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Additional file 1: Text S1. Methodology used for the different molecular characterization of Triatominae, blood source meal and Trypanosoma. Table S1. Description of primers used in molecular characterization of triatomines, blood source meals and Trypanosoma. Table S3. Distribution of the insects by capture points and developmental stages. Figure S1. a Environmental description of capture points in Trinidad, San Joaquin and San Borja. b Environmental description of capture points in Riberalta, Guayaramerín and Cobija. Table S4. General description of the environment around each capture point inside a circle of 500 m in radius. Table S5. Description of the haplotypes found for the Cytb, 16S, and 28S-D2 gene fragments. Figure S2. Maximum likelihood tree constructed with the 17 Cytb haplotypes jointly with < 100% coverage sequences deposited in GenBank. Figure S3. Maximum likelihood tree constructed with the eight 16S haplotypes jointly with sequences deposited in GenBank. Figure S4. Maximum likelihood tree constructed with the four 28S-D2 haplotypes jointly with sequences deposited in GenBank. Figure S5. Agarose gel electrophoresis (3% agarose) of PCR-amplified products using mini-exon multiplex PCR (MMPCR).