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Surface coal mining causes vegetation disturbance while providing an energy source. Thus, much attention is given to monitoring the vegetation of surface coal mining regions. Multitemporal satellite imagery, such as Landsat time-series imagery, is an operational environment monitoring service widely used to access vegetation traits and ensure vegetation surveillance across large areas. However, most of the previous studies have been conducted with change detection models or threshold-based methods that require multiple parameter settings or sample training. In this paper, we tried to analyze the change traits of vegetation in surface coal mining regions using shape-based clustering based on Normalized Difference Vegetation Index (NDVI) time series without multiple parameter settings and sample training. The shape-based clustering used in this paper applied shape-based distance (SBD) to obtain the distance between time series and used Dynamic Time Warping Barycenter Averaging (DBA) to generate cluster centroids. We applied the method to a stack of 19 NDVI images from 2000 to 2018 for a surface coal mining region located in North China. The results showed that the shape-based clustering used in this paper was appropriate for monitoring vegetation change in the region and achieved 79.0% overall accuracy in detecting disturbance-recovery trajectory types.

The phase summation effect in sum-frequency mixing process is utilized to avoid a nonlinearity obstacle in the power scaling of single-frequency visible or ultraviolet lasers. Two single-frequency fundamental lasers are spectrally broadened by phase modulation to suppress stimulated Brillouin scattering in fiber amplifier and achieve higher power. After sum-frequency mixing in a nonlinear optical crystal, the upconverted laser returns to single frequency due to phase summation, when the phase modulations on two fundamental lasers have a similar amplitude but opposite sign. The method was experimentally proved in a Raman fiber amplifier-based laser system, which generated a power-scalable sideband-free single-frequency 590 nm laser. The proposal manifests the importance of phase operation in wave-mixing processes for precision laser technology.

Despite significant progress on the design and synthesis of covalent organic frameworks (COFs), precise control over microstructures of such materials remains challenging. Herein, two chiral COFs with well-defined one-handed double-helical nanofibrous morphologies were constructed via an unprecedented template-free method, capitalizing on the diastereoselective formation of aminal linkages. Detailed time-dependent experiments reveal the spontaneous transformation of initial rod-like aggregates into the double-helical microstructures. We further demonstrated that the helical chirality and circular dichroism signal can be facilely inversed by simply adjusting the amount of acetic acid during synthesis. Moreover, by transferring chirality to achiral fluorescent molecular adsorbents, the helical COF nanostructures can effectively induce circularly polarized luminescence with the highest luminescent asymmetric factor (glum) up to ∼0.01.

Induction chemotherapy based on high-dose methotrexate is considered as the standard approach for newly diagnosed primary central nervous system lymphomas (PCNSLs). However, the best combination chemotherapeutic regimen remains unclear. This study aimed to determine the efficacy and toxicities of rituximab with methotrexate (R-M regimen). Consecutive 37 Chinese patients receiving R-M regimen as induction chemotherapy were retrospectively identified from January 2015 to June 2020 from our center in eastern China. Fourteen patients receiving rituximab plus methotrexate with cytarabine (R-MA regimen) at the same period were identified as the positive control group. The response rates, survival, toxicities, length of hospital stay (LOS), and cost were compared. Compared with the R-MA regimen, the R-M regimen showed comparable response rate and survival outcomes, but had fewer grade 3–4 hematological toxicities, shorter LOS, lower mean total hospitalization cost and lower mean total antibiotic cost. Complete remission at the end of induction chemotherapy and ECOG > 3 were independent prognostic factors for overall survival. In conclusion, R-M regimen is an effective and cost-effective combination treatment for PCNSLs, which warrants further evaluation in randomized trials.

Chimeric antigen receptor (CAR)-T cell therapy is a novel cell therapeutic approach that is increasingly being used to treat patients with relapsed refractory B-cell lymphoma. Despite the efficacy of CAR T cell therapy, it has various adverse effects that can affect any organ in the body. The application of immune checkpoint inhibitors such as programmed death 1 (PD-1), programmed death ligand 1 (PDL-1), and cytotoxic T-lymphocyte antigen 4 (CTLA-4) antibodies has previously been reported to be associated with immune-related adverse events such as thyroid dysfunction and thyroiditis. Reports of immune-related adverse reactions after CAR T therapy are currently extremely rare, with only one case of a cytokine storm (CRS) combined with severe arthritis in a patient with ALL after treatment. Here, we describe two cases of Hashimoto’s thyroiditis secondary to CAR T therapy. Two patients with relapsed refractory diffuse large B-cell lymphoma developed elevated peroxidase and globulin antibodies secondary to CAR-T cell therapy and developed Hashimoto’s thyroiditis. Complete remission was achieved in two patients at 1 and 3 months after CAR-T cell therapy. The inflammation of the thyroid tissue may be directly or indirectly related to CAR T cell therapy, and the mechanisms needs to be further investigated.

Nonlinear optical gain modulation (NOGM) in a Raman fiber amplifier is numerically simulated with the generalized nonlinear Schrödinger equation. In the NOGM setup, a single frequency continuous wave seed laser is gain modulated into femtosecond pulses by an ultrafast pump, which can induce strong stimulated Raman scattering in a piece of single mode optical fiber. Different parameters regarding seed, pump and nonlinear gain medium (Raman fiber) are investigated in detail to find the best condition for higher Raman conversion efficiency. Simulated results reveal that the walk-off between pump and Raman pulses due to dispersion is one of the most important factors affecting the NOGM pulse’s performance. Only when the speed of walk-off matches with the one of Raman conversion process can the conversion efficiency be optimized. This work offers a guild-line for the design of a fiber-based NOGM laser, which is able to produce wavelength-agile, femtosecond laser pulses with µJ-level pulse energy under more than 85% efficiency.

In order to solve the problem that the scheduling scheme cannot be updated in real time due to the dynamic change of node demand in material emergency dispatching, this article proposes a dynamic attention model based on improved gated recurrent unit. The dynamic codec framework is used to track the change of node demand to update the node information. The improved gated recurrent unit is embedded between codecs to improve the representation ability of the model. By weighted combination of the node information of the previous time, the current time and the initial time, a more representative node embedding is obtained. The results show that compared with the elitism-based immigrants ant colony optimization algorithm, the solution quality of the proposed model was improved by 27.89, 27.94, 28.09 and 28.12% when the problem scale is 10, 20, 50 and 100, respectively, which can effectively deal with the instability caused by the change of node demand, so as to minimize the cost of material distribution.

Background Leber’s congenital amaurosis (LCA) is a severe hereditary retinopathy disease that is characterized by early and severe reduction of vision, nystagmus, and sluggish or absent pupillary responses. To date, the pathogenesis of LCA remains unclear, and the majority of cases are caused by autosomal recessive inheritance. In this study, we explored the variant in the Crumbs homologue 1 (CRB1) gene in a Chinese family with LCA. Methods We conducted comprehensive ocular examinations and collected 5 ml of blood samples from members of a Chinese family with LCA. A pathogenic variant was identified by capturing (the panel in NGS) and Sanger sequencing validation. Results A nonsense variant (c.1499C>G) in the 6th exon of CRB1 gene in a Chinese family with LCA was identified, which predicted a change in the protein p. S500*, may lead to loss of gene function. We summarized the 76 variants reported thus far in CRB1 that caused LCA8. Conclusions This study reported a novel variant c.1499C>G (p. S500*) of the CRB1 gene occurred in a Chinese family with LCA, thus expanding the spectrum of CRB1 variants causing LCA.

Inter-annual Normalized Difference Vegetation Index (NDVI) time series has been applied to change detection in many fields. Research on change detection in inter-annual NDVI time series always requ...

Coal mining provides energy to support the development of society. However, mining affects the environment. Thus, substantial attention has been placed on environmental change in mining areas. Research assessing the environmental change in arid and semiarid mining areas has mainly been conducted by analyzing the variation in a single factor. However, the impact of coal mining on the environment is manifold, and there is no comprehensive assessment method. In this paper, we used a remote sensing index referred to as the comprehensive evaluation index (CEI) to comprehensively assess the environmental changes in arid and semiarid mining areas. The CEI in this study was calculated by integrating the changes in vegetation coverage (VC), land surface temperature (LST), and soil moisture (SM) using long time-series Landsat images. The Shendong mining area located in Northwest China was the study area. The results indicate that the environment of the Shendong mining area improved overall from 2000 to 2018. Environmentally degraded areas accounted for approximately 7.59% of the mining area and were concentrated in areas damaged by coal mining.

Restricted by the narrow gain bandwidth of Yb3+ near 980 nm, it is challenging to generate dispersion-managed (DM) solitons at this wavelength. In this work, we demonstrate the generation of DM solitons at 978 nm in a polarization-maintaining (PM) figure-of-9 fiber laser. Highly coherent pulses with 14.4 nm spectral bandwidth and 175 fs pulse duration are experimentally obtained. To the best of our knowledge, this is the shortest ∼980 nm pulse ever reported in an Yb-doped mode-locked fiber laser. Numerical simulations are performed to reveal the DM solitons’ temporal and spectral evolution inside the figure-of-9 cavity under the condition of a narrow gain bandwidth. This robust and cost-effective 978 nm femtosecond laser is a promising light source for applications such as underwater communication and biophotonics.

Nonlinear optical gain modulation (NOGM) is a method to generate high performance ultrafast pulses with wavelength versatility. Here we demonstrate coherent femtosecond Raman pulse generation through cascaded NOGM process experimentally. Two single-frequency seed lasers (1121 and 1178 nm) are gain-modulated by 117 nJ 1064 nm picosecond pulses in a Raman fiber amplifier. Second-order (1178 nm) Stokes pulses are generated, which have a pulse energy of 76 nJ (corresponding to an optical conversion efficiency of 65%) with a pulse duration of 621 fs (after compression). Dynamic evolution of both pump and cascaded Stokes pulses within the Raman amplifier are investigated by numerical simulations. The influences of pump pulse duration and energy are studied in detail numerically. Moreover, the simulations reveal that NOGM pulses with higher energy and shorter pulse duration could be obtained by limiting the impact of walk-off effect between pump and Raman pulses. This approach can offer a high energy and wavelength-agile ultrafast source for various applications such as optical metrology and biomedical imagining.

Background:Leber’s congenital amaurosis (LCA) is a severe hereditary retinopathy disease that is characterized by early and severe reduction of vision,nystagmus,sluggish or absent pupillary responses. To date, the pathogenesis of LCA remains unclear,and the majority cases are caused by autosomal recessive inheritance. In this study, we explored the mutation in the Crumbs homologue 1(CRB1) gene in a Chinese family with LCA. Methods:We conducted comprehensive ocular examinations and collected 5 ml of blood samples from members of a Chinese family with LCA. The pathogenic gene was identified by capturing and sequencing the related genes of ocular diseases.Results: We found a nonsense mutation(c.1499C>G) in the 6th exon of CRB1 in a Chinese family with LCA,which predicted a change of the protein p.S500X,may lead to loss of gene function.Conclusions: This study reported a novel mutation(c.1499C>G,p.S500X) of the CRB1 gene occurred in a Chinese family with LCA, thus expanding the spectrum of CRB1 mutations causing LCA. And we summarize the 76 mutations reported so far in CRB1 that caused LCA8.

Nonlinear optical gain modulation (NOGM) is a simple and effective approach to generate highly coherent ultrafast pulses with a flexible wavelength. In this work, we demonstrate 34 nJ, 170 fs pulse generation at 1319 nm through a piece of phosphorus-doped fiber by two-stage cascaded NOGM with a 1064 nm pulsed pump. Beyond the experiment, numerical results show that 668 nJ, 391 fs pulses at 1.3 µm can be produced with up to 67% conversion efficiency by increasing the pump pulse energy and optimizing the pump pulse duration. This would offer an efficient method to obtain high-energy sub-picosecond laser sources for applications such as multiphoton microscopy.

In this work, we present a monolithic single-frequency, single-mode and polarization maintaining Yb-doped fiber (YDF) amplifier delivering up to 6.9 W at 972 nm with a high efficiency of 53.6%. Core pumping at 915 nm and elevated temperature of 300 °C were applied to suppress the unwanted 977 nm and 1030 nm ASE in YDF, so as to improve the 972 nm laser efficiency. In addition, the amplifier was further used to generate a single-frequency 486 nm blue laser with 590 mW of output power by single-pass frequency doubling.

We report an investigation into secondary mode suppression in single longitudinal mode (SLM) 1240 nm diamond Raman lasers. For a three-mirror V-shape standing-wave cavity incorporating an intra-cavity LBO crystal to suppress secondary modes, we achieved stable SLM output with a maximum output power of 11.7 W and a slope efficiency 34.9%. We quantify the level of χ(2) coupling necessary to suppress secondary modes including those generated by stimulated Brillouin scattering (SBS). It is found that SBS-generated modes often coincide with higher-order spatial modes in the beam profile and can be suppressed using an intracavity aperture. Using numerical calculations, it is shown that the probability for such higher-order spatial modes is higher for an apertureless V-cavity than in two-mirror cavities due its contrasting longitudinal mode-structure.

Induction chemotherapy based on high-dose methotrexate is considered as the standard approach for newly diagnosed primary central nervous system lymphomas (PCNSLs). However, the best combination chemotherapeutic regimen remains unclear. This study aimed to determine the efficacy and toxicities of rituximab with methotrexate (R-M regimen). Consecutive 37 Chinese patients receiving R-M regimen as induction chemotherapy were retrospectively identified from January 2015 to June 2020 from our center in eastern China. Fourteen patients receiving rituximab plus methotrexate with cytarabine (R-MA regimen) at the same period were identified as the positive control group. The response rates, survival, toxicities, length of hospital stay (LOS), and cost were compared. Compared with the R-MA regimen, the R-M regimen showed comparable response rate and survival outcomes, but had fewer grade 3-4 hematological toxicities, shorter LOS, lower mean total hospitalization cost and lower mean total antibiotic cost. Overall response after two cycles of chemotherapy, complete remission at the end of induction chemotherapy and ECOG>3 were independent prognostic factors for overall survival. In conclusion, R-M regimen is an effective and well-tolerated combination treatment for PCNSLs, which warrants further evaluation in randomized trials.

High-power continuous-wave single-frequency Er-doped fiber amplifiers at 1560 nm by in-band and core pumping of a 1480 nm Raman fiber laser are investigated in detail. Both co- and counter-pumping configurations are studied experimentally. Up to 59.1 W output and 90% efficiency were obtained in the fundamental mode and linear polarization in the co-pumped case, while less power and efficiency were achieved in the counter-pumped setup for additional loss. The amplifier performs indistinguishably in terms of laser linewidth and relative intensity noise in the frequency range up to 10 MHz for both configurations. However, the spectral pedestal is raised in co-pumping, caused by cross-phase modulation between the pump and signal laser, which is observed and analyzed for the first time. Nevertheless, the spectral pedestal is 34.9 dB below the peak, which has a negligible effect for most applications.

Objective(s): Previously we reported functional leukocyte immunoglobulin-like receptor A3 (LILRA3) leads to susceptibility and sub-phenotypes of several autoimmune diseases. LILRA3 levels in blood serum and CD14+ monocytes enhanced in systemic lupus erythematosus and resulted in disease severity. However, the mechanism of LILRA3 in the pathogenesis of autoimmunity remains elusive. This study aims to explore the potential impact of LILRA3 on the differentiation, maturation, and function of monocyte-derived DCs (MoDCs). Materials and Methods: The human monocytic cell line (THP-1) was cultured to derive MoDCs in vitro. We performed plasmid transfection to examine the impact of LILRA3 on monocyte differentiation. Surface markers on MoDCs were measured using FACS. To assess the function of mature MoDCs, IL-12p70, IFN-γ and IL-4 levels were detected after the mixed leucocyte response by enzyme-linked immunosorbent assay. Western blot assay was employed in this study to determine the signaling pathways in MoDCs activation. Results: LILRA3 promotes MoDCs maturation, our results showed significant up-regulation of CD40, CD80, CD86, CD209, and HLA-DR and increased production of pro-inflammatory cytokine IL-12. LILRA3-treated MoDCs exhibited a robust proliferation of allogeneic CD4+ T cells and induced naïve CD4+ T cell polarization into the Th1 phenotype. Furthermore, the preceding activation of MoDCs maturation and LILRA3 function might be attributed to p38 MAPK and STAT1 signaling pathway’s aberrant activation. Conclusion: This is the first study to report that LILRA3 played a critical role in promoting MoDCs maturation and directing MoDCs to modulate Th1 cell differentiation, which may have a role in the pathogenesis of autoimmune diseases.

A robust 20-W continuous-wave single frequency 589 nm laser is developed to aim for sodium guide star in astronomy. The source is based on applying π-depth binary phase modulation to a single frequency seed laser along with 3 steps of strain in the gain fiber to suppress the stimulated Brillouin scattering in the high power 1178 nm amplifier and realizing the recovery of single frequency after frequency doubling in a periodically poled LiTaO3 crystal. The efficiency of frequency doubling reaches up to 41.6%. To the best of our knowledge, it is the highest power reported for continuous-wave 589 nm laser generation by single-pass frequency doubling. The approach significantly simplifies the sodium guide star laser design and improves robustness.

A chemical looping pyrolysis (CLP) system using coal tar as the fuel and Fe2O3 as the oxygen carrier (OC) was established using Aspen Plus software. The variations in reaction rate and product yiel...

Simulation and experiment were carried out as a validation methodology to study the chemical looping pyrolysis of coal tar to prepare carbon black (CB) in a fluidized bed reactor. The results demonstrate that Fe2O3 is reduced to FeO, Fe3O4, and a limited amount of Fe after the Fe-based oxygen carrier (OC) reacted with the coal tar. The gas products mainly consisted of H2 and CO, and transmission electron microscopy shows that the particle sizes of CB were in decent agreement with the simulation and the size of a single carbon sphere was approximately 40 nm. There were regular changes of the OC particle sizes during the redox process, and the OC particle size was reducing when the oxygen was releasing and was increasing when the oxygen was gaining. Therefore, the OC particle size variation contributes a suitable method to characterize the evolution of the oxygen carrying capacity. The simulations reveal the mechanisms of the chemical looping pyrolysis of coal tar with the reaction models.

This paper reports on a highly economical and accessible microfluidic pump using a piezoelectric air microblower for the controlled pumping of small-volume fluids into microfluidic chips. The present pump exerts high-accuracy control over the driving pressure to deliver fluids into and flexibly alter their flow rate inside microfluidic channels with a fast response at the millisecond scale and high pumping pressure stability. We have demonstrated the applications of this pump to shift the fluidic interface between laminar flows across a microfluidic channel, produce microdroplets with controllable sizes and chemical ingredients, and generate a discrete concentration gradient when incorporating a microfluidic concentration generator. Owing to its easy assembly and low cost, the microfluidic pump can provide a highly accessible and inexpensive solution to pumping and manipulating liquids in microfluidic devices for many chemical and biological studies, and will be particularly suitable for applications requiring full system integration and flow rate control automation within a limited space and financial budget.

A high power continuous-wave single-frequency green fiber laser by second-harmonic generation of a Yb-doped fiber amplifier (YDFA) is developed. A linearly polarized single-mode fiber amplifier produces a 60 W infrared laser at 1064 nm with a 103 W incident diode pump laser at 976 nm, corresponding to an optical conversion efficiency of 58%. An external bow-tie enhancement cavity incorporating a noncritically phase-matched lithium triborate crystal is employed for second-harmonic generation. A 33.2 W laser at 532 nm is obtained with a 45 W incident 1064 nm fundamental laser, corresponding to a conversion efficiency of 74%.

A 11.5 W 1152 nm Raman fiber laser with 6 nm spectral width was demonstrated based on the resonator constructed with one fiber loop mirror and one fiber Bragg grating. By mans of experimental measurement and theoretical calculation, the reflectivity of the fiber loop mirror was confirmed as 0.93. The Yb 3+ -doped 1090 nm fiber length was about 5 m. When the maximum pumping power of 976 nm laser was 54.8 W, 32.2 W 1090 nm laser was obtained and the optical to optical conversion efficiency from 1090 nm to 1152 nm light was 48%. Finally, the 1152 nm Raman fiber laser was used for pumping Ho 3+ :LLF crystal, and the 1194 nm fluorescence emission peak was detected for the first time.

A high power, single frequency, quasi-continuous-wave 1336 nm laser is achieved by Raman amplification of an external cavity diode laser in a variably strained polarization maintaining silica fiber. The pump laser is a 1256 nm Ytterbium-Raman integrated fiber amplifier with a maximum output peak power of 235 W. The 1336 nm amplifier produces square-shaped pulses with tunable repetition rate and duration. The peak power is as high as 53 W, which remains constant during the tuning. A polarization extinction ratio of >25 dB is achieved due to the all polarization maintaining fiber configuration. The laser is locked precisely at 1336.63 nm for future application in laser cooling of 27Al+ after 8th harmonic generation.

Wind cap partial blockages and agglomeration are two of the most common gas-solid flow faults that occur under the actual operations of circulating fluidized bed boilers. Using the method of measuring pressure fluctuations, for the characterization of fluid dynamics in fluidized beds, has a great advantage, due to its flexible adaptation to any operating conditions to monitor fluidization. This paper presents research into the use of measuring and analyzing pressure fluctuations in wind caps, for the analysis of the gas-solid fluidization characteristics in a fluidized bed with wind cap partial blockages or agglomeration fault. Partial blockages in a wind cap near feeding side and partial blockages in another wind cap near recycling side as well as agglomeration of different extents were simulated in a cold circulating fluidized bed. Pressure fluctuations in the inlets of several wind caps were measured at different primary air velocities under different fault conditions. They were then analyzed with the methods of statistical average, standard deviation, wavelet analysis and homogeneous index. Based on the calculated characteristic parameters, the effects of gas-solid flow faults on the gas-solid fluidization characteristics were analyzed. Results showed that variations of characteristic parameters of pressure fluctuations were related to variations of the gas-solid flow condition, which were caused by wind cap partial blockages or agglomerations. It is shown that the proposed method is practical.

We demonstrate a 21.2 W continuous-wave single frequency 780 nm laser by efficient single-pass frequency doubling of a 1560 nm fiber amplifier in a periodically-poled magnesium-oxide-doped lithium niobate (MgO:PPLN) crystal.

A narrowband sodium lidar for measuring mesospheric temperature and wind has been established at YangBaJing, Tibet (90°E, 30°N, 4300 m a.s.l), China. The system is designed and optimized based on important upgrades using new technology. In the lidar system, single-mode 589 nm seed laser is produced by frequency doubling of 1178 nm diode laser with a periodically poled lithium niobate (PPLN) waveguide. The output power of 589 nm continuous-wave laser is up to 1.5 W with the help of a seed-injected Raman fiber amplifier. Furthermore, fast three-frequency switcher is designed with a couple of fiber magneto optical switches (FMOS) for measuring wind and temperature, simultaneously, which greatly reduces the system maintenance. These improvements greatly simplify the lidar system, thus, achieve robust operation with minimum maintenance requirements.

An up to 8th order cascaded Raman random fiber laser with high spectral purity is achieved with the pumping of a narrow linewidth amplified spontaneous emission source. The spectral purity is over 90% for all the 8 Stokes orders. The highest output power is 6.9 W at 1691.6 nm with an optical conversion efficiency of 21% from 1062.0 nm. As a comparison, with conventional FBG-based fiber oscillator as pump source, only 47% spectral purity is achieved at 8th order. The temporal stability of the pump laser is proved to play a key role, because the time fluctuation of pump laser is transferred directly to Raman outputs and results in power distribution among different Stokes orders.

The paper reports on controlled formation of microscale wrinkles and ridges on the surface of a bioelectrode via mechanical stretching to tune and optimize the electrochemical sensing performances of graphene oxide (GO) based nitrate ion sensors. The bioelectrode consists of GO nanosheets drop-coated on a gold (Au) layer with a pre-stretched elastomer substrate. Enzyme nitrate reductase is used for covalent immobilization on the wrinkled-ridged GO surface. Upon relaxation from the pre-stretch, wrinkles or ridges are formed in the GO layer. As the pre-stretch increases, the sinusoidal wrinkles transform to localized ridges on the surface of bioelectrodes. Such morphological transitions, realized by simple mechanical stretching and relaxing, allow optimizing of the electrochemical current and sensing characteristics of the nitrate sensor. The sensing performances of the bioelectrodes at different pre-stretches are investigated. In addition to an increased electroactive surface area, the predominant localized ridges with small sinusoidal wrinkles formed on the GO surface provide a favorable spatial feature, enabling efficient radial diffusion of nitrate ions from surrounding analyte solutions onto the surface of the textured bioelectrode. At the pre-stretch of 8%, the nitrate sensor using the wrinkled-ridged bioelectrode exhibits a considerably high sensitivity of 0.224 μA L mol−1 cm−2 in response to nitrate ions, which is five times higher than that provided by the planar counterpart. Also, the textured bioelectrode shows high selectivity even in the presence of other inferring ions. The present nitrate sensor has potential applications in nitrate detection in sustainable agriculture, environmental monitoring, food analysis, and pharmaceutical industries.

With a high power Yb doped fiber laser as pump source, 10th cascaded Raman scattering to 1.95 μm in a half-open random fiber laser is demonstrated with an output power of 110 W.

Bulk NPC (nanoporous copper) were fabricated by dealloying x Al(100− x )Cu ( x = 60, 70 at.%) alloy slices in NaOH solutions (10, 20 and 30 wt.%) under free corrosion conditions. The results show that Al–Cu alloy slices can be completely dealloyed. The synergetic dealloying of Al 2 Cu and AlCu results in the formation of uniform NPC with small-sized channels. The dealloying of α-Al and Al 2 Cu plays an important role in the formation of NPC with two kinds of structures of both small-sized uniform ligaments–channels and hierarchical skeleton-like (large-sized channels with highly porous walls) structure, and these two structures form layer-cake structure.

This paper reports a microfluidic nitrate sensor using a graphene foam (GF) based electrode modified by titanium dioxide nanofibers (nTiO 2 ) and nitrate reductase enzyme molecules. The porous structure of GF allows for an easy access of nTiO 2 and enzyme molecules and attach onto the interior surfaces of GF scaffolds. This provides enhanced surface area and loading capability of enzymes for catalytic reaction with nitrate ions in soil extracts. This sensor offers a superior sensitivity of 0.316 kΩ/μM/cm2, fast detection time (∼87s), and a high selectivity to nitrate ions even in presence of many interfering ions in the soil.

We for the first time report on in-situ, real-time monitoring of nitrate and phosphorous uptake of plants grown in a microfluidic plant chip using integrated nutrient sensors. The plant chip contains the growth medium with known ingredients and initial concentrations for growing plants. As the roots uptake the nutrients from the medium, the embedded sensors detect concentration reductions of target nutrient ions in the medium. The nutrient reduction in the medium indicates the nutrient uptake by the plant. Therefore, real-time monitoring of nutrient use efficiency can be realized on the plant chip. Using rice plants as a model system, we have demonstrated accurate and continuous detection of variations of phosphate and nitrate concentrations in growth medium over 15 days. The integration of the miniature nutrient sensors with the microfluidic plant chips will provide a powerful tool in the area of plant science to help advance our understanding of plant responses to nutrient stress.

We report on the development of an enhanced and robust microfluidic plant chip-based growth and monitoring system for high-throughput phenotyping of Arabidopsis thaliana. The system consists of multiple vertical plant chips, a microfluidic hormone concentration gradient generator, multiple simple gravity pumps, and a robotic arm with a stereoscope for plant imaging. We validate this system by phenotyping the growth of Arabidopsis plants in medium containing different hormone concentrations, in a real-time manner. Our results show that Arabidopsis growth and hormone response in this system closely replicates growth on standard laboratory petri plates.

This paper reports a simple and effective method for generating a stable temperature gradient on a microfluidic plant chip. A thermo-electric cooling (TEC) plate is horizontally placed below an inclined microfluidic plant chip. The space between the TEC and the plant chip is filled with polydimethylsiloxane (PDMS). Due to different lengths of thermal pathways from the TEC to the plant chip, a stable temperature distribution is realized on the plant chip without using any feedback circuits. This present temperature gradient generation method is highly economical and accessible and can facilitate to study influences of environmental temperature changes on many microorganisms and small model plants. As an application demonstration, we present the germination and early-stage growth of Arabidopsis thaliana plant, an important model plant in the area of biology, under various temperature conditions.

Re-sequencing of target genes is a highly effective approach for identifying mutations in cancers. Mutations, including indels (insertions, deletions, and the combination of the two), play important roles in carcinogenesis. Combining genomic DNA capture using high-density oligonucleotide microarrays (NimbleGen, Inc.) with next-generation high-throughput sequencing, we identified approximately 1600 indels for colorectal cancers in the Chinese population. Among them, 5 indels were localized to exonic regions of genes, including the TFDP1 (transcription factor Dp-1) gene. TFDP1 is an important transcription factor that coordinates with E2F proteins, thereby promoting transcription of E2F target genes and regulating the cell cycle and differentiation. We report here the identification of a recurrent frame-shift indel mutation (named indel84) in the TFDP1 gene in colorectal cancers by next-generation sequencing. We found in a validation set that TFDP1 indel84 is present in 70% of colorectal cancer (CRC) tissues. Wild-type TFDP1 encodes a protein of 410 amino acids with a potential DNA binding site at its N-terminal followed by several functional protein domains. The TFDP1 indel cDNA would generate an alternative TFDP1 protein missing the first 120 amino acids and potentially affecting the DNA binding domain. We further demonstrated that the TFDP1 indel84 mutation generated a gain-of-function phenotype by increasing cell proliferation, migration, and invasion of CRC cells. Our study identified a key molecular event for CRC that might have great diagnostic and therapeutic potentials.

Robust high-power narrow-linewidth lasers at 589 nm are required for sodium laser guide star adaptive optics in astronomy. A high-power 589 nm laser based on Raman fiber amplifier is reported here, which works in both continuous-wave and pulsed formats. In the continuous-wave case, the laser produces more than 50 W output. In the pulsed case, the same laser produces square-shaped pulses with tunable repetition rate (500 Hz to 10 kHz) and duration (1 ms to 30 μs). The peak power is as high as 84 W and remains constant during the tuning. The laser also emits an adjustable sideband at 1.71 GHz away from the main laser frequency for better sodium excitation. The versatility of the laser offers much flexibility in laser guide star application.