8 results on '"Hartmann, Sylvia"'
Search Results
2. Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells
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Goncharova, Olga, Flinner, Nadine, Bein, Julia, Döring, Claudia, Donnadieu, Emmanuel, Rikirsch, Sandy, Herling, Marco, Küppers, Ralf, Hansmann, Martin-Leo, Hartmann, Sylvia, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute of Cell Biology, IFZ, and University Hospital Essen
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[SDV]Life Sciences [q-bio] ,segmentation ,Medizin ,food and beverages ,chemokine receptors ,macromolecular substances ,cell motility ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,Article ,dissemination ,classical Hodgkin lymphoma ,anaplastic large cell lymphoma ,image analysis ,hemic and lymphatic diseases ,polycyclic compounds ,gene expression ,ddc:610 ,rosetting T cells ,ComputingMilieux_MISCELLANEOUS - Abstract
Anaplastic large cell lymphoma (ALCL) and classical Hodgkin lymphoma (cHL) are lymphomas that contain CD30-expressing tumor cells and have numerous pathological similarities. Whereas ALCL is usually diagnosed at an advanced stage, cHL more frequently presents with localized disease. The aim of the present study was to elucidate the mechanisms underlying the different clinical presentation of ALCL and cHL. Chemokine and chemokine receptor expression were similar in primary ALCL and cHL cases apart from the known overexpression of the chemokines CCL17 and CCL22 in the Hodgkin and Reed-Sternberg (HRS) cells of cHL. Consistent with the overexpression of these chemokines, primary cHL cases encountered a significantly denser T cell microenvironment than ALCL. Additionally to differences in the interaction with their microenvironment, cHL cell lines presented a lower and less efficient intrinsic cell motility than ALCL cell lines, as assessed by time-lapse microscopy in a collagen gel and transwell migration assays. We thus propose that the combination of impaired basal cell motility and differences in the interaction with the microenvironment hamper the dissemination of HRS cells in cHL when compared with the tumor cells of ALCL. CA extern
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- 2019
3. Spatial distribution of cells in Hodgkin Lymphoma
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Schäfer, Tim, Schäfer, Hendrik, Ackermann, Jörg, Dichter, Norbert, Döring, Claudia, Hartmann, Sylvia, Martin-Leo Hansmann, and Koch, Ina
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mental disorders - Abstract
Here we present our current work on the analysis of a database of HL whole slide images. We performed pre-processing and identified regions of interest (ROI) that contain CD30-positive cells in the images as described before. A CellProfiler pipeline was used to detect and measure cells in the ROI. The cells were then classified using shape descriptors and stored in a database with their 2D coordinates. This allowed for a statistical analysis of the spatial distribution of HRS cells within the tissue.
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- 2013
- Full Text
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4. Automated Image Analysis of Hodgkin Lymphoma
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Schäfer, Tim, Schäfer, Hendrik, Dichter, Norbert, Döring, Claudia, Ackermann, Jörg, Hartmann, Sylvia, Martin-Leo Hansmann, and Koch, Ina
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immune system diseases ,hemic and lymphatic diseases - Abstract
Hodgkin lymphoma is an unusual type of lymphoma, arising from malignant B-cells. Morphological and immunohistochemical featuresof malignant cells and their distribution differ from other cancer types. Based on systematic tissue image analysis, computer-aided explorationcan provide new insights into Hodgkin lymphoma pathology. Here, we report results from an image analysis of CD30 immunostained classical Hodgkin lymphoma (cHL) tissue section images. We have imple-mented an automatic procedure to handle and explore image data in Aperio's SVS format. We use pre-processing approaches to separate the imageobjects from the background, then select regions of interest and split the large images into tiles. Then, we use a CellProfiler pipeline to detect primary objects. Therefore, the images are split into their color stains using a color deconvolution approach. By setting a threshold in the CD30 stain image we identify CD30 positive cells and compute their shape descriptors. We label the cells based on size, elongation and compactness. We present results for a small set of nodular sclerosis, mixed type and non-lymphoma images.
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- 2013
- Full Text
- View/download PDF
5. DYSREGULATION OF MICRO-RNA EXPRESSION IN SPLENIC MARGINAL ZONE LYMPHOMA IN PATIENTS WITH AND WITHOUT CHRONIC HEPATITIS C VIRUS INFECTION
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Hansmann, Martin Leo, Zeuzem, Stefan, Kueppers, Ralf, Hofmann, Wolf P., Paulli, Marco, Piiper, Albrecht, Hartmann, Sylvia, Rattotti, Sara, Luca Arcaini, Lucioni, Marco, Doering, Claudia, Schmidt, Annette, Crisman, Giuliana, and Peveling-Oberhag, Jan
6. CD30 expression in neoplastic T cells of follicular T cell lymphoma is a helpful diagnostic tool in the differential diagnosis of Hodgkin lymphoma
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Stefano Pileri, Anna Portyanko, Olga Goncharova, Claudio Agostinelli, Martin-Leo Hansmann, Ralf Küppers, Elena Sabattini, Sylvia Hartmann, Jörn Meinel, Hartmann, Sylvia, Goncharova, Olga, Portyanko, Anna, Sabattini, Elena, Meinel, Jörn, Küppers, Ralf, Agostinelli, Claudio, Pileri, Stefano Aldo, and Hansmann, Martin-Leo
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Adult ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,CD30 ,T-Lymphocytes ,T cell ,Population ,Medizin ,Ki-1 Antigen ,Biology ,Stem cell marker ,DISEASE ,Pathology and Forensic Medicine ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Immunophenotyping ,stomatognathic system ,immune system diseases ,hemic and lymphatic diseases ,Biomarkers, Tumor ,medicine ,Humans ,T-cell lymphoma ,CCL17 ,REED-STERNBERG CELLS ,education ,Lymphoma, Follicular ,Aged ,education.field_of_study ,Middle Aged ,medicine.disease ,Hodgkin Disease ,Lymphoma ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female - Abstract
Follicular T cell lymphoma is derived from follicular T-helper cells. In many cases, neoplastic T cells form rosettes around Hodgkin-Reed-Sternberg-like cells, which can lead to the misdiagnosis of classical Hodgkin lymphoma. The aim of the present study was to obtain a better understanding of this rosetting phenomenon and to recognize features that are helpful in the differential diagnosis of classical Hodgkin lymphoma. Sixteen mostly elderly follicular T cell lymphoma patients (mean 66 years) were analyzed. Fifteen of the 16 follicular T cell lymphoma cases presented with Hodgkin-Reed-Sternberg-like cells, which were CD20-positive in 27% of the cases and Epstein-Barr virus-infected in nearly all cases. Frequently, the immunophenotype of rosetting neoplastic T cells differed from the bulk neoplastic cells with less numerous T-follicular helper cell markers expressed, suggesting a modulation of T-follicular helper cell marker expression in the neoplastic T cells. In 75% of the cases, variable CD30 expression was encountered in the neoplastic T cells, likely reflecting an activation state in these cells. Hodgkin-Reed-Sternberg-like cells were positive for CCL17, and follicular T cell lymphoma tumor cells expressed its receptor CCR4 at variable intensity, thus potentially explaining the phenomenon of the tumor cells' rosetting around Hodgkin-Reed-Sternberg-like cells. In summary, this study confirms the presence of Hodgkin-Reed-Sternberg-like cells in a high number of cases of follicular T cell lymphoma, suggesting that Hodgkin-Reed-Sternberg-like cells may contribute to the development of this lymphoma. Hodgkin-Reed-Sternberg-like cells in follicular T cell lymphoma cannot reliably be differentiated from the Hodgkin-Reed-Sternberg cells of classical Hodgkin lymphoma based on their immunophenotype. In contrast, demonstration of a T-follicular helper cell phenotype with CD10 and frequent CD30 expression in the neoplastic T cell population can help to establish the diagnosis of follicular T cell lymphoma, and may even indicate CD30 as a therapeutic target for these patients.
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- 2019
7. Distinctive Histogenesis and Immunological Microenvironment Based on Transcriptional Profiles of Follicular Dendritic Cell Sarcomas
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Luisa Lorenzi, Silvia Lonardi, Elena Sabattini, Stefano Pileri, Claudio Agostinelli, Maria Antonella Laginestra, Anna Gazzola, Carlo Sagramoso, Valentina Tabanelli, Federica Melle, Fabio Fuligni, Francesco Bacci, José Cabeçadas, Fabio Facchetti, Alessandro Pileri, Claudio Tripodo, Claudia Döring, Sylvia Hartmann, Maura Rossi, Anita Borges, Juan Rosai, Giovanna Motta, Ingrid Simonitsch-Klupp, Maria Rosaria Sapienza, Martin-Leo Hansmann, Elias Campo, Claudia Mannu, Laginestra, Maria Antonella, Tripodo, Claudio, Agostinelli, Claudio, Motta, Giovanna, Hartmann, Sylvia, Döring, Claudia, Rossi, Maura, Melle, Federica, Sapienza, Maria Rosaria, Tabanelli, Valentina, Pileri, Alessandro, Fuligni, Fabio, Gazzola, Anna, Mannu, Claudia, Sagramoso, Carlo Alberto, Lonardi, Silvia, Lorenzi, Luisa, Bacci, Francesco, Sabattini, Elena, Borges, Anita, Simonitsch-Klupp, Ingrid, Cabecadas, Jose, Campo, Elia, Rosai, Juan, Hansmann, Martin-Leo, Facchetti, Fabio, and Pileri, Stefano Aldo
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0301 basic medicine ,Algorithms ,B7-H1 Antigen ,Castleman Disease ,Chromatin ,Cluster Analysis ,Dendritic Cell Sarcoma, Follicular ,Gene Expression Profiling ,Gene Expression Regulation, Neoplastic ,Humans ,Programmed Cell Death 1 Ligand 2 Protein ,Programmed Cell Death 1 Receptor ,Signal Transduction ,T-Lymphocytes, Helper-Inducer ,T-Lymphocytes, Regulatory ,Up-Regulation ,Gene Regulatory Networks ,Molecular Biology ,Oncology ,Cancer Research ,Biology ,Transcriptome ,03 medical and health sciences ,medicine ,Regulation of gene expression ,Cluster Analysi ,Gene Regulatory Network ,Follicular dendritic cells ,Mesenchymal stem cell ,medicine.disease ,Algorithm ,Gene expression profiling ,030104 developmental biology ,Cancer research ,Immunohistochemistry ,Sarcoma ,Human ,Extracellular matrix organization - Abstract
Follicular dendritic cell (FDC) sarcomas are rare mesenchymal tumors with variable clinical, morphologic, and phenotypic characteristics. Transcriptome analysis was performed on multiple FDC sarcomas and compared with other mesenchymal tumors, microdissected Castleman FDCs, and normal fibroblasts. Using unsupervised analysis, FDC sarcomas clustered with microdissected FDCs, distinct from other mesenchymal tumors and fibroblasts. The specific endowment of FDC-related gene expression programs in FDC sarcomas emerged by applying a gene signature of differentially expressed genes (n = 1,289) between microdissected FDCs and fibroblasts. Supervised analysis comparing FDC sarcomas with microdissected FDCs and other mesenchymal tumors identified 370 and 2,927 differentially expressed transcripts, respectively, and on the basis of pathway enrichment analysis ascribed to signal transduction, chromatin organization, and extracellular matrix organization programs. As the transcriptome of FDC sarcomas retained similarity with FDCs, the immune landscape of FDC sarcoma was investigated by applying the CIBERSORT algorithm to FDC sarcomas and non-FDC mesenchymal tumors and demonstrated that FDC sarcomas were enriched in T follicular helper (TFH) and T regulatory (TREG) cell populations, as confirmed in situ by immunohistochemistry. The enrichment in specific T-cell subsets prompted investigating the mRNA expression of the inhibitory immune receptor PD-1 and its ligands PD-L1 and PD-L2, which were found to be significantly upregulated in FDC sarcomas as compared with other mesenchymal tumors, a finding also confirmed in situ. Here, it is demonstrated for the first time the transcriptional relationship of FDC sarcomas with nonmalignant FDCs and their distinction from other mesenchymal tumors. Implications: The current study provides evidence of a peculiar immune microenvironment associated with FDC sarcomas that may have clinical utility. Mol Cancer Res; 15(5); 541–52. ©2017 AACR.
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- 2017
8. miRNA expression profiling divides follicular dendritic cell sarcomas into two groups, related to fibroblasts and myopericytomas or Castleman's disease
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Luisa Lorenzi, Claudio Agostinelli, Soo Jeong Portscher-Kim, Martin-Leo Hansmann, Holger Hackstein, Claudia Döring, Silvia Lonardi, Jay Mehta, Sylvia Hartmann, José Cabeçadas, Stefano Pileri, Fabio Facchetti, Stefan Kippenberger, Daniel Martinez, Ingrid Simonitsch-Klupp, Elias Campo, Anita Borges, Fabio Fuligni, Pier Paolo Piccaluga, Hartmann, Sylvia, Döring, Claudia, Agostinelli, Claudio, Portscher-Kim, Soo-Jeong, Lonardi, Silvia, Lorenzi, Luisa, Fuligni, Fabio, Martinez, Daniel, Mehta, Jay, Borges, Anita, Hackstein, Holger, Kippenberger, Stefan, Piccaluga, Pier Paolo, Simonitsch-Klupp, Ingrid, Cabeçadas, José, Campo, Elia, Facchetti, Fabio, Pileri, Stefano A., and Hansmann, Martin-Leo
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Cancer Research ,Adolescent ,Cell of origin ,BRAF ,Follicular dendritic cell sarcoma ,Follicular dendritic cells ,miRNA profiling ,Podoplanin ,Oncology ,Dendritic Cell Sarcoma, Follicular ,Biology ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,microRNA ,medicine ,Biomarkers, Tumor ,Humans ,Aged ,Aged, 80 and over ,Castleman Disease ,Gene Expression Profiling ,Mesenchymal stem cell ,Fibroblasts ,Middle Aged ,medicine.disease ,Microarray Analysis ,Immunohistochemistry ,Gene expression profiling ,MicroRNAs ,030220 oncology & carcinogenesis ,Female ,Sarcoma ,Follicular dendritic cell ,Dendritic Cells, Follicular ,030215 immunology - Abstract
Follicular dendritic cell (FDC) sarcomas are rare mesenchymal tumours, which are fatal in 20% of the patients and usually occur in secondary lymphoid organs or extranodal localizations. Due to the rareness of these tumours, only few studies have been conducted on molecular level. In the present study, we performed microRNA (miRNA) profiling of 31 FDC sarcomas and identified two subgroups, one with high miRNA expression and the other group with low miRNA expression levels. The first group showed a strong similarity to fibroblasts and myopericytomas, whereas the second group was more closely related to FDCs from Castleman's disease. Both groups showed important differences compared with myeloid-derived dendritic cells, confirming mesenchymal origin of FDCs and their derived sarcomas. The two FDC sarcoma groups did not differ on morphological grounds, mitotic activity or BRAF mutation status. However, patients of group I presented a tendency to a shorter overall survival and more frequent podoplanin expression by immunohistochemistry. The importance of these newly recognized FDC sarcoma subgroups in terms of clinical behaviour and therapeutic implications should be assessed in a larger cohort in future studies.
- Published
- 2016
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