1. Spontaneous liver disease in wild-type C57BL/6JOlaHsd mice fed semisynthetic diet
- Author
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Ronda, Onne A H O, van de Heijning, Bert J M, de Bruin, Alain, Thomas, Rachel E, Martini, Ingrid, Koehorst, Martijn, Gerding, Albert, Koster, Mirjam H, Bloks, Vincent W, Jurdzinski, Angelika, Mulder, Niels L, Havinga, Rick, van der Beek, Eline M, Reijngoud, Dirk-Jan, Kuipers, Folkert, Verkade, Henkjan J, Sub General Pharmacology, Dep Biomolecular Health Sciences, Pathobiologie, dPB RMSC, LS Pathobiologie, Sub General Pharmacology, Dep Biomolecular Health Sciences, Pathobiologie, dPB RMSC, and LS Pathobiologie
- Subjects
0301 basic medicine ,Male ,Very low-density lipoprotein ,Lipoproteins, VLDL/blood ,Physiology ,Lipoproteins, VLDL ,Inbred C57BL ,Biochemistry ,Mice ,Liver disease ,0302 clinical medicine ,ABSORPTION ,Medicine and Health Sciences ,Bile ,Immune Response ,VLDL/blood ,Multidisciplinary ,Bile acid ,Chemistry ,NONALCOHOLIC STEATOHEPATITIS ,Animal Feed/adverse effects ,CHOLESTEROL ,Liver Diseases ,Fatty liver ,Fatty Acids ,Lipids ,Body Fluids ,Multidisciplinary Sciences ,DEFICIENCY ,Triglycerides/blood ,Physiological Parameters ,Liver ,Science & Technology - Other Topics ,Medicine ,030211 gastroenterology & hepatology ,Female ,Fatty Acids/blood ,FATTY-ACIDS ,Anatomy ,METABOLIC ADAPTATION ,Research Article ,Liver/metabolism ,EXPRESSION ,medicine.medical_specialty ,Histology ,medicine.drug_class ,Lipoproteins ,Science ,Immunology ,Gastroenterology and Hepatology ,Biology ,Liver weight ,Bile Acids and Salts ,03 medical and health sciences ,HEPATITIS ,Signs and Symptoms ,Sex Factors ,Internal medicine ,medicine ,Weaning ,Animals ,Humans ,Secretion ,Triglycerides ,Nutrition ,Inflammation ,Science & Technology ,Animal ,TRANSPORTER ,Body Weight ,Wild type ,Biology and Life Sciences ,Lipid metabolism ,Metabolism ,Fatty Liver/blood ,Lipid Metabolism ,medicine.disease ,Animal Feed ,Diet ,Fatty Liver ,Mice, Inbred C57BL ,Disease Models, Animal ,Bile Acids and Salts/blood ,030104 developmental biology ,Endocrinology ,Disease Models ,Steatosis ,Clinical Medicine ,Physiological Processes ,BILE-ACID ,Lipoprotein - Abstract
Mouse models are frequently used to study mechanisms of human diseases. Recently, we observed a spontaneous bimodal variation in liver weight in C57BL/6JOlaHsd mice fed a semisynthetic diet. We now characterized the spontaneous variation in liver weight and its relationship with parameters of hepatic lipid and bile acid (BA) metabolism. In male C57BL/6JOlaHsd mice fed AIN-93G from birth to postnatal day (PN)70, we measured plasma BA, lipids, Very low-density lipoprotein (VLDL)-triglyceride (TG) secretion, and hepatic mRNA expression patterns. Mice were sacrificed at PN21, PN42, PN63 and PN70. Liver weight distribution was bimodal at PN70. Mice could be subdivided into two nonoverlapping groups based on liver weight: 0.6 SD 0.1 g (approximately one-third of mice, small liver; SL), and 1.0 SD 0.1 g (normal liver; NL; pversusthe NL group. Plasma BA concentration was 14-fold higher in SL (p
- Published
- 2020