1. Molecular Typing ofStaphylococcus aureusand Methicillin-ResistantS. aureus(MRSA) Isolated from Animals and Retail Meat in North Dakota, United States
- Author
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Esra Büyükcangaz, Valeria Velasco, Catherine M. Logue, Julie S. Sherwood, Ryan J. Koslofsky, Ryan M Stepan, Uludağ Üniversitesi/Veterinerlik Fakültesi/Mikrobiyoloji Anabilim Dalı., Büyükcangaz, Esra, and AAL-2323-2020
- Subjects
Veterinary medicine ,Adenosine ,Antibiotic resistance ,Swine ,Penicillin resistance ,Molecular typing ,Antimicrobial susceptibility ,Poultry ,5'-N-methylcarboxamideadenosine ,Suidae ,Anti-Infective Agents ,Kanamycin ,Bacterial transmission ,Genetic similarity ,Gentamicin ,Antiinfective agent ,Analogs and derivatives ,food and beverages ,Microbial sensitivity test ,Staphylococcal Infections ,Chicken ,Erythromycin ,Lincomycin ,Panton-Valentine leukocidin ,Electrophoresis, Gel, Pulsed-Field ,Pulsed field gel electrophoresis ,Staphylococcus aureus ,North Dakota ,Penicillin derivative ,Streptomycin ,Leukocidin ,Animals ,Meticillin ,Cefoxitin ,Food control ,Beef ,Field gel-electrophoresis ,Nose smear ,Human ,Methicillin-Resistant Staphylococcus aureus ,Exotoxin ,Exotoxins ,Microbial Sensitivity Tests ,Microbiology ,Article ,Bovinae ,Genetics ,Humans ,Poultry Diseases ,Korea ,Sheep ,Animal ,Dalfopristin plus quinupristin ,Bacterial toxin ,Tetracycline ,United States ,Genes ,Gene identification ,Raw meat ,Bacterial RNA ,Cattle ,Animal Science and Zoology ,Methicillin susceptible Staphylococcus aureus ,Nucleotide sequence ,Multilocus Sequence Typing ,Ovis aries ,Bacterium identification ,Broth dilution ,Multiplex polymerase chain reaction ,Food contamination ,Drug resistance ,Multidrug resistance ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Ciprofloxacin ,Leukocidins ,Drug Resistance, Multiple, Bacterial ,Prevalence ,DNA,bacterial ,Pork ,Priority journal ,Swine Diseases ,Broth microdilution ,Sus ,Classification ,Bacterial Typing Techniques ,Chemistry ,Veterinary ,Pigs ,Bacterium isolation ,DNA, Bacterial ,Bacterium isolate ,Meat ,Food industry ,RNA 16S ,Bacterial Toxins ,Sheep Diseases ,Biology ,Food science & technology ,medicine ,Animalia ,Food microbiology ,Bovine mastitis ,Drug effects ,Cow ,Bacterial DNA ,Bacterium culture ,Nonhuman ,Multiple drug resistance ,Chloramphenicol ,Isolation and purification ,Strain St398 ,Antibiotic sensitivity ,Food Microbiology ,Multilocus sequence typing ,Controlled study ,Chickens ,Panton Valentine leukocidin ,Methicillin resistant Staphylococcus aureus ,Food Science - Abstract
The objective of this study was to determine the prevalence and molecular typing of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in food-producing animals and retail meat in Fargo, North Dakota. A two-step enrichment followed by culture methods were used to isolate S. aureus from 167 nasal swabs from animals, 145 samples of retail raw meat, and 46 samples of deli meat. Positive isolates were subjected to multiplex polymerase chain reaction in order to identify the genes 16S rRNA, mecA, and Panton-Valentine Leukocidin. Pulsed-field gel electrophoresis and multilocus sequence typing were used for molecular typing of S. aureus strains. Antimicrobial susceptibility testing was carried out using the broth microdilution method. The overall prevalence of S. aureus was 37.2% (n = 133), with 34.7% (n = 58) of the animals positive for the organism, and the highest prevalence observed in pigs (50.0%) and sheep (40.6%) (p < 0.05); 47.6% (n = 69) of raw meat samples were positive, with the highest prevalence in chicken (67.6%) and pork (49.3%) (p < 0.05); and 13.0% (n = 6) of deli meat was positive. Five pork samples (7.0%) were positive for MRSA, of which three were ST398 and two were ST5. All exhibited penicillin resistance and four were multidrug resistant (MDR). The Panton-Valentine Leukocidin gene was not detected in any sample by multiplex polymerase chain reaction. The most common clones in sheep were ST398 and ST133, in pigs and pork both ST398 and ST9, and in chicken ST5. Most susceptible S. aureus strains were ST5 isolated from chicken. The MDR isolates were found in pigs, pork, and sheep. The presence of MRSA, MDR, and the subtype ST398 in the meat production chain and the genetic similarity between strains of porcine origin (meat and animals) suggest the possible contamination of meat during slaughtering and its potential transmission to humans. College of Agriculture, Food Systems and Natural Resources College, North Dakota State University College of Veterinary Medicine, Iowa State University
- Published
- 2013
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