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Background Lung cancer is the leading cause of death among cancer patients worldwide. Close to 85% of lung cancer pathology types are non-small cell lung cancer (NSCLC). With advances in medicine, the survival rate of early-stage NSCLC has improved. However, its overall 5-year survival rate and recurrence rate remain high. The search for potential therapeutic targets in the development of NSCLC provides new avenues for clinical treatment. In recent years, related studies have shown that microRNA-486 (miRNA-486) can promote tumor cell proliferation, infiltration, metastasis and drug resistance, and can be used as a new molecular marker for early diagnosis, prognosis and treatment of tumors. However, the expression and prognosis of miRNA-486 in NSCLC are unclear. Therefore, this study further elucidated the relationship between miRNA-486 expression and prognosis in NSCLC through meta-analysis. In addition, the possible molecular mechanism of miRNA-486 was further analyzed by bioinformatics analysis to provide theoretical basis for the diagnosis, treatment and prognosis of NSCLC patients. Methods Wanfang, Chinese Biomedical Literature Database, Chinese National Knowledge Infrastructure, the Chongqing VIP Chinese Science and Technology Periodical Database, PubMed, Embase, and Web of Science databases were searched. The search was scheduled from the establishment of the database to August 2021. Combined hazard ratios (HRs) and 95% confidence intervals (95% CI) were used to evaluate the effect of miRNA-486 on the prognosis of NSCLC. Stata 16.0 software was used for meta-analysis. The expression of miRNA-486 in NSCLC was analyzed using the GEO database and TCGA database. Survival curves of miRNA-486 differential expression were analyzed by OncomiR. Target genes of miRNA-486 were predicted by TargetScan, miRBase, miRTarBase, starBase V2.0 and miRWalk. Target genes were obtained by the intersection of Venn diagrams. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed using the DAVID database. Results The results of this meta-analysis will be submitted to a peer-reviewed journal for publication. Conclusion This study provides high-quality evidence support for the expression of miRNA-486 and the prognosis of NSCLC. Through bioinformatics analysis, we further discussed the mechanism of miRNA-486 in NSCLC and the understanding of related pathways.

Background: The morbidity and mortality of non-small cell lung cancer (NSCLC) are high, platinum-based chemotherapy has reached the efficacy threshold, and its use is limited by drug toxicity, so there is an urgent need to find a new effective and safe treatment. Chemotherapy, antiangiogenic therapy and immunotherapy are the routine methods for the treatment of NSCLC. However, the effect of the combination of the three treatments is currently controversial. Therefore, this study uses meta-analysis to further explore the efficacy and safety of recombinant human endostatin and chemotherapy combined with PD-1 inhibitors in the treatment of NSCLC, so as to provide a reference basis for clinical decision-making. Methods: Relevant publications were searched from the clinical trials. Using computer to retrieve on Cochrane Central Register of Controlled Trials Repositories, PubMed, Embase and Web of Science databases. The retrieval time limit was from the establishment of the database to January 31, 2021. The two researchers independently carried out data extraction and literature quality evaluation on the quality and Meta-analysis of the included literature was carried out using Revman5.3 software. Results: The results of this meta-analysis will be submitted to a peer-reviewed journal for publication. Conclusion: This study will provide reliable evidence-based basis for the clinical application of recombinant human endostatin and chemotherapy combined with PD-1 inhibitors in the treatment of advanced NSCLC.

To evaluate salt tolerance and organ-specific responses of Populus deltoides × Populus euramericana cv., ‘NL895’ to salinity stress, plantlets were cultured on half-strength Murashige and Skoog medium containing 0, 50, 100, 150, or 200 mM NaCl for 15–30 d. The growth, ion homeostasis, antioxidant activities, and ultrastructural responses of the roots and leaves were assessed. Sodium chloride, at 50 and 100 mM (there were mostly no statistical differences in the two levels), induced lower Na+ contents, higher K+/Na+ ratio, and lower H2O2 and malondialdehyde (MDA) levels in roots compared to those in leaves. However, salt-stressed roots exhibited greater peroxidase (POD) and glutathione reductase (GR) activities and higher ascorbic acid (AsA) contents compared to leaves. The leaves maintained higher K+ and Ca2+ levels than the roots during NaCl treatment and partly balanced the excess Na+ to decrease Na+ toxicity. Also, leaves reduced oxidative damage by increasing the activities of the antioxidation enzymes superoxide dismutase and catalase, and by increasing AsA and glutathione (GSH) levels. Additionally, an increase in the stomatal density was observed in the NaCl-treated leaves. Overall, roots and leaves displayed different adaptive mechanisms to cope with salt stress and the roots were more tolerant to salt stress than the leaves. Coordination of Na+ and K+ transport in roots and leaves, and the organ-specificity of high POD activity in roots, and high CAT and APX activities in leaves, likely contributed to the salt tolerance of the whole plant. ‘NL895’ poplar could survive at a moderate NaCl level of 100 mM.

Summary Glyceollins, stress-induced phytoalexins from parent soya bean isoflavones, were elicited with Aspergillus oryzae. This solid-state fermentation facilitated the conversion of isoflavone glycosides into aglycones and glyceollins, which could mainly enrich in soya bean protein isolate (SPI) during protein preparation due to protein–polyphenol interactions. Glyceollin-enriched SPI exhibited less flavour volatiles, higher solubility, lower whiteness and higher antioxidant activity than unfermented SPI. Fermented SPI was more easily to be digested during in vitro pepsin–trypsin digestion. This may be attributed to partial degradation of protein, especially α′ and α subunits of β-conglycinin and acidic subunit of glycinin. The antioxidant activity of digestive products derived from fermented SPI was obviously enhanced with increasing digestion time due to simultaneous release of antioxidant peptides and glyceollins involved in the interior of protein molecule. These results suggest an effective technique for producing a nutrient-enhanced SPI as novel functional ingredients applied in food industry.

Summary In this study, we investigated the effects of dextran glycation on soya β-conglycinin self-assembly into nanofibrils at 85 °C and pH 2.0, as well as their stability at pH 2.0–10.0. Although the hydrolysis rate of β-conglycinin decreased, glycation significantly increased the structural change rate in the initial stage of nanofibril formation and the growth of nanofibril. It is suggested that the glycation of three subunits (α′, α, β) in β-conglycinin may promote fibril assembly because the extension regions of α′ and α subunits play an important role in affecting the rate of structural changes in fibril formation. At neutral pH, conjugate nanofibrils are highly dispersible and transparent and remained greater structural stability compared with mixture. The improvement stability of conjugate nanofibrils may be contributed to dextran which provides some steric hindrance to prevent the aggregation of nanofibrils; this would also facilitate the application of soya β-conglycinin nanofibrils in food industry.