49 results on '"Hezhen Wu"'
Search Results
2. Tomography with sparseness regularisation for ultrasonic velocity imaging
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Wei Zhu, Shangxu Wang, Xu Chang, Hongyu Zhai, Taiming He, and Hezhen Wu
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Geophysics ,Geology ,Management, Monitoring, Policy and Law ,Industrial and Manufacturing Engineering - Abstract
Ultrasonic tomography, which is widely used in the study of the fracturing process of rocks, often exhibits low resolution due to insufficient ray coverage, particularly while evaluating the three-dimensional (3D) fractures. To resolve this issue, we adopted sparseness regularisation in tomography to reconstruct the ultrasonic velocity of rocks. Both numerical and laboratory experiments demonstrate that tomography with sparseness regularisation generates velocity images with clear fracture morphology than that with Tikhonov regularisation. Dynamic monitoring of the fracturing process of a granite slab with two-dimensional (2D) velocity images can reveal the accurate development of the fracturing process. The experiment on the internal structure of tight sandstone after hydraulic fracturing reveals demarcated low-velocity regions in the 3D ultrasonic velocity images of tomography with sparseness regularisation. These low-velocity regions correspond to the positions of the fractures when compared to the X-ray scanning images. Thus, tomography with sparseness regularisation can improve the resolution of ultrasonic velocity images, which can be used to accurately describe the fracture development and strain localisation during rock deformation.
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- 2022
3. Glycolysis, a new mechanism of oleuropein against liver tumor
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Zongchao Hong, Yi Lu, Bo Liu, Chongwang Ran, Xia Lei, Mengfan Wang, Songtao Wu, Yanfang Yang, and Hezhen Wu
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Pharmacology ,Complementary and alternative medicine ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine - Published
- 2023
4. Extract from Rostellularia procumbens (L.) Nees Inhibits Thrombosis and Platelet Aggregation by Regulating Integrin β3 and MAPK Pathways
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Ying Zhang, Xingpan Wu, Hezhen Wu, Yanfang Yang, Zixin Yuan, Tianshun Wang, Zhongzhu Ai, Zongchao Hong, and Bo Liu
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MAPK/ERK pathway ,biology ,medicine.diagnostic_test ,Chemistry ,General Chemical Engineering ,Integrin ,General Chemistry ,Pharmacology ,Article ,eye diseases ,In vitro ,Western blot ,In vivo ,Antithrombotic ,biology.protein ,medicine ,Phosphorylation ,Platelet ,sense organs ,QD1-999 - Abstract
Aim of study The main objective of this study was to investigate the antithrombotic and antiplatelet effect of the extract from Rostellularia procumbenss (L.) Nees and understand the mechanisms by which it exerts its antithrombotic and antiplatelet mechanisms. Materials and methods The antithrombotic effective parts (RPE) were isolated using D101 macroporous adsorption resin and potential active ingredients (JAC) were isolated using the preparative liquid-phase method. The lactate dehydrogenase kit was used to determine the toxicity of RPE and JAC to platelets. The antiadhesion effect of RPE and JAC on platelets was observed by fluorescence microscopy with rhodamine phalloidin. Antithrombotic efficacy of RPE and JAC in vivo was evaluated by establishing a rat tail thrombosis model. Contents of p-selectin, TXB2, and 6-keto-PGF1α in rat serum were measured using an enzyme-linked immunosorbent (ELISA) assay, and the rat black tail rate was measured to prove the protective effect of RPE and JAC on the tail thrombus rat model. Western blot was used for detection of serum-related proteins in the tail thrombus rat model. Results The results showed that RPE had antithrombotic and antiplatelet effects. RPE and JAC have no toxicity to platelets. In vitro experiments showed that RPE and JAC had antiadhesion effects on platelets. In vivo experiments showed that RPE significantly inhibited the increase of p-selectin and TXB2 and significantly increased the content of 6-keto-PGF1α in the serum of rats. Western blot results demonstrated that RPE and JDB significantly inhibited the phosphorylation of the MAPK protein family in the platelets of rats, and RPE also significantly inhibited the phosphorylation of β3 protein. Conclusions RPE has antithrombotic and antiplatelet activity in vivo and vitro. Its mechanism may be via preventing integrin αIIbβ3 activation, which in turn leads to the inhibition of the phosphorylation of the MAPK family and further suppresses TXA2, which leads to the antithrombotic and antiplatelet effects.
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- 2020
5. Bioactive ingredients obtained from Cortex Fraxini impair interactions between FAS and GPI
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Yanfang Yang, Hezhen Wu, Li Tong, Bo Liu, Zhongzhu Ai, Song-Tao Wu, Pengtao You, and Zongchao Hong
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0301 basic medicine ,Aesculus ,Proteomics ,Biochemistry ,Transcriptome ,Cell membrane ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Physiology (medical) ,medicine ,biology ,Microscale thermophoresis ,Fatty Acids ,Glucose-6-Phosphate Isomerase ,Lipid metabolism ,Fatty acid synthase ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,biology.protein ,Target protein ,Fatty Acid Synthases ,Scopolin ,030217 neurology & neurosurgery ,Drugs, Chinese Herbal - Abstract
The high expression of fatty acid synthase (FAS) in tumor cells is consistent with their elevated requirement for fatty acids for cell membrane synthesis and energy supply to support their almost unlimited proliferation. The expression levels of FAS in tumor cells are related to their proliferation, invasion, and metastasis. This study investigated the possible bioactive ingredients (fraxin, esculetin, scopolin et al.) of Cortex Fraxini and their effects on the interaction between specific proteins. We used microscale thermophoresis (MST) to show that our target protein, FAS (screened by combining transcriptome and network pharmacology), bound to the active compounds in Cortex Fraxini. It was found that FAS bound strongly to Glucose-6-phosphate isomerase (GPI), and that scopolin could affect this interaction by proteomics and MST. The results of this study demonstrate that the active compounds in Cortex Fraxini could play an anti-tumor role by binding to FAS and inhibiting the interactions between FAS and GPI to affect glucose and lipid metabolism, and that the protein pathway is a potential novel target for tumor treatment.
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- 2020
6. Reveals of candidate active ingredients in Justicia and its anti-thrombotic action of mechanism based on network pharmacology approach and experimental validation
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Ting Zhang, Ying Zhang, Hezhen Wu, Lu Yi, Yunfeng Yao, Yanfang Yang, Zongchao Hong, Bo Liu, and Zhou-Tao Xie
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Blood Platelets ,Male ,rac1 GTP-Binding Protein ,ATP Binding Cassette Transporter, Subfamily B ,Molecular biology ,Computer science ,Science ,Mechanism based ,Drug development ,Computational biology ,Lignans ,Article ,Mice ,Fibrinolytic Agents ,Interaction network ,Justicia ,Network pharmacology ,Animals ,Humans ,Gene Regulatory Networks ,Protein Interaction Maps ,KEGG ,Inhibitory effect ,Cells, Cultured ,Cyclic Nucleotide Phosphodiesterases, Type 5 ,Active ingredient ,Mice, Inbred BALB C ,Multidisciplinary ,Drug discovery ,Mechanism (biology) ,Dioxolanes ,Experimental validation ,Proto-Oncogene Proteins c-met ,Chemokine CXCL12 ,Computational biology and bioinformatics ,Matrix Metalloproteinase 9 ,Medicine - Abstract
Thrombotic diseases seriously threaten human life. Justicia, as a common Chinese medicine, is usually used for anti-inflammatory treatment, and further studies have found that it has an inhibitory effect on platelet aggregation. Therefore, it can be inferred that Justicia can be used as a therapeutic drug for thrombosis. This work aims to reveal the pharmacological mechanism of the anti-thrombotic effect of Justicia through network pharmacology combined with wet experimental verification. During the analysis, 461 compound targets were predicted from various databases and 881 thrombus-related targets were collected. Then, herb-compound-target network and protein–protein interaction network of disease and prediction targets were constructed and cluster analysis was applied to further explore the connection between the targets. In addition, Gene Ontology (GO) and pathway (KEGG) enrichment were used to further determine the association between target proteins and diseases. Finally, the expression of hub target proteins of the core component and the anti-thrombotic effect of Justicia’s core compounds were verified by experiments. In conclusion, the core bioactive components, especially justicidin D, can reduce thrombosis by regulating F2, MMP9, CXCL12, MET, RAC1, PDE5A, and ABCB1. The combination of network pharmacology and the experimental research strategies proposed in this paper provides a comprehensive method for systematically exploring the therapeutic mechanism of multi-component medicine.
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- 2021
7. Erratum for Ferroptosis-related Genes for Overall Survival Prediction in Patients with Colorectal Cancer can be Inhibited by Gallic acid
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Zongchao Hong, Peili Tang, Bo Liu, Chongwang Ran, Chong Yuan, Ying Zhang, Yi Lu, Xueyun Duan, Yanfang Yang, and Hezhen Wu
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Cell Biology ,Molecular Biology ,Applied Microbiology and Biotechnology ,Ecology, Evolution, Behavior and Systematics ,Developmental Biology - Published
- 2022
8. Inhibition effect of oxyepiberberine isolated from Coptis chinensis franch. On non-small cell lung cancer based on a network pharmacology approach and experimental validation
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Xingpan Wu, Zongchao Hong, Yunfeng Yao, Tianshun Wang, Yanfang Yang, Fu Yingjie, Zixin Yuan, Ying Zhang, and Hezhen Wu
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Coptis chinensis ,Lung Neoplasms ,Clone (cell biology) ,Apoptosis ,MMP9 ,Network Pharmacology ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Drug Discovery ,Humans ,MTT assay ,030304 developmental biology ,Cell Proliferation ,Pharmacology ,0303 health sciences ,biology ,Chemistry ,Cell growth ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Gene Expression Regulation, Neoplastic ,Molecular Docking Simulation ,Docking (molecular) ,A549 Cells ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research - Abstract
Ethnopharmacological relevance As an important Chinese herb, Coptis chinensis Franch. (Huanglian, HL) has a long history of usage for clearing heat, eliminating dampness, purging fire and detoxification in Traditional Chinese Medicine (TCM). HL, also called goldthread, was frequently used for the treatment of typhoid, tuberculosis, epidemic cerebrospinal meningitis, pertussis, and other lung-related diseases. Modern research has shown that HL and its main compounds also have anti-tumor effects. However, studies have not reported whether its main compounds inhibit Non-small cell lung cancer (NSCLC) development and progression. Objective This study aimed to find out the potential targets and mechanisms of Oxyepiberberine (OPB) isolated from HL in the treatment of NSCLC, using network pharmacology and biological experimental. Methods Silica gel chromatography column was used to isolate OPB from HL, and the structure of OPB was elucidated using different spectroscopic analysis methods, including 1H-nuclear magnetic resonance (NMR), 13C-NMR and electrospray ionization mass spectrometry (ESI/MS). MTT assay was performed to determine cell proliferation of OPB on A549, H1975 and BEAS-2B cells. Then, the potential targets, pathways and hub genes of OPB for treating NSCLC were screened out through network pharmacology. Based on the results of network pharmacology, core targets of OPB for treating NSCLC were docking with OPB via molecular docking. Wound healing, plate clone, Hoechst staining, and western blot assay were used to verify the function of OPB in treatment of NSCLC. Results OPB was isolated from the HL, its molecular formula was identified as C20H17NO5. Through MTT, OPB significantly inhibited the proliferation of H1975 cells and A549 cells, and A549 was chosen as the test cancer cell. Through network pharmacology, 22 potential targets, 156 related-pathways, and 6 hub genes were screened out. The results of molecular docking showed that SRC, BRAF, and MMP9 were the core targets of OPB against NSCLC. Through biological experimental, it was found that OPB inhibited growth and migration of A549 cells. In addition, OPB induced apoptosis in A549 cells. Through western blot assay, the expressions of Src, ERK1/2 and other four proteins were down-regulated, which suggested that OPB inhibited the proliferation of lung cancer cells by down-regulating SRC-FAK-RAS-RAF-MEK-ERK pathway, so as to achieve the anti-NSCLC effect. Conclusion Our study demonstrated that anti-NSCLC effect of OPB through network and experiments, which provided a theoretical basis for the clinical antitumor of OPB, and provided a foundation for further study of OPB.
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- 2021
9. A New Neolignan from Justicia Procumbens
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Xiong Weichen, Yanfang Yang, Bo Liu, Xiong Yiyi, Zhou-Tao Xie, and Hezhen Wu
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Lariciresinol ,biology ,010405 organic chemistry ,Stereochemistry ,Absolute configuration ,Plant Science ,General Chemistry ,biology.organism_classification ,01 natural sciences ,Justicia procumbens ,General Biochemistry, Genetics and Molecular Biology ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Secoisolariciresinol - Abstract
A new neolignan, named (7′S,8′R)-5,3′-dimethoxy-4,4′,9′-trihydroxy-2-8′,9-O-7′-neolignan (1), along with two known compounds, secoisolariciresinol (2) and lariciresinol (3), was isolated from the whole plant of Justicia procumbens L. The structure of 1 was established by spectroscopic methods, and the absolute configuration was determined using circular dichroic (CD) spectra.
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- 2020
10. 14-Deoxycoleon U-induced endoplasmic reticulum stress-mediated apoptosis, autophagy, and cell cycle arrest in lung adenocarcinoma
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Chaozhi Ma, Hezhen Wu, Yanfang Yang, Pengtao You, Xiao-Zhi Peng, Yijun Tu, Yu Xia, Yanwen Liu, and San Fu
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0301 basic medicine ,A549 cell ,Cyclin-dependent kinase 1 ,Cell cycle checkpoint ,Cell growth ,Chemistry ,Autophagy ,Cell cycle ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,Pharmacology (medical) ,Viability assay - Abstract
Objective 14-Deoxycoleon U is a natural abietane-type diterpene and exerts an inhibitory effect on tumor cells proliferation, which suggests that 14-Deoxycoleon U may be a potent anti-cancerous lead compound for lung cancer treatment. This study was to evaluate potential of 14-Deoxycoleon U to treat lung adenocarcinoma in vitro and in vivo. Methods In the present study, the cell viability and apoptosis morphology of 14-Deoxycoleon U-treated A549 and LLC cells were explored using cell counting kit-8 assay and Hoechst 33258 staining. Then, the protein expressions about apoptosis, endoplasmic reticulum (ER) stress, autophagy and cell cycle were measured using Western blot. The autophagosome formation of 14-Deoxycoleon U-treated A549 cells was visualized using a confocal microscopy. LLC lung adenocarcinoma model was established. Results The results indicated that 14-Deoxycoleon U significantly inhibited A549 and LLC cell proliferation in a dose-dependent manner via caspase-dependent apoptosis. Furthermore, apoptosis of both cells was mediated by 14-Deoxycoleon U-induced ER stress. In addition, 14-Deoxycoleon U-induced A549 and LLC cell autophagy, thus promoting their death. Moreover, 14-Deoxycoleon U-induced cell cycle arrest in both cells via inhibition of cyclin D3, cyclin-dependent kinase 6, CDC2 and up-regulation of p21. In vivo results showed that administration of 14-Deoxycoleon U significantly suppressed LLC growth and adverse effects of 14-Deoxycoleon U on organs might be lower than of adriamycin. Conclusion Overall, our results demonstrated that 14-Deoxycoleon U represses in vitro and in vivo growth of lung adenocarcinoma through ER stress-mediated apoptosis accompanied by autophagy and cell cycle arrest.
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- 2019
11. Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity
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Bo Yu, Yijun Tu, Ling Zhai, Pengtao You, Yanfang Yang, Hezhen Wu, Dan Liu, Yu Xia, Yanwen Liu, Chaozhi Ma, and Hanxiong Dan
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Male ,0301 basic medicine ,MMP2 ,Smad Proteins ,CCL4 ,SMAD ,MMP9 ,Liver Cirrhosis, Experimental ,Rats, Sprague-Dawley ,Transforming Growth Factor beta1 ,Interferon-gamma ,03 medical and health sciences ,Downregulation and upregulation ,Fibrosis ,medicine ,Animals ,Phosphorylation ,Carbon Tetrachloride ,TIMP1 ,Pharmacology ,Extracellular Matrix Proteins ,Dose-Response Relationship, Drug ,Chemistry ,Interleukin-17 ,General Medicine ,medicine.disease ,Extracellular Matrix ,030104 developmental biology ,Liver ,Cytoprotection ,Proteolysis ,Cancer research ,Interleukin-4 ,Chemical and Drug Induced Liver Injury ,Inflammation Mediators ,Hepatic fibrosis ,Drugs, Chinese Herbal ,Signal Transduction - Abstract
Yu Gan Long (YGL) is a Chinese traditional herbal medicine that has been used in the treatment of liver fibrosis for many years in clinical practice. However, its anti-hepatofibrotic mechanism has not been studied yet. In this study, the effect and mechanism of YGL in reducing liver fibrosis was demonstrated in vivo. Our results showed that liver fibrosis biomarkers collagen IV (Col IV), type III precollagen (PCIII), hyaluronuc acid (HA) and laminin (LN), were increased after CCl4 treatment and decreased by YGL. Among the liver fibrosis indicators, α-smooth muscle actin (α-SMA) was decreased by YGL in the CCl4-treated rats, while MMP2 and MMP9 was upregulated followed by TIMP1 downregulation. Proteins involved in liver fibrosis such as p-Smad2, p-Smad3 and Smad4 were down-regulated, while Smad7 protein was up-regulated by YGL after CCl4-induced liver damage. YGL also suppressed the increase of TGF-β1, TNF-α, IL-1β, IL-6, IL-4 and IL-17 A induced by CCl4 treatment, while promoted IFN-γ expression. Finally, the transcription factors ROR-γt and GATA3 were decreased, while T-bet was increased after YGL treatment. These results suggested that YGL attenuated CCl4-induced hepatic fibrosis by accelerating the extracellular matrix degradation, blocking the TGF-β1/Smad signaling pathway and modulating the balance among IL-4, IL-17 A and IFN-γ, demonstrating YGL protective effect and its potential mechanisms in treating liver fibrosis.
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- 2018
12. Table S1 - Supplemental material for Investigation of Anti-SARS, MERS, and COVID-19 Effect of Jinhua Qinggan Granules Based on a Network Pharmacology and Molecular Docking Approach
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Zhang, Ying, Yunfeng Yao, Yanfang Yang, and Hezhen Wu
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FOS: Clinical medicine ,111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified - Abstract
Supplemental material, Table S1, for Investigation of Anti-SARS, MERS, and COVID-19 Effect of Jinhua Qinggan Granules Based on a Network Pharmacology and Molecular Docking Approach by Ying Zhang, Yunfeng Yao, Yanfang Yang and Hezhen Wu in Natural Product Communications
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- 2021
- Full Text
- View/download PDF
13. Supplementary Material 1 - Supplemental material for Network Pharmacology Integrated Molecular Docking Analysis of Potential Common Mechanisms of Shu-Feng-Jie-Du Capsule in the Treatment of SARS, MERS, and COVID-19
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Zhang, Ying, Xie, Yi, Yu, Bing, Yuan, Chong, Zixin Yuan, Zongchao Hong, Hezhen Wu, and Yanfang Yang
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FOS: Clinical medicine ,111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified - Abstract
Supplemental material, Supplementary Material 1, for Network Pharmacology Integrated Molecular Docking Analysis of Potential Common Mechanisms of Shu-Feng-Jie-Du Capsule in the Treatment of SARS, MERS, and COVID-19 by Ying Zhang, Yi Xie, Bing Yu, Chong Yuan, Zixin Yuan, Zongchao Hong, Hezhen Wu and Yanfang Yang in Natural Product Communications
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- 2020
- Full Text
- View/download PDF
14. Hierarchical Nanostructuring Array Enhances Mid-Hybridization for Accurate Herbal Identification via ITS2 DNA Barcode
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Ruixue Shi, Lei Xu, Bisheng Huang, Li Liu, Guo-Jun Zhang, Hao Lu, Zhigang Hu, Hezhen Wu, Yanju Liu, Shilin Chen, and Fan Yang
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Plants, Medicinal ,DNA, Plant ,Chemistry ,DNA–DNA hybridization ,010401 analytical chemistry ,Nucleic Acid Hybridization ,Biointerface ,Nanotechnology ,Electrochemical Techniques ,Amplicon ,010402 general chemistry ,Barcode ,01 natural sciences ,DNA barcoding ,0104 chemical sciences ,Analytical Chemistry ,law.invention ,Nanostructures ,Nucleic acid thermodynamics ,chemistry.chemical_compound ,law ,DNA Barcoding, Taxonomic ,Identification (biology) ,DNA ,Drugs, Chinese Herbal - Abstract
It remains a technical challenge to accurately identify close species of herbal medicines, especially from adulterants, because of their highly identical phenotypes and chemical compositions. Here, we report a direct, sequencing-free, high-curvature nanostructuring-based electrochemical herb sensor (nanoE-herb sensor) to identify herbal species quickly and accurately using ITS2 barcodes. We engineer a nano-roughened carbon-supported gold nanostructuring array by photolithograph-free, one-step electrodeposition. The 3D fractal nanostructures exhibit a high deflection angle that largely enhances DNA hybridization efficiency, particularly for the midcomplementary hybridization, as compared to the 2D planar surface. More importantly, such a trans-scale array biointerface (including macroscale carbon and nanoscale gold branches) can overcome the detection barrier of slow diffusion of a long genomic sequence and inaccessibility of the sequestered variations in ITS2 secondary structures through the out-protruded 3D functional nanostructures. Our nanoE-herb sensor achieves a detection limit of 0.18 fM for the 64-mer fragment of saffron ITS2 barcode with midhybridization and shows superior specificity against even single-base mismatch. The sensor also precisely differentiates saffron from six other adulterants by directly detecting unpurified asymmetric PCR amplicons (∼500 bp) with ITS2 sequences, suggesting its great potential in the field identification of herbal medicinal species and pathogenic bacteria with specific DNA barcodes.
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- 2019
15. Scutellarin suppresses neuroinflammation via the inhibition of the AKT/NF-κB and p38/JNK pathway in LPS-induced BV-2 microglial cells
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Xin Chen, Jun Wang, San Fu, Chaozhi Ma, Hezhen Wu, Yanfang Yang, Yu Xia, Yanwen Liu, Pengtao You, Kun Yu, Dan Liu, and Xiaochuan Ye
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Lipopolysaccharides ,0301 basic medicine ,MAP Kinase Signaling System ,p38 mitogen-activated protein kinases ,Anti-Inflammatory Agents ,Glucuronates ,Cell Line ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Apigenin ,Protein kinase B ,Neuroinflammation ,PI3K/AKT/mTOR pathway ,Pharmacology ,Scutellarin ,Microglia ,NF-kappa B ,NF-κB ,General Medicine ,Cell biology ,Neuroprotective Agents ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Phosphorylation ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery - Abstract
In vitro and in vivo studies indicate that scutellarin (SCU) exerts anti-inflammatory effects in the central nervous system (CNS) and inhibits microglia activation. This study investigated the anti-neuroinflammation molecular mechanisms exerted by scutellarin in LPS-induced BV-2 cells. The results showed that production of TNF-α, IL-1β, IL-6, and NO and TNF-α, IL-1β, IL-6, and iNOS mRNA were inhibited by scutellarin, which was independent of cytotoxicity as assessed by a CCK8 assay. Western blot analysis indicated that NF-κB-p65 phosphorylation was suppressed by scutellarin via inhibition of IκB degradation and IKKβ activation, which coincided with blockage of nuclear translocation of NF-κB as shown by immunofluorescent staining. Consistent with the inhibition of NF-κB, scutellarin inhibited the phosphorylation of p38, JNK, and AKT without affecting phosphorylation of ERK1/2 or PI3K in LPS-induced BV-2 cells. Overall, the present study suggests that scutellarin inhibits the production of pro-inflammatory mediators via inhibition of the IKK-dependent NF-κB and p38/JNK signaling pathway, which inhibits microglia activation and exerts anti-inflammation, indicating its potential therapeutic effect for neurodegenerative and cerebrovascular diseases.
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- 2018
16. The bioactive ingredients in Actinidia chinensis Planch. Inhibit liver cancer by inducing apoptosis
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Chongwang Ran, Peili Tang, Lu Yi, Xueyun Duan, Ju Huang, Hezhen Wu, Yanfang Yang, and Zongchao Hong
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Cell Survival ,Actinidia ,Apoptosis ,Caspase 3 ,Biology ,Pharmacology ,Caspase 8 ,chemistry.chemical_compound ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Gene Regulatory Networks ,Caspase-9 ,Plant Extracts ,Liver Neoplasms ,Cancer ,medicine.disease ,Antineoplastic Agents, Phytogenic ,Gene Expression Regulation, Neoplastic ,chemistry ,biology.protein ,Astragalin ,Signal transduction ,Liver cancer ,Drugs, Chinese Herbal ,Phytotherapy - Abstract
Ethnopharmacological relevance Actinidia chinensis Planch. (ACP) is a common traditional Chinese medicine, which is mostly used for cancer treatment clinically. Liver cancer is a refractory tumor with a high incidence. Although ACP has been reported in the treatment of liver cancer, its possible mechanism of action is little known. Aim of study The aim of this paper was to investigate the active components of ACP in the treatment of liver cancer and the related mechanisms by a network pharmacology approach. Methods The active components of ACP and the corresponding targets were obtained from multiple databases. Cytoscape software and STRING database were used to build the "herb-component-target (H-C-T)" network and protein–protein interactions (PPI) network. The key components and targets were further predicted by the Cytohubba plug-in in Cytoscape. Then, experiments were carried out on HepG2 cell line and Huh7 cell line to verify the effects and related mechanisms of the key compounds in ACP. Results 28 active components in ACP and 1299 related targets were screened out according to two indicators, oral bioavailability (OB) and drug-likeness (DL). The key compounds predicted include rutinum, astragalin, and L-epicatechin, and the main signaling pathways focus on apoptosis. Astragalin, a key compound in ACP, could inhibit the expression of Bcl-2, up-regulate the expression of Bax, cleaved caspase 3, cleaved caspase 8, and cleaved caspase 9, and regulate the apoptosis signaling pathway to inhibit the proliferation of liver cancer cells to play a therapeutic role in anti-liver cancer. Conclusions These results suggest that ACP can alleviate the progression of liver cancer through the mechanisms predicted by network pharmacology, and provide a basis for the further understanding of the application of ACP in anti-cancer.
- Published
- 2021
17. Revealing the mechanism of 'Huai Hua San' in the treatment of ulcerative colitis based on network pharmacology and experimental study
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Peng-yu Chen, Bing Yu, Zongchao Hong, Yanfang Yang, Chen Wang, Chong Yuan, Xue-cheng Xiao, Hezhen Wu, Ying Zhang, and Xin-ge Ke
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Male ,Cell Survival ,Network Pharmacology ,Pharmacology ,Proinflammatory cytokine ,Mice ,Random Allocation ,03 medical and health sciences ,0302 clinical medicine ,Drug Discovery ,VEGF Signaling Pathway ,Animals ,Medicine ,Colitis ,KEGG ,Mesalamine ,Protein kinase B ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,Mice, Inbred BALB C ,0303 health sciences ,Dose-Response Relationship, Drug ,business.industry ,Akt/PKB signaling pathway ,Anti-Inflammatory Agents, Non-Steroidal ,medicine.disease ,Molecular Docking Simulation ,RAW 264.7 Cells ,Mechanism of action ,030220 oncology & carcinogenesis ,Colitis, Ulcerative ,medicine.symptom ,business ,Drugs, Chinese Herbal ,Phytotherapy - Abstract
Ethnopharmacological relevance “Huai Hua San” (HHS) is one of the first hundred ancient classic prescriptions drugs, which is commonly used to treat hemorrhoids, colitis, and other symptoms of wind heat in stool. However, the potential molecular mechanism of action of this substance remains unclear. Aims of the study: In this study, we explored the active compounds of HHS for the treatment of ulcerative colitis (UC), predicted the potential targets of the drug, and studied its mechanism of action through network pharmacology via in vitro and in vivo experiments. Materials and methods First, we identified the active compounds and key targets of HHS for treating UC via network pharmacology. The key signaling pathways associated with the anti-inflammatory effect of HHS were analyzed. The anti-inflammatory effects of HHS and its active compounds were studied using the RAW264.7 inflammatory cell model in vitro. Furthermore, we used the dextran sulfate sodium (DSS) mouse model to explore the efficacy and mechanism of HHS in UC in vivo, and the expression level of key proteins were detected by Western blotting. Results In all, 23 compounds and 97 targets were obtained from TCMSP database, PharmMapper database, and GeneCards database. After enrichment via Kyoto Encyclopedia of Genes and Genomes (KEGG), HIF-1 signaling pathway, PI3K/AKT signaling pathway, and VEGF signaling pathway were identified to be the top three signaling pathways associated with UC treatment. The results of molecular docking showed that the docking scores of the top 10 active compounds were higher than the threshold values. In vitro, different concentrations of HHS and the four main active compounds could effectively inhibit the release of inflammatory cytokines interleukin (IL)-6, tumor necrosis factor (TNF)–α, and IL-1 β. In vivo, HHS could alleviate UC symptoms. Conclusion Taken together, the treatment of UC with HHS may alleviate the inflammatory response of the colon, and HHS mainly inhibits the EGFR/PI3K/AKT/HIF-1/VEGF signaling pathways.
- Published
- 2021
18. Three-Dimensional Ultrasonic Imaging and Acoustic Emission Monitoring of Hydraulic Fractures in Tight Sandstone
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Wei Zhu, Xu Chang, Hezhen Wu, Hongyu Zhai, and Shangxu Wang
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Dilatant ,Technology ,sparse tomography ,QH301-705.5 ,tight sandstone ,QC1-999 ,hydraulic fracturing ,Hydraulic fracturing ,Rock mechanics ,Fluid dynamics ,General Materials Science ,Biology (General) ,Petrology ,QD1-999 ,Instrumentation ,Fluid Flow and Transfer Processes ,Physics ,Process Chemistry and Technology ,Tight oil ,General Engineering ,Engineering (General). Civil engineering (General) ,Computer Science Applications ,Chemistry ,Acoustic emission ,Fracture (geology) ,TA1-2040 ,acoustic emission ,Differential stress ,Geology - Abstract
Hydraulic fracturing is an important means for the development of tight oil and gas reservoirs. Laboratory rock mechanics experiments can be used to better understand the mechanism of hydraulic fracture. Therefore, in this study we carried out hydraulic fracturing experiments on Triassic Yanchang Formation tight sandstone from the Ordos Basin, China. Sparse tomography was used to obtain ultrasonic velocity images of the sample during hydraulic fracturing. Then, combining the changes in rock mechanics parameters, acoustic emission activities, and their spatial position, we analyzed the hydraulic fracturing process of tight sandstone under high differential stress in detail. The experimental results illuminate the fracture evolution processes of hydraulic fracturing. The competition between stress-induced dilatancy and fluid flow was observed during water injection. Moreover, the results prove that the “seismic pump” mode occurs in the dry region, while the “dilation hardening” and “seismic pump” modes occur simultaneously in the partially saturated region, that is to say, the hydraulic conditions dominate the failure mode of the rock.
- Published
- 2021
19. Predicting the Molecular Mechanism of 'Angong Niuhuang Pills' in the Treatment of COVID-19 Based on Network Pharmacology
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Bing Yu, Peng-yu Chen, Chen Wang, Xin-ge Ke, Ying Zhang, Xue-cheng Xiao, Yanfang Yang, Hezhen Wu, and Chong Yuan
- Subjects
0301 basic medicine ,Pharmacology ,Chinese patent medicine ,Coronavirus disease 2019 (COVID-19) ,Computer science ,Plant Science ,General Medicine ,Computational biology ,GeneCards ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Complementary and alternative medicine ,Interaction network ,Docking (molecular) ,Network pharmacology ,Drug Discovery ,Molecular mechanism ,030217 neurology & neurosurgery ,Systems pharmacology - Abstract
Introduction Angong Niuhuang Pills (AGNH), a Chinese patent medicine recommended in the “Diagnosis and Treatment Plan for COVID-19 (8th Edition),” may be clinically effective in treating COVID-19. The active components and signal pathways of AGNH through network pharmacology have been examined, and its potential mechanisms determined. Methods We screened the components in the Traditional Chinese Medicine Systems Pharmacology (TCMSP) via Drug-like properties (DL) and Oral bioavailability (OB); PharmMapper and GeneCards databases were used to collect components and COVID-19 related targets; KEGG pathway annotation and GO bioinformatics analysis were based on KOBAS3.0 database; “herb-components-targets-pathways” (H-C-T-P) network and protein-protein interaction network (PPI) were constructed by Cytoscape 3.6.1 software and STRING 10.5 database; we utilized virtual molecular docking to predict the binding ability of the active components and key proteins. Results A total of 87 components and 40 targets were screened in AGNH. The molecular docking results showed that the docking scores of the top 3 active components and the targets were all greater than 90. Conclusion Through network pharmacology research, we found that moslosooflavone, oroxylin A, and salvigenin in AGNH can combine with ACE2 and 3CL, and then are involved in the MAPK and JAK-STAT signaling pathways. Finally, it is suggested that AGNH may have a role in the treatment of COVID-19.
- Published
- 2021
20. Investigation of Anti-SARS, MERS, and COVID-19 Effect of Jinhua Qinggan Granules Based on a Network Pharmacology and Molecular Docking Approach
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Hezhen Wu, Yunfeng Yao, Ying Zhang, and Yanfang Yang
- Subjects
Pharmacology ,0303 health sciences ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Plant Science ,General Medicine ,Biology ,medicine.disease_cause ,Virology ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,medicine ,030304 developmental biology ,Coronavirus - Abstract
Objective Jinhua Qinggan Granules (JQGs) have achieved certain results in the prevention and treatment of COVID-19 in China during this coronavirus storm. In this study, we aimed to analyze the common mechanisms of JQG in the treatment of coronavirus-induced diseases, such as severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and COVID-19 via network pharmacology and molecular docking. Methods The active compounds of JQG were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. The common targets associated with these 3 diseases were screened from GeneCards database. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of JQG’s core targets were analyzed using The Database for Annotation, Visualization, and Integrated Discovery and KOBAS 3.0 system. Further, the protein-protein interaction network was built using STRING database. The compound-target- signaling pathway network was constructed using Cytoscape 3.7.2. The core components of JQG were docked with core targets, COVID-19 coronavirus 3 Cl hydrolase, and angiotensin-converting enzyme 2 (ACE2) via Discovery Studio 2016 software. Results A total of 139 active compounds, 50 core targets, and 122 signaling pathways were screened out. The results of molecular docking showed that arctiin and linarin had a higher docking score with 3 Cl, ACE2, and core targets of JQH for antiviral effect. Conclusion The potential mechanism of action of JHQ in the treatment of MERS, SARS, and COVID-19 may be associated with the regulation of genes co-expressed with ACE2 and immune- related signaling pathways.
- Published
- 2021
21. Network pharmacology and molecular docking reveal the mechanism of Scopoletin against non-small cell lung cancer
- Author
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Hezhen Wu, Bing Yu, Chong Yuan, Yanfang Yang, Fei Wang, Pengtao You, Xin-ge Ke, Meng-Heng Wang, and Peng-yu Chen
- Subjects
0301 basic medicine ,China ,030226 pharmacology & pharmacy ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Scopoletin ,medicine ,Humans ,Protein Interaction Maps ,Medicine, Chinese Traditional ,General Pharmacology, Toxicology and Pharmaceutics ,Lung cancer ,PI3K/AKT/mTOR pathway ,Chemistry ,Cell growth ,Hep G2 Cells ,General Medicine ,HCT116 Cells ,medicine.disease ,respiratory tract diseases ,Molecular Docking Simulation ,030104 developmental biology ,Mechanism of action ,A549 Cells ,Cell culture ,Cancer cell ,Cancer research ,Signal transduction ,medicine.symptom ,Drugs, Chinese Herbal ,Signal Transduction - Abstract
Scopoletin is a natural anticarcinogenic and antiviral coumarin component. Many studies have proved its anti-cancer effect, and after the preliminary screening of this study, Scopoletin had the best inhibitory effect on Non-small cell lung cancer (NSCLC). But its mechanism for treating NSCLC is still unclear. Therefore, network pharmacology and molecular docking technology were used to explore the potential anti-NSCLC targets and pathways of Scopoletin. The results were verified in vitro.First, Scopoletin was isolated from Fennel and screened to conduct cell proliferation assay on Human lung cancer cell line A549, Human colon cancer cell line HCT-116 and Human hepatoma cell line HepG2 respectively, through the MTT test. Then, the key targets and related pathways were screened through Protein-protein Interaction (PPI) network and "component-target-pathway" (C-TP) network constructed by network pharmacology. And the key targets were selected to dock with Scopoletin via molecular docking. A549 and Human normal lung epithelial cell BEAS-2B were used to verify the results, finally.Through MTT, A549 was chosen as the test cancer cell. From network pharmacology, 16 targets, 27 signaling pathways and 16 GO items were obtained (P 0.05). The results of PPI network and molecular docking showed that EGFR, BRAF and AKT1 were the key targets of Scopoletin against NSCLC, which were consistent with the western-blot results.Through network pharmacology, molecular docking and experiments in vitro, Scopoletin was verified to against NSCLC through RAS-RAF-MEK-ERK pathway and PI3K/AKT pathway.
- Published
- 2021
22. Determination of Alkaloid Contents in Various Tissues of Coptis Chinensis Franch. by Reversed Phase-High Performance Liquid Chromatography and Ultraviolet Spectrophotometry
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Fangping Li, Xin Liu, Hezhen Wu, Yanfang Yang, Meng Deng, and Jingling Peng
- Subjects
Jatrorrhizine ,Coptisine ,01 natural sciences ,High-performance liquid chromatography ,Analytical Chemistry ,chemistry.chemical_compound ,Alkaloids ,Limit of Detection ,Chromatography, High Pressure Liquid ,Detection limit ,Chromatography ,biology ,010405 organic chemistry ,fungi ,010401 analytical chemistry ,Reproducibility of Results ,Xylem ,General Medicine ,Coptis chinensis ,biology.organism_classification ,0104 chemical sciences ,chemistry ,Calibration ,Linear Models ,Spectrophotometry, Ultraviolet ,Phloem ,Plant Structures ,Magnoflorine ,Coptis - Abstract
A simple and intuitive method for optimizing the chemical constituents of Coptis Chinensis Franch. is important to assess its quality and clinical efficacy. An high performance liquid chromatography and ultraviolet spectrophotometry method was developed for the determination of berberine hydrochloride, palmatine chloride, jatrorrhizine hydrochloride, epiberberine, coptisine, columbamine and magnoflorine in various tissues (i.e., phloem, xylem and medulla) and rizhome of C. Chinensis Franch. The transection of rhizome from outside-in includes cork layer, cortex, phloem, cambium, xylem and medulla. Cork layer consists of dead cells, and therefore is not of any research significance. Cortex, phloem and cambium were almost impossible to separate, therefore they were studied as a whole in our experiments. They were collectively referred to as "phloem". The analytes were separated on a Gemini-NX C18 (250 mm × 4.6 mm, 5 μm) reversed phase column using a gradient elution of acetonitrile-0.03 mol/L ammonium acetate solution (containing 0.1% triethylamine and 0.6% ammonium hydroxide) as the mobile phase at a flow rate of 1.0 mL/min and UV detection at 270 nm. The method allowing the simultaneous quantification of seven major active constituents was optimized and validated for linearity, precision, accuracy, limits of detection (LOD) and quantification. The LOD ranged from 0.102 to 0.651 mg/mL (r ≥ 0.9993). Accuracy, precision and recovery were all within the required limits. The average recovery was between 100.14% and 102.75% and the relative standard deviations were
- Published
- 2017
23. 14-Deoxycoleon U-induced endoplasmic reticulum stress-mediated apoptosis, autophagy, and cell cycle arrest in lung adenocarcinoma
- Author
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Xiaozhi, Peng, Yijun, Tu, San, Fu, Yu, Xia, Chaozhi, Ma, Yanfang, Yang, Hezhen, Wu, Yanwen, Liu, and Pengtao, You
- Subjects
endoplasmic reticulum ,autophagy ,apoptosis ,cell cycle ,14-Deoxycoleon U ,Original Research - Abstract
Objective 14-Deoxycoleon U is a natural abietane-type diterpene and exerts an inhibitory effect on tumor cells proliferation, which suggests that 14-Deoxycoleon U may be a potent anti-cancerous lead compound for lung cancer treatment. This study was to evaluate potential of 14-Deoxycoleon U to treat lung adenocarcinoma in vitro and in vivo. Methods In the present study, the cell viability and apoptosis morphology of 14-Deoxycoleon U-treated A549 and LLC cells were explored using cell counting kit-8 assay and Hoechst 33258 staining. Then, the protein expressions about apoptosis, endoplasmic reticulum (ER) stress, autophagy and cell cycle were measured using Western blot. The autophagosome formation of 14-Deoxycoleon U-treated A549 cells was visualized using a confocal microscopy. LLC lung adenocarcinoma model was established. Results The results indicated that 14-Deoxycoleon U significantly inhibited A549 and LLC cell proliferation in a dose-dependent manner via caspase-dependent apoptosis. Furthermore, apoptosis of both cells was mediated by 14-Deoxycoleon U-induced ER stress. In addition, 14-Deoxycoleon U-induced A549 and LLC cell autophagy, thus promoting their death. Moreover, 14-Deoxycoleon U-induced cell cycle arrest in both cells via inhibition of cyclin D3, cyclin-dependent kinase 6, CDC2 and up-regulation of p21. In vivo results showed that administration of 14-Deoxycoleon U significantly suppressed LLC growth and adverse effects of 14-Deoxycoleon U on organs might be lower than of adriamycin. Conclusion Overall, our results demonstrated that 14-Deoxycoleon U represses in vitro and in vivo growth of lung adenocarcinoma through ER stress-mediated apoptosis accompanied by autophagy and cell cycle arrest.
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- 2019
24. Network pharmacology-based research on the active component and mechanism of the antihepatoma effect of Rubia cordifolia L
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Meide Zhang, Xiong Weichen, Xiong Yiyi, Yunfeng Yao, Hezhen Wu, and Yanfang Yang
- Subjects
0301 basic medicine ,Carcinoma, Hepatocellular ,AKT1 ,Computational biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Rubia cordifolia ,Biomarkers, Tumor ,Humans ,Gene Regulatory Networks ,Epidermal growth factor receptor ,Protein kinase A ,Molecular Biology ,chemistry.chemical_classification ,biology ,Kinase ,Plant Extracts ,Gene Expression Profiling ,Liver Neoplasms ,Rubia ,Computational Biology ,Cell Biology ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Enzyme ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Pharmacophore ,Signal transduction - Abstract
Rubia cordifolia L. is widely used in Asia and its antihepatoma effect has been proved by in vitro and in vivo experiments. However, there are few studies on its specific mechanism. In the present study, the network pharmacology method was used to construct the component/target/pathway molecular regulatory network for the antihepatoma effect of Rubia cordifolia L. to explore the effective components of Rubia cordifolia L. and its potential mechanism. The chemical components of Rubia cordifolia L. were identified through literature and databases, and the components were evaluated and screened by drug likeness and pharmacokinetic characteristics (ADMET). The targets of active components were predicted according to the reverse pharmacophore matching model. The hepatic carcinoma-related genes were found in databases, and antihepatoma-related gene targets were selected through comparison. The functions of target genes and related pathways were analyzed and screened using the Database for Annotation, Visualization and Integrated Discovery, and the component/target/pathways network of antihepatoma effect of Rubia cordifolia L. was constructed using Cytoscape software. Finally, 16 active compounds were screened from Rubia cordifolia L., and 39 gene targets, including AKT1, mitogen-activated protein kinase 1, and epidermal growth factor receptor, were involved. Rubia cordifolia L. also affected the hepatitis B, phosphoinositide-3-kinase-protein kinase B, and mitogen-activated protein kinase signaling pathways. Many direct-acting tumor-related signaling pathways and indirect-acting hepatitis pathways inhibit the generation of liver cancer. The present study provided a scientific basis for further elucidating the mechanism of Rubia cordifolia L. against liver cancer.
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- 2018
25. Preliminary Analysis of the Therapeutic Mechanism of Feiluoning in Convalescent Patients With COVID-19
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Hezhen Wu, Yanfang Yang, Ying Zhang, Maolin Hong, Zongchao Hong, and Bo Liu
- Subjects
Pharmacology ,0303 health sciences ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Mechanism (biology) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Plant Science ,General Medicine ,medicine.disease ,Pulmonary function testing ,Preliminary analysis ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Drug Discovery ,Pulmonary fibrosis ,Immunology ,medicine ,Respiratory system ,business ,030304 developmental biology - Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), is often accompanied by injury to pulmonary function and pulmonary fibrosis. Feiluoning (FLN) is a new Chinese medicine prescription which is available for the treatment of severe and critical convalescence of COVID-19 patients. FLN also has a positive effect on pulmonary function injury and pulmonary fibrosis. We explored the potential mechanism of FLN’s effect on the convalescent treatment of COVID-19. According to the pharmacodynamic activity parameters, we screened the active chemical constituents of FLN by comparing the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. The Uniprot database was used to querying the corresponding target genes, and Cytoscape 3.6.1 was used to construct a herb-compound-target network. Protein interaction analysis, target gene function enrichment analysis, and signal pathway analysis were performed using the STRING, DAVID, and Kyoto Encyclopedia of Genes and Genomes pathway databases. Molecular docking was used to predict the binding capacity of the core compound with COVID-19 hydrolase 3 Cl and angiotensin-converting enzyme 2 (ACE2). The herb-compound-target network was successfully constructed and key targets identified, including prostaglandin G/H synthase 2, estrogen receptor 1, heat shock protein HSP 90, and androgen receptor. The major affected metabolic pathways were pathways in cancer, pancreatic cancer, nonsmall cell lung cancer, and toll-like receptor signaling. The core compounds of FLN, including quercetin, luteolin, kaempferol, and stigmasterol, could strongly bind to COVID-19 3 Cl hydrolase, and other compounds, including 7-O-methylisomucronulatol and medicocarpin, could strongly bind to ACE2. Thus, it is predicted that FLN has the characteristics of a multicomponent, multitarget, and multichannel overall control compound. FLN’s mechanism of action in the treatment of COVID-19 may be associated with the regulation of inflammation and immune-related signaling pathways, and the influence of COVID-19 3 Cl hydrolase binding ability.
- Published
- 2020
26. Network Pharmacology Integrated Molecular Docking Reveals the Anti-COVID-19 Mechanism of Xingnaojing Injection
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Peng-yu Chen, Chong Yuan, Bing Yu, Hezhen Wu, Ning Lin, Ying Zhang, Xin-ge Ke, and Yanfang Yang
- Subjects
Pharmacology ,0303 health sciences ,Coronavirus disease 2019 (COVID-19) ,Chemistry ,Mechanism (biology) ,Active components ,Plant Science ,General Medicine ,Computational biology ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,Clinical treatment ,030304 developmental biology - Abstract
In the process of fighting against COVID-19 in China, Xingnaojing injection has been recommended for its clinical treatment, but the information about its active components and mechanism is still lacking. Therefore, in this work, using network pharmacology and molecular docking, we studied the active components of Xingnaojing injection having anti-COVID-19 properties. Using the DL parameter, TCMSP and CNKI databases were used to screen the active components of the Xingnaojing injection. Then, the SwissTargetPrediction webserver was used to collect the corresponding gene targets, and the gene targets related to COVID-19 were searched in the Genecards database. The DAVID database was used to enrich the function of gene targets, and the KOBAS3.0 database for the annotation of related KEGG pathways. The “components–targets–pathways” network of Xingnaojing injection was constructed with Cytoscape 3.6.1 software. The protein–protein interaction networks were analyzed using the String database. Specific proteins, SARS-COV-2 3 Cl, ACE2, and the active components were imported into Discovery Studio 2016 Client for molecular docking studies. From the Xingnaojing injection, a total of 58 active components, including Divanillalaceton and Q27139023, were screened. These were linked to 53 gene targets including mitogen-activated protein kinase 1 (MAPK1), tumor necrosis factorTNF, epidermal growth factor receptor, MAPK3, and 196 signaling pathways related to COVID-19, such as apoptosis, C-type lectin receptor signaling pathway, and hypoxia-inducible factor 1 signaling pathway. Furthermore, molecular docking studies were performed to study potential binding between the key targets and selected active components. Xingnaojing injection exhibits anti-COVID-19 effects via multiple components, multiple targets, and multiple pathways. These results set a scientific basis for further elucidation of the anti-COVID-19 mechanism of Xingnaojing injection.
- Published
- 2020
27. Network Pharmacology Integrated Molecular Docking Analysis of Potential Common Mechanisms of Shu-Feng-Jie-Du Capsule in the Treatment of SARS, MERS, and COVID-19
- Author
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Bing Yu, Yi Xie, Yanfang Yang, Hezhen Wu, Zongchao Hong, Chong Yuan, Ying Zhang, and Zixin Yuan
- Subjects
Pharmacology ,0303 health sciences ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Molecular Docking Analysis ,Plant Science ,General Medicine ,medicine.disease_cause ,Virology ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,medicine ,business ,030304 developmental biology ,Coronavirus - Abstract
Shu-Feng-Jie-Du Capsules (SFJDCs) have been clinically proven to have a good therapeutic effect on COVID-19 in China. This study aimed to analyze the common mechanisms of SFJDC in the treatment of severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and COVID-19 via network pharmacology and molecular docking. We further explored the potential application value of SFJDC in the treatment of coronavirus infection. All components of SFJDC were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. The viral associated targets of the active components were forecast using the Pharmmapper database and GeneCards. The Database for Annotation, Visualization, and Integrated Discovery and KOBAS 3.0 system were used for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of SFJDC’s core targets. Further, the protein–protein interaction network was built using STRING database. The herb–component network and component–target–pathway network were constructed using Cytoscape 3.7.2. The core active components of SFJDC were docked with core targets and COVID-19 coronavirus 3 Cl hydrolase and angiotensin-converting enzyme 2 (ACE2) via Discovery Studio 2016 software. A total of 110 active components were filtered from SFJDC, with 47 core targets, including epidermal growth factor receptor, mitogen-activated protein kinase 1, mitogen-activated protein kinase 3, and interleukin 6. There were 416 GO items in the GO enrichment analysis ( P < .05) and 57 signaling pathways ( P < .05) in KEGG, mainly including pathways in cancer, pancreatic cancer, colorectal cancer, apoptosis, and neurotrophin signaling pathway, among others. The results of molecular docking showed that luteolin and rhein had a higher docking score with 3 Cl, ACE2, and core targets of SFJDC for antiviral effect. SFJDC is characterized by multicomponent, multitarget, and multisignaling pathways for the treatment of coronavirus infection. The mechanism of action of SFJDC in the treatment of MERS, SARS, and COVID-19 may be associated with the regulation of genes coexpressed with ACE2 and immune- related signaling pathways.
- Published
- 2020
28. Quercitrin ameliorates the development of systemic lupus erythematosus-like disease in a chronic graft-versus-host murine model
- Author
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Laurence Morel, Youxiu Zhong, Yanfang Yang, Qun Wei, Hu Li, Wei Li, Hezhen Wu, Mu Zhang, and Mengqi Wang
- Subjects
CD4-Positive T-Lymphocytes ,Lipopolysaccharides ,0301 basic medicine ,MAP Kinase Signaling System ,Physiology ,T cell ,Lupus nephritis ,Graft vs Host Disease ,Inflammation ,GATA3 Transcription Factor ,Lymphocyte Activation ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Immune system ,immune system diseases ,Animals ,Lupus Erythematosus, Systemic ,Medicine ,Autoantibodies ,business.industry ,Autoantibody ,medicine.disease ,Quercitrin ,Disease Models, Animal ,Proteinuria ,RAW 264.7 Cells ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Mice, Inbred DBA ,030220 oncology & carcinogenesis ,Immunology ,Cytokines ,Female ,Quercetin ,medicine.symptom ,business ,Nephritis ,Spleen - Abstract
Systemic lupus erythematosus (SLE) is a serious disorder of immune regulation characterized by overproduction of autoantibodies, lupus nephritis, CD4+ T cell aberrant activation, and immune complex-mediated inflammation. The chronic graft vs. host disease (cGVHD) mouse model is a well-established model of SLE. Quercitrin is a natural compound found in Tartary buckwheat with a potential anti-inflammatory effect that is used to treat heart and vascular conditions. In our previous study, we determined that quercitrin is an immunosuppressant with beneficial effects in mouse models of immune diseases. We hypothesized that quercitrin could prevent lupus nephritis in the cGVHD mouse model by decreasing the production of autoantibodies and inflammatory cytokines, and reducing immune cell activation. cGVHD was induced by injecting DBA/2 spleen cells into the tail vein of BDF1 mice. The cGVHD mice exhibited significant proteinuria, which is a marker of nephritis. Quercitrin decreased the number of serum antibodies, CD4+ T cell activation, as well as the expression levels of T-bet, GATA-3, and selected cytokines. Moreover, quercitrin treatment decreased the expression of inflammatory genes and cytokines in the kidney, as well as in peritoneal macrophages. In addition, quercitrin inhibited LPS-induced cytokines as well as the phosphorylation of ERK, p38 MAPK, and JNK in Raw264.7 cells. Overall, quercitrin ameliorated the symptoms of lupus nephritis in the cGVHD mouse model, which may be due to the inhibition of CD4 T cell activation and anti-inflammatory effects on macrophages.
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- 2016
29. Study of Components and Mechanism of Juechuang Against Platelet Aggregation Based on Network Pharmacology
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Zhou-Tao Xie, Hezhen Wu, Xiong Yiyi, Bo Liu, and Yanfang Yang
- Subjects
Pharmacology ,0303 health sciences ,Platelet aggregation ,Mechanism (biology) ,Ethyl acetate ,Plant Science ,General Medicine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Complementary and alternative medicine ,chemistry ,Biochemistry ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,Platelet ,030304 developmental biology - Abstract
Juechuang, a traditional Chinese herbal medicine, is originated from Rostellularia procumbens (L.) Nees. Many studies have shown that the ethyl acetate extract from Juechaung may inhibit platelet aggregation. However, the antiplatelet aggregation mechanism of Juechuang requires more systematic research. In this article, network pharmacology was used to explore the antiplatelet aggregation components and its antiplatelet aggregation mechanism. Different components were evaluated and screened by pharmacokinetic characteristics. The potential targets of active ingredients were predicted by a reverse pharmacophore matching method, and the targets were screened according to targets related to antiplatelet aggregation in the GeneCards database. Thus, an interaction network of component-target-pathway of Juechuang was generated using Cytoscape 3.2.1. software. Furthermore, the binding energy of relevant active components with key targets was calculated using a Lamarck genetic algorithm in the molecular docking calculations. Finally, the study identified 28 potentially active ingredients in Juechuang, providing further evidence that the active ingredients act on 277 targets, and 38 protein targets related to antiplatelet aggregation were screened. Through the Kyoto encyclopedia of genes and genome pathway enrichment analysis, we found that the mechanism of antiplatelet aggregation may be related to the Ras signaling pathway, platelet activation signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, etc. Via molecular docking of 2 targets, non-receptor tyrosine kinases(SRC) and MAPK were selected for molecular docking. By comparing the molecular docking results of Chinensinaphthol, Taiwanin E, Tuberculatin, Cycloeucalenol, and Justicidin B to the control drug, we found that those test molecules combined with targets and lead to high binding activity. These molecular docking results were also consistent with the literature values, and they helped identify the active ingredients and assured the reliability of the network analysis. This study may further provide a reference for the systematic study of the pharmacodynamic effect and the antiplatelet aggregation mechanism of Juechuang.
- Published
- 2020
30. Network Pharmacology Integrated Molecular Docking Reveals the Anti-COVID-19 Mechanism of Qing-Fei-Da-Yuan Granules
- Author
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Xueyun Duan, Hezhen Wu, Zongchao Hong, Yang Yanfang, and Songtao Wu
- Subjects
0301 basic medicine ,Pharmacology ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Plant Science ,General Medicine ,Biology ,medicine.disease_cause ,Virology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Complementary and alternative medicine ,Mechanism of action ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,medicine ,Viral disease ,medicine.symptom ,Coronavirus - Abstract
Coronavirus disease (COVID-19), caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a highly infectious viral disease. Clinical observations have shown that Qing-Fei-Da-Yuan (QFDY) granules have good anti-COVID-19 effects, but the underlying molecular mechanisms are unclear. In this study, we explored the potential mechanism of QFDY with regard to its anti-COVID-19 effect. We first screened the active chemical constituents of QFDY based on the pharmacodynamic activity parameters, followed by screening with the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. The Uniprot database was used for querying the corresponding genes of the target, and Cyoscape 3.6.1 software was used to construct the network of herb-compound-target. Protein interaction analysis, target gene function enrichment analysis, and signal pathway analysis were performed via STRING database, Database for Annotation, Visualization, and Integrated Discovery, and KEGG Pathway database. Molecular docking was used to predict the binding capacity of the core compound with COVID-19 hydrolase 3CL and angiotensin converting enzyme 2 (ACE2). The results showed that a network of herb-compound-target was successfully constructed, with key targets involving PTGS2, HSP90AA1, CAMKK2, NCOA2, and ESR1. Major metabolic pathways affected were those in cancer, procancer, nonsmall cell lung cancer, and apoptosis. The core compounds, such as quercetin, luteolin, and naringenin, showed a strong binding ability with COVID-19 3CL hydrolase; compounds such as anemasaponin C and medicocarpin showed a strong binding ability with ACE2. Thus, it is predicted that QFDY has the characteristics for multicomponent, multitarget, and multichannel overall control. The mechanism of action of QFDY in the treatment of COVID-19 may be associated with the regulation of genes co-expressed with ACE2, the regulation of inflammation and immune-related signaling pathways, and the influence of COVID-19 3CL hydrolase and ACE2 binding ability.
- Published
- 2020
31. Justicidin B Inhibits PDGF-BB-Induced Proliferation and ECM Accumulation in Mesangial Cells via Nrf2/HO-1 and Akt/mTOR Signaling Pathway
- Author
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Yu Zhang, Shan-Shan Zhou, Hezhen Wu, Zhongzhu Ai, Meng-fan Wang, Yanfang Yang, Song-Tao Wu, and Zongchao Hong
- Subjects
Pharmacology ,0303 health sciences ,biology ,Chemistry ,MTOR signaling pathway ,Inflammation ,Plant Science ,General Medicine ,medicine.disease ,Cell biology ,Extracellular matrix ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,Mesangial cell proliferation ,030220 oncology & carcinogenesis ,Nrf2 ho 1 ,Drug Discovery ,medicine ,biology.protein ,Mesangial proliferative glomerulonephritis ,medicine.symptom ,Protein kinase B ,Platelet-derived growth factor receptor ,030304 developmental biology - Abstract
Mesangial proliferative glomerulonephritis (MsPGN) is characterized by mesangial cell proliferation, inflammation, and extracellular matrix deposition in the mesangial area, which develops into glomerulosclerosis and contributes to end-stage renal disease. Justicidin B is a bioactive compound isolated from Justicia procumbens L., a traditional herbal remedy that reduces proteinuria in nephritis. However, the mechanism of Justicidin B’s therapeutic effect on MsPGN remains unclear. This study was aimed to explore the positive effect of Justicidin B on MsPGN. The results showed that Justicidin B attenuated the proliferation induced by platelet-derived growth factor-BB (PDGF-BB) in MCs and blocked cell cycle progression. Likewise, inflammatory factors, including monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor alpha (TNF-α), in MCs were decreased after treatment with Justicidin B. In addition, Justicidin B exhibited antioxidant activity in PDGF-BB-induced MCs, shown by the decreased production of malondialdehyde and T-AOC, and increased the expression of superoxide dismutase. Besides, Justicidin B suppressed extracellular matrix (ECM) deposition by reducing the protein levels of collagen IV and fibronectin. Furthermore, we found that Justicidin B significantly inhibited activation of the Akt/mammalian target of rapamycin (mTOR) signaling pathway in MCs induced by PDGF-BB, but enhanced the levels of proteins in the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. Taken together, Justicidin B prevented PDGF-BB-induced proliferation, inflammation, oxidative stress, and ECM accumulation via regulating the activation of the Nrf2/HO-1 pathway and the Akt/mTOR signaling pathway.
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- 2020
32. Network Pharmacology Research and Preliminary Verification of Gegen Qinlian Decoction for the Treatment of Non-Alcoholic Fatty Liver Disease
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Xiong Yiyi, Hao Pengfei, Hezhen Wu, and Yanfang Yang
- Subjects
Pharmacology ,0303 health sciences ,business.industry ,Cellular pathways ,Fatty liver ,Decoction ,Non alcoholic ,Plant Science ,General Medicine ,Traditional Chinese medicine ,Disease ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Network pharmacology ,Pharmacodynamics ,Drug Discovery ,medicine ,business ,030304 developmental biology - Abstract
Gegen Qinlian decoction (GQD) is a traditional Chinese medicine that is used to treat non-alcoholic fatty liver disease (NAFLD) in the clinic. The pharmacodynamics and cellular pathways governing the effects of GQD on NAFLD remain undefined. In this study, we investigated GQD pharmacology through assessment of its chemical constituents and evaluated and screened its components using drug likeness, pharmacokinetic characteristics (absorption, distribution, metabolism, excretion, and toxicity), and appropriate compensation mechanisms. We performed predictions of the active GQD ingredients based on reverse pharmacophore matching and compared multiple NAFLD-related genes to determine potential GQD targets. Molecular docking experiments of the active components were performed to reveal cellular targets. Annotation analysis of both target genes and related pathways were assessed through the DAVID database. Cytoscape software was used to construct a “component-target-path” network for the treatment of NAFLD by GQD. Through data analysis, 9 active GQD substances and 10 targets related to NAFLD encompassing 4 cellular pathways were identified. Data were verified through enzyme-linked immunosorbent assay and Western blot analysis. These findings provide new references for the network pharmacology of Chinese medicinal compounds and NAFLD treatment.
- Published
- 2020
33. Extracts of Coleus forskohlii relieves cough and asthma symptoms via modulating inflammation and the extracellular matrix
- Author
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Pengtao You, Daquan Xue, Yu Xia, Yanwen Liu, Hezhen Wu, Dan Liu, Yijun Tu, Hanxiong Dan, Liyuan Zou, Yanfang Yang, and Chaozhi Ma
- Subjects
0301 basic medicine ,Male ,food.ingredient ,Guinea Pigs ,H&E stain ,Inflammation ,Pharmacology ,Biochemistry ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,food ,medicine ,Animals ,Plectranthus ,Molecular Biology ,medicine.diagnostic_test ,biology ,business.industry ,Plant Extracts ,Interleukin ,Coleus ,Cell Biology ,respiratory system ,Tissue inhibitor of metalloproteinase ,Asthma ,respiratory tract diseases ,Extracellular Matrix ,Rats ,Ovalbumin ,030104 developmental biology ,Bronchoalveolar lavage ,chemistry ,Cough ,030220 oncology & carcinogenesis ,biology.protein ,Airway Remodeling ,Cytokines ,medicine.symptom ,business ,Histamine - Abstract
Asthma is characterized by airway inflammatory infiltration, which leads to airway remodeling and airway hyperreactivity. Coleus forskohlii (CFK) has been used to treat asthma, however, the mechanism involved is not clear. To explore the antiasthma mechanism of extracts of Coleus forskohlii (ECFK), guinea pigs were administered with a spray of phosphoric acid histamine, and rats were sensitized with ovalbumin (OVA). Hematoxylin and eosin staining (H&E) were used to evaluate pathological changes in lung tissue. Enzyme-linked immunosorbent assay (ELISA) was used to determine cytokine levels in serum and bronchoalveolar lavage fluid (BALF). Immunohistochemistry and Western blot analysis were used to assess the expression of intercellular cell adhesion molecule-1 (ICAM-1), phosphorylation of p65 (p-p65), matrix metallopeptidase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1). After ECFK treatment, the asthma incubation period of guinea pigs was significantly prolonged. The H&E results showed that the number of eosinophils in the 12.8 g/kg ECFK group was significantly lower when compared with the control group. Moreover, ELISA results demonstrated that interleukin (IL)-4, IL-5, and IL-17 in serum and BALF were significantly decreased, and interferon-γ (IFN-γ) and IL-10 were increased after ECFK treatment. In addition, ECFK treatment resulted in downregulation of ICAM-1, p-p65, MMP-9, and TIMP-1 in lung tissue after being sensitized by OVA. In conclusion, our findings indicated that ECFK significantly alleviated OVA-induced inflammatory infiltration and airway remodeling in asthma. This study laid a theoretical foundation for the clinical use of ECFK.
- Published
- 2018
34. Predicting the Mechanism of the Analgesic Property of Yanhusuo Based on Network Pharmacology
- Author
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Hezhen Wu, Xiong Yiyi, Wen-Ping Xiao, and Yanfang Yang
- Subjects
Pharmacology ,0303 health sciences ,biology ,Mechanism (biology) ,Chemistry ,Analgesic ,Plant Science ,General Medicine ,Corydalis ,biology.organism_classification ,03 medical and health sciences ,0302 clinical medicine ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Network pharmacology ,Drug Discovery ,030304 developmental biology - Abstract
Yanhusuo (Corydalis Rhizoma) extracts are widely used for the treatment of pain and inflammation. The effects of Yanhusuo in pain assays were assessed in a few studies. However, there are few studies on its analgesic mechanism. In this paper, network pharmacology was used to explore the analgesic components of Yanhusuo and its analgesic mechanism. The active components of Yanhusuo were screened by TCMSP database, combined with literature data. PharmMapper and GeneCards databases were used for screening the analgesic targets of the components. The protein interaction network diagram was drawn by String database and Cytoscape software, the gene ontology and KEGG pathway analyses of the target were performed by DAVID database, and the component–target–pathway interaction network diagram was further drawn by Cytoscape3.6.1 software. System Dock Web Site verified the molecular docking among components and targets. Finally, an interaction network of the component–target–pathway of Yanhusuo was constructed, and the functions and pathways were analyzed for preliminarily investigating the mechanism of Yanhusuo in analgesia. The results showed that the active components of analgesic in Yanhusuo were Corynoline, 13-methylpalmatrubine, dehydrocorydaline, saulatine, 2,3,9,10-tetramethoxy-13-methyl-5,6-dihydroisoquinolino[2,1-b]isoquinolin-8-on-e, and Capaurine. The mechanisms were involved in metabolic pathways, PI3k-Akt signaling pathway, pathways in cancer, and so on. The top 3 targets were NOS3, glucose-6-phosphate dehydrogenase, and glucose-6-phosphate isomerase in components-target-pathways network, and they were all enriched in metabolic pathways. Meanwhile the molecular docking showed that there was a high binding activity between the 6 components and the important target proteins, as a further certification for the subsequent network analysis. This study reveals the relationship of the components, targets, and pathways of active components in Yanhusuo, and provides new ideas and methods for further research on the analgesic mechanism of Yanhusuo.
- Published
- 2019
35. Isogarcinol Extracted from Garcinia mangostana L. Ameliorates Systemic Lupus Erythematosus-like Disease in a Murine Model
- Author
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Youxiu Zhong, Hezhen Wu, Mu Zhang, Juren Cen, Yanfang Yang, Mengqi Wang, Qun Wei, Wei Li, and Hu Li
- Subjects
food.ingredient ,T-Lymphocytes ,Graft vs Host Disease ,Inflammation ,Disease ,Garcinia mangostana ,Mice ,food ,Downregulation and upregulation ,Oral administration ,medicine ,Animals ,Humans ,Lupus Erythematosus, Systemic ,Proteinuria ,biology ,Plant Extracts ,Terpenes ,business.industry ,Macrophages ,General Chemistry ,Mice, Inbred C57BL ,Disease Models, Animal ,Mechanism of action ,Mice, Inbred DBA ,Immunology ,biology.protein ,Cytokines ,Female ,medicine.symptom ,Antibody ,General Agricultural and Biological Sciences ,business ,Immunosuppressive Agents - Abstract
Isogarcinol is a new immunosuppressant that we extracted from Garcinia mangostana L. In the present study, we elucidate its beneficial effect in chronic graft-versus-host disease (cGVHD) in mice -- a model for systemic lupus erythematosus (SLE) in human. The oral administration of 60 mg/kg isogarcinol significantly reduced proteinuria, corrected the abnormal serum biochemical indicator, and decreased the amount of serum antibodies and lowered the renal histopathology score. In addition, isogarcinol alleviated the abnormal activation of CD4 T cells and decreased the expression of inflammatory genes and cytokines in the kidneys and peritoneal macrophages. The mechanism of action of isogarcinol is associated with downregulation of CD4 T cells and inflammatory effects. Therefore, we believe that isogarcinol may be a potential therapeutic drug candidate for future treatment of SLE.
- Published
- 2015
36. Brevilin A induces apoptosis and autophagy of colon adenocarcinoma cell CT26 via mitochondrial pathway and PI3K/AKT/mTOR inactivation
- Author
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Hezhen Wu, Fangping Li, Yanfang Yang, Meng Deng, Jingling Peng, and Pengtao You
- Subjects
0301 basic medicine ,Male ,Cell ,ATG5 ,Antineoplastic Agents ,Apoptosis ,Adenocarcinoma ,03 medical and health sciences ,Mice ,Phosphatidylinositol 3-Kinases ,0302 clinical medicine ,In vivo ,Cell Line, Tumor ,medicine ,Autophagy ,Animals ,Humans ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Pharmacology ,Membrane Potential, Mitochondrial ,Mice, Inbred BALB C ,Chemistry ,TOR Serine-Threonine Kinases ,General Medicine ,Hep G2 Cells ,Mitochondria ,030104 developmental biology ,medicine.anatomical_structure ,A549 Cells ,030220 oncology & carcinogenesis ,Caspases ,Crotonates ,Colonic Neoplasms ,Cancer research ,Phosphorylation ,Proto-Oncogene Proteins c-akt ,Sesquiterpenes ,HeLa Cells ,Signal Transduction - Abstract
Brevilin A is a sesquiterpene lactone isolated from Centipeda minima and possesses inhibitory effects on proliferation of various tumor cells. In this study, Brevilin A inhibitory effect on proliferation and its molecular mechanism of action were investigated both in vivo and in vitro in colon adenocarcinoma CT26 cells. The results indicated that the inhibitory effect of Brevilin A in CT26 proliferation was dose-dependent and this effect was due to apoptosis. Furthermore, Brevilin A increased ROS levels, decreased mitochondrial membrane potential (MMP) and induced apoptosis of CT26 cell in a dose-dependent manner. Apoptosis induced by Brevilin A was higher than that induced by adriamycin under the same dose. Cleaved-caspase-8, cleaved-caspase-9 and cleaved-caspase-3 were up-regulated after Brevilin A treatment, together with an increase of Bax protein expression, while Bcl-2 was reduced. Further investigation revealed that Brevilin A inhibited the phosphorylation of PI3K, AKT and mTOR and promoted the expressions of autophagy-related proteins LC3-II, Beclin1 and Atg5 and consequent formation of autophagosomes, whereas 3-methyladenine (3-MA), a type III PI3K inhibitor, inhibited autophagosomes formation induced by Brevilin A. In vivo investigation suggested that Brevilin A significantly inhibited the growth of CT26 tumor compared to adriamycin and concurrently promoted the expressions of LC3-II and cleaved-caspase-3 in tumor tissues. Our results demonstrated that the anti-tumor activity of Brevilin A was mainly achieved by the induction of cell apoptosis and autophagy, suggesting a promising potential as antitumor drug against colon adenocarcinoma.
- Published
- 2017
37. Sesquiterpene lactone 6-O-angeloylplenolin reverses vincristine resistance by inhibiting YB-1 nuclear translocation in colon carcinoma cells
- Author
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Hezhen Wu, Jianwen Liu, Zhenwen Chen, Changlong Li, and Yanfang Yang
- Subjects
0301 basic medicine ,Cancer Research ,Vincristine ,Oncogene ,Chemistry ,Cell ,ATP-binding cassette transporter ,Cell cycle ,physiological processes ,Molecular medicine ,Multiple drug resistance ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,polycyclic compounds ,medicine ,Cancer research ,neoplasms ,medicine.drug - Abstract
Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy efficacy. In the present study, 6-O-angeloylplenolin repressed the overexpression of ATP binding cassette subfamily B member 1 (MDR1) and increasing the intracellular concentration of anticancer drugs. A reduction in P-glycoprotein expression (encoded by MDR1) was observed in parallel with a decline in mRNA expression in vincristine-resistant HCT (HCT-8/VCR) cells treated with 6-O-angeloylplenolin. In addition, 6-O-angeloylplenolin suppressed the activity of the MDR1 gene promoter. Treatment with 6-O-angeloylplenolin also decreased the amount of the specific protein complex that interacted with the MDR1 gene promoter in HCT-8/VCR cells, potentially leading to the suppression of MDR1 expression. Treatment with 6-O-angeloylplenolin inhibited the nuclear translocation of Y-box binding protein-1 in HCT-8/VCR cells treated with 6-O-angeloylplenolin, contributing to the negative regulation of MDR1. Finally, 6-O-angeloylplenolin reversed VCR resistance in an HCT/VCR xenograft model. In conclusion, 6-O-angeloylplenolin exhibited a MDR-reversing effect by downregulating MDR1 expression and could represent a novel adjuvant agent for chemotherapy.
- Published
- 2017
38. Flavonoids and Tannins from Smilax china L. Rhizome Induce Apoptosis Via Mitochondrial Pathway and MDM2-p53 Signaling in Human Lung Adenocarcinoma Cells
- Author
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Yuhang Wang, Qiwei Hu, Xin Chen, Pengtao You, Yanfang Yang, Dan Liu, Yanwen Liu, Xiaochuan Ye, Song Wang, San Fu, Hezhen Wu, and Yan Luo
- Subjects
0301 basic medicine ,Lung Neoplasms ,Cell Survival ,Apoptosis ,Mitochondrion ,Pharmacology ,Adenocarcinoma ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Tumor Cells, Cultured ,Humans ,A549 cell ,Flavonoids ,medicine.diagnostic_test ,biology ,Dose-Response Relationship, Drug ,Cell growth ,Smilax china ,Proto-Oncogene Proteins c-mdm2 ,General Medicine ,biology.organism_classification ,In vitro ,Mitochondria ,030104 developmental biology ,Complementary and alternative medicine ,Biochemistry ,030220 oncology & carcinogenesis ,Caspases ,Smilax ,Signal transduction ,Tumor Suppressor Protein p53 ,Tannins ,Signal Transduction - Abstract
In vitro evidence indicates that Smilax china L. rhizome (SCR) can inhibit cell proliferation. Therefore, in the present study, we analyzed the effects in vitro of SCR extracts on human lung adenocarcinoma A549 cells. Our results showed that A549 cell growth was inhibited in a dose- and time-dependent manner after treatment with SCR extracts. Total flavonoids and total tannins from SCR induced A549 apoptosis in a dose-dependent manner, as shown by our flow cytometry analysis, which was consistent with the alterations in nuclear morphology we observed. In addition, the total apoptotic rate induced by total tannins was higher than the rate induced by total flavonoids at the same dose. Cleaved-caspase-3 protein levels in A549 cells after treatment with total flavonoids or total tannins were increased in a dose-dependent manner, followed by the activation of caspase-8 and caspase-9, finally triggering to PARP cleavage. Furthermore, total flavonoids and total tannins increased the expression of Bax, decreased the expression of Bcl-2, and promoted cytochrome [Formula: see text] release. Moreover, MDM2 and p-MDM2 proteins were decreased, while p53 and p-p53 proteins were increased, both in a dose-dependent manner, after A549 treatment with total flavonoids and total tannins. Finally, cleaved-caspase-3 protein levels in the total flavonoids or total tannins-treated H1299 (p53 null) and p53-knockdown A549 cells were increased. Our results indicated that total flavonoids and total tannins from SCR exerted a remarkable effect in reducing A549 growth through their action on mitochondrial pathway and disruption of MDM2-p53 balance. Hence, our findings demonstrated a potential application of total flavonoids and total tannins from SCR in the treatment of human lung adenocarcinoma.
- Published
- 2017
39. Isogarcinol Extracted from Garcinia mangostana L. Ameliorates Imiquimod-Induced Psoriasis-like Skin Lesions in Mice
- Author
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Hezhen Wu, Kesheng Han, Hu Li, Shanzao Chen, Yanfang Yang, Qun Wei, and Juren Cen
- Subjects
0301 basic medicine ,food.ingredient ,T cell ,Imiquimod ,Spleen ,Pharmacology ,Interleukin-23 ,T-Lymphocytes, Regulatory ,Garcinia mangostana ,03 medical and health sciences ,Mice ,0302 clinical medicine ,food ,Interferon ,Psoriasis ,medicine ,Animals ,Humans ,Gene ,Skin ,Chemistry ,Plant Extracts ,Tumor Necrosis Factor-alpha ,General Chemistry ,medicine.disease ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Rheumatoid arthritis ,Immunology ,Aminoquinolines ,Interleukin-2 ,Th17 Cells ,Female ,General Agricultural and Biological Sciences ,medicine.drug - Abstract
Isogarcinol (YDIS), a natural compound extracted from Garcinia mangostana L., has a significant immunosuppressive effect on systemic lupus erythematosus and rheumatoid arthritis. This paper reports that it reduced imiquimod-induced psoriasis-like skin lesions in mice. It strongly attenuated the aberrant proliferation and differentiation of keratinocytes. Moreover, the expression of genes involving the interleukin-23 (IL-23)/T-helper 17 (Th17) axis was significantly inhibited in the dorsal skin of the YDIS-treated mice, as was that of the other pro-inflammatory factors TNF-α, IL-2, and even interferon (IFN)-γ. Furthermore, YDIS prevented the abnormal distribution of T cell types and suppressed the differentiation of CD4+ T cells into Th17 cells in the spleens of mice exposed to imiquimod. Interestingly, it elevated numbers of regulatory T cells (Tregs) in the spleen and boosted IL-10 expression in the skin. In agreement with the above, YDIS increased serum IL-10 and reduced serum IL-17. It also caused less...
- Published
- 2017
40. Coleus Forskolin Briq. Extract inhibits inflammatory via NF-kappa B signaling in cough and asthma model
- Author
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Hezhen Wu, Pengtao You, Yanfang Yang, Chaozhi Ma, Xiaochun Ye, Yanwen Liu, and Xin Chen
- Subjects
chemistry.chemical_compound ,food.ingredient ,food ,Forskolin ,chemistry ,Applied Mathematics ,General Mathematics ,Coleus ,Pharmacology ,Asthma model ,NFKB1 - Published
- 2018
41. Cultural regionalization for Coptis chinensis based on 3S technology platform Ⅰ. Study on growth suitability for Coptis chinensis based on ecological factors analysis by Maxent and ArcGIS model
- Author
-
Hong-Ping Song, Hezhen Wu, Xin Liu, Xiao-Bo Zhang, Luqi Huang, and Yanfang Yang
- Subjects
China ,Resource (biology) ,Distribution (economics) ,01 natural sciences ,Alkaloids ,Altitude ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Ecosystem ,Ecology ,biology ,010405 organic chemistry ,business.industry ,Coptis chinensis ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Geography ,Complementary and alternative medicine ,Chemical quality ,Habitat ,Geographic Information Systems ,business ,Rhizome ,Coptis - Abstract
At the urgent request of Coptis chinensis planting,growth suitability as assessment indicators for C. chinensis cultivation was proposed and analyzed in this paper , based on chemical quality determination and ecological fators analysis by Maxent and ArcGIS model. Its potential distribution areas at differernt suitability grade and regionalization map were formulated based on statistical theory and growth suitability theory. The results showed that the most suitable habitats is some parts of Chongqing and Hubei province, such as Shizhu, Lichuan, Wulong, Wuxi, Enshi. There are seven ecological factor is the main ecological factors affect the growth of Coptidis Rhizoma, including altitude, precipitation in February and September and the rise of precipitation and altitude is conducive to the accumulation of total alkaloid content in C. chinensis. Therefore, The results of the study not only illustrates the most suitable for the surroundings of Coptidis Rhizoma, also helpful to further research and practice of cultivation regionalization, wild resource monitoring and large-scale cultivation of traditional Chinese medicine plants.
- Published
- 2016
42. Purification and characterisation of a novel protease from Cordyceps sinensis and determination of the cleavage site motifs using oriented peptide library mixtures
- Author
-
Wang Xinyu, Hezhen Wu, Qun Wei, and Bi Bo
- Subjects
Cordyceps ,Proteases ,Protease ,biology ,medicine.medical_treatment ,Lysine ,General Medicine ,Edible fungus ,Cleavage (embryo) ,biology.organism_classification ,Analytical Chemistry ,Biochemistry ,medicine ,Proline ,Peptide library ,Food Science - Abstract
A novel protease, from the edible fungus Cordyceps sinensis , was purified and characterised. Its cleavage site motifs were determined by oriented peptide library mixtures and validated by synthetic peptides and natural proteins. The protease was purified to homogeneity using anion-exchange chromatography, sieve chromatography, native PAGE and reversion phase chromatography. Its molecular weight, estimated by SDS–PAGE, was approximately 43 kDa. The results of MS-MS, MALDI-TOF MS and de novo sequencing demonstrated that it was a completely new protease. We used oriented peptide library mixtures to determine cleavage site motifs. Cleavage requires lysine at P1 and proline or lysine at P3′. P2 and P1′ also show some preference. A series of synthetic peptides and natural proteins were used to validate the substrate specificity. The protease has special substrate preferences different from other proteases. It also has excellent biochemical properties, which make it able to withstand harsh conditions and suitable for industrialisation and commercialisation.
- Published
- 2011
43. Interaction of glycyrol with calcineurin A studied by spectroscopic methods and docking
- Author
-
Hezhen Wu, Yao Qi, Li-qin Peng, and Qun Wei
- Subjects
Glycerol ,Models, Molecular ,CALCINEURIN A ,Circular dichroism ,Chemistry ,Stereochemistry ,Calcineurin ,Circular Dichroism ,Spectrum Analysis ,Protein subunit ,Clinical Biochemistry ,Cell Biology ,Biochemistry ,Protein Structure, Secondary ,Hydrophobic effect ,Energy Transfer ,Docking (molecular) ,Mutation ,Genetics ,Binding site ,Molecular Biology ,Protein secondary structure - Abstract
We have shown previously that glycyrol has an inhibitory effect on the immune response in mice by reducing calcineurin activity (Li et al., 2010, Pharm Biol 48:1177–1184). Here, we investigated the interaction of glycyrol with calcineurin A (CNA, catalytic A subunit of calcineurin) by spectroscopic methods and docking. We showed that glycyrol binds to CNA via hydrophobic interactions in a ratio of 1:1, and the main binding site is in the catalytic domain of CNA close to the calcineurin B subunit-binding domain. Binding of glycyrol changes the secondary structure of CNA, and this effect may possibly inhibit CN activity. © 2011 IUBMB IUBMB Life, 63(1): 14–20, 2011
- Published
- 2011
44. INFLUENCE OF SCANNING MODES OF CYCLIC VOLTAMMETRY ON THE SURFACE MORPHOLOGY OF POLYANILINE FILMS
- Author
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Zhongping Chen, Hezhen Wu, Jiahao Guo, Daobao Chu, Xiaojuan Yin, and Junhua Chen
- Subjects
chemistry.chemical_compound ,Materials science ,Morphology (linguistics) ,Polymers and Plastics ,chemistry ,Chemical engineering ,General Chemical Engineering ,Polyaniline ,Analytical chemistry ,General Chemistry ,Cyclic voltammetry - Published
- 2009
45. A flavonoid glycoside isolated from Smilax china L. rhizome in vitro anticancer effects on human cancer cell lines
- Author
-
Jian-Wen Liu, Yongping Huang, Guo-Ping Gan, Yuan-Li Li, Hui-Zhan Zhang, Hezhen Wu, Chang-Long Li, and Yanwen Liu
- Subjects
Pinellia ,Apoptosis ,DNA Fragmentation ,HeLa ,Glucosides ,Cell Line, Tumor ,Drug Discovery ,Humans ,MTT assay ,Glycosides ,Kaempferols ,Fragmentation (cell biology) ,Clonogenic assay ,Cell Proliferation ,Electrophoresis, Agar Gel ,Flavonoids ,Pharmacology ,Dose-Response Relationship, Drug ,biology ,Plant Extracts ,Cell Cycle ,G1 Phase ,Smilax china ,Flow Cytometry ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Biochemistry ,Agarose gel electrophoresis ,Smilax ,DNA fragmentation ,Drug Screening Assays, Antitumor ,HeLa Cells - Abstract
The anticancer activity of eight crude extracts of Smilax china L. rhizome (SCR) against HeLa cells was assessed by MTT assay and clonogenic assay, the fraction rich in flavonoids had show good activity against HeLa cells. A bioassay-guided separation on this extract lead to the detection of kaempferol-7-O-β- d -glucoside (KG), which belongs to flavonoid glycoside, displayed marked anticancer activity. We evaluated its in vitro cytotoxicity and antiproliferative effect in a panel of established cancer cell lines by MTT assay and clonogenic assay. KG induces A375 and HL60 cells apoptosis, which was demonstrated by morphological changes, DNA fragmentation and flow cytometric analysis. Fluorescent staining with Hoechst 33258 showed fragmentation and condensation of chromatin in the A375 and HL60 cells. Flow cytometric analysis shown that A375 and HL60 cells treated with KG resulted in the appearance of a hypodiploid peak (A0 region), probably due to the presence of apoptosing cells and/or apoptotic bodies with DNA content less than 2n. Quantitation of the hypodiploid cells shows a dose-dependent response to KG, and this result is in good accordance with that of the DNA fragmentation assay by agarose gel electrophoresis. Our results suggested that cell cycle arrest at G1 phase and induce apoptosis as a mechanism by which KG exerts an antiproliferative effect.
- Published
- 2007
46. Complete chloroplast genome sequence of Magnolia grandiflora and comparative analysis with related species
- Author
-
Kun Luo, Hui Yao, Huanhuan Gao, Hezhen Wu, Robert J Henry, Shilin Chen, Xiwen Li, Jingyuan Song, Zhigang Hu, Yitao Wang, Hongmei Luo, and Honglin Pan
- Subjects
Inverted repeat ,Genes, Plant ,Genome ,Magnoliaceae ,General Biochemistry, Genetics and Molecular Biology ,Evolution, Molecular ,Magnolia grandiflora ,Species Specificity ,Environmental Science(all) ,Genes, Chloroplast ,Genome, Chloroplast ,Genome size ,Phylogeny ,General Environmental Science ,Whole genome sequencing ,Genetics ,Base Composition ,Phylogenetic tree ,biology ,Agricultural and Biological Sciences(all) ,Base Sequence ,Biochemistry, Genetics and Molecular Biology(all) ,Inverted Repeat Sequences ,DNA, Chloroplast ,Chromosome Mapping ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,biology.organism_classification ,Maximum parsimony ,Magnolia ,General Agricultural and Biological Sciences ,GC-content ,Genome, Plant ,Microsatellite Repeats - Abstract
Magnolia grandiflora is an important medicinal, ornamental and horticultural plant species. The chloroplast (cp) genome of M. grandiflora was sequenced using a 454 sequencing platform and the genome structure was compared with other related species. The complete cp genome of M. grandiflora was 159623 bp in length and contained a pair of inverted repeats (IR) of 26563 bp separated by large and small single copy (LSC, SSC) regions of 87757 and 18740 bp, respectively. A total of 129 genes were successfully annotated, 18 of which included introns. The identity, number and GC content of M. grandiflora cp genes were similar to those of other Magnoliaceae species genomes. Analysis revealed 218 simple sequence repeat (SSR) loci, most composed of A or T, contributing to a bias in base composition. The types and abundances of repeat units in Magnoliaceae species were relatively conserved and these loci will be useful for developing M. grandiflora cp genome vectors. In addition, results indicated that the cp genome size in Magnoliaceae species and the position of the IR border were closely related to the length of the ycf1 gene. Phylogenetic analyses based on 66 shared genes from 30 species using maximum parsimony (MP) and maximum likelihood (ML) methods provided strong support for the phylogenetic position of Magnolia. The availability of the complete cp genome sequence of M. grandiflora provides valuable information for breeding of desirable varieties, cp genetic engineering, developing useful molecular markers and phylogenetic analyses in Magnoliaceae.
- Published
- 2012
47. [Advances of studies on new technology and method for identifying traditional Chinese medicinal materials]
- Author
-
Shilin Chen, Lin-Fang Huang, Hezhen Wu, Zhuyun Yan, Xiaohui Pang, Suqin Sun, Bao-Lin Guo, Guijun Zhang, Guangming Luo, and Jianbo Chen
- Subjects
Identification technology ,Traditional medicine ,Emerging technologies ,Biology ,Polymerase Chain Reaction ,Complementary and alternative medicine ,DNA Barcoding, Taxonomic ,Pharmacology (medical) ,Biochemical engineering ,General Pharmacology, Toxicology and Pharmaceutics ,Medicine, Chinese Traditional ,Molecular identification ,Drugs, Chinese Herbal ,Oligonucleotide Array Sequence Analysis - Abstract
In this review, the authors summarized the new technologies and methods for identifying traditional Chinese medicinal materials, including molecular identification, chemical identification, morphological identification, microscopic identification and identification based on biological effects. The authors introduced the principle, characteristics, application and prospect on each new technology or method and compared their advantages and disadvantages. In general, new methods make the result more objective and accurate. DNA barcoding technique and spectroscopy identification have their owner obvious strongpoint in universality and digitalization. In the near future, the two techniques are promising to be the main trend for identifying traditional Chinese medicinal materials. The identification techniques based on microscopy, liquid chromatography, PCR, biological effects and DNA chip will be indispensable supplements. However, the bionic identification technology is just placed in the developing stage at present.
- Published
- 2012
48. Interaction of calcineurin with its activator, chlorogenic acid revealed by spectroscopic methods
- Author
-
Jianquan Zheng, Min Jiang, Qun Wei, Yanxia Yin, Meng-Xia Xie, and Hezhen Wu
- Subjects
chemistry.chemical_classification ,Stereochemistry ,Calcineurin ,education ,General Medicine ,Biochemistry ,Binding constant ,Caprifoliaceae ,Protein Structure, Secondary ,Recombinant Proteins ,chemistry.chemical_compound ,Protein structure ,Enzyme ,Spectrometry, Fluorescence ,chemistry ,Chlorogenic acid ,parasitic diseases ,Spectroscopy, Fourier Transform Infrared ,Binding site ,Fourier transform infrared spectroscopy ,Chlorogenic Acid ,Protein secondary structure - Abstract
Chlorogenic acid (CHA) has been proved to be an activator of calcineurin (CN) in our previous research. In this study, the activation of single chain calcineurin (BA) by CHA, their interaction and concomitant changes in protein conformation were studied using fluorescence and Fourier transform infrared spectroscopy. Evidence is present that binding of CHA to CN is responsible for the stimulation of enzyme and results in structural changes. Aromatic residues reorient into new environments upon binding of CHA, the binding constant for the reaction was (2.76 ± 0.64) × 10 4 M −1 by one binding site, which indicated that CHA bound to BA statically and the change of secondary structure was mainly due to reduced α-helical content and increased β-turns. The results obtained in this study should be useful for understanding the molecular mechanisms underlying the interactions between CN and its activators.
- Published
- 2008
49. Inhibition of tumor cell proliferation by Coleon C
- Author
-
Xiu Xing, Changlong Li, Qing Li, Hezhen Wu, Yongping Huang, Yanwen Liu, Yanfang Yang, Xiaoming Wang, and Jianwen Liu
- Subjects
Programmed cell death ,Pathology ,medicine.medical_specialty ,HL60 ,Apoptosis ,Biology ,HeLa ,Lethal Dose 50 ,chemistry.chemical_compound ,Mice ,Cell Line, Tumor ,Coleus ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Fragmentation (cell biology) ,Cell Proliferation ,Pharmacology ,Dose-Response Relationship, Drug ,Cell growth ,Plant Extracts ,Lewis lung carcinoma ,biology.organism_classification ,Flow Cytometry ,Molecular biology ,Antineoplastic Agents, Phytogenic ,Infectious Diseases ,Oncology ,chemistry ,Abietanes ,DNA fragmentation - Abstract
Coleon C (6,11,12,14,16-pentahydroxyabieta-5,8,11,13-tetraen-7-one), extracted from Coleus forskohlii Briq., was investigated for its anti-tumor activity on eight human tumor cell lines (95-D, A375, HeLa, A431, MKN45, BEL7402, LoVo and HL60) and two normal ones (293, L02) by MTT and colony-forming assay in vitro. The results indicated that A375 was the most sensitive of all the cell lines. Hoechst 33258 staining showed fragmentation and condensation of chromatin. DNA ladder assay indicated the fragments of DNA because of apoptosis. Flow cytometric analysis demonstrated hypodiploid cells existed in A375 after Coleon C treatment. In the acute toxicity studies of C57BL/6 mice, LD(50 )of Coleon C was 1496+/-150 mg/kg. In the model of Lewis lung carcinoma, the average tumor weight in groups injected with 80 mg/kg Coleon C decreased by 48.9+/-14.3% compared with that of the control. These results indicate that Coleon C could effectively inhibit tumor cell proliferation and growth by inducing apoptosis with low toxicity. To our knowledge, this is the first report on the anti-tumor activity of Coleon C both in vitro and in vivo.
- Published
- 2008
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