73 results on '"F. Y. Yang"'
Search Results
2. [Effect analysis of treating intracranial wide-neck bifurcation aneurysms through Woven EndoBridge]
- Author
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F Y, Yang, J M, Liu, Q, Lyu, G, Wang, Y C, Tang, S Y, Du, X, Gao, and G B, Liang
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Male ,Treatment Outcome ,Endovascular Procedures ,Humans ,Female ,Intracranial Aneurysm ,Embolization, Therapeutic ,Retrospective Studies - Published
- 2022
3. [Analysis of the effect of stent-assisted embolization for low-grade subarachnoid hemorrhage caused by V4 segment dissecting aneurysm of vertebral artery]
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F Y, Yang, J M, Liu, X, Gao, H F, Zhang, Y S, Dong, J, Liu, M H, Zhou, and G B, Liang
- Published
- 2021
4. Magnetic Spectra of Soft Magnetic Composites Based on Fe-Si-Cr-B Amorphous and Carbonyl-Iron Powders
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Youwei Du, H. L. Su, F. Y. Yang, T.T. Xu, Y. Han, X. Y. Wu, M. M. Zhou, W.W. Guan, and Q. S. Meng
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010302 applied physics ,Materials science ,Composite number ,Demagnetizing field ,Condensed Matter Physics ,Inductor ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,Amorphous solid ,chemistry.chemical_compound ,Carbonyl iron ,chemistry ,Electrical resistivity and conductivity ,Permeability (electromagnetism) ,0103 physical sciences ,Composite material ,010306 general physics ,Phosphoric acid - Abstract
Fe–Si–Cr–B amorphous and carbonyl-iron powders insulated with phosphoric acid solution were used to prepare soft magnetic composites. The mass ratios of phosphoric acid insulation solution and the carbonyl-iron powder dependences of the magnetic spectrum, the density, and the resistivity were studied. Both the real part and the imaginary part of the core’s complex permeability increased after an initial decrease with the acid mass ratio increasing. This is similar to the variation tendency of the core’s density and opposite to the variation tendency of the core’s resistivity. The amount of the air gap, reflected by the density, was proposed to be the most important factor that has a great influence on the composite’s magnetic properties. The increase of the air gap reduced the effective demagnetizing field and the resistivity. This improved the permeability and the hysteresis loss and deteriorated the eddy-current loss. Carbonyl-iron powder addition was found to improve the moldability and the permeability of the composite based on the Fe-Si-Cr-B amorphous powder, although its loss was higher than that of the amorphous powder. All these findings can be referenced when designing an amorphous-based soft magnetic composite (SMC) core with balanced permeability, loss, and cost for high-frequency inductor within a MHz band.
- Published
- 2019
5. [The preliminary clinical study on radical prostatectomy without preoperative prostate biopsy]
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F Y, Yang, Y J, Li, S J, Han, D, Chen, L Y, Wu, Z J, Xiao, C L, Li, and N Z, Xing
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Male ,Prostatectomy ,Biopsy ,Positron Emission Tomography Computed Tomography ,Quality of Life ,Humans ,Middle Aged ,Prostate-Specific Antigen ,Aged - Published
- 2020
6. [Application of Endo-GIA stapler in laparoscopic radicalcystectomy]
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Q X, Zhao, F Y, Yang, D, Chen, L Y, Wu, M S, Wang, X S, Li, S, Wu, B K, Shi, and N Z, Xing
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Treatment Outcome ,Urinary Bladder Neoplasms ,Endometriosis ,Humans ,Female ,Laparoscopy ,Cystectomy ,Retrospective Studies - Published
- 2020
7. Dedifferentiation-reprogrammed mesenchymal stem cells for neonates with hypoxic-ischaemic brain injury
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F Y, Yang, X H, Zhang, L L, Tsang, H C, Chan, and X H, Jiang
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Rats, Sprague-Dawley ,Disease Models, Animal ,Animals, Newborn ,Hypoxia-Ischemia, Brain ,Animals ,Mesenchymal Stem Cells ,Rats - Published
- 2019
8. Clinical nursing intervention on severe pancreatitis after surgical treatment
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L, Wang, X Y, Sun, C L, Zhang, L J, Zhou, F Y, Yang, and M H, Shan
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Pancreatitis ,Acute Disease ,Humans - Published
- 2019
9. [Preliminary observation of galvanic vestibular stimulation-vestibular evoked myogenic potentials in healthy young people]
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Z C, Chen, X Y, Wei, Y Z, Zhang, Y, Cheng, F Y, Yang, C, Zhang, F Y, Chen, Y, Gao, J, Hu, M, Xu, and Q, Zhang
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Adult ,Male ,Young Adult ,Acoustic Stimulation ,Neck Muscles ,Oculomotor Muscles ,Humans ,Female ,Vestibule, Labyrinth ,Vestibular Evoked Myogenic Potentials ,Healthy Volunteers - Published
- 2019
10. [Evaluation of vestibular evoked myogenic potential, caloric test and cochlear electrogram in the diagnosis of Meniere's disease]
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M, Xu, Z C, Chen, X Y, Wei, Y Z, Zhang, F Y, Yang, C, Zhang, F Y, Chen, J, Hu, Y, Cheng, and Q, Zhang
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Caloric Tests ,Humans ,Vestibular Function Tests ,Sensitivity and Specificity ,Vestibular Evoked Myogenic Potentials ,Meniere Disease - Published
- 2019
11. [A case of acute toxic encophatopathy after uninfentio ally antifreeze ingestion]
- Author
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Y D, Tang, F Y, Yang, and N, Gong
- Published
- 2018
12. Cloning and expression analysis of three genes encoding ubiquitins in papaya (Carica papayaL.)
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K. Li, F. Y. Yang, J. J. Geng, Y. H. Shen, and X. J. Chen
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0106 biological sciences ,0301 basic medicine ,Cloning ,biology ,Sequence analysis ,food and beverages ,Ripening ,Horticulture ,Ribosomal RNA ,biology.organism_classification ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Ubiquitins ,030104 developmental biology ,Biochemistry ,chemistry ,Genetics ,Carica ,Gene ,010606 plant biology & botany ,Ethephon - Abstract
Papaya (Carica papaya L.) fruit have a short shelf-life due to rapid ripening induced by ethylene, which usually results in a high percentage of product loss. However, little is known about the genetic mechanism of ripening and the attributes of fruit quality. Ubiquitin (UBQ) proteins have received increasing attention because they play important roles in response to ripening and in regulating certain developmental processes in plants. In the present study, three genes encoding UBQ proteins, CpUBI1, CpUBI2, and CpUBI3, were isolated from papaya fruit. The lengths of the cDNAs of CpUBI1, CpUBI2, and CpUBI3 were 1,485 bp, 1,642 bp, and 529 bp, encoding 306, 308, and 156 predicted amino acids, respectively. Sequence analysis demonstrated that the CpUBI1 and CpUBI2 proteins contained four consecutive structural domains of the UBQ superfamily, while CpUBI3 contained a ribosomal domain structure of the S27a superfamily. RT-qPCR analysis demonstrated that ethephon treatment increased CpUBI gene expressio...
- Published
- 2016
13. Modeling and Prediction of Parking Location Selection of Passenger Drop-Off Area at Large Intermodal Transportation Terminals
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T Z Li and F Y Yang
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Transport engineering ,Computer science ,Drop (telecommunication) - Published
- 2018
14. A genome-wide DNA methylation analysis in peripheral blood from patients identifies risk loci associated with Graves' orbitopathy
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Xiu Tuo, L. Hua, X. Cao, T.-T. Shi, Jin-Kui Yang, Y. Li, F.-Y. Yang, and Z. Xin
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0301 basic medicine ,Oncology ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Logistic regression ,Graves' ophthalmopathy ,03 medical and health sciences ,Endocrinology ,Internal medicine ,medicine ,Humans ,Whole Genome Sequencing ,business.industry ,Genome, Human ,Incidence (epidemiology) ,Case-control study ,Methylation ,DNA Methylation ,Middle Aged ,medicine.disease ,Prognosis ,Graves Ophthalmopathy ,030104 developmental biology ,Genetic Loci ,Relative risk ,Case-Control Studies ,DNA methylation ,Human genome ,business ,Biomarkers ,Follow-Up Studies - Abstract
Graves’ orbitopathy (GO) is an inflammatory orbital disease of autoimmune origin with the potential to cause severe functional and psychosocial effects. The pathogenesis has not been fully elucidated. We investigated whether DNA methylation was associated with GO incidence in Chinese patients. Six GO patients and six age-matched controls were recruited, and genome-wide DNA methylation patterns were analyzed in their peripheral blood. t tests were performed to determine differential methylated sites in genomic regions and the univariable logistic regression analyses was performed to evaluate their risk with GO incidence. Cluster analysis and principal component analysis (PCA) were performed to determine the effects of the extracted differentially methylated sites. One hundred and forty-eight differentially methylated sites were identified, including CD14 (fold change = 4.31, p = 0.005), IL17RE (fold change = 2.128, p = 0.005), and DRD4 (fold change = 0.25, p = 0.004), and were supported by cluster and PCA analyses. Univariable logistic regression analyses showed that the methylation patterns at 12 loci were associated with GO incidence. The relative risk per 1% decrease in methylation at ZCCHC6 and GLI3 was 0.15 (95% CI 0.03–0.91; p = 0.039) and 0.65 (95% CI 0.42–0.98; p = 0.042), respectively. Pearson correlation analyses demonstrated that methylation levels at IL17RE were positively associated with Clinical Activity Score (CAS) (r = 0.967, p
- Published
- 2017
15. Convection Heat Transfer of CO2at Supercritical Pressures in Microtubes
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Taiyuan Liu, Ke Wang, Zunchao Liu, Yongqing Wang, and F.-Y. Yang
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Convection ,Natural convection ,Convective heat transfer ,Combined forced and natural convection ,Chemistry ,General Chemical Engineering ,Thermodynamics ,General Chemistry ,Heat transfer coefficient ,Rayleigh number ,Industrial and Manufacturing Engineering ,Supercritical fluid ,Forced convection - Published
- 2013
16. A Novel Approach to Simultaneous Measurements of Freshwater Ice Based on an Improved Monitor Apparatus
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X. R. Liu, H. B. Ma, W. N. Xu, D. Yin, M. L. Xu, and F. Y. Yang
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Freshwater ice ,Analytical chemistry ,Environmental science ,Electrical and Electronic Engineering ,Atomic and Molecular Physics, and Optics ,Remote sensing - Published
- 2013
17. HIGH GRADE GLIOMAS
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A. Leonard, J. Wolff, R. Sengupta, J. Marassa, D. Piwnica-Worms, J. Rubin, I. Pollack, R. Jakacki, L. Butterfield, H. Okada, J. Fangusaro, K. E. Warren, C. Mullins, P. Jurgen, S. Julia, C. C. Friedrich, S. Keir, J. Saling, M. Roskoski, H. Friedman, D. Bigner, C. Moertel, M. Olin, T. Dahlheimer, M. Gustafson, D. Sumstad, D. McKenna, W. Low, D. Nascene, A. Dietz, J. Ohlfest, D. Sturm, H. Witt, V. Hovestadt, D. A. K. Quan, D. T. W. Jones, C. Konermann, E. Pfaff, A. Korshunov, M. Rizhova, T. Milde, O. Witt, M. Zapatka, V. P. Collins, M. Kool, G. Reifenberger, P. Lichter, A. M. Lindroth, C. Plass, N. Jabado, S. M. Pfister, B. Pizer, A. Salehzadeh, A. Brodbelt, C. Mallucci, M. Brassesco, J. Pezuk, A. Morales, J. de Oliveira, G. Roberto, K. Umezawa, E. Valera, E. Rego, C. Scrideli, L. Tone, S. J. E. Veringa, D. G. Van Vuurden, P. Wesseling, W. P. Vandertop, D. P. Noske, T. Wurdinger, G. J. L. Kaspers, E. Hulleman, K. Wright, A. Broniscer, A. Bendel, D. Bowers, J. Crawford, P. Fisher, T. Hassall, G. Armstrong, J. Baker, I. Qaddoumi, G. Robinson, C. Wetmore, P. Klimo, F. Boop, A. Onar-Thomas, D. Ellison, A. Gajjar, O. Cruz, C. de Torres, M. Sunol, E. Rodriguez, L. Alonso, A. Parareda, T. Cardesa, H. Salvador, V. Celis, A. Guillen, G. Garcia, J. Muchart, C. Trampal, M. L. Martin, M. Rebollo, J. Mora, A. Piotrowski, A. Kowalska, P. Coyle, S. Smith, H. Rogers, D. Macarthur, R. Grundy, D. Puccetti, S. Salamat, T. Kennedy, N. Patel, K. Bradley, K. Casey, B. Iskandar, Y. Nakano, K. Okada, Y. Osugi, K. Yamasaki, H. Fujisaki, H. Fukushima, T. Inoue, Y. Matsusaka, H. Sakamoto, J. Hara, S. De Vleeschouwer, H. Ardon, F. Van Calenbergh, R. Sciot, G. Wilms, J. Van Loon, J. Goffin, S. Van Gool, D. Rusinak, P. Knight, K. Onel, D. Wargowski, A. Stettner, A. Al-Ghafari, W. Punjaruk, B. Coyle, I. Kerr, E. Xipell, M. Rodriguez, M. Gonzalez-Huarriz, M. T. Tunon, I. Zazpe, S. Tejada-Solis, R. Diez-Valle, J. Fueyo, C. Gomez-Manzano, M. M. Alonso, D. Pastakia, C. McCully, R. Murphy, J. Bacher, M. Thomas, E. Steffen-Smith, K. Saleem, S. Waldbridge, B. Widemann, K. Warren, E. Miele, F. Buttarelli, A. Arcella, F. Begalli, A. Po, C. Baldi, G. Carissimo, M. Antonelli, V. Donofrio, I. Morra, P. Nozza, A. Gulino, F. Giangaspero, E. Ferretti, I. Elens, F. Pauwels, S. Fritzell, S. Eberstal, E. Sanden, E. Visse, A. Darabi, P. Siesjo, P. McDonald, J. Wrogemann, S. Krawitz, M. Del Bigio, D. Eisenstat, R. Kwiecien, T. Pietsch, A. Faldum, R.-D. Kortmann, M. Warmuth-Metz, S. Rutkowski, I. Slavc, C. M. Kramm, U. Uparkar, R. Geyer, R. Ermoian, R. Ellenbogen, S. Leary, J. Triscott, K. Hu, A. Fotovati, S. Yip, R. Kast, B. Toyota, S. Dunn, M. Hegde, A. Corder, K. Chow, M. Mukherjee, A. Ashoori, V. Brawley, H. Heslop, S. Gottschalk, E. Yvon, N. Ahmed, T.-T. Wong, F.-Y. Yang, M. Lu, H.-F. Liang, H.-E. Wang, R.-S. Liu, M.-C. Teng, C.-C. Yen, S. Agnihotri, C. Ternamian, C. Jones, G. Zadeh, J. Rutka, C. Hawkins, I. Filipek, M. Drogosiewicz, M. Perek-Polnik, E. Swieszkowska, B. D. Baginska, E. Jurkiewicz, D. Perek, A. Kuehn, F. Falkenstein, A. Gnekow, C. Kramm, M. D. Brooks, E. Jackson, R. D. Mitra, J. B. Rubin, X.-Y. Liu, J. Schwartzentruber, A. M. Fontebasso, D.-A. K. Quang, S. Albrecht, Z. Dong, P. Siegel, A. Von Diemling, D. Faury, U. Tabori, J. Majewski, R. Lulla, M. Echevarria, T. Alden, A. DiPatri, T. Tomita, S. Goldman, T. Lin, T. E. Merchant, M. Kocak, A. P. Panandiker, G. T. Armstrong, G. H. Gielen, A. z. Muehlen, C. Hubert, Y. Ding, C. Toledo, P. Paddison, J. Olson, M. Nandhabalan, L. Bjerke, D. Bax, D. Carvalho, I. Bajrami, A. Ashworth, C. Lord, D. Hargrave, R. Reis, P. Workman, S. Little, S. Popov, A. Jury, A. Burford, L. Doey, S. Al-Sarraj, J. Jurgensmeier, L. Chen, I. Kozarewa, S. Baker, L. Perryman, G. Box, F. Raynaud, S. Eccles, M. Viana-Pereira, M. Pereira, T. Forshew, R. Tatevossian, D. Sheer, J. Pimental, M. Pires, C. Sarkar, P. Jha, I. R. P. Patrick, K. Somasundaram, P. Pathak, M. C. Sharma, V. Suri, A. Suri, N. Gerges, T. Haque, A. Nantel, C. Lee, J. Chen, C. Venugopal, A. Singhal, C. Dunham, J. Kerr, M. Verreault, H. Wakimoto, A. Jayanthan, A. Narendran, S. Singh, G. Giraud, S. Holm, B. Gustavsson, R. Kizyma, Z. Kizyma, L. Dvornyak, B. Kotsay, S. Epari, P. Sharma, M. Gurav, T. Gupta, P. Shetty, A. Moiyadi, S. Kane, and R. Jalali
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Abstracts ,03 medical and health sciences ,Cancer Research ,0302 clinical medicine ,Oncology ,business.industry ,030220 oncology & carcinogenesis ,Medicine ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Published
- 2012
18. Chain-scattering description approach to characterise stabilising controllers and optimal H2 solution
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Mi-Ching Tsai and F. Y. Yang
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Control and Optimization ,Coprime integers ,Process (engineering) ,Stability (learning theory) ,Optimal control ,Computer Science Applications ,Matrix decomposition ,Human-Computer Interaction ,Matrix (mathematics) ,Chain (algebraic topology) ,Control and Systems Engineering ,Control theory ,Control system ,Electrical and Electronic Engineering ,Mathematics - Abstract
This study presents an alternative method for solving a general class of control synthesis problems by employing chain-scattering matrix descriptions and coprime factorisations. Extended from Kimura's approach, the proposed method follows an abridged development without the need for any plant augmentation or the conjugation technique in its solution process. It is shown that general control synthesis problems, including those for stabilising controllers or further for H 2 controllers, can be represented via two associated chain-scattering matrix descriptions and solved by means of two coprime factorisations. In the two-port chain description framework, the coprime factorisation is utilised as the fundamental idea instead of J-spectrum factorisation from the perspective of facilitating the process of guaranteeing no unstable pole-zero cancellation. A significant contribution of this study is its transparency and intuitive nature, providing a systematic theory of control and allowing practicing control engineers to learn this approach more easily and apply it to advanced control systems. Related graphic network representations are also presented to help explain the whole concept which will benefit engineering readers.
- Published
- 2011
19. Anti-predator behaviour in the green-lipped mussel Perna viridis: byssus thread production depends on the mussel’s position in clump
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C. C. Liu, Siu Gin Cheung, Jill M.Y. Chiu, F. Y. Yang, and P.K.S. Shin
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animal structures ,Ecology ,biology ,fungi ,Mussel ,Aquatic Science ,biology.organism_classification ,Predation ,Shrimp ,Green lipped mussel ,Byssus ,Predator ,Ecology, Evolution, Behavior and Systematics ,Perna viridis - Abstract
Green-lipped mussels Perna viridis secrete a larger number of byssus threads to anchor themselves to the substrate more firmly when predation risks are high. These mussels also form clumps. As predators usually attack clumps from the periphery, the predation risks of solitary mussels are higher than those of mussels living along the edge of clumps, which are in turn higher than those of mussels living within the clumps. The byssus thread number, length and diameter were investigated in relation to the mussels’ position in the clump. We set up chemical stimulus treatments with damaged conspecifics and heterospecifics, suggesting predation risks, as well as with intact conspecifics and heterospecifics, shrimp cues and control (no cue). The mean number of byssus threads produced was significantly affected by both the mussels’ position in the clump, the treatment type, and the interactive effect between these 2 variables. In the treatment in which mussels were exposed to damaged conspecific cues, the mean number of byssus threads produced by the solitary mussels was greater than the number produced by edge mussels, which was, in turn, greater than the number produced by the mussels within the clump. Furthermore, the solitary and edge mussels exposed to damaged conspecifics secreted more byssus threads than their counterparts in the control, intact conspecifics, intact and damaged heterospecifics, and shrimp meat treatments. However, the mussels within the clumps exposed to damaged conspecifics did not secrete more byssus threads than their control counterparts. Results of the present study suggest that the anti-predator responses to risk may be related to the presence of damaged conspecifics and the mussels’ position in the clump, both indicative of local predation pressure.
- Published
- 2009
20. Effects of Dietary Energy Density on Growth, Carcass Quality and mRNA Expression of Fatty Acid Synthase and Hormone-sensitive Lipase in Finishing Pigs
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X. Q. Ou, Z. H. Liu, L. J. Kong, F. Y. Yang, X. S. Piao, Y. H. Gu, and C. H. Lai
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chemistry.chemical_classification ,medicine.medical_specialty ,biology ,Mrna expression ,Hormone-sensitive lipase ,Factorial experiment ,Crossbreed ,Amino acid ,Fatty acid synthase ,Animal science ,Endocrinology ,chemistry ,Internal medicine ,medicine ,biology.protein ,Animal Science and Zoology ,Intramuscular fat ,Lipase ,Food Science - Abstract
A single factorial experiment was conducted to test the effects of three dietary levels of energy on mRNA expression of fatty acid synthase (FAS-mRNA) and hormone-sensitive lipase (HSL-mRNA) and their association with intramuscular fat in finishing pigs. 72 crossbred (Large WhitexRongchang) barrows with an average initial body weight of 20.71 (s.e. 0.1) kg, were randomly allotted to three dietary treatments (11.75, 13.05 and 14.36 MJ DE/kg) and fed until slaughtered at 100 or 101 kg. The diets were iso-nitrogenous and iso-essential amino acids. The growth performances including the duration of finishing were changed linearly (p
- Published
- 2007
21. Non-extracorporeal circulation for coronary artery bypass graft surgery is more beneficial than extracorporeal circulation
- Author
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F-Y, Yang, Y-Z, Bao, F-S, Liu, Y-C, Zhu, J, Zheng, J-H, Zhang, X-F, Zheng, and G-C, Wei
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Adult ,Male ,Extracorporeal Circulation ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Interleukin-8 ,Humans ,Female ,Coronary Artery Bypass ,Inflammation Mediators ,Middle Aged ,Biomarkers ,Aged - Abstract
The objective of this study was to compare coronary artery bypass graft (CABG) surgery with non-extracorporeal vs. extracorporeal circulation. The study outcomes included operative time, number of graft vessels, pulmonary infection rates, and systemic inflammatory markers.96 patients received selective CABG, either with non-extracorporeal (study group; n = 48) or extracorporeal circulation (control group; n = 48). Operative time, pulmonary infection rates, and blood levels of inflammatory markers TNF-α, IL-6, and IL-8 before and 4, 24, and 48 hours after the surgery were quantified. Graft vessels were quantified using computed tomography.Operative time was significantly shorter in study group (4.58 ± 0.91 vs. 5.36 ± 1.12 hours in control group; p0.05). The number of graft vessels and pulmonary infection rates were comparable between both techniques. However, systemic inflammatory markers were significantly (p0.05) lower in study group at 4 and, partly, 24 hours after the surgery.Extracorporeal circulation prolongs operation and can aggravate systemic inflammatory response. Therefore, CABG with non-extracorporeal circulation offers more beneficial outcomes.
- Published
- 2015
22. A machine-order search space for job-shop scheduling problems
- Author
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F. Y. Yang and S. H. Choi
- Subjects
Rate-monotonic scheduling ,Earliest deadline first scheduling ,Mathematical optimization ,Theoretical computer science ,Job shop scheduling ,Computer science ,Strategy and Management ,Dynamic priority scheduling ,Flow shop scheduling ,Management Science and Operations Research ,Fair-share scheduling ,Computer Science Applications ,Management of Technology and Innovation ,Two-level scheduling ,Beam search ,Business and International Management - Abstract
This paper proposes a methodology for real-time job-shop scheduling problems. It introduces a new classification of the scheduling methods for JSSPs with emphasis on the search methods and the significance of the search space. Subsequently, a machine-order search space is proposed as a new framework in which different single-machine scheduling algorithms and search methods can be incorporated to solve JSSPs. An optimization model relating makespan minimization and the proposed machine-order search space is also described. The proposed methodology finds an optimal solution by searching a proper machine order in the machine-order search space and scheduling the machines one by one in this order. Such an approach significantly reduces the size of the search space, and hence the computing efforts. As a result, scheduling of large JSSPs in real-time becomes practicable.
- Published
- 2003
23. First Report of Streptomyces diastatochromogenes Causing Potato Common Scab in China
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D. J. Yang, Daqun Liu, Weiquan Zhao, F. Y. Yang, and Xiu-Mei Yu
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0106 biological sciences ,0301 basic medicine ,03 medical and health sciences ,Common scab ,Botany ,Streptomyces diastatochromogenes ,Plant Science ,030108 mycology & parasitology ,Biology ,01 natural sciences ,Agronomy and Crop Science ,010606 plant biology & botany - Published
- 2017
24. Changes of Transmembrane Ca2+ Gradient in the Formation of Macrophage-Derived Foam Cells
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Xiaoyi Yang, Youguo Huang, F. Y. Yang, and Yuzhong Zhang
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Male ,Biophysics ,Biochemistry ,Mice ,Cytosol ,Confocal laser scanning microscopy ,Animals ,Humans ,Macrophage ,Scavenger receptor ,Molecular Biology ,Cell Nucleus ,Ions ,Mice, Inbred BALB C ,Microscopy, Confocal ,Chemistry ,Macrophages ,Cell Membrane ,Cell Biology ,Flow Cytometry ,Transmembrane protein ,Up-Regulation ,Cell biology ,Lipoproteins, LDL ,Mice, Inbred C57BL ,Cholesterol ,Membrane ,Calcium ,Foam Cells ,Lipoprotein - Abstract
Macrophages from C57BL/6J mice, an animal susceptible to atherosclerosis, were chosen as target cells to study changes in the transmembrane Ca2+ gradient during the formation of macrophage-derived foam cells. The transmembrane Ca2+ gradients of single living cells were examined-using Fura-2/AM combined with Fluo-3/AM by laser scanning confocal microscopy. Exposure to Oxidized Low Density Lipoprotein, decreases the Ca2+ gradient across macrophage plasma membrane, but increases that across the nuclear membrane. The altered transmembrane Ca2+ gradients could induce the up-regulation of scavenger receptor in macrophages, resulting in the formation of foam cells.
- Published
- 2000
25. A method for uni-directional reconstitution of human erythrocyte glucose transporter
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L. Xiao, F. Y. Yang, and W. S. Xie
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Erythrocytes ,Monosaccharide Transport Proteins ,Proteolipids ,Detergents ,Clinical Biochemistry ,Phospholipid ,Biochemistry ,law.invention ,chemistry.chemical_compound ,Glucosides ,law ,Mole ,Genetics ,Freeze Fracturing ,Humans ,Molecular Biology ,Phospholipids ,Glucose Transporter Type 1 ,Liposome ,Chromatography ,biology ,Glucose transporter ,Transporter ,Cell Biology ,Kinetics ,Microscopy, Electron ,Glucose ,chemistry ,Permeability (electromagnetism) ,Liposomes ,biology.protein ,GLUT1 ,Electron microscope - Abstract
The glucose transporter of human erythrocytes (Glut1) was reconstituted into soybean phospholipid liposomes by a method of direct incorporation using the nonionic detergent n-octyl beta-D-glucopyranoside. The reconstituted proteoliposomes were proved to be intact and low ionic permeability. Freeze-fracture electron microscopy study showed that the diameter of the proteoliposomes was about 150 +/- 50 nm and the protein was randomly distributed. The kinetic parameters of the reconstituted transporter were: Km =16.23 mmol/L, Vmax = 34.48 nmol/sec x mg protein. Furthermore, about 90% of the glucose transporter in the reconstituted proteoliposomes were orientated inside-out. Until now it is a more efficient method for uni-directional reconstitution of Glut1 with good reproducibility and higher transport activity.
- Published
- 1998
26. Sensitivity of Ca2+ Transport of Mitochondria to Reactive Oxygen Species
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F. Y. Yang and Zhi-wei Yang
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Male ,Radical ,Biophysics ,Respiratory chain ,Fluorescence Polarization ,Oxidative phosphorylation ,Mitochondrion ,Biochemistry ,Mitochondria, Heart ,Oxidative Phosphorylation ,Membrane Potentials ,Cyclic N-Oxides ,chemistry.chemical_compound ,Superoxides ,Animals ,Ketoglutarate Dehydrogenase Complex ,Rats, Wistar ,Inner mitochondrial membrane ,Molecular Biology ,Free-radical theory of aging ,chemistry.chemical_classification ,Reactive oxygen species ,Ion Transport ,Hydroxyl Radical ,Superoxide ,Electron Spin Resonance Spectroscopy ,Cell Biology ,Rats ,chemistry ,Calcium ,Reactive Oxygen Species ,Diphenylhexatriene - Abstract
The relationship between Ca2+ transport and energy transduction of myocardial mitochondria in the presence of reactive oxygen species was investigated. Following treatment with oxygen free radicals [superoxide(O2•) or hydroxyl radical (•)OH], lipid free radicals in myocardial mitochondrial membrane could be detected by using the method of EPR spin trap. Simultaneously there were obvious alterations in the free Ca2+ ([Ca2+]m) in the mitochondrial matrix; the physical state of membrane lipid; the efficiency of oxidative phosphorylation (ADP/O); the value of the respiratory control ratio (RCR); and the membrane potential of the inner membrane of myocardial mitochondria. If the concentrations of reactive oxygen species were reduced by about 30%, the alterations in the physical state of the membrane lipid and energy transduction of myocardial mitochondria were not observed, but the changes in Ca2+ homeostasis remained. We conclude that Ca2+ transport by myocardial mitochondria is more sensitive to agents such as (O2•) or •OH, etc. than are oxidation phosphorylation and the respiratory chain.
- Published
- 1997
27. Lipids may not be involved in the recognition of apocytochrome c during its transportation
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Q. F. Ma and F. Y. Yang
- Subjects
Swine ,Membrane lipids ,Clinical Biochemistry ,Cytochrome c Group ,Mitochondria, Liver ,Mitochondrion ,Biology ,Apocytochrome C ,Biochemistry ,Mitochondria, Heart ,Outer mitochondrial membrane ,Genetics ,Animals ,Membrane vesicle ,Molecular Biology ,Vesicle ,Cell Membrane ,Cytochromes c ,Biological Transport ,Cell Biology ,Lipid Metabolism ,Rats ,Translocase of the inner membrane ,Microsomes, Liver ,Microsome ,lipids (amino acids, peptides, and proteins) ,Apoproteins - Abstract
It was shown that apocytochrome c was less insertion into monolayers from outer mitochondrial membrane lipids than into those from microsomal membrane lipids; the alpha-helix content of apocytochrome c induced by small unilamellar vesicles prepared from outer mitochondrial membrane lipids was less than by those from microsomal membrane lipids; the import efficiency of apocytochrome c into large unilamellar vesicles from outer mitochondrial membrane lipids was also lower than into those from microsome membrane lipids. No specific affinity between apocytochrome c and outer mitochondrial membrane lipids could be found. Import of apocytochrome c across the intact mitochondria, sealed outer mitochondrial membrane and microsome membrane vesicles was compared. Results showed that apocytochrome c was accumulated only in mitochondria, but not the other two kinds of vesicles.
- Published
- 1997
28. Probing the spin pumping mechanism: exchange coupling with exponential decay in Y3Fe5O12/barrier/Pt heterostructures
- Author
-
C H, Du, H L, Wang, Y, Pu, T L, Meyer, P M, Woodward, F Y, Yang, and P C, Hammel
- Abstract
It is widely believed that the mechanism for spin pumping in ferromagnet-nonmagnet bilayers is the exchange interaction between the ferromagnet and nonmagnetic material. We observe 1000-fold exponential decay of spin pumping from thin Y3Fe5O12 films to Pt across insulating barriers, from which exponential decay lengths of 0.16, 0.19, and 0.23 nm are extracted for oxide barriers having band gaps of 4.91, 3.40, and 2.36 eV, respectively. This archetypal signature of quantum tunneling through a barrier underscores the importance of exchange coupling for spin pumping and reveals its dependence on the characteristics of the barrier material.
- Published
- 2013
29. Se-mediated domain-domain communication in Band 3 of human erythrocytes
- Author
-
F. Y. Yang, C. Fen, and Y. P. Tu
- Subjects
Cytoplasm ,Conformational change ,Protein Conformation ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Cell Communication ,Biochemistry ,Fluorescence ,src Homology Domains ,Inorganic Chemistry ,Sodium Selenite ,Protein structure ,Anion Exchange Protein 1, Erythrocyte ,Humans ,Trypsin ,Sulfhydryl Compounds ,Band 3 ,Ion Transport ,Quenching (fluorescence) ,biology ,Chemistry ,Vesicle ,Erythrocyte Membrane ,Biochemistry (medical) ,Active site ,General Medicine ,Hydrogen-Ion Concentration ,Crystallography ,Membrane ,biology.protein - Abstract
Na2SeO3 could affect the anion flux of Band 3 of inside-out erythrocyte membrane vesicles (IOVs). Such effect was believed to be based on the interaction of SH groups of Band 3 with Na2SeO3. This effect could be eliminated when the cytoplasmic domain of Band 3 was proteolytically removed by trypsin. This suggested that SH groups in the cytoplasmic domain were involved in such interaction. Measurement of the pH dependence of intrinsic fluorescence intensity provided evidence that conformational changes of Band 3 occurred as a consequence of interaction with selenite. KI quenching of intrinsic fluorescence of Band 3 could also show that there was a conformational change in the cytoplasmic domain of Band 3 after reaction with Na2SeO3. Such conformational change in turn could be transmitted to the membrane domain of Band 3 monitored by quenching of intrinsic fluorescence of Band 3 using hypocrellin B (HB) (a photosensitive pigment obtained from a parasitic fungus growing in Yunnan, China). It is suggested that the cytoplasmic domain of Band 3 is not necessary for its anion flux, but is essential for the regulation (e.g., by Se) of its active site located at the membrane domain, and hence, it may provide evidence of communication between the cytoplasmic domain and the membrane domain of Band 3.
- Published
- 1996
30. Transmembrane Ca2+ gradient and function of membrane proteins
- Author
-
Y. G. Huang, F. Y. Yang, and Y. P. Tu
- Subjects
Ion Transport ,Chemistry ,Endoplasmic reticulum ,Biophysics ,Membrane Proteins ,Calcium-Transporting ATPases ,Cell Biology ,Biochemistry ,Transmembrane protein ,Cell biology ,Coupling (electronics) ,Sarcoplasmic Reticulum ,Membrane ,Membrane protein ,Physical state change ,Animals ,Humans ,Calcium ,Signal transduction ,Molecular Biology ,Function (biology) ,Signal Transduction - Abstract
This review will focus on the recent advance in the study of effect of transmembrane Ca2+ gradient on the function of membrane proteins. It consits of two parts: 1. Transmembrane Ca2+ gradient and sarcoplasmic reticulum Ca2+-ATPase; 2. Effect of transmembrane Ca2+ gradient on the components and coupling of cAMP signal transduction pathway. The results obtained indicate that a proper transmembrane Ca2+ gradient may play an important role in modulating the conformation and activity of SR Ca2+-ATPase and the function of membrane proteins involved in the cAMP signal transduction by mediating the physical state change of the membrane phospholipids.
- Published
- 1995
31. Fluorescence study on transmembrane Ca2+ gradient-mediated conformation changes of sarcoplasmic reticulum Ca2+-ATPase
- Author
-
Y. P. Tu and F. Y. Yang
- Subjects
Protein Conformation ,ATPase ,Biophysics ,Calcium-Transporting ATPases ,Photochemistry ,Biochemistry ,Fluorescence spectroscopy ,Membrane Potentials ,Pigment ,Animals ,Perylene ,Molecular Biology ,biology ,Chemistry ,Endoplasmic reticulum ,Vesicle ,Quinones ,Tryptophan ,Cell Biology ,Fluorescence ,Transmembrane protein ,Sarcoplasmic Reticulum ,Spectrometry, Fluorescence ,visual_art ,visual_art.visual_art_medium ,biology.protein ,Calcium ,Rabbits - Abstract
The conformational states of Ca2+-ATPase in sarcoplasmic reticulum (SR) vesicles with or without a thousand-fold transmembrane Ca2+ gradient have been studied by fluorescence spectroscopy and fluorescence quenching. In consequence of the establishment of the transmembrane Ca2+ gradient, the steady-state fluorescence results revealed a reproducible 8% decrease in the intrinsic fluorescence while time-resolved fluorescence measurements showed that 13 tryptophan residues in SR · Ca2+-ATPase could be divided into three groups. The fluorescence lifetime of one of these groups increased from 5.5 ns to 5.95 ns in the presence of a Ca2+ gradient. Using KI and hypocrellin B (a photosensitive pigment obtained from a parasitic fungus, growing in Yunnan, China), the fluorescence quenching further indicated that the dynamic change of this tryptophan group, located at the protein-lipid interface, is a characteristic of transmembrane Ca2+ gradient-mediated conformational changes in SR · Ca2+-ATPase.
- Published
- 1994
32. Divalent cation and lipid-protein interactions of biomembranes
- Author
-
Y. P. Tu, F. Y. Yang, and Y. G. Huang
- Subjects
chemistry.chemical_classification ,Cations, Divalent ,Cell Membrane ,Biophysics ,Phospholipid ,Membrane Proteins ,chemistry.chemical_element ,Adenylate kinase ,Biological membrane ,Intracellular Membranes ,Cell Biology ,Calcium ,Biochemistry ,Cyclase ,Transmembrane protein ,Protein–protein interaction ,Divalent ,Membrane Lipids ,chemistry.chemical_compound ,chemistry ,Animals ,Humans ,Molecular Biology - Abstract
Divalent cations play an important role in the functions of biomembranes. This review deals with three topics: (1) Mg2+-mediated change in physical state of phospholipid induces conformation and activity change of reconstituted mitochondrial H+-ATPase, (2) a proper transmembrane Ca2+ gradient is essential for the higher enzymatic activity of adenylate cyclase, and (3) role of transmembrane Ca2+ gradient in the modulation of reconstituted sarcoplasmic reticulm Ca2+-ATPase activity.
- Published
- 1993
33. Growth control of GaAs nanowires using pulsed laser deposition with arsenic over pressure
- Author
-
X W Zhao, A J Hauser, T R Lemberger, and F Y Yang
- Subjects
Condensed Matter - Materials Science ,congenital, hereditary, and neonatal diseases and abnormalities ,Materials science ,business.industry ,Mechanical Engineering ,Thermal decomposition ,Nanowire ,chemistry.chemical_element ,nutritional and metabolic diseases ,Materials Science (cond-mat.mtrl-sci) ,FOS: Physical sciences ,Bioengineering ,General Chemistry ,Pulsed laser deposition ,chemistry ,Mechanics of Materials ,Optoelectronics ,Molecule ,General Materials Science ,Crystallite ,Electrical and Electronic Engineering ,business ,Layer (electronics) ,Stoichiometry ,Arsenic - Abstract
Using pulsed laser ablation with arsenic over pressure, the growth conditions for GaAs nanowires have been systematically investigated and optimized. Arsenic over pressure with As$_2$ molecules was introduced to the system by thermal decomposition of polycrystalline GaAs to control the stoichiometry and shape of the nanowires during growth. GaAs nanowires exhibit a variety of geometries under varying arsenic over pressure, which can be understood by different growth processes via vapor-liquid-solid mechanism. Single-crystal GaAs nanowires with uniform diameter, lengths over 20 $\mu$m, and thin surface oxide layer were obtained and can potentially be used for further electronic characterization.
- Published
- 2007
- Full Text
- View/download PDF
34. First Report of Streptomyces acidiscabies Causing White Radish Scab in China
- Author
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D. J. Yang, Xiu-Mei Yu, Weiquan Zhao, Daqun Liu, and F. Y. Yang
- Subjects
Genetics ,White (mutation) ,Streptomyces acidiscabies ,Botany ,Morphology (biology) ,Plant Science ,Ribosomal RNA ,Biology ,biology.organism_classification ,Pathogenicity ,Agronomy and Crop Science ,Gene ,DNA sequencing - Published
- 2015
35. Reconstruction control of magnetic properties during epitaxial growth of ferromagnetic Mn3-deltaGa on Wurtzite GaN(0001)
- Author
-
Erdong, Lu, David C, Ingram, Arthur R, Smith, J W, Knepper, and F Y, Yang
- Abstract
Binary ferromagnetic Mn(3-delta)Ga (1.23-deltaor =1.5) crystalline thin films have been epitaxially grown on wurtzite GaN(0001) surfaces using rf N-plasma molecular beam epitaxy. The film structure is face-centered tetragonal with CuAu type-I (L1(0)) ordering with (111) orientation. The in-plane epitaxial relationship to GaN is nearly ideal with [110](MnGa) parallel[1100](GaN) and [112](MnGa) parallel[1120](GaN). We observe magnetic anisotropy along both the in-plane and out-of-plane directions. The magnetic moments are found to depend on the Mn/(Mn+Ga) flux ratio and can be controlled by observation of the surface reconstruction during growth, which varies from 1x1 to 2x2 with increasing Mn stoichiometry.
- Published
- 2006
36. Stable Polymer Dielectric Film for P3HT TFT by Modified Poly-(Vinyl Phenol) with Polar Functional Group
- Author
-
Po-Yuan Lo, Y. C. Lin, F. Y. Yang, Zingway Pei, Y. R. Peng, and Yi-Jen Chan
- Subjects
chemistry.chemical_compound ,Materials science ,Polymer dielectric ,chemistry ,Thin-film transistor ,Functional group ,Polymer chemistry ,Phenol ,Polar - Published
- 2006
37. Synergistic effects of glycated chitosan with high-intensity focused ultrasound on suppression of metastases in a syngeneic breast tumor model
- Author
-
F. Y. Yang, Y. L. Chen, Liang-ting Lin, S. J. Chiu, Yi Jang Lee, C. Y. Wang, F. D. Chen, J. D. Leu, B. S. Wang, J. Y. Chen, and W. R. Chen
- Subjects
Diagnostic Imaging ,Cancer Research ,Pathology ,medicine.medical_specialty ,Epithelial-Mesenchymal Transition ,Immunology ,Breast Neoplasms ,Biology ,epithelial–mesenchymal transition ,Transfection ,Immunoadjuvant ,Metastasis ,Cellular and Molecular Neuroscience ,breast cancer ,Immune system ,Breast cancer ,Cell Movement ,Genes, Reporter ,In vivo ,Cell Line, Tumor ,Biomarkers, Tumor ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Epithelial–mesenchymal transition ,Neoplasm Metastasis ,Cytotoxicity ,high-intensity focused ultrasound ,Chitosan ,Mice, Inbred BALB C ,Cell Death ,lung metastasis ,Macrophages ,Cell Biology ,medicine.disease ,Disease Models, Animal ,Cancer cell ,immunoadjuvant ,Cancer research ,High-Intensity Focused Ultrasound Ablation ,Original Article ,Female - Abstract
Stimulation of the host immune system is crucial in cancer treatment. In particular, nonspecific immunotherapies, when combined with other traditional therapies such as radiation and chemotherapy, may induce immunity against primary and metastatic tumors. In this study, we demonstrate that a novel, non-toxic immunoadjuvant, glycated chitosan (GC), decreases the motility and invasion of mammalian breast cancer cells in vitro and in vivo. Lung metastatic ratios were reduced in 4T1 tumor-bearing mice when intratumoral GC injection was combined with local high-intensity focused ultrasound (HIFU) treatment. We postulate that this treatment modality stimulates the host immune system to combat cancer cells, as macrophage accumulation in tumor lesions was detected after GC-HIFU treatment. In addition, plasma collected from GC-HIFU-treated tumor-bearing mice exhibited tumor-specific cytotoxicity. We also investigated the effect of GC on epithelial-mesenchymal transition-related markers. Our results showed that GC decreased the expression of Twist-1 and Slug, proto-oncogenes commonly implicated in metastasis. Epithelial-cadherin, which is regulated by these genes, was also upregulated. Taken together, our current data suggest that GC alone can reduce cancer cell motility and invasion, whereas GC-HIFU treatment can induce immune responses to suppress tumor metastasis in vivo.
- Published
- 2014
38. Determination of the spin polarization of half-metallic CrO(2) by point contact Andreev reflection
- Author
-
Y, Ji, G J, Strijkers, F Y, Yang, C L, Chien, J M, Byers, A, Anguelouch, G, Xiao, and A, Gupta
- Abstract
Andreev reflection at a Pb/CrO(2) point contact has been used to determine the spin polarization of single-crystal CrO(2) films made by chemical vapor deposition. The spin polarization is found to be 0.96 +/- 0.01, which confirms that CrO(2) is a half-metallic ferromagnet, as theoretically predicted.
- Published
- 2000
39. An application of video‐camera and PC‐oriented image processor to an experiment on the coherent property of light
- Author
-
Y. I. Chang, I. C. Huang, P. K. Tseng, J. R. Wang, T. C. Fang, F. Y. Yang, and S. F. Tsai
- Subjects
Physics ,Property (programming) ,Image processor ,business.industry ,Scattering ,General Physics and Astronomy ,Image processing ,Video camera ,Interference (wave propagation) ,Electromagnetic radiation ,law.invention ,Optics ,law ,Professional video camera ,business - Published
- 1990
40. Transmembrane Ca2+ gradient is essential for high anion transport activity of human erythrocytes
- Author
-
C. Feng, Z. Y. Guang, Yaping Tu, Q. W. Lu, F. Y. Yang, and H. Xu
- Subjects
Anions ,biology ,Chemistry ,Transport activity ,Protein Conformation ,Erythrocyte Membrane ,Biophysics ,Ionophore ,Biological Transport ,Cell Biology ,Biochemistry ,Transmembrane protein ,Ion ,Crystallography ,Membrane ,Erythrocyte Ghosts ,Anion Exchange Protein 1, Erythrocyte ,biology.protein ,Human erythrocytes ,Humans ,Calcium ,Molecular Biology ,Band 3 - Abstract
The role of a transmembrane Ca2+ gradient in anion transport by Band 3 of human resealed erythrocyte ghosts has been studied. The results show that a transmembrane Ca2+ gradient is essential for the conformation of erythrocyte Band 3 with higher anion transport activity. The dissipation of the transmembrane Ca2+ gradient by the ionophore A23187 inhibits the anion transport activity. The extent of this inhibition approaches 90% as the Ca2+ concentration on both sides of the ghost membrane is increased to 1.0 mM and half-maximum inhibition is observed at 0.25 mM Ca2+. Addition of ATP (0.4 mM) to the resealing medium can partly reestablish the transmembrane Ca2+ gradient by activation of Ca2+-ATPase and alleviate the inhibition to some extent. N-ethylmaleimide, an inhibitor of erythrocyte Ca2+-ATPase, prevents such restoration. Electron micrographs reveal that numerous larger intramembranous particles can be observed on the P-faces of freeze-fractured resealed ghosts in the absence of a transmembrane Ca2+ gradient.
- Published
- 1996
41. V92A mutation altered the folding propensity of chicken apocytochrome c and its interaction with phospholipids
- Author
-
F Y Yang, Tong Jc, and L Q Zhu
- Subjects
Circular dichroism ,Protein Denaturation ,Protein Folding ,Protein Conformation ,Lipoproteins ,Mutant ,Molecular Sequence Data ,Gene Expression ,Cytochrome c Group ,Biochemistry ,Protein structure ,Valine ,Escherichia coli ,Animals ,Trypsin ,Lipid bilayer ,Phospholipids ,DNA Primers ,chemistry.chemical_classification ,Perchlorates ,biology ,Base Sequence ,Cytochrome c ,Circular Dichroism ,Titrimetry ,Cytochromes c ,Trifluoroethanol ,Sodium Compounds ,Amino acid ,chemistry ,Spectrophotometry ,biology.protein ,Mutagenesis, Site-Directed ,Protein folding ,Apoproteins ,Chickens - Abstract
Chicken apocytochrome c has been shown to possess a much stronger tendency to fold spontaneously in aqueous solution than the equivalent enzyme from other species. In the present work, the amino acid that determines its folding ability was elucidated by site-directed mutagenesis. Wild-type chicken apocytochrome c and three mutants V92A, S103A, and V92A/S103A were expressed in Escherichia coli. The wild-type apoprotein and S103A exhibited the same folding property during dialysis renaturation processes as that chemically prepared from chicken cytochrome c, while those containing V92A mutation did not. Quantitative studies by 2,2,2-trifluoroethanol (TFE) and sodium perchlorate (NaClO4) titration demonstrated that the V92A mutation decreased the helix content that could be induced and confirmed that valine 92 is the major determinant of the folding propensity of chicken apocytochrome c. Furthermore, CD spectra, turbidity measurements, and a translocation assay on a model membrane system showed that the V92A mutation also drastically altered the conformation of apocytochrome c after being incorporated into lipid bilayer and decreased the aggregation of phospholipid vesicles after association of the apoprotein, thus rendering the molecule more competent for translocation across the membrane. Our results showed that a single amino acid substitution could radically alter the folding propensity of an unfolded polypeptide chain and thus influence the conformation following its insertion into phospholipid bilayer.
- Published
- 1996
42. First Report of Sclerotinia Rot of Chinese Atractylodes Caused by Sclerotinia nivalis in China
- Author
-
J. F. Fu, Z.-H. Liu, F. Y. Yang, Rujun Zhou, and Haijiao Xu
- Subjects
biology ,Inoculation ,fungi ,food and beverages ,Plant Science ,Fungus ,biology.organism_classification ,Rhizome ,Botany ,Atractylodes ,Potato dextrose agar ,Pith ,Agronomy and Crop Science ,Sclerotinia ,Mycelium - Abstract
Chinese atractylodes (Atractylodes japonica Koidz.ez Kitam.) is a perennial herb in the Compositae family, and is widely distributed in China. The dried rhizomes of the plant are used in traditional Chinese medicine. During the summer of 2011, typical signs and symptoms of Sclerotinia rot were observed on Chinese atractylodes in a production field of Liaoning Province of China. Symptoms were observed in plants at the flowering stage, distributed in patches throughout the rows, and with a disease incidence of approximately 10 to 15%. The lower mature leaves of infected plants first became yellow and wilted, basal stem areas showed a black-brown rot at the same time under conditions of high humidity, and white cottony mycelium formed along the basal stem and soil surfaces. Ultimately, the basal stem and roots rotted and the plants wilted and died quickly. Black, irregular sclerotia (average 0.8 to 6.9 mm in diameter) were also observed within the pith cavity of split stems and rotted roots. The pathogen was isolated from symptomatic tissues and sclerotia, surface disinfested with 2% sodium hypochlorite, and cultured on potato dextrose agar (PDA) (1). The fungus was mesophilic, with an optimum temperature for mycelial growth in culture of about 20°C. Colonies on PDA produced masses of white aerial mycelium, with small white flocci distributed among sclerotia. After 2 weeks, sclerotia 0.5 to 4.5 mm in diameter were produced near the margin in a uniform distribution. Sclerotia were spherical, elongated, or fused to form irregular shapes and tightly attached to the agar surface by their under surface, which could be seen through the bottom of the petri dishes. DNA sequences of five replicates were obtained using the TianGen DNA secure plant kit. The internal transcribed spacer (ITS) region of rDNA was amplified with primers ITS1/ITS4 and sequenced. BLAST analysis of the 513-bp segment showed high similarity (99%) with a sequence of Sclerotinia nivalis (GenBank Accession No. AB516670). A representative sequence was deposited in GenBank (Accession No. JX294862). The fungus isolated from symptomatic tissues was identified as S. nivalis Saito on the basis of morphological and cultural characteristics (2,3) and ITS sequence analysis. Symptoms were reproduced in the greenhouse by inoculating the basal stem and roots of 15 atractylodes plants at the 7- to 10-leaf stage. Inoculum was prepared by macerating 14-day-old PDA cultures of the fungus in a blender and placing the mixture (approximately 20 g) into the potting medium of each plant. Sterile PDA was used to inoculate the five control plants. Plants were maintained in a greenhouse at 22 to 25°C and about 75% relative humidity. After 7 to 10 days, symptoms were similar to those in the fields. Lower leaves of inoculated plants became yellow and wilted, and infected plants died 2 weeks after inoculation, whereas control plants remained healthy. The pathogen was successfully recovered from symptomatic tissues, completing Koch's postulates. To our knowledge, this is the first report of Sclerotinia rot of Chinese atractylodes. Given its wide host range, S. nivalis has great potential to become an economically important plant pathogen. References: (1) W. G. Kim and W. D. Cho. Mycobiology 30:41, 2002. (2) G. Q. Li et al. Mycol. Res. 104:232, 2000. (3) I. Saito. Mycoscience 38:227, 1997.
- Published
- 2012
43. Cloning and high-level expression of chicken apocytochrome c gene in Escherichia coli
- Author
-
J C, Tong, L Q, Zhu, and F Y, Yang
- Subjects
Reading Frames ,Base Sequence ,Molecular Sequence Data ,Restriction Mapping ,Cytochromes c ,Cytochrome c Group ,Polymerase Chain Reaction ,Introns ,Recombinant Proteins ,Escherichia coli ,Animals ,Cloning, Molecular ,Apoproteins ,Chickens ,Plasmids - Abstract
Chicken apocytochrome c gene with correct reading frame was easily cloned through excision by polymerase chain reaction of the intron in the genomic clone of chicken cytochrome c gene, and was successfully overexpressed in Escherichia coli by cloning into expression vector pET-3d under the control of T7 promoter. Expressed protein can amount to as high as 40% of the total protein and mainly presents as inclusion body. Purification of chicken apocytochrome c from the inclusion body and characterization by SDS-PAGE, isoelectric focusing electrophoresis, and amino acid analysis showed that the purified apocytochrome c is identical to that prepared from chicken heart cytochrome c by chemically depletion of heme.
- Published
- 1995
44. Hydrophobic interaction and folding propensity of chicken heart apocytochrome c
- Author
-
J C, Tong, P, Yi, and F Y, Yang
- Subjects
Protein Folding ,Macromolecular Substances ,Protein Conformation ,Circular Dichroism ,Osmolar Concentration ,Cytochromes c ,Cytochrome c Group ,Hydrogen-Ion Concentration ,Mitochondria, Heart ,Kinetics ,Spectrometry, Fluorescence ,Animals ,Electrophoresis, Polyacrylamide Gel ,Horses ,Apoproteins ,Chickens - Abstract
In contrast to horse heart apocytochrome c, the chicken one showed quite different folding propensity as titrated by NaCl at different pH. At pH 2.0, folding behaviour of both apocytochrome c are essentially similar; while at pH higher than 4.0, chicken heart apocytochrome c has much enhanced propensity to fold and aggregate, as was shown by circular dichroism spectra, intrinsic fluorescence and non-denatured polyacrylamide gel electrophoresis. Hydrophobic chromatography demonstrated much higher hydrophobicity of chicken heart apocytochrome c, thus strongly suggested that it is the hydrophobic interaction that stabilize the 'Molten Globule' like, partially-folded structure of chicken heart apocytochrome c at neutral pH.
- Published
- 1995
45. Zn(2+)-mediated domain-domain communication in human erythrocyte band 3
- Author
-
Y P, Tu and F Y, Yang
- Subjects
Cytoplasm ,Zinc ,Binding Sites ,Anion Exchange Protein 1, Erythrocyte ,Erythrocyte Membrane ,Liposomes ,Humans ,Fluorometry ,Trypsin ,Protein Structure, Tertiary - Abstract
Zn2+ could inhibit the anion transport activity of spectrin-stripped inside-out human erythrocyte membrane vesicles (IOVs). Removal of the cytoplasmic domain from Band 3 by trypsin could eliminate Zn2+ inhibition. The location of a Zn(2+)-binding site was confirmed by atomic absorbance spectrometry. The results of time-resolved fluorescence and intrinsic fluorescence quenching by KI and hypocrellin B (a photosensitive pigment obtained from a parasitic fungus growing in Yunnan, China) showed that the cytoplasmic domain is necessary for the Zn(2+)-induced conformational changes of the whole molecule as well as the membrane domain of Band 3. It is suggested that Zn2+ induced a conformational change in the cytoplasmic domain of Band 3, which in turn was transmitted to the membrane domain, resulting in an inhibition of activity of Band 3. Such long-range conformational changes may imply that the cytoplasmic domain is poised to function as a cytosolic arm in order to modulate the structure of the membrane domain of Band 3.
- Published
- 1995
46. Cytoplasmic Ca2+ inhibits the glucose transporter of human erythrocytes
- Author
-
Y P, Tu, L, Xiao, X F, Su, and F Y, Yang
- Subjects
Cytoplasm ,Erythrocytes ,Glucose ,Monosaccharide Transport Proteins ,Cytochalasin B ,Mercuric Chloride ,Humans ,Calcium ,Magnesium ,Egtazic Acid ,Calcimycin - Abstract
The effect of Ca2+ on the glucose transporter of human erythrocytes was investigated. The results showed that extracellular Ca2+ had no effect. But, the glucose transport of erythrocytes was markedly inhibited due to the increase in cytoplasmic Ca2+ concentration by addition of ionophore A23187. The Ca2+ inhibition exhibited a dose-dependent manner with an apparent half maximal concentration of 250 microM and could not be recovered by 10 mM EGTA. Unlike Ca2+, Mg2+ did not affect the glucose transporter.
- Published
- 1995
47. Correlation between unfolded states of apocytochrome c and its ability to pass lipid bilayer
- Author
-
X S, Wang, J C, Tong, X H, Han, and F Y, Yang
- Subjects
Protein Folding ,Lipid Bilayers ,Molecular Sequence Data ,Nucleic Acid Renaturation ,Animals ,Cytochromes c ,Cytochrome c Group ,Amino Acid Sequence ,Horses ,Apoproteins ,Chickens ,Phospholipids - Abstract
In contrast to the horse heart apocytochrome c, the chicken heart apocytochrome c underwent a conformational change from random coil to partial folding during a renaturation process. When the apocytochrome horse heart and that of chicken heart c were subjected to a translocation assay in vitro using large trypsin-enclosed unilamellar vesicles from soybean phospholipids, the ability of the chicken heart apocytochrome c to penetrate into the liposomes was found to decrease markedly with the renaturation procedure, while that of horse heart apocytochrome c remained relatively constant. Observations from circular dichroism measurement on the induction of secondary folding of these two species of apocytochrome c upon interaction with soybean phospholipid vesicles suggested that a more flexible structure of apocytochrome c embedded in the lipid matrix be required for its efficient translocation across the bilayer.
- Published
- 1994
48. Transmembrane Ca2+ gradient-mediated change of fluidity in the inner layer of phospholipids modulates Ca(2+)-ATPase of sarcoplasmic reticulum
- Author
-
Y P, Tu, H, Xu, and F Y, Yang
- Subjects
Sarcoplasmic Reticulum ,Membrane Fluidity ,Cell Membrane ,Lipid Bilayers ,Animals ,Calcium ,Calcium-Transporting ATPases ,Intracellular Membranes ,Rabbits ,Phospholipids ,Fluorescent Dyes - Abstract
Sarcoplasmic reticulum (SR) vesicles with (1000 folds) or without transmembrane Ca2+ gradient have been prepared. Different fluorescence probes (DPH, TMA-DPH and n-AS), were used to determine the effect of transmembrane Ca2+ gradient on the lipid fluidity both in outer and inner layer of Ca(2+)-ATPase-containing SR vesicles. The results showed that transmembrane Ca2+ gradient could significantly decrease the fluidity of the inner layer of SR membrane, while no obvious change was monitored in the outer layer. This may be deduced that Ca(2+)-ATPase might be modulated mainly by the transmembrane Ca2+ gradient-mediated alteration of physical state of phospholipid in the inner layer of SR membrane.
- Published
- 1994
49. [The specificity of modulation of sarcoplasmic reticulum Ca(2+)-ATPase by transmembrane Ca2+ gradient]
- Author
-
Y P, Tu, H, Xu, and F Y, Yang
- Subjects
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone ,Sarcoplasmic Reticulum ,Strontium ,Animals ,Calcium ,Calcium-Transporting ATPases ,Rabbits ,Calcimycin ,Membrane Potentials - Abstract
We have previously reported that transmembrane Ca2+ gradient-mediated changes in lipid fluidity could modulate the conformation and enzyme activity of sarcoplasmic reticulum (SR) Ca(2+)-ATPase. The aim of this paper is to explore the specificity of transmembrane Ca2+ gradient-mediated modulation of SR Ca(2+)-ATPase. The results showed that such specificity exhibited in two aspects: 1. The modulation could not be ascribed to transmembrane potential resulted from the transmembrane Ca2+ gradient, Dissipation of transmembrane potential by FCCP (carbonylcyanide-p-trifluoromethoxyphenylhydrazone) could not affect the activity of SR Ca(2+)-ATPase. 2. Transmembrane Sr2+ gradient had little effect on the enzyme activity of SR Ca(2+)-ATPase. A significant difference between the effect of transmembrane Ca2+ and Sr2+ gradient on the lipid fluidity was detected in the middle region of bilayer of Ca(2+)-ATPase incorporated proteoliposomes using a set of n-AS [n-(9-anthroyloxy) fatty acids] fluorescence polarization probes. It is known that Ca2+ binding domain of SR Ca(2+)-ATPase is just located in the middle region of bilayer, hence it may be deduced that possibly, membrane lipids are involved in transmembrane Ca2+ gradient-mediated modulation of Ca(2+)-ATPase.
- Published
- 1993
50. Study on the translocation of chicken heart apocytochrome C with different unfolded states
- Author
-
F Y, Yang, X S, Wang, J C, Tong, and X H, Han
- Subjects
Protein Folding ,Protein Conformation ,Tuna ,Circular Dichroism ,Myocardium ,Lipid Bilayers ,Cytochromes c ,Cytochrome c Group ,Liposomes ,Animals ,Spectrophotometry, Ultraviolet ,Horses ,Apoproteins ,Chickens ,Candida - Abstract
Chemically-prepared chicken heart apocytochrome c with different unfolded states could be obtained during the renaturation process. They exhibited distinct circular dichroism patterns designated as Apo C1 (random coiled), Apo C2 (less ordered) and Apo C3 (more ordered). This characteristic is unique to chicken heart apocytochrome c while compared with its counterparts from Candida krusei, tuna heart or horse heart and promises the emergence of much more detail of correlation of translocation with unfolded states of apocytochrome c. When chicken heart apocytochrome c was subjected to a translocation assay in vitro using trypsin-enclosed large unilamellar vesicles from soybean phospholipids, the ability of the protein to penetrate into the liposomes was found to follow the order of Apo C1Apo C2Apo C3. Conformational alterations of Apo C1, Apo C2 and Apo C3 in association with soybean phospholipid vesicles shown by circular dichroism measurement demonstrated that Apo C1 bound to phospholipids existed in a more loosely folded conformation than Apo C2 and Apo C3. We propose that a more flexible structure of apocytochrome c following the interaction with phospholipids is required for its efficient translocation across the bilayer.
- Published
- 1993
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