Search

To apply the chemiluminescence immunoassay method in clinical applications,some key technologies were investigated based on deeply analyzing the chemiluminescence immunoassay process.Firstly,three key steps about the immunoassay were indicated: filling-up,washing and measuring.Accordingly,three key technologies were summarized: accurate filling-up of microcontent liquid,quick and effective separation of immune complex,and accurate measurement of weak optical signal.In the aspect of filling-up,based on the analysis and simulation of milliliter level liquid filling-up process,the principle for optimizing relative sampling parameter was obtained.In the aspect of washing,the principle for designing the magnetic field was then obtained based on the analysis of separating immune complex process.In the aspect of measuring,the principles for designing measuring room and processing optical signal were given.After investigating the above-mentioned key technologies,chemiluminescence immunoassay analyzer Autolumis 3000 has been developed and applied in hospital.The results show that the coefficient of variance(CV) is 5% and the maximum speed is 180 time/h.This analyzer can achieve similar performance comparing with the current high-end equipment in abroad,which validates our investigation of the key technologies.