1. Peripheral blood mononuclear cells contribute to myogenesis in a 3D bioengineered system of bone marrow mesenchymal stem cells and myoblasts
- Author
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Pasqualina Scala, Paola Manzo, Erwin Pavel Lamparelli, Joseph Lovecchio, Maria Camilla Ciardulli, Valentina Giudice, Carmine Selleri, Emanuele Giordano, Laura Rehak, Nicola Maffulli, Giovanna Della Porta, Scala, Pasqualina, Manzo, Paola, Lamparelli, Erwin Pavel, Lovecchio, Joseph, Ciardulli, Maria Camilla, Giudice, Valentina, Selleri, Carmine, Giordano, Emanuele, Rehak, Laura, Maffulli, Nicola, and Della Porta, Giovanna
- Subjects
Myogenesis, Tissue engineering, Mesenchymal stem cells, Peripheral blood mononuclear cells, 3D Co-culture model ,Histology ,Biomedical Engineering ,Bioengineering ,Biotechnology - Abstract
In this work, a 3D environment obtained using fibrin scaffold and two cell populations, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), and primary skeletal muscle cells (SkMs), was assembled. Peripheral blood mononuclear cells (PBMCs) fraction obtained after blood filtration with HemaTrate® filter was then added to the 3D culture system to explore their influence on myogenesis. The best cell ratio into a 3D fibrin hydrogel was 1:1 (BM-MSCs plus SkMs:PBMCs) when cultured in a perfusion bioreactor; indeed, excellent viability and myogenic event induction were observed. Myogenic genes were significantly overexpressed when cultured with PBMCs, such as MyoD1 of 118-fold at day 14 and Desmin 6-fold at day 21. Desmin and Myosin Heavy Chain were also detected at protein level by immunostaining along the culture. Moreover, the presence of PBMCs in 3D culture induced a significant downregulation of pro-inflammatory cytokine gene expression, such as IL6. This smart biomimetic environment can be an excellent tool for investigation of cellular crosstalk and PBMC influence on myogenic processes.
- Published
- 2023