1. Lingaoamide, a cyclic heptapeptide from a Chinese freshwater cyanobacterium Oscillatoria sp
- Author
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C. Benjamin Naman, Xiaojun Yan, Kiyotake Suenaga, Arihiro Iwasaki, Haixi Luo, Chengcong Zhu, Naoaki Kurisawa, Shan He, Tingting Wang, Xiaohui Li, Gaurav Patial, Fuli Tian, and Tal Luzzatto-Knaan
- Subjects
Cyanobacteria ,Oscillatoria ,biology ,010405 organic chemistry ,Bacillus pumilus ,Chemistry ,fungi ,Organic Chemistry ,food and beverages ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,Biochemistry ,In vitro ,0104 chemical sciences ,Melanin ,Drug Discovery ,Spinach ,Arabidopsis thaliana ,Magnaporthe grisea - Abstract
Lingaoamide (1), a new cyclic heptapeptide natural product, was isolated from a freshwater Oscillatoria cyanobacterium. The producing organism was collected in Chinese water spinach paddy farm fields in Lingao County, Hainan, China. The structure of 1 was determined by conventional mass spectrometry, NMR spectroscopy, and chemical degradation studies. Lingaoamide concentration-dependently up-regulates the biogenesis of pro-pathogenic melanin pigments (melanogenesis) in the rice blast fungus, Magnaporthe grisea, when tested in vitro. This molecule also exhibits weak in vitro inhibition (MIC 32 μg/mL) of Gram-negative Pseudomonas aeruginosa and Gram-positive Bacillus pumilus, and does not appear to impact plant growth or immune response in vivo using the model organism, Arabidopsis thaliana.
- Published
- 2021
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