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Background: CRF01_AE and CRF07_BC are the two major HIV-1 virus strains circulating in China. The proportion of dominant subtypes (CRF01_AE and CRF07_BC) among MSM in Jiangsu province was over 80%. A large number of URFs have been found in China in recent years. Objective: This study aimed to report on novel HIV-1 recombinants. Method: We constructed Phylogenetic trees using the maximum likelihood (ML) method with 1000 bootstrap replicates in IQ-TREE 1.6.8 software and determined recombination breakpoints using SimPlot 3.5.1. Results: We identified a novel, second-generation HIV-1 recombinant (JS020202) between CRF01_ AE and CRF07_BC. The analysis of near full-length genome (NFLG) showed there were at least 8 breakpoints in the virus, which differed from any previously identified CRF and URF around the world. Conclusion: Novel diverse CRF01_AE/07_BC suggested the complexity trends of HIV-1 genetics. The emergency situation of diverse recombinant strains should be monitored continuously.

Additional file 1. Breakfast Survey on March 2 (Lantern Festival)

Background: Circulating miRNAs are recently used as promising biomarkers for infectious diseases. The aim of this study was to identify plasma miRNAs for infection detection and monitoring the immune status of HIV infection. Methods: A cohort of 128 plasma samples from HIV-1 infected subjects and 37 samples from healthy donors were analyzed by TaqMan Low-Density Array (TLDA) method to find the differentially expressed miRNAs, in the light of HIV-1infected patients with low ( 350 cell/μL) CD4+ T cell count. Results: Seven miRNAs (miR-29a, miR-223, miR-27a, miR-19b, miR-151-3p, miR-28-5p, miR-766 and miR-30a-3p) were significantly associated with CD4+ T cell count (P Conclusions: We have identified highly sensitive and specific signatures of circulating miRNAs enabling non invasive detection and immune status monitoring of HIV-1 infection.