1. Detection of high mobility group A2 specific mRNA in the plasma of patients affected by epithelial ovarian cancer
- Author
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Cono Scaffa, Anna Maria Bello, Emilia Vuttariello, Daniela Califano, Celeste Calise, Stefano Greggi, Rosa Pasquinelli, Gennaro Chiappetta, Nunzia Simona Losito, A Romano, Carmela Pisano, Alfredo Fusco, Francesca Galdiero, Sandro Pignata, Galdiero, Francesca, Romano, Annunciata, Pasquinelli, Rosa, Pignata, Sandro, Greggi, Stefano, Vuttariello, Emilia, Bello, Anna Maria, Calise, Celeste, Scaffa, Cono, Pisano, Carmela, Losito, Nunzia Simona, Fusco, Alfredo, Califano, Daniela, and Chiappetta, Gennaro
- Subjects
Adult ,medicine.medical_specialty ,HMGA2 ,endocrine system diseases ,Carcinoma, Ovarian Epithelial ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Humans ,Epithelial ovarian cancer ,Neoplasms, Glandular and Epithelial ,RNA, Messenger ,plasma ,Aged ,Gynecology ,Ovarian Neoplasms ,business.industry ,HMGA2 Protein ,Middle Aged ,Circulating RNA ,female genital diseases and pregnancy complications ,Ovarian Cancer ,Oncology ,Gynecological malignancy ,Immunology ,Ovarian carcinomas ,Female ,circulating RNA ,Clinical Research Paper ,business - Abstract
// Francesca Galdiero 1, * , Annunciata Romano 1, * , Rosa Pasquinelli 1 , Sandro Pignata 2 , Stefano Greggi 2 , Emilia Vuttariello 1 , Anna Maria Bello 1 , Celeste Calise 1 , Cono Scaffa 2 , Carmela Pisano 2 , Nunzia Simona Losito 3 , Alfredo Fusco 4 , Daniela Califano 1 , Gennaro Chiappetta 1 1 Genomica Funzionale, Istituto Nazionale per lo Studio e la Cura dei Tumori “Fondazione Giovanni Pascale”, IRCCS, Naples, Italy 2 Dipartimento di Oncologia Uroginecologica, Istituto Nazionale per lo Studio e la Cura dei Tumori “Fondazione Giovanni Pascale”, IRCCS, Naples, Italy 3 Anatomia Patologica, Istituto Nazionale per lo Studio e la Cura dei Tumori “Fondazione Giovanni Pascale”, IRCCS, Naples, Italy 4 Istituto di Endocrinologia ed Oncologia Sperimentale - CNR Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universita degli Studi di Napoli Federico II, Naples, Italy * These authors have contributed equally to this work Correspondence to: Daniela Califano, e-mail: d.califano@istitutotumori.na.it Keywords: Ovarian Cancer, HMGA2, plasma, circulating RNA Received: July 25, 2014 Accepted: December 15, 2014 Published: February 12, 2015 ABSTRACT Ovarian cancer is the most lethal gynecological malignancy and the high mortality rate is associated with advanced-stage disease at the time of the diagnosis. In order to find new tools to make diagnosis of Epithelial Ovarian Cancer (EOC) at early stages we have analyzed the presence of specific HMGA2 mRNA in the plasma of patients affected by this neoplasm. HMGA2 overexpression represents a feature of several malignances including ovarian carcinomas. Notably, we detected HMGA2 specific mRNA in the plasma of 40 out 47 patients with EOC, but not in the plasma of healthy donors. All cases found positive for HMGA2 mRNA in the plasma showed HMGA2 protein expression in EOC tissues. Therefore, on the basis of these results, the analysis of circulating HMGA2 specific mRNA might be considered a very promising tool for the early diagnosis of EOC.
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- 2014