1. A capillary electrophoretic system based on a novel microemulsion for the analysis of coenzyme Q10 in human plasma by electrokinetic chromatography
- Author
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Silvia, Lucangioli, Sabrina, Flor, Flor, Sabrina, Mario, Contin, Contin, Mario, Valeria, Tripodi, and Tripodi, Valeria
- Subjects
Adult ,Male ,Accuracy and precision ,Ubiquinone ,Capillary action ,Butanols ,Clinical Biochemistry ,Analytical chemistry ,Sensitivity and Specificity ,Biochemistry ,Analytical Chemistry ,Bile Acids and Salts ,Surface-Active Agents ,chemistry.chemical_compound ,Humans ,Microemulsion ,Chromatography, High Pressure Liquid ,Chromatography, Micellar Electrokinetic Capillary ,Octane ,Chromatography ,Butanol ,Temperature ,Electrophoresis, Capillary ,Reproducibility of Results ,Succinates ,Hydrogen-Ion Concentration ,Octanes ,Hexane ,Electrophoresis ,chemistry ,Linear Models ,Emulsions ,Female ,Steroids ,Selectivity - Abstract
A new analytical method for determination of coenzyme Q10 (2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone, CoQ10) in human plasma was developed based on CE using a double tensioactive microemulsion. CoQ10 was quantitatively extracted into 1-propanol/hexane and quantified by MEEKC. The microemulsion was prepared by mixing 1.4% w/w sodium bis(2-ethylhexyl) sulfosuccinate, 4% w/w cholic acid, 1% w/w octane, 8.5% w/w butanol, 0.1% w/w PVA and 85% w/w 10 mM Tris buffer at pH 9.0. The optimized electrophoretic conditions included the use of an uncoated silica capillary of 60 cm total length and 75 mum id, an applied voltage of 20 kV, room temperature and 214 nm ultraviolet detection. Selectivity, linearity, LOD, LOQ, precision and accuracy were evaluated as the parameters of validation. Owing to its simplicity and reliability, the proposed method can be an advantageous alternative to the traditional methodology for the quantitation of CoQ10 in human plasma with good accuracy and precision.
- Published
- 2009
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