1. Oceanobacillus chungangensis sp. nov., isolated from a sand dune
- Author
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Beom Joon Kim, Hyeonji Kang, Veeraya Weerawongwiwat, Young-Wan Choi, Wonyong Kim, and Dong Chae Lee
- Subjects
DNA, Bacterial ,Molecular Sequence Data ,Biology ,Diaminopimelic Acid ,Microbiology ,Cell wall ,chemistry.chemical_compound ,Glycolipid ,RNA, Ribosomal, 16S ,Republic of Korea ,Bacillaceae ,Ecology, Evolution, Behavior and Systematics ,Phospholipids ,Phylogeny ,chemistry.chemical_classification ,Base Composition ,Strain (chemistry) ,Phylogenetic tree ,Fatty Acids ,Fatty acid ,Nucleic Acid Hybridization ,Vitamin K 2 ,General Medicine ,Sequence Analysis, DNA ,16S ribosomal RNA ,Silicon Dioxide ,Oceanobacillus ,Bacterial Typing Techniques ,chemistry ,Peptidoglycan - Abstract
A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1051T, was isolated from a sand dune and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1051T grew optimally at pH 5.0 and 30 °C. NaCl was not required for growth but up to 10.0 % (w/v) NaCl was tolerated. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1051T formed a distinct lineage within the genus Oceanobacillus and was most closely related to Oceanobacillus profundus CL-MP28T, Oceanobacillus caeni S-11T, and Oceanobacillus picturae LMG 19492T (96.8 %, 95.6 % and 95.3 % similarity, respectively). DNA–DNA reassociation analysis showed that strain CAU 1051T displayed 28.2±0.7 % relatedness to O. profundus KCTC 13625T. Strain CAU 1051T contained MK-7 as the only isoprenoid quinone and anteiso-C15 : 0 as the major fatty acid. The cell wall peptidoglycan of strain CAU 1051T contained meso-diaminopimelic acid. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, six unidentified phospholipids, an unidentified glycolipid, and six unidentified polar lipids. The major whole-cell sugars were glucose and ribose. The DNA G+C content was 36.3 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1051T represents a novel species of the genus Oceanobacillus for which the name Oceanobacillus chungangensis sp. nov. is proposed. The type strain is CAU 1051T ( = KCTC 33035T = CCUG 63270T).
- Published
- 2013