1. Protein C in human plasma determined by homogeneous enzyme immunoassay with use of a centrifugal analyzer
- Author
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N Kimura, T Yoshimura, Motoi S, Kita M, Etsuji Suehisa, Kiyoshi Miyai, Nishimura K, C Hayashi, M Shimamoto, and Hoshino N
- Subjects
Adult ,Clinical Biochemistry ,Enzyme-Linked Immunosorbent Assay ,Horseradish peroxidase ,Immunoenzyme Techniques ,Reference Values ,Blood plasma ,medicine ,Humans ,Horseradish Peroxidase ,Chromatography ,biology ,medicine.diagnostic_test ,Chemistry ,Liver Diseases ,Biochemistry (medical) ,Thrombosis ,Blood Proteins ,Hydrogen Peroxide ,Disseminated Intravascular Coagulation ,Standard curve ,Immunoassay ,biology.protein ,Warfarin ,Antibody ,Protein C ,medicine.drug ,Conjugate ,Peroxidase - Abstract
We describe the simple and rapid enzyme immunoassay of protein C in human plasma with use of a Cobas Fara centrifugal analyzer. The antibody, labeled with horseradish peroxidase, is reacted with antigen (protein C) for 15 min. The peroxidase activity of the resulting antigen-antibody conjugate is measured at 500 nm for 5 min in the presence of excess H2O2, phenol, and 4-aminoantipyrine, as compared with that of free conjugates. Results are calculated from a stored standard curve and expressed as a percentage of the value determined for a pooled specimen of normal adult plasma. The standard curve is linear from 0% to 200%. The CV is generally less than 4% for different concentrations of protein C. In liver cirrhosis, hepatocellular carcinoma, therapy with warfarin, thrombosis, and disseminated intravascular coagulation, protein C concentrations are about 40-70% of normal. Results obtained with the present homogeneous enzyme immunoassay correlated well with those by enzyme-labeled immunosorbent assay (r = 0.97).
- Published
- 1988