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Purpose: To investigate 6-methyl-1-(3-(trifluoromethyl)phenyl)-1H-pyrazolo[4,3-c]pyridin-4(5H)-one (FMPPP) as anti-proliferative agent against prostate cancers. Methods: The FMPPP-mediated changes in cell proliferation were measured using cell counting kit-8 (CCK-8). Flow cytometry and propidium iodide staining were used for cellular DNA content determination. Proteins expression in cells was probed by western blotting assay. Results: A significant (p < 0.05) dose-dependent suppression of DU145 and PC 3 cell proliferation was observed following FMPPP treatment. FMPPP treatment at 20 μM raised DU145 cell fraction to 75.08 ± 4.87 % in G1 phase when compared to 48.32 ± 3.44 % for control. The population of PC 3 cells in G1- phase reached to 72.78 ± 5.21 % on treatment with 20 μM FMPPP compared to 49.65 ± 4.62 % in control. The FMPPP treatment of DU145 and PC-3 cells elevated LC3-II expression and suppressed SQSTM1/p62 expression. In FMPPP-treated DU145 and PC-3 cells, p-ERK1/2 level was promoted whereas mTOR and p70S6K phosphorylation significantly decreased. Exposure to U0126 (ERK pathway inhibitor) reduced FMPPP-induced increase of LC3-II expression and promotion of p-ERK1/2 level in DU145 and PC-3 cells. Conclusion: FMPPP exhibits anti-proliferative effect by increasing autophagy in prostate cancer cells. The cytotoxicity of FMPPP involves elevation of ERK1/2 phosphorylation and targeting mTOR pathway in DU145 and PC-3 cells. Therefore, FMPPP may be beneficial for the treatment of prostate cancer in patients.

Supplemental material, sj-docx-1-trr-10.1177_03611981221092722 for Procurement Benchmarks for Major Transportation Projects by Kunqi Zhang, Abdolmajid Erfani, Ousama Beydoun and Qingbin Cui in Transportation Research Record

Managing shipboard industrial fire safety during a depot or intermediate level maintenance availability on a commissioned naval vessel can be viewed as a complex system bounded by the defense acquisition system. Sociotechnical factors define the hazard and associated risk and risk management practices, and this complex system governs the resulting level of fire safety. Poor industrial fire safety practices during naval ship maintenance availabilities can directly impact project cost and schedule. If a fire is severe enough, these effects can trickle throughout the ship class's maintenance program, adversely impacting fleet readiness and operations. Traditional viewpoints on fire safety prescriptively regulate the fire hazard. Rote compliance with this type of requirement does not provide clear mechanisms for measuring safety performance, resulting in uneven risk management. This paper presents a safety management system (SMS) framework for shipboard industrial fire safety based on the Complex Systems Governance (CSG) reference model developed by Keating and Bradley (2015). The value of a clearly defined governance model and SMS framework in conjunction with industry standardization and information sharing is the emergence of trends. This supports feedback loops between requirements and outcomes, allowing more effective management of fire safety across the broad stakeholder groups involved. Prepared for the Naval Postgraduate School, Monterey, CA 93943. Naval Postgraduate School Approved for public release; distribution is unlimited. Approved for public release; distribution is unlimited.

Asphalt pavement is widely used in the US. The Volatile Organic Compounds (VOCs) volatized and emitted during its life cycle phase, including asphalt production, transportation, pavement paving, operation, as well as the demolition and reclaim, have a great effect on human health and the environment. Many studies have focused on the calculation, collecting, testing, and characteristics of VOC emissions from the asphalt pavement, however, several limitations still exist. For example, the database and the calculation methods are insufficient, as the calculation boundary is limited in the production phase and cannot extend to the lifecycle. To help the government to constrain the VOCs emissions within the legal system and draw more attention to the asphalt pavement reduction, this paper first defines the asphalt type, emission type and VOC category, along with the divided phases of the emissions. Then, a general database of VOCs emissions of asphalt pavement is established based on the data from the Environmental Protection Agency (EPA), European Environmental Agency (EEA) and other resources. Thirdly, a calculation method based on the coefficients is proposed to measure and compare the emissions from different types of asphalt under different conditions. Finally, this paper used an example project to test the reliability and feasibility of the method, as well as come up with suggestions for the related stakeholders, such as government, contractors and owners.

Naval Postgraduate School Acquisition Research Program

A Symposium Presentation Naval Postgraduate School Naval Postgraduate School Acquisition Research Program