1. Proteomic analysis of knock-down HLA-G in invasion of human trophoblast cell line JEG-3
- Author
-
Liu, Haiyan, Liu, Xueyuan, Jin, Hong, Yang, Fengying, Gu, Weirong, and Li, Xiaotian
- Subjects
HLA-G Antigens ,Proteomics ,Blotting, Western ,Eukaryotic Initiation Factor-2 ,Down-Regulation ,Transfection ,Cell Line ,Trophoblasts ,Autocrine Communication ,Matrix Metalloproteinase 9 ,Cell Movement ,Malate Dehydrogenase ,Pregnancy ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Humans ,Matrix Metalloproteinase 2 ,Original Article ,Electrophoresis, Gel, Two-Dimensional ,Female ,RNA Interference ,RNA, Messenger ,Molecular Chaperones - Abstract
Previous studies showed that aberrant HLA-G expression in trophoblast cells plays important roles in trophoblast invasion; however, the mechanisms remain to be explored. In this study, we found that suppressed HLA-G expression could dramatically decrease the mRNA and protein expression levels of matrix metalloproteinase 2 and matrix metalloproteinase 9, and in the proteome assay, there were 3 identified proteins namely, prefoldin 1, eukaryotic translation elongation factor 2 and malate dehydrogenase 2, which were verified by Western blot and known to be associated with invasion, cell cycle and cell metabolism, respectively. Collectively, our study indicated a potential involvement of HLA-G in autocrine networks that may regulate prefoldin, MMPs and trophoblast invasion at the maternal-fetal interface in human pregnancy.