1. Recombinant murine steel factor stimulates in vitro production of granulocyte-macrophage progenitor cells
- Author
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S. D. Lyman, Eisenman J, Teepe M, D Foxworthe, Douglas E. Williams, and Anderson D
- Subjects
medicine.medical_treatment ,Population ,Stem cell factor ,Biology ,Granulocyte-Macrophage Progenitor Cells ,Hematopoietic Cell Growth Factors ,Biochemistry ,Mice ,medicine ,Animals ,Progenitor cell ,education ,Molecular Biology ,education.field_of_study ,Stem Cell Factor ,Growth factor ,Macrophages ,Cell Biology ,Molecular biology ,Mice, Mutant Strains ,Recombinant Proteins ,Hematopoiesis ,Mice, Inbred C57BL ,Haematopoiesis ,medicine.anatomical_structure ,Immunology ,Bone marrow ,Stem cell ,Granulocytes - Abstract
The ability of murine Steel factor to promote the in vitro production of granulocyte-macrophage progenitor cells (CFU-GM) was examined in short-term liquid cultures. Bone marrow from C57BL/6J or Sl/Sld mice was placed in culture for seven days with either Steel factor alone or in the presence of IL-3. CFU-GM responsive to GM-CSF, IL-3, and CSF-1 were measured in the input population and again after 3 or 7 days in culture. Steel factor alone increased the number of all CUF-GM types as early as 3 days after culture initiation, with further increases at day 7. This effect was protentiated by the addition of IL-3. Production of CFU-GM by C57BL/6J or Sl/Sld marrow was comparable except for enhanced production of CSF-1 responsive progenitors by Sl/Sld marrow. A recombinant Sld protein was also shown to be equivalent to the wild-type protein in its capacity to promote CFU-GM production from normal bone marrow. © 1992 Wiley-Liss, Inc.
- Published
- 1992