1. MiR-153-3p reduces extracellular matrix accumulation in high glucose-stimulated human glomerular mesangial cells via targeting PAQR3 in diabetic nephropathy
- Author
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Yao Wubin, Chen Dongmei, Fang Xingxing, Yang Hongli, Shen Lianglan, and Shen Yan
- Subjects
Nutrition and Dietetics ,Mesangial cell ,Cell growth ,Chemistry ,Endocrinology, Diabetes and Metabolism ,Glomerular Mesangial Cell ,Transfection ,Extracellular Matrix ,Cell biology ,Extracellular matrix ,MicroRNAs ,Phosphatidylinositol 3-Kinases ,Glucose ,Endocrinology ,Mesangial Cells ,Diabetes Mellitus ,Humans ,Diabetic Nephropathies ,MTT assay ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Signal Transduction - Abstract
Introduction This study aims to explore the effect and related molecular mechanism of miR-153-3p on high glucose-stimulated human glomerular mesangial cells. Materials and methods The quantitative real-time polymerase chain reaction (qPCR) assay was employed to check miR-153-3p and PAQR3 expression levels in diabetic nephropathy patients. (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT assay was applied to investigate the effects of miR-153-3p transfection or PAQR3 administration on mesangial cell (MC) activity. ELISA assays were used to check the expression levels of extracellular matrix (ECM) related proteins. The bioinformatics method and dual-luciferase reporter assay were employed together to anticipate and check the targeting relationship between miR-153-3p and PAQR3. Western blot assays were applied to check the PAQR3, PI3K and AKT expression after miR-153-3p transfection or PAQR3 administration. Results The expression level of miR-153-3p was lower in diabetic nephropathy patients, while the expression of PAQR3 was concomitantly higher. Upregulation of miR-153-3p can reduce MC proliferation and ECM accumulation. Further research indicated that miR-153-3p directly regulated PAQR3 expression via coupling with the 3’-UTR of PAQR3. Finally, the fact that miR-153-3p regulates the PI3K/AKT pathway by PAQR3 was confirmed. Conclusion MiR-153-3p regulates the PI3K/AKT pathway through PAQR3, thereby playing a role in regulating cell proliferation and ECM accumulation in high glucose-stimulated MCs.
- Published
- 2022