Search

This is a systematic review funded by Marie Curie with the following objectives: 1. To identify the patient-centred outcome measures implemented in palliative care clinical practice; 2. To identify the facilitators of implementing patient-centred outcome measures in palliative care clinical practice; 3. To identify the barriers to implementing patient-centred outcome measures in palliative care clinical practice; 4. To identify lessons learned on implementing patient-centred outcome measures in palliative care clinical practice; 5. To identify the implementation models used when implementing patient-centred outcome measures in palliative care clinical practice; 6. To identify what implementation outcomes were measured and how, when implementing patient-centred outcome measures in palliative care clinical practice; 7. To assess the financial costs of implementing patient-centred outcome measures in palliative care clinical practice; 8. To update the recommendations from our previous literature to inform the implementation process in palliative care clinical practice for stakeholders at different levels.

Cohesin's association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being 'clamped' by Scc2 and ATP-dependent engagement of cohesin's Smc1 and Smc3 head domains. Scc2's replacement by Pds5 abrogates cohesin's ATPase and has an important role in halting DNA loop extrusion. The ATPase domains of all SMC proteins are separated from their hinge dimerisation domains by 50-nm-long coiled coils, which have been observed to zip up along their entire length and fold around an elbow, thereby greatly shortening the distance between hinges and ATPase heads. Whether folding exists in vivo or has any physiological importance is not known. We present here a cryo-EM structure of the apo form of cohesin that reveals the structure of folded and zipped-up coils in unprecedented detail and shows that Scc2 can associate with Smc1's ATPase head even when it is fully disengaged from that of Smc3. Using cysteine-specific crosslinking, we show that cohesin's coiled coils are frequently folded in vivo, including when cohesin holds sister chromatids together. Moreover, we describe a mutation (SMC1D588Y) within Smc1's hinge that alters how Scc2 and Pds5 interact with Smc1's hinge and that enables Scc2 to support loading in the absence of its normal partner Scc4. The mutant phenotype of loading without Scc4 is only explicable if loading depends on an association between Scc2/4 and cohesin's hinge, which in turn requires coiled coil folding.

A composition comprising: a dye transfer inhibition agent, and, a ligand having the structure of formula (I), wherein: B1, B3 and B4 each represent a bridging group having zero, one two or three carbon containing nodes for substitution, and B2 represents a bridging group having at least one carbon containing node for substitution, each said node containing a C(R), C(R1)(R2) or C(R)2; each R substituent is the same is the same or different from the remaining R substituents and (1) is selected from the group consisting of alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, alkynyl, alkylaryl, halogen, alkoxy, phenoxy and combinations thereof, or (2) form a substituted or unsubstituted benzene ring of which two carbons on the ring form nodes in the B-unit. Preferably further comprising surfactant and builder, optional peroxygen source. Preferred dye transfer inhibition agents are dye binding polymers selected from polymers and co-polymers of vinylpyrrolidone, vinylpyridine N-oxide, and vinylimidazole, and mixtures thereof.