhr-67controls neuronal identity and left/right asymmetric fate diversification

Author

Celia Antonio, Sumeet Sarin, Oliver Hobert, and Baris Tursun

Subjects
animal structures, Neurogenesis, Recombinant Fusion Proteins, Molecular Sequence Data, Receptors, Cytoplasmic and Nuclear, Cell fate determination, Genes, Reporter, Two-Hybrid System Techniques, Gene expression, medicine, Animals, Humans, Cell Lineage, Gene Regulatory Networks, Amino Acid Sequence, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Molecular Biology, Transcription factor, Research Articles, Body Patterning, Neurons, Genetics, biology, Gene Expression Regulation, Developmental, biology.organism_classification, Sensory neuron, Cell biology, Phenotype, medicine.anatomical_structure, Nuclear receptor, Homeobox, Sequence Alignment, Biomarkers, Transcription Factors, Developmental Biology
Abstract

An understanding of the molecular mechanisms of cell fate determination in the nervous system requires the elucidation of transcriptional regulatory programs that ultimately control neuron-type-specific gene expression profiles. We show here that the C. elegans Tailless/TLX-type, orphan nuclear receptor NHR-67 acts at several distinct steps to determine the identity and subsequent left/right (L/R) asymmetric subtype diversification of a class of gustatory neurons, the ASE neurons. nhr-67 controls several broad aspects of sensory neuron development and, in addition, triggers the expression of a sensory neuron-type-specific selector gene, che-1, which encodes a zinc-finger transcription factor. Subsequent to its induction of overall ASE fate, nhr-67 diversifies the fate of the two ASE neurons ASEL and ASER across the L/R axis by promoting ASER and inhibiting ASEL fate. This function is achieved through direct expression activation by nhr-67 of the Nkx6-type homeobox gene cog-1, an inducer of ASER fate, that is inhibited in ASEL through the miRNA lsy-6. Besides controlling bilateral and asymmetric aspects of ASE development, nhr-67 is also required for many other neurons of diverse lineage history and function to appropriately differentiate, illustrating the broad and diverse use of this type of transcription factor in neuronal development.

Published
2009

3. Genetic Screens forCaenorhabditis elegansMutants Defective in Left/Right Asymmetric Neuronal Fate Specification

Author

Robert J. Johnston, Richard J. Poole, M. Maggie O'Meara, Dominic Didiano, Celia Antonio, Sumeet Sarin, Oliver Hobert, Surinder Narula, Sarah Chang, and Eileen B. Flowers

Subjects
Molecular Sequence Data, Mutant, Investigations, Biology, Cell fate determination, medicine.disease_cause, Ion Channels, Animals, Genetically Modified, Genes, Reporter, Sequence Homology, Nucleic Acid, Genetics, medicine, Animals, Caenorhabditis elegans, Gene, Alleles, Genes, Helminth, Body Patterning, DNA Primers, Neurons, Mutation, Base Sequence, Cell Death, Genes, Homeobox, Chromosome Mapping, DNA, Helminth, biology.organism_classification, Complementation, Phenotype, Homeobox, Genetic screen
Abstract

We describe here the results of genetic screens for Caenorhabditis elegans mutants in which a single neuronal fate decision is inappropriately executed. In wild-type animals, the two morphologically bilaterally symmetric gustatory neurons ASE left (ASEL) and ASE right (ASER) undergo a left/right asymmetric diversification in cell fate, manifested by the differential expression of a class of putative chemoreceptors and neuropeptides. Using single cell-specific gfp reporters and screening through a total of almost 120,000 haploid genomes, we isolated 161 mutants that define at least six different classes of mutant phenotypes in which ASEL/R fate is disrupted. Each mutant phenotypic class encompasses one to nine different complementation groups. Besides many alleles of 10 previously described genes, we have identified at least 16 novel “lsy” genes (“laterally symmetric”). Among mutations in known genes, we retrieved four alleles of the miRNA lsy-6 and a gain-of-function mutation in the 3′-UTR of a target of lsy-6, the cog-1 homeobox gene. Using newly found temperature-sensitive alleles of cog-1, we determined that a bistable feedback loop controlling ASEL vs. ASER fate, of which cog-1 is a component, is only transiently required to initiate but not to maintain ASEL and ASER fate. Taken together, our mutant screens identified a broad catalog of genes whose molecular characterization is expected to provide more insight into the complex genetic architecture of a left/right asymmetric neuronal cell fate decision.

Published
2007

4. In vitro Assays for Mitotic Spindle Assembly and Function

Author

Isabelle Vernos, Rebecca Heald, and Celia Antonio

Subjects
chemistry.chemical_classification, Exonuclease, biology, Centriole, Pasteur pipette, Pipette, Sperm, Molecular biology, Cell biology, chemistry.chemical_compound, Plasmid, chemistry, biology.protein, Nucleotide, DNA
Abstract

Publisher Summary This chapter focuses on in vitro assays for mitotic spindle assembly and function. Preparation of X. laevis CSF egg extracts involves dissolving in sterile water to a final concentration of 200 units/ml. Cut off the end of a glass Pasteur pipette and fire polish it to make a wide-mouth pipette. Collect laid eggs in MMR. Frogs can also be squeezed, which often gives the highest quality eggs. For preparation of DNA beads, linearized couple plasmid DNA and fill in with nucleotides so that one ends contains biotinylated bases and the other thionucleotides to inhibit exonuclease activity and the formation of large aggregates of beads. Prepare plasmid DNA by Qiagen column purification. While the sequence of the DNA is not important, the plasmid should be more than 5 kb to effectively induce chromatin assembly. Each sperm nucleus is tightly associated with a centriole. In a very simple process, half spindles can assemble around sperm nuclei just by incubation of the sperm nuclei in the extract.

Published
2006

5. Xkid chromokinesin is required for the meiosis I to meiosis II transition in Xenopus laevis oocytes

Author

Celia Antonio, Isabelle Vernos, Stéphane Flament, Angel R. Nebreda, and Laurent Perez

Subjects
DNA Replication, Cyclin B, Xenopus, Kinesins, Apoptosis, Cell Cycle Proteins, Xenopus Proteins, Xenopus laevis, Meiosis, Chromosome Segregation, CDC2 Protein Kinase, medicine, Animals, Interkinesis, Germinal vesicle, biology, urogenital system, Meiosis II, Chromosome, Nuclear Proteins, Cell Differentiation, Cell Biology, biology.organism_classification, Oocyte, Cell biology, Protein Structure, Tertiary, DNA-Binding Proteins, medicine.anatomical_structure, biology.protein, Oocytes, Female, Oligoribonucleotides, Antisense
Abstract

Xkid chromokinesin is required for chromosome alignment on the metaphase plate of spindles formed in Xenopus laevis egg extracts. We have investigated the role of Xkid in Xenopus oocyte meiotic maturation, a progesterone-triggered process that reinitiates the meiotic cell cycle in oocytes arrested at the G2/M border of meiosis I. Here we show that Xkid starts to accumulate at the time of germinal vesicle breakdown and reaches its largest quantities at metaphase II in oocytes treated with progesterone. Both germinal vesicle breakdown and spindle assembly at meiosis I can occur normally in the absence of Xkid. But Xkid-depleted oocytes cannot reactivate Cdc2/cyclin B after meiosis I and, instead of proceeding to meiosis II, they enter an interphase-like state and undergo DNA replication. Expression of a Xkid mutant that lacks the DNA-binding domain allows Xkid-depleted oocytes to complete meiotic maturation. Our results show that Xkid has a role in the meiotic cell cycle that is independent from its role in metaphase chromosome alignment.

Published
2002

6. A novel pathway for the conversion of homocysteine to methionine in eukaryotes

Author

Helga Refsum, M. Celia Antonio, K. Abraham Abraham, and C. Marta Nunes

Subjects
RNA, Transfer, Met, Homocysteine, Methionine-tRNA Ligase, Biology, Biochemistry, chemistry.chemical_compound, Methionine, Reticulocyte, Multienzyme Complexes, medicine, Animals, Methionine synthase, Molecular Biology, chemistry.chemical_classification, Sheep, Hydrolysis, Cell Biology, Methylation, Hydrogen-Ion Concentration, Amino acid, medicine.anatomical_structure, Enzyme, chemistry, Liver, Transfer RNA, biology.protein, Rabbits, Research Article
Abstract

Activation of amino acid homocysteine was compared with that of methionine in rabbit crude liver extracts and purified multi-enzyme complex of aminoacyl-tRNA synthetases. Activation was studied by measuring the incorporation of radioactive amino acid into unlabelled trichloroacetic-acid insoluble materials in the absence of protein synthesis. Homocysteine synthetase activity was found in the crude extract and in the purified multi-enzyme complex of aminoacyl-tRNA synthetases. On a molar basis, the activation of methionine by the crude extract was five times higher than the activation of homocysteine. There was a partial loss of Hcy-tRNA synthetase activity in the purified multi-enzyme complex. Preliminary reconstitution experiments indicated a requirement for an additional factor for Hcy-tRNA synthetase activity. TLC of the amino acid released from tRNA charged with [14C]homocysteine, revealed radioactivity in homocysteine, methionine and homocysteine thiolactone, indicating a conversion of tRNA-attached homocysteine to methionine. Total tRNA was separated on a benzoylated cellulose column into a fraction enriched in initiator tRNA and a methionine-accepting, but initiator tRNA-deficient, fraction. Homocysteine-accepting activity was present only in the initiator tRNA-enriched fraction. Based on the above data we propose that homocysteine activation in reticulocyte lysates, reported previously, also occurs in liver. Activated homocysteine is attached to initiator tRNA and then converted to methionine by a methylating enzyme. In the absence of methylation, tRNA-attached homocysteine is hydrolysed to produce homocysteine thiolactone.

Published
1998

8. Prediction of significant renal function decline after open, laparoscopic, and robotic partial nephrectomy: External validation of the Martini’s nomogram on the RECORD2 project cohort

Author

Alessandro Antonelli, Andrea Mari, Alessandro Tafuri, Riccardo Tellini, Umberto Capitanio, Paolo Gontero, Antonio Andrea Grosso, Vincenzo Li Marzi, Nicola Longo, Francesco Porpiglia, Angelo Porreca, Bernardo Rocco, Claudio Simeone, Riccardo Schiavina, Luigi Schips, Salvatore Siracusano, Carlo Terrone, Vincenzo Ficarra, Marco Carini, Andrea Minervini, Vincenzo Altieri, Daniele Amparore, Walter Artibani, Fabrizio Di Maida, Francesco Berardinelli, Pierluigi Bove, Carlo Andrea Bravi, Eugenio Brunocilla, Anna Cadenar, Antonio Celia, Elisabetta Costantini, Luigi Da Pozzo, Alberto Diminutto, Mario Falsaperla, Gaetano Grosso, Luca Lambertini, Alessandro Larcher, Francesco Maiorino, Giancarlo Marra, Francesco Montorsi, Andrea Polara, Riccardo Rizzetto, Marco Roscigno, Alchiede Simonato, Carlo Trombetta, Antonelli, Alessandro, Mari, Andrea, Tafuri, Alessandro, Tellini, Riccardo, Capitanio, Umberto, Gontero, Paolo, Andrea Grosso, Antonio, Li Marzi, Vincenzo, Longo, Nicola, Porpiglia, Francesco, Porreca, Angelo, Rocco, Bernardo, Simeone, Claudio, Schiavina, Riccardo, Schips, Luigi, Siracusano, Salvatore, Terrone, Carlo, Ficarra, Vincenzo, Carini, Marco, Minervini, Andrea, Berardinelli, Francesco, Bove, Pierluigi, Andrea Bravi, Carlo, Brunocilla, Eugenio, Cadenar, Anna, Celia, Antonio, Costantini, Elisabetta, Da Pozzo, Luigi, Diminutto, Alberto, Falsaperla, Mario, Grosso, Gaetano, Lambertini, Luca, Larcher, Alessandro, Maiorino, Francesco, Marra, Giancarlo, Montorsi, Francesco, Polara, Andrea, Rizzetto, Riccardo, Roscigno, Marco, Simonato, Alchiede, Trombetta, Carlo, and Alessandro Antonelli, Andrea Mari, Alessandro Tafuri, Riccardo Tellini, Umberto Capitanio, Paolo Gontero, Antonio Andrea Grosso, Vincenzo Li Marzi, Nicola Longo, Francesco Porpiglia, Angelo Porreca, Bernardo Rocco, Claudio Simeone, Riccardo Schiavina, Luigi Schips, Salvatore Siracusano, Carlo Terrone, Vincenzo Ficarra, Marco Carini, Andrea Minervini, Vincenzo Altieri, Daniele Amparore, Walter Artibani, Fabrizio Di Maida, Francesco Berardinelli, Pierluigi Bove, Carlo Andrea Bravi, Eugenio Brunocilla, Anna Cadenar, Antonio Celia, Elisabetta Costantini, Luigi Da Pozzo, Alberto Diminutto, Mario Falsaperla, Gaetano Grosso, Luca Lambertini, Alessandro Larcher, Francesco Maiorino, Giancarlo Marra, Francesco Montorsi, Andrea Polara, Riccardo Rizzetto, Marco Roscigno, Luigi Schips, Alchiede Simonato, Carlo Trombetta

Subjects
laparoscopy, nephron-sparing surgery, renal cell carcinoma, renal function, robotics, Humans, Kidney, Nephrectomy, Nomograms, Kidney Neoplasms, Laparoscopy, Robotic Surgical Procedures, Robotics, Urology
Abstract

Objectives: Martini et al. developed a nomogram to predict significant (>25%) renal function loss after robot-assisted partial nephrectomy and identified four risk categories. We aimed to externally validate Martini’s nomogram on a large, national, multi-institutional data set including open, laparoscopic, and robot-assisted partial nephrectomy. Methods: Data of 2584 patients treated with partial nephrectomy for renal masses at 26 urological Italian centers (RECORD2 project) were collected. Renal function was assessed at baseline, on third postoperative day, and then at 6, 12, 24, and 48 months postoperatively. Multivariable models accounting for variables included in the Martini’s nomogram were applied to each approach predicting renal function loss at all the specific timeframes. Results: Multivariable models showed high area under the curve for robot-assisted partial nephrectomy at 6- and 12-month (87.3% and 83.6%) and for laparoscopic partial nephrectomy (83.2% and 75.4%), whereas area under the curves were lower in open partial nephrectomy (78.4% and 75.2%). The predictive ability of the model decreased in all the surgical approaches at 48 months from surgery. Each Martini risk group showed an increasing percentage of patients developing a significant renal function reduction in the open, laparoscopic and robot-assisted partial nephrectomy group, as well as an increased probability to develop a significant estimated glomerular filtration rate reduction in the considered time cutoffs, although the predictive ability of the classes was

Published
2022

9. Assessing Trifecta Achievement after Percutaneous Cryoablation of Small Renal Masses: Results from a Multi-Institutional Collaboration

Author

Andrea Piasentin, Francesco Claps, Tommaso Silvestri, Giacomo Rebez, Fabio Traunero, Maria Carmen Mir, Michele Rizzo, Antonio Celia, Calogero Cicero, Martina Urbani, Luca Balestreri, Lisa Pola, Fulvio Laganà, Stefano Cernic, Maria Assunta Cova, Michele Bertolotto, Carlo Trombetta, Giovanni Liguori, Nicola Pavan, Piasentin, Andrea, Claps, Francesco, Silvestri, Tommaso, Rebez, Giacomo, Traunero, Fabio, Mir, Maria Carmen, Rizzo, Michele, Celia, Antonio, Cicero, Calogero, Urbani, Martina, Balestreri, Luca, Pola, Lisa, Laganà, Fulvio, Cernic, Stefano, Cova, Maria Assunta, Bertolotto, Michele, Trombetta, Carlo, Liguori, Giovanni, and Pavan, Nicola

Subjects
Treatment Outcome, cryoablation, renal cancer, Humans, General Medicine, small renal masses, Cryosurgery, Nephrectomy, Kidney Neoplasms, Aged, Glomerular Filtration Rate, Retrospective Studies
Abstract

Background and Objectives: To assess efficacy and safety of Percutaneous Cryoablation (PCA) of small renal masses (SRMs) using Trifecta outcomes in a large cohort of patients who were not eligible for surgery. Materials and methods: All PCAs performed in four different centers between September 2009 and September 2019 were retrospectively evaluated. Patients were divided in two different groups depending on masses dimensional criteria: Group-A: diameter ≤ 25 mm and Group-B: diameter > 25 mm. Complications rates were reported and classified according to the Clavien–Dindo system. The estimate glomerular filtration rate (eGFR) was calculated before PCA and during follow-up schedule. Every patient received a Contrast Enhanced Ultrasound (CEUS) evaluation on the first postoperative day. Radiological follow-up was taken at 3, 6, and 12 months for the first year, then yearly. Radiological recurrence was defined as a contrast enhancement persistence and was reported in the study. Finally, Trifecta outcome, which included complications, RFS, and preservation of eGFR class, was calculated for every procedure at a median follow-up of 32 months. Results: The median age of the patients was 74 years. Group-A included 200 procedures while Group-B included 140. Seventy-eight patients were eligible for Trifecta evaluation. Trifecta was achieved in 69.6% of procedures in Group-A, 40.6% in Group-B (p = 0.02). We observed an increased rate of complication in Group-B (13.0% vs. 28.6; p < 0.001). However, 97.5% were

Published
2022

10. The relationship between obstructive sleep apnoea and erectile dysfunction: An underdiagnosed link? A prospective cross-sectional study

Author

Elena Cantone, Matteo Massanova, Felice Crocetto, Biagio Barone, Fabio Esposito, Davide Arcaniolo, Fabrizio Corlianò, Luigi Romano, Gaetano Motta, Antonio Celia, Cantone, E., Massanova, M., Crocetto, F., Barone, B., Esposito, F., Arcaniolo, D., Corliano, F., Romano, L., Motta, G., Celia, A., Cantone, Elena, Massanova, Matteo, Crocetto, Felice, Barone, Biagio, Esposito, Fabio, Arcaniolo, Davide, Corlianò, Fabrizio, Romano, Luigi, Motta, Gaetano, and Celia, Antonio

Subjects
Male, Sleep Apnea, Obstructive, Sleepiness, Urology, General Medicine, ED, SatO2, OSA, Oxygen, Endocrinology, Cross-Sectional Studies, Erectile Dysfunction, Obstructive sleep apnoea, Risk Factors, Humans, Prospective Studies
Abstract

This cross-sectional study aimed to investigate the prevalence and clinical characteristics of erectile dysfunction in patients with obstructive sleep apnoea. We enrolled 133 male patients with suspected obstructive sleep apnoea. Ear, nose and throat evaluation, laboratory tests, body mass index, Epworth sleepiness scale, 5-international index of erectile function, overnight ambulatory polygraphy and drug-induced sleep endoscopy patterns were assessed. Eighty patients reported obstructive sleep apnoea. 60% (n=48) reported erectile dysfunction. Statistically significant correlations were found between 5-International Index of Erectile Function and age, hypertension, diabetes, Epworth sleepiness scale, apnoea-hypopnea index score, O2 saturation-nadir, and oxygen desaturation index. Age, diabetes and O2 saturation-nadir were independent predictors of erectile function. Epworth sleepiness scale, apnoea-hypopnea index score, O2 saturation-nadir, oxygen desaturation index and albumin were higher compared to patients without erectile dysfunction. No statistically significant differences were reported for drug-induced sleep endoscopy patterns and erectile dysfunction. Patients with obstructive sleep apnoea were at significant risk of having erectile dysfunction. Males with obstructive sleep apnoea should be investigated for erectile dysfunction.

Published
2022

11. Percutaneous cryoablation for high-complexity renal masses: complications, functional and oncological outcomes

Author

Giorgio Artuso, Nicola Pavan, Antonio Celia, Luca Balestrieri, Giacomo Rebez, Paolo Umari, G. Giannarini, Carlo Trombetta, Michele Rizzo, Michele Bertolotto, Giovanni Liguori, Camilla Sachs, Andrea Piasentin, Rizzo, Michele, Piasentin, Andrea, Rebez, Giacomo, Giannarini, Gianluca, Umari, Paolo, Sachs, Camilla, Celia, Antonio, Pavan, Nicola, Balestrieri, Luca, Artuso, Giorgio, Bertolotto, Michele, Trombetta, Carlo, and Liguori, Giovanni

Subjects
Cryoablation, medicine.medical_specialty, Percutaneous, Local neoplasm recurrence, Urology, medicine.medical_treatment, Renal function, Cryosurgery, High complexity, medicine, Overall survival, Humans, Renal neoplasm, Renal cell carcinoma, Carcinoma, Renal Cell, Aged, Retrospective Studies, Percutaneous cryoablation, business.industry, Kidney Neoplasms, Surgery, Treatment Outcome, Nephrology, Median time, Charlson comorbidity index, Percutaneou, business
Abstract

Background During the past two decades cryoablation (CA) has become a therapeutic option for the management of localized cT1 renal masses in comorbid patients. We analyzed the midterm functional and oncological outcomes of CA in the treatment of cT1 renal masses which were classified as high-complexity masses according to the PADUA system. Methods A total of 299 patients underwent percutaneous CA between November 2007 and December 2018 in 4 institutions for cT1N0M0 renal masses. All patients with highcomplexity (PADUA ≥ 10) renal tumors were included. Technical failure of CA was considered an exclusion criterion. Results Inclusion criteria were met by 45 patients. Median Charlson Comorbidity Index (CCI) was 6.0 (IQR: 5.0-7.0), median age was 74 years (IQR: 64.5-79.5). Seven Clavien 1 and 1 Clavien 2 procedure-related complications were reported. Median eGFR at baseline was 64.3ml/min (IQR: 52.0 - 82.3) while at the 1-year follow-up was 61.4 ml/min (IQR: 44.0-74.5). The median follow-up was 32 months (IQR: 13.25-47.5). Local recurrences were detected in 6 patients; 3 of them underwent re-cryoablation while the others started active surveillance. Median time to recurrence was 17.5 months (IQR: 7.8-27.3). Cancer-Specific Survival and Metastasis-Free Survival were 100%. Overall survival was 86.7%. Conclusions CA proved to be a valuable therapeutic option for the management of patients with cT1 high-complexity PADUA ≥ 10 renal tumors as it provides a low rate of procedural morbidity and good preservation of renal function. However, these results are counterbalanced by a recurrence rate that appears to be higher than those reported on surgically treated patients.

Published
2021

12. Laparoscopic radical cystectomy with extracorporeal urinary diversion: an Italian single-center experience with 10-year outcomes

Author

Massimiliano Creta, Antonio Celia, Marina Di Mauro, Bernardino De Concilio, Tommaso Silvestri, Felice Crocetto, Maida Bada, Bada, Maida, De Concilio, Bernardino, Crocetto, Felice, Creta, Massimiliano, Silvestri, Tommaso, Di Mauro, Marina, and Celia, Antonio

Subjects
Carcinoma, Transitional Cell, medicine.medical_specialty, business.industry, Urology, medicine.medical_treatment, General surgery, Urinary Bladder, Urinary diversion, MEDLINE, Urinary Diversion, Cystectomy, Single Center, Extracorporeal, Treatment Outcome, Italy, Urinary Bladder Neoplasms, Nephrology, Humans, Medicine, Laparoscopy, business
Published
2020

13. PD60-10 IMPACT OF PREOPERATIVE CONTROLLING NUTRITIONAL STATUS (CONUT) SCORE ON PERIOPERATIVE MORBIDITY AND SURVIVAL OUTCOMES IN PATIENTS WITH BLADDER CANCER TREATED WITH RADICAL CYSTECTOMY: A MULTICENTER ANALYSIS

Author

Matteo Massanova, Nicola Pavan, Maria Carmen Mir Maresma, Shahrokh F. Shariat, José Rubio Briones, Francesco Soria, Miguel Ramírez-Backhaus, Carlo Trombetta, David D'Andrea, Francesco Claps, Giorgio Mazzon, Giancarlo Marra, Antonio Celia, Paolo Gontero, Fabio Traunero, M. Boltri, Giovanni Liguori, Claps, Francesco, Pavan, Nicola, Carmen Mir Maresma, Maria, Mazzon, Giorgio, Ramirez-Backhaus, Miguel, Boltri, Matteo, Soria, Francesco, Massanova, Matteo, D'Andrea, David, Traunero, Fabio, Marra, Giancarlo, Liguori, Giovanni, Gontero, Paolo, François Shariat, Shahrokh, Rubio Briones, Jose, Celia, Antonio, and Trombetta, Carlo

Subjects
medicine.medical_specialty, Bladder cancer, business.industry, Urology, medicine.medical_treatment, Nutritional status, Perioperative, medicine.disease, Surgery, Cystectomy, Medicine, In patient, business
Abstract

INTRODUCTION AND OBJECTIVE: Despite several improvements in surgical technique, radical cystectomy (RC) remains an highly morbid operation and even in contemporary series the combined surgical, medical and disease morbidity results in a low 5-years overall survival (OS). Furthermore, the age of patients undergoing RC has increased and the ability to predict perioperative morbidity is crucial in the preoperative workup of RC. The controlling nutritional status (CONUT), a score based on the serum albumin concentration, lymphocyte count and total cholesterol concentration, is a comprehensive index that could provide an immune response status and long-term nutritional effect of the host. Recently, its prognostic value has been reported in many types of malignancies but it’s never tested in bladder cancer (BC). The aim of the study is to evaluate the role of preoperative CONUT score in the assessment of both oncological outcomes and perioperative morbidity in patients underwent RC for non-metastatic BC. METHODS: We retrospectively evaluated data from 347 patients who underwent RC for BC at ve european high-volume centers between January 2002 and December 2018. Patients were divided into two groups according to the optimal cut-off value of CONUT score. Relationships of CONUT score with clinicopathological characteristics, perioperative complications (according to the Calvien-Dindo system), 30-days readmission, 90-days mortality, cancer-specific mortality (CSM), overall mortality (OM) and progression-free survival (PFS) were analyzed. RESULTS: Cut-off value to discriminate between high and low CONUT score was determined calculating the ROC curve: the area under the curve was 0.72 with an optimal cut-off of 3 points. High preoperative CONUT score was significantly correlated with older age, worse ASA score, advanced pT stage, high grade and node-positive disease, higher median length of stay and 30-days readmission (all p

Published
2020

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