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Additional file 2. Figure S1. Prolyl hydroxylase domain inhibitor induces hypoxia-inducible factor 1�� and hypoxia-inducible factor 2�� in C3H10T1/2 cells. Figure S2. SiRNA transfection specific for Hif1a and Epas1 downregulates hypoxia-induced genes. Figure S3. 10T1/2 cells exhibited higher levels of TGF-��1 signaling. Figure S4. TGF-��1 inhibits PHDi-induced HIF2�� expression through activin receptor-like kinase-5 in 10T1/2 cells. Figure S5. TGF-��1 inhibits hypoxia-induced nuclear accumulation of HIF2�� through ALK5 in 10T1/2 cells. Figure S6. TGF-��1 increases hypoxia-induced expression of Egln1, Vegfa and Slc2a1 through ALK5 in 10T1/2 cells. Figure S7. TGF-��1 does not change methylation in 5��� flanking regions of Epo and Epas1 genes in 10T1/2 cells.

Additional file 1.Table S1. Primer sequences used in quantitative polymerase chain reaction. Table S2. Primer sequences used in (quantitative) chromatin immunoprecipitation polymerase chain reaction. Table S3. Primer sequences used in methylation-specific polymerase chain reaction of Epo and Epas1 5��� flanking regions.