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1. Diversité et composition phytochimique des plantes utilisées comme bâtonnets frotte-dents (cure-dents) par les Peuls de la commune de Tessékéré (Ferlo Nord, Sénégal)

Author

Emeline Houël, Bétémondji Désiré Diatta, Gilles Boëtsch, Mathieu Gueye, Ousmane Niass, Massamba Diouf, UMI 3189 Environnement, santé, sociétés, université Cheikh Anta Diop/CNRS/ Université de Bamako/CNRST (UMI 3189), Centre National de la Recherche Scientifique (CNRS), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), and ANR-11-LABX-0010,DRIIHM / IRDHEI,Dispositif de recherche interdisciplinaire sur les Interactions Hommes-Milieux(2011)

Subjects
2. Zero hunger, Pharmacology, 0303 health sciences, 03 medical and health sciences, OHMi Tessekere, 0302 clinical medicine, Complementary and alternative medicine, 030220 oncology & carcinogenesis, 15. Life on land, [SDE.ES]Environmental Sciences/Environmental and Society, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology
Abstract

Le bâtonnet frotte-dents est un outil d’hygiène buccodentaire en forme de fragment de matière végétale : tige, brindille, petite branche ou racine. Le but de cette étude était de déterminer la composition phytochimique et la teneur en grandes classes chimiques des tiges les plus utilisées comme bâtonnets frotte-dents chez les Peuls de Widou Thiengoli au Nord Ferlo (Sénégal). Des entretiens ouverts semi-structurés ont permis de recueillir les plantes les plus utilisées. L’indice de fidélité renseigne sur la convergence des usages quant à l’emploi des plantes comme bâtonnets frotte-dents comparé aux autres pratiques cosmétiques répertoriées. L’étude de la composition phytochimique des tiges a ciblé huit classes chimiques : stéroïdes, terpénoïdes, tanins, saponines, anthocyanines, leucoanthocyanidines, coumarines, émodines. Les tanins et les saponines sont très fréquents ; les terpénoïdes et les leucoanthocyanines presque inexistants. Un dosage des flavonoïdes, alcaloïdes et polyphénols, réalisé chez des extraits aqueux, a ensuite été effectué sur 12 plantes (huit familles). Les grandes teneurs en polyphénols et alcaloïdes sont mesurées chez Anogeissus leiocarpus, et la meilleure en flavonoïdes chez Commiphora africana.

Published
2021
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3. Biosynthetic investigation of γ-lactones in Sextonia rubra wood using in situ TOF-SIMS MS/MS imaging to localize and characterize biosynthetic intermediates

Author

Tingting Fu, Christophe Duplais, Nadine Amusant, Serge Della-Negra, Gregory L. Fisher, Grégory Genta-Jouve, Alain Brunelle, Emeline Houël, David Touboul, Duplais, Christophe, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Equipe Pharmacognosie (UMR 8638), Chimie Organique, Médicinale et Extractive et Toxicologie Expérimentale (COMETE - UMR 8638), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), UMR8608, ANR-15-CE29-0007,DEFIMAGE,Un grand défi pour l'imagerie par spectrométrie de masse(2015), ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), Université Paris-Sud - Paris 11 (UP11), Institut de Physique Nucléaire d'Orsay (IPNO), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université Paris Descartes - Paris 5 (UPD5), Physical electronics (PHI), Agence Nationale de la Recherche (France) [ANR-2015-CE29-0007-01 DEFIMAGE], 'Investissement d'Avenir' grant [ANR-10-LABX-25-01], China Scholarship Council (CSC) [201406310013], and Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)

Subjects
K50 - Technologie des produits forestiers, 0301 basic medicine, In situ, Metabolite, lcsh:Medicine, butanolides, Tandem mass spectrometry, ion mass-spectrometry, chemistry.chemical_compound, Acetals, 0302 clinical medicine, Tandem Mass Spectrometry, lcsh:Science, constituents, metabolites, ComputingMilieux_MISCELLANEOUS, Cellular localization, [PHYS]Physics [physics], alkene-alkyne pair, stem wood, rubrenolide, heartwood, rubrynolide, resolution, Multidisciplinary, Chemistry, Composition chimique, Wood, Biochemistry, Alkynes, [CHIM.CHEM]Chemical Sciences/Cheminformatics, Resolution (mass spectrometry), F60 - Physiologie et biochimie végétale, Alkenes, Sextonia rubra, Article, Bois, Lauraceae, 03 medical and health sciences, Polyketide, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, lcsh:R, Metabolic pathway, 030104 developmental biology, lcsh:Q, 030217 neurology & neurosurgery
Abstract

Molecular analysis by parallel tandem mass spectrometry (MS/MS) imaging contributes to the in situ characterization of biosynthetic intermediates which is crucial for deciphering the metabolic pathways in living organisms. We report the first use of TOF-SIMS MS/MS imaging for the cellular localization and characterization of biosynthetic intermediates of bioactive γ-lactones rubrynolide and rubrenolide in the Amazonian tree Sextonia rubra (Lauraceae). Five γ-lactones, including previously reported rubrynolide and rubrenolide, were isolated using a conventional approach and their structural characterization and localization at a lateral resolution of ~400 nm was later achieved using TOF-SIMS MS/MS imaging analysis. 2D/3D MS imaging at subcellular level reveals that putative biosynthetic γ-lactones intermediates are localized in the same cell types (ray parenchyma cells and oil cells) as rubrynolide and rubrenolide. Consequently, a revised metabolic pathway of rubrynolide was proposed, which involves the reaction between 2-hydroxysuccinic acid and 3-oxotetradecanoic acid, contrary to previous studies suggesting a single polyketide precursor. Our results provide insights into plant metabolite production in wood tissues and, overall, demonstrate that combining high spatial resolution TOF-SIMS imaging and MS/MS structural characterization offers new opportunities for studying molecular and cellular biochemistry in plants.

Published
2019
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4. Ecology of termites from the genus Nasutitermes (Termitidae: Nasutitermitinae) and potential for science-based development of sustainable pest management programs

Author

Alice M. S Rodrigues, Nadine Amusant, Isabelle Boulogne, Emeline Houël, Michaël Falkowski, Reginaldo Constantino, Université des Antilles (Pôle Guadeloupe), Université des Antilles (UA), Institut d'écologie et des sciences de l'environnement de Paris (iEES), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA), Universidade de Brasilia [Brasília] (UnB), Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP), Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Centre National de la Recherche Scientifique (CNRS)-EDF (EDF)-PIERRE FABRE-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Europe (ERDF OP), French Guiana Regional Council, Air Liquide Foundation, 'Investissement d'Avenir' grant [ANR-10-LABX-25-01], ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-PIERRE FABRE-EDF (EDF)-Centre National de la Recherche Scientifique (CNRS), Institut d'écologie et des sciences de l'environnement de Paris (IEES), Université des Antilles (UA)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), and ANR-10-LABX-25-01/10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010)

Subjects
K50 - Technologie des produits forestiers, 0106 biological sciences, Integrated pest management, Nasutitermes, Phylogénie, Identification, Entomology, Taxonomic history, Nasutitermes corniger, Plant Science, 01 natural sciences, Termitidae, Insecticide, Ecology, Composition chimique, Extrait d'origine végétale, Taxonomy (biology), L20 - Écologie animale, Organisme nuisible, Insecticide d'origine végétale, Distribution géographique, Biogeography, Biogéographie, Biology, 010603 evolutionary biology, Bois, J12 - Manutention, transport, stockage et conservation des produits forestiers, Antimicrobial and insecticidal botanical extracts, Ecology, Evolution, Behavior and Systematics, Extrait de bois, Taxonomie, 15. Life on land, biology.organism_classification, Lutte biologique, 010602 entomology, Sustainable management, Insect Science, Préservation du bois, Écologie animale, Conformation animale, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Agronomy and Crop Science
Abstract

International audience; The genus Nasutitermes is among the most abundant wood-feeding Termitidae and an extremely diverse and heterogeneous group in terms of its biogeography and morphology. Despite the major role of several Nasutitermes species as structural pests, the phylogenetic status of this genus is still unclear, along with a confused taxonomy and species identification remaining difficult. The first aim of this review was thus to gather and discuss studies concerning the taxonomic status of the genus Nasutitermes in order to clarify this crucial point. Then, our goal was to gain new insights into the management of N. corniger, considered to be the most economically detrimental pest of this genus in South America and a Nasutitermes model species, while filtering available information concerning its biology through the prism of termite control, as well as critically examine the existing methods. We indeed strongly believe that increasing our knowledge of this species’ biological strategies is the key to progress in the challenging question of their sustainable management.

Published
2016
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5. Treating leishmaniasis in Amazonia : a review of ethnomedicinal concepts and pharmaco-chemical analysis of traditional treatments to inspire modern phytotherapies

Author

Didier Stien, Guillaume Odonne, Geneviève Bourdy, Emeline Houël, Laboratoire Ecologie, Evolution, Interactions des Systèmes amazoniens (LEEISA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS), Ecologie des forêts de Guyane (UMR ECOFOG), Université des Antilles (UA)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD), Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Centre National de la Recherche Scientifique (CNRS)-EDF (EDF)-PIERRE FABRE-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Investissement d'avenir : CEBA ANR -10-LABX-25-01, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-PIERRE FABRE-EDF (EDF)-Centre National de la Recherche Scientifique (CNRS), IFREMER, LEEISA, CNRS, Université de Guyane (UG), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Université Pierre et Marie Curie (Paris 6), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)

Subjects
Health Knowledge, Attitudes, Practice, [SDV]Life Sciences [q-bio], Antiprotozoal Agents, Distribution (economics), médecine traditionnelle, traditional medicine, 01 natural sciences, ethnomedecine, Ethnomedecine, Interculturality, amazonie, Medicinal plants, Amazonia, Drug Discovery, Medicine, Humans, drug plant, Environmental planning, Leishmaniasis, amazonia, Pharmacology, Plants, Medicinal, Traditional medicine, General distribution, 010405 organic chemistry, business.industry, Amazon rainforest, Plant Extracts, Cultural group selection, 15. Life on land, South America, interculturality, medicine.disease, distribution indexese, 0104 chemical sciences, Cultural heritage, 010404 medicinal & biomolecular chemistry, Taxon, Treatment Outcome, plante médicinale, Distribution indexes, Neglected tropical diseases, Medicine, Traditional, business, Phytotherapy, medicinal plants
Abstract

Ethnopharmacological relevance Cutaneous and mucocutaneous leishmaniasis are neglected tropical diseases that occur in all intertropical regions of the world. Amazonian populations have developed an abundant knowledge of the disease and its remedies. Therefore, we undertook to review traditional antileishmanial plants in Amazonia and have developed new tools to analyze this somewhat dispersed information. Material and methods A literature review of traditional remedies for cutaneous/mucocutaneous leishmaniasis in the Amazon was conducted and the data obtained was used to calculate distribution indexes designed to highlight the most relevant uses in Amazonia. The cultural distribution index represents the distribution rate of a given taxon among different cultural groups and was calculated as the ratio of the number of groups using the taxon to the total number of groups cited. The geographical distribution index allowed us to quantify spatial distribution of a taxon’s uses in Amazonia and was calculated geometrically by measuring the average distance between the points where uses have been reported and the barycenter of those points. The general distribution index was defined as an arithmetic combination of the previous two and provides information on both cultural and spatial criteria. Results 475 use reports, concerning 291 botanical species belonging to 83 families have been gathered depicted from 29 sources. Uses concern 34 cultural groups. While the use of some taxa appears to be Pan-Amazonian, some others are clearly restricted to small geographical regions. Particular attention has been paid to the recipes and beliefs surrounding treatments. Topical application of the remedies dominated the other means of administration and this deserves particular attention as the main treatments against Neotropical leishmaniasis are painful systemic injections. The data set was analyzed using the previously defined distribution indexes and the most relevant taxa were further discussed from a phytochemical and pharmacological point of view. Conclusions The Amazonian biodiversity and cultural heritage host a fantastic amount of data whose systematic investigation should allow a better large-scale understanding of the dynamics of traditional therapies and the consequent discovery of therapeutic solutions for neglected diseases. Distribution indices are indeed powerful tools for emphasizing the most relevant treatments against a given disease and should be very useful in the meta-analysis of other regional pharmacopeia. This focus on renowned remedies that have not yet benefitted from extended laboratory studies, could stimulate future research on new treatments of natural origin for leishmaniasis.

Published
2017
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6. Diversity of the Volatile Organic Compounds Emitted by 55 Species of Tropical Trees: a Survey in French Guiana

Author

Christopher Baraloto, Jean-Marie Bessière, Jérôme Chave, Emeline Houël, Didier Stien, Timothy Charles Eliot Paine, Elodie A. Courtois, Pierre-Alain Blandinières, Evolution et Diversité Biologique (EDB), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), UMR 5076, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 0106 biological sciences, Databases, Factual, Range (biology), Rainforest, 01 natural sciences, Biochemistry, Trees, 03 medical and health sciences, chemistry.chemical_compound, Species Specificity, Botany, Tropical climate, Cluster Analysis, Organic Chemicals, ComputingMilieux_MISCELLANEOUS, Ecology, Evolution, Behavior and Systematics, SESQUITERPENES, 030304 developmental biology, Tropical Climate, 0303 health sciences, alpha-Pinene, biology, Ecology, Data Collection, Laurales, VOCs, Plant community, General Medicine, Interspecific competition, 15. Life on land, biology.organism_classification, CHEMICAL DIVERSITY, French Guiana, Plant Leaves, chemistry, Plant Bark, Volatilization, 010606 plant biology & botany, Woody plant
Abstract

Volatile organic compounds (VOCs) are produced by a broad range of organisms, from bacteria to mammals, and they represent a vast chemical diversity. In plants, one of the preeminent roles of VOCs is their repellent or cytotoxic activity, which helps the plant deter its predators. Most studies on VOCs emitted by vegetative parts have been conducted in model plant species, and little is known about patterns of VOC emissions in diverse plant communities. We conducted a survey of the VOCs released immediately after mechanical damage of the bark and the leaves of 195 individual trees belonging to 55 tropical tree species in a lowland rainforest of French Guiana. We discovered a remarkably high chemical diversity, with 264 distinct VOCs and a mean of 37 compounds per species. Two monoterpenes (alpha-pinene and limonene) and two sesquiterpenes (beta-caryophyllene and alpha-copaene), which are known to have cytotoxic and deterrent effects, were the most frequent compounds in the sampled species. As has been established for floral scents, the blend of VOCs is largely species-specific and could be used to discriminate among 43 of the 55 sampled species. The species with the most diverse blends were found in the Sapindales, Laurales, and Magnoliales, indicating that VOC diversity is not uniformly distributed among tropical species. Interspecific variation in chemical diversity was caused mostly by variation in sesquiterpenes. This study emphasizes three aspects of VOC emission by tropical tree species: the species-specificity of the mixtures, the importance of sesquiterpenes, and the wide-ranging complexity of the mixtures.

Published
2009
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7. From Tonic-cups to Bitter-cups: Kwasi bita beker from Suriname

Author

Lionel Chevolot, Jacques Beauchêne, Guillaume Odonne, Didier Stien, Emeline Houël, Geneviève Bourdy, and Eric Deharo

Subjects
Pharmacology, Long lasting, Folk medicine, 0303 health sciences, Cultural attitudes, biology, Traditional medicine, business.industry, Materials testing, Ancient history, biology.organism_classification, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, 030220 oncology & carcinogenesis, Quassia, Drug Discovery, Medicine, Quassia amara, Simaroubaceae, sense organs, business, 030304 developmental biology
Abstract

In the main markets of Paramaribo (Suriname), many stands offer what is locally called "Bitter-cups", or "Kwasi bita beker", small footed-cups, roughly carved from a whitish wood. The use of these cups is strictly medicinal and it seems to be restricted to Suriname, as they are not found in neighbouring countries (Guyana, French Guiana). The aim of this study was to identify the botanical origin of Bitter-cups still in use in the Saramaka traditional medicine (as information from field people was controversial), and document the ethnopharmacology of this original galenical artefact. Microscopic and high performance liquid chromatography (HPLC) analyses were carried out on Bitter-cup, and anatomical criteria (marginal parenchyma band, size of intervessel and vessel-ray pits, rays width and rays composition, vessels clustering, frequency and size of parenchyma pits) together with HPLC profiles of the macerates showed that the wood cup was similar to Quassia amara L. (Simaroubaceae) wood. Ethnopharmacological investigation indicates that the use of these cups is simply due to the pharmacological properties attributed to "bitters", and is strongly linked to tradition and cultural attitudes. This study also emphasizes the long lasting use of these cups, now restricted to Suriname only, with almost no variation over one century.

Published
2007
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9. TakamalaImë (Psidium acutangulum Mart ex. DC): Understanding the use of an antimalarial traditional remedy from French Guiana

Author

Eric Deharo, Valérie Jullian, Véronique Eparvier, Marie Fleury, Pascal Villa, Didier Stien, Emeline Houël, Guillaume Odonne, Flore Nardella, Catherine Vonthron-Sénécheau, Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Patrimoines Locaux et Gouvernance (PALOC), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Laboratoire d'Innovation Thérapeutique (LIT), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Plate-forme de chimie biologique intégrative de Strasbourg (PCBiS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Ecologie, Evolution, Interactions des Systèmes amazoniens (LEEISA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), PIERRE FABRE-EDF (EDF)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), CNRS, UMR Ecol Forets Guyane, Kourou, France, Partenaires INRAE, UMR Botanique et bioinformatique de l'architecture des plantes, Institut de Recherche pour le Développement (IRD), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut de Recherche pour le Développement (IRD), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC), PCBIS Plate-forme de chimie biologique intégrative de Strasbourg, UMS 3286, CNRS, Université de Strasbourg, Labex Medalis, 300 boulevard Sebastien Brant, F-67000 Strasbourg, France, CNRS Guyane (USR 3456), and Centre National de la Recherche Scientifique (CNRS)

Subjects
Pharmacology, Psidium, Traditional medicine, business.industry, [SDV]Life Sciences [q-bio], Organic Chemistry, Pharmaceutical Science, Analytical Chemistry, Complementary and alternative medicine, Drug Discovery, Molecular Medicine, Medicine, business, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014
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11. In vitro antidermatophytic activity of Otacanthus azureus (Linden) Ronse essential oil alone and in combination with azoles

Author

Eric Deharo, Emeline Houël, Jean-Marie Bessière, Alice M. S. Rodrigues, Didier Stien, Véronique Eparvier, Arnaud Jahn-Oyac, Centre National de la Recherche Scientifique (CNRS), Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Systèmes d'Information Chimique (LSIC - ENSCM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Université des Antilles (UA)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Laboratoire de Phytochimie, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Pharmacochimie des substances naturelles et pharmacophores redox, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)

Subjects
Antifungal Agents, Magnetic Resonance Spectroscopy, synergy, Applied Microbiology and Biotechnology, law.invention, Trichophyton, law, Chlorocebus aethiops, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, 0303 health sciences, biology, Traditional medicine, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Broth microdilution, Drug Synergism, Plantaginaceae, General Medicine, Antimicrobial, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Ketoconazole, Biotechnology, medicine.drug, dermatophytes, Itraconazole, azoles, Microbial Sensitivity Tests, Gas Chromatography-Mass Spectrometry, essential oil, Microbiology, 03 medical and health sciences, medicine, Oils, Volatile, Animals, Dermatomycoses, Humans, [CHIM]Chemical Sciences, Vero Cells, Essential oil, 030304 developmental biology, 030306 microbiology, Arthrodermataceae, antifungal activity, Fungi, Otacanthus azureus, Plant Components, Aerial, biology.organism_classification, chemistry, Azole, Fluconazole
Abstract

International audience; AIMS: We determined the chemical composition and investigated the antifungal activity of Otacanthus azureus (Linden) Ronse essential oil (EO) against a range of dermatophytes alone or in combination with azole antifungals. METHODS AND RESULTS: Aerial parts of the plant were steam-distilled and the obtained oil was analysed by gas chromatography/mass spectrometry and (1) H-NMR. It was shown to be largely composed of sesquiterpenes, with the main component being β-copaen-4-α-ol. Using broth microdilution techniques, this oil was found to have remarkable in vitro antifungal activities. Minimum inhibitory concentrations as low as 4 μg ml(-1) were recorded. The analysis of the combined effect of the O. azureus EO with azoles using chequerboard assays revealed a synergism between the EO and ketoconazole, fluconazole or itraconazole against Trichophyton mentagrophytes. Notably, the O. azureus essential oil showed low cytotoxicity to VERO cells. CONCLUSIONS: The O. azureus essential oil alone or in combination with azoles is a promising antifungal agent in the treatment for human dermatomycoses caused by filamentous fungi. SIGNIFICANCE AND IMPACT OF THE STUDY: There is much interest in the study of essential oils for the discovery of new antimicrobial drugs. This study has highlighted the antidermatophytic activity of the O. azureus EO.

Published
2014
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13. In vitro antifungal activity of Otacanthus azureus (Linden) Ronse essential oil alone and in combination with azoles

Author

Emeline Houël, Didier Stien, Véronique Eparvier, Eric Deharo, Jean-Marie Bessière, A Rodrigues, and Arnaud Jahn-Oyac

Subjects
Pharmacology, Antifungal, Traditional medicine, Chemistry, medicine.drug_class, Stereochemistry, Organic Chemistry, Pharmaceutical Science, In vitro, Analytical Chemistry, law.invention, Complementary and alternative medicine, law, Drug Discovery, medicine, Molecular Medicine, Otacanthus azureus, Essential oil
Published
2012
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15. Evaluation of natural products as potential agrochemical agents with insecticide, fungicide and herbicide activities

Author

O Sorgenfrei, Philippe Rasoanaivo, Didier Stien, Nikolas Fokialakis, Marc Litaudon, Vincent Dumontet, Emeline Houël, Françoise Guéritte, A Espinosa, Florent Olivon, Mahabir P. Gupta, Véronique Eparvier, AL Skaltsounis, Gerda Fouche, D Breuninger, Maria Halabalaki, Cyril Poullain, Dionisio Olmedo, and Matthias Hamburger

Subjects
Pharmacology, Agrochemical, business.industry, Organic Chemistry, Pharmaceutical Science, Natural (archaeology), Analytical Chemistry, Biotechnology, Fungicide, Complementary and alternative medicine, International congress, Drug Discovery, Molecular Medicine, Environmental science, business, Plant Sources
Abstract

The International Congress on Natural Products Research, New York City, 28 July - 1 August 2012

Published
2012
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16. Quassinoid constituents of Quassia amara L. leaf herbal tea. Impact on its antimalarial activity and cytotoxicity

Author

Séverine Chevalley, Valérie Jullian, Stéphane Bertani, Didier Stien, Geneviève Bourdy, Eric Deharo, Emeline Houël, Alexis Valentin, Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Pharmacochimie des substances naturelles et pharmacophores redox, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, UMR 152, Institut de Recherche pour le Développement (IRD), USM 0307, Laboratoire de Parasitologie Comparée et Modèles Expérimentaux, and Muséum national d'Histoire naturelle (MNHN)

Subjects
Cell Survival, Cytotoxicity, [SDV]Life Sciences [q-bio], Plasmodium falciparum, Microbial Sensitivity Tests, Biology, Pharmacognosy, Antimalarial activity, Beverages, 03 medical and health sciences, Herbal tea, chemistry.chemical_compound, Antimalarials, 0404 agricultural biotechnology, Cell Line, Tumor, Drug Discovery, Quassia amara, Simalikalactone D, Humans, Medicinal plants, Medicine, African Traditional, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Pharmacology, 0303 health sciences, Traditional medicine, Molecular Structure, Quassins, Leaf tea, Plant Extracts, food and beverages, Quassia amara L, 04 agricultural and veterinary sciences, biology.organism_classification, 040401 food science, 3. Good health, Plant Leaves, Quassia, chemistry, Simaroubaceae, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Quassinoid, Quassin
Abstract

Aim of the study Our objective was to assess whether it could be contemplated to recommend Quassia amara young leaf tea for treatment against malaria, and if yes, set up a standard protocol for preparing the herbal tea. Materials and methods The leaf tea was extracted with methylene chloride and the organic extract was fractionated with HPLC. Pure compounds were characterized and their in vitro cytotoxicity and antiplasmodial activity was determined. Results and discussion We discovered that antimalarial Quassia amara young leaf tea contains several quassinoids: simalikalactone D (SkD, 1 ), picrasin B ( 2 ), picrasin H ( 3 ), neoquassin ( 4 ), quassin ( 5 ), picrasin I ( 6 ) and picrasin J ( 7 ). These last two compounds are new. In addition, our experiments demonstrate that both biological activity and cytotoxicity of the remedy may be attributed solely to the presence of SkD. Conclusion In conclusion, this preparation should not be recommended for treatment of malaria until a clinical study in humans is performed with SkD.

Published
2009
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17. Quassinoid constituents of Quassia amara L. (Simaroubaceae) leaf herbal tea. Impact on its antimalarial activity and cytotoxicity

Author

Eric Deharo, Didier Stien, Geneviève Bourdy, Emeline Houël, Séverine Chevalley, Stéphane Bertani, Alexis Valentin, and Valérie Jullian

Subjects
Pharmacology, biology, Traditional medicine, business.industry, Organic Chemistry, Pharmaceutical Science, Plasmodium falciparum, Context (language use), biology.organism_classification, Analytical Chemistry, Herbal tea, chemistry.chemical_compound, Complementary and alternative medicine, chemistry, Drug Discovery, Quassinoid, Molecular Medicine, Simalikalactone D, Quassia amara, Quassin, Medicine, Simaroubaceae, business
Abstract

French Guiana records high malaria incidence rates. The traditional antimalarial remedy most widespread and still very much in use there is a tea made out from Quassia amara mature leaves. The antimalarial activity of this preparation was assessed [1] and in order to optimize the in vitro activity, different types of preparation have been realized and tested. The most active in vitro preparation is an infusion of fresh young leaves. It demonstrated a very good activity, in vitro as well as in vivo [2]. A known quassinoid, simalikalactone D (SkD), was identified as the active compound, with an IC50 value of 10 nM against FcB1 Plasmodium falciparum chloroquine resistant strain in vitro [3]. Our next objective was to assess whether it could be contemplated to recommend this young leaves tea for treatment against malaria, since it seemed from literature precedent that SkD was also cytotoxic to a number of cellular lineages [4,5]. We then characterized and quantified the antiparasitic and cytotoxic activities of all the constituents. Several quassinoids were isolated and characterized in the tea: SkD, quassin, neoquassin, and picrasins B, H, I (new) and J (new), SkD being responsible of both antiplasmodial activity and cytotoxicity. In addition, in the context of an antimalarial treatment, it appeared that the dose necessary for obtaining a curative antimalarial effect is close to the toxic dose of an SkD analogue, bruceantin. Prior to emitting a definitive conclusion, a clinical study in humans similar to the one done with bruceantin [6] should be performed. References: 1. Bertani, S. et al. (2005)J. Ethnopharmacol. 98:45–54. 2. Bertani, S. et al. (2007)J. Ethnopharmacol.111:40–42. 3. Bertani, S. et al. (2006)J. Ethnopharmacol.108:155–157. 4. Ozeki, A. et al. (1998)J. Nat. Prod. 61:776–780. 5. Xu, Z. et al. (2000)J. Nat. Prod. 63:1712–1715. 6. Bedikian, A.Y. et al. (1979) Cancer Treat. Rep. 63:1843–1847.

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