1. Metabolomics in serum of patients with non-advanced age-related macular degeneration reveals aberrations in the glutamine pathway
- Author
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Kersten, Eveline, Dammeier, Sascha, Ajana, Soufiane, Groenewoud, Joannes M.M., Codrea, Marius, Klose, Franziska, Lechanteur, Yara T., Fauser, Sascha, Ueffing, Marius, Delcourt, Cécile, Hoyng, Carel B., de Jong, Eiko, Den Hollander, Anneke I., Arango-Gonzalez, Blanca, Arndt, Verena, Bhatia, Vaibhav, Bhatta-Charya, Shomi S., Biarnés, Marc, Borrell, Anna, Bühren, Sebastian, Calado, Sofia M., Colijn, Johanna M., Cougnard-Grégoire, Audrey, De la Cerda, Berta, Del-Court, Cécile, Diaz-Corrales, F. J., Diether, Sigrid, Emri, Eszter, Ender-Mann, Tanja, Ferraro, Lucia, Garcia, Míriam, Heesterbeek, Thomas J., Honisch, Sabina, Iacone, Roberto, Kilger, Ellen, Klaver, Caroline C.W., Langen, Hanno, Lengyel, Imre, Luthert, Phil, Maugeais, Cyrille, Meester-Smoor, Magda, Merle, Benedicte, Monés, Jordi, Nogoceke, Everson, Peto, Tunde, Pool, Fran, Rodríguez, Eduardo, Bartz-Schmidt, Karl Ulrich, Verzijden, Timo, Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Ophthalmology, and Epidemiology
- Subjects
0301 basic medicine ,genetic structures ,Metabolite ,Glutamine ,Stress-related disorders Donders Center for Medical Neuroscience [Radboudumc 13] ,Disease ,Bioinformatics ,Biochemistry ,Sensory disorders Donders Center for Medical Neuroscience [Radboudumc 12] ,chemistry.chemical_compound ,Macular Degeneration ,0302 clinical medicine ,Medicine and Health Sciences ,Metabolites ,Geriatric Ophthalmology ,Amino Acids ,Multidisciplinary ,Agricultural and Biological Sciences(all) ,Organic Compounds ,Retinal Degeneration ,Acidic Amino Acids ,Discriminant Analysis ,Neurochemistry ,Neurotransmitters ,Middle Aged ,3. Good health ,Chemistry ,Physical Sciences ,Biomarker (medicine) ,Medicine ,Retinal Disorders ,Female ,Metabolic Pathways ,Glutamate ,Metabolic Networks and Pathways ,Research Article ,Science ,03 medical and health sciences ,Metabolomics ,Age related ,medicine ,Humans ,Least-Squares Analysis ,General ,Nutrition ,Aged ,Glutaminolysis ,business.industry ,Biochemistry, Genetics and Molecular Biology(all) ,Organic Chemistry ,Chemical Compounds ,Biology and Life Sciences ,Proteins ,Macular degeneration ,medicine.disease ,eye diseases ,Diet ,Ophthalmology ,030104 developmental biology ,Metabolism ,chemistry ,Geriatrics ,Macular Disorders ,030221 ophthalmology & optometry ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,sense organs ,business ,Biomarkers ,Neuroscience - Abstract
Contains fulltext : 205500.pdf (Publisher’s version ) (Open Access) Age-related macular degeneration (AMD) is a common, progressive multifactorial vision-threatening disease and many genetic and environmental risk factors have been identified. The risk of AMD is influenced by lifestyle and diet, which may be reflected by an altered metabolic profile. Therefore, measurements of metabolites could identify biomarkers for AMD, and could aid in identifying high-risk individuals. Hypothesis-free technologies such as metabolomics have a great potential to uncover biomarkers or pathways that contribute to disease pathophysiology. To date, only a limited number of metabolomic studies have been performed in AMD. Here, we aim to contribute to the discovery of novel biomarkers and metabolic pathways for AMD using a targeted metabolomics approach of 188 metabolites. This study focuses on non-advanced AMD, since there is a need for biomarkers for the early stages of disease before severe visual loss has occurred. Targeted metabolomics was performed in 72 patients with early or intermediate AMD and 72 control individuals, and metabolites predictive for AMD were identified by a sparse partial least squares discriminant analysis. In our cohort, we identified four metabolite variables that were most predictive for early and intermediate stages of AMD. Increased glutamine and phosphatidylcholine diacyl C28:1 levels were detected in non-advanced AMD cases compared to controls, while the rate of glutaminolysis and the glutamine to glutamate ratio were reduced in non-advanced AMD. The association of glutamine with non-advanced AMD corroborates a recent report demonstrating an elevated glutamine level in early AMD using a different metabolomics technique. In conclusion, this study indicates that metabolomics is a suitable method for the discovery of biomarker candidates for AMD. In the future, larger metabolomics studies could add to the discovery of novel biomarkers in yet unknown AMD pathways and expand our insights in AMD pathophysiology.
- Published
- 2019