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Coordinated effects of glucose and oleic acid on glucagon-like peptide-1 (GLP-1) mediated differentiation of insulin-positive differentiating umbilical cord mesenchymal stromal cells (dUCBMSCs) was studied using a co-culture of NCI-H716 (GLP-1+) and UCBMSCs (insulin+). The addition of 2.5 mM glucose increased the proliferation of NCI-H716 cells by 30% and induced transformation of UCBMSCs into insulin-secreting cells in 18 days as compared to 22 days in control cells. Oleic acid (25 μM) showed decrease in cell proliferation, autophagy, and apoptosis in NCI-H716 cells while no effect was observed in dUCBMSCs. Prolonged glucose and oleic acid resulted in apoptosis and cell cycle changes in dUCBMSCs after day 18 while higher concentrations resulted in cell death. Additionally, the expression of FAS and ACC mRNA was observed in NCI-H716 and dUCBMSCs post 24-hr addition of glucose and/or oleic acid. Absorption of oleic acid was high in NCI-H716 compared to dUCBMSCs. Taken together, optimal concentrations of glucose and oleic acid could be a key factor in stimulating intrinsic GLP-1, which in turn stimulates differentiating MSCs in a glucose-dependent manner. PRACTICAL APPLICATIONS: The aim of this article was to study whether differentiating or differentiated MSCs after mobilization or post-transplant would require optimal glucose and oleic acid to naturally stimulate intrinsic GLP-1, or otherwise, the high or long-term overload of glucose or oleic acid could result in inhibition of differentiated cells resulting in failure of insulin secretion.

A mathematical study is presented to analyze the nonlinear, non-isothermal, magnetohydrodynamic (MHD) free convection boundary layer flow, heat and mass transfer of non-Newtonian Eyring–Powell fluid from a vertical surface in a non-Darcy, isotropic, homogenous porous medium, in the presence of Hall currents and ionslip currents. The governing nonlinear coupled partial differential equations for momentum conservation in the x, and z directions, heat and mass conservation, in the flow regime are transformed from an (x, y, z) coordinate system to a (ξ, η) coordinate system in terms of dimensionless x-direction velocity (f′) and z-direction velocity (G), dimensionless temperature and concentration functions (θ and ϕ) under appropriate boundary conditions. Both Darcian and Forchheimer porous impedances are incorporated in both momentum equations. Computations are also provided for the variation of the x and z direction shear stress components and also heat and mass transfer rates. It is observed that with increasing ɛ, primary velocity, secondary velocity, heat and mass transfer rates are decreased whereas, the temperature, concentration and skin friction are increased. An increasing δ is found to increase primary and secondary velocities, skin friction, heat and mass transfer rates. But the temperature and concentration decrease. Increasing βe and βi are seen to increase primary velocity, skin friction, heat and mass transfer rates whereas secondary velocity, temperature and concentration are decreased. Excellent correlation is achieved with a Nakamura tridiagonal finite difference scheme (NTM). The model finds applications in magnetic materials processing, MHD power generators and purification of crude oils.