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Background: Multivariate analysis is a chemometric tool that has been little explored to determine physiological status under heavy metal stress. Nevertheless, PCA has an unexplored potential to determine the plant physiologic status and its modification under stress factors like heavy metals. Objectives: This work aims to assess the physiological and biochemical effects and responses of wheat plants under the different exposition of As and Cd using multivariate models. Materials and Methods: Wheat plants growing in a greenhouse were exposed to 0, 10 and 50 mg kg-1 soil of As and 0, 10 and 33 50 mg kg-1 soil of Cd until growth stage 5. After 56 days, wheat leaves and roots were collected to determine dry weight, lipid peroxidation and the activity of three enzymes: catalase, ascorbate peroxidase and guaiacol peroxidase. These measures were considered as the variables of three performed multivariate models to determine physiological status. Results: Through the interpretation of score plot and loading plot in combination, it was possible to determine that both As and Cd affect chlorophyll content and antioxidant response. However, a chlorophyll decrease and a lipid peroxidation increase were observed together with an inhibition of antioxidant response more accentuated in wheat plants exposed to As than those exposed to Cd. Conclusions: Multivariate analysis allows us to determine the differences between the physiological behavior of both stressors, which turn this chemometric tools useful for the characterization of a physiological response.

Background: The production, distribution, consumption, and removal of food produces a wide range of organic by-products and, eventually, waste. This kind of waste not only places a considerable burden on the environment and food economy, it also represents an opportunity to harvest additional value, often within an unexpected context and with little connection to the original food item it originates from. Objectives: Brewer’s spent grain (BSG) is such a waste material produced in considerable quantities as a by-product of the beer industry. Although traditionally discarded or fed to animals, it is rich in a wide range of interesting biomolecules and can be converted into a wide spectrum of valuable products. Results: Traditional and more innovative applications of BSG illustrate the concept of up-cycling in the food industry. With the relevant scientific and engineering base in place, BSG can be turned into a range of valuable products such as Brewer’s vinegar, creams, high-fibre bread, grain burgers, bioplastics, and pellets. Conclusion: In the medium term, rather than composting BSG for biogas or feeding it to animals, it may be seen as a valuable raw material to inspire various small- and medium-sized local industries and bedrock of an entire industry.

Background: Microorganisms commonly employed in food industry, such as Lactobacillus plantarum and Saccharomyces cerevisiae, are also excellent natural nanotechnologists. They reduce selenite (SeO3 2- ) to form nanoparticles of red selenium (Se0 ) of exceptional quality and with interesting physical and (bio-)chemical properties. Objectives: The production of these nanoparticles has been studied in several relevant microorganisms to gain a better picture of the overall properties and quality of these particles, possible differences between producers, ease of production and, in particular, biological activity. Methods: Several common microorganisms, namely L. plantarum, S. cerevisiae and Escherichia coli have been cultured under standard conditions and 1mM concentrations of SeO3 2- have been converted to red particles of elemental selenium. These particles have been characterized extensively with respect to uniformity, size, shape, consistency and, in particular, biological activity against infectious microbes. Results: Highly uniform amorphous spherical particles of 100 nm to 200 nm in diameter could be produced by several microorganisms, including Lactobacillus. Although originating in bacteria and yeast, these particles exhibit antimicrobial activity when employed at concentrations of around 100 µM. This activity may in part be due to the inherent chemistry of selenium and /or of the protein coating of the particles. Interestingly, yeast also forms larger rod-like structures. These micro-needles with around 85 nm in diameter and up to 3 µm in length exhibit considerable antibacterial activity, possibly resulting from additional, physical interactions with cellular structures. Conclusion: Common microorganisms traditionally employed in the preparation of food produce nanoparticles of selenium which may be harvested and explored as natural antimicrobial agents or antioxidants. These particles provide a fine example of and lead for natural nanotechnology with biological activity and applications in food and food supplementation, medicine, agriculture and cosmetics.

Abstract: Various medicinal plants contain phenolic compounds, which are useful in the treatment of many disease conditions. Particularly, vitexin and its isomer, isovitexin, possess many pharmacological effects including antioxidant, anti-inflammatory, anticancer, antidiabetic, neuroprotective, and antinociceptive activities. Current research has provided evidence for the prospective use of vitexin and isovitexin in the formulation of medicinal products useful in the prevention and treatment of specific ailments. The aim of this review was therefore to examine the influence of vitexin and isovitexin on the vascular system as well as the possible mechanisms through which the flavonoids exert their effects. The review also discussed the importance of vitexin and isovitexin in cardiovascular health through the vascular endothelium. In vivo and in vitro studies suggest that vitexin and isovitexin play a cardioprotective role during ischaemic reperfusion injury and angiogenesis, while isovitexin decreases perfusion pressure and increases the bioavailability of nitric oxide. Taken together, vitexin and isovitexin are promising as therapeutic agents for the formulation of nutraceuticals for the prevention, management and treatment of cardiovascular diseases.