1. Preparation of protein samples for gel electrophoresis by sequential extraction
- Author
-
Zhong Bo-xiong, Weng Hong-biao, and Fang Wei-huan
- Subjects
Gel electrophoresis ,Chromatography ,Two-dimensional gel electrophoresis ,biology ,Chemistry ,fungi ,Extraction (chemistry) ,General Engineering ,Bioinformatics ,biology.organism_classification ,Solubilization ,Bombyx mori ,Proteome ,Extraction methods ,Peptide sequence - Abstract
Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm,Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about 15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with the sequential extraction method; which will be useful for preparation of protein samples for proteome study.
- Published
- 2002