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In this paper, we report the synthesis of silver nanoparticles (AgNPs) using AgNO3 as a precursor salt and the aqueous extracts of Eucalyptus and Larrea tridentate as reducing agents for the metal salt and stabilizing agents of the nanoparticles obtained. A solution of 25 ml of 0.1 M AgNO3 was prepared to which 10 ml of the extract was added, the reaction was kept under stirring at 50 °C for 24 hours. The extracts obtained were analyzed by the HPLC-Ms technique, to know the compounds and families that contain the aqueous extracts and identify the functional groups responsible for the reduction of Ag+ ions. This information was of great relevance to propose a reaction mechanism for the synthesis of nanoparticles. On the other hand, by means of DRX the obtaining of the AgNPs was confirmed because the results showed a crystalline nature in the cubic structure centered on the face corresponding to the silver, in addition to the Uv-vis spectroscopy the surface plasmon resonance was observed in the characteristic 430-450 nm range of these nanoparticles, the presence of functional groups on the surface of the nanoparticles was confirmed by infrared spectroscopy, and by SEM a quasi-spherical morphology and average sizes of 20-100 nm were observed using the extract of Eucalyptus and 20-60 nm with Larrea tridentate extract.