1. InP nanowire biosensor with tailored biofunctionalization : ultrasensitive and highly selective disease biomarker detection
- Author
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Janissen, Richard, Sahoo, Prasana Kumar, 1983, Santos, Clelton Aparecido dos, 1984, Silva, Aldeliane Maria da, 1994, Von Zuben, Antonio Augusto Godoy, 1959, Souto, Dênio Emanuel Pires, 1985, Almeida, Diogo Burigo, 1983, Oliveira, Douglas Soares de, 1988, Kubota, Lauro Tatsuo, 1964, César, Carlos Lenz, 1955, Souza, Anete Pereira de, 1962, Cotta, Mônica Alonso, 1963, and UNIVERSIDADE ESTADUAL DE CAMPINAS
- Subjects
Chagas disease ,Biosensors ,Nanowires ,Biosensores ,Transistores de efeito de campo ,Artigo original ,Fosfeto de índio ,Field effect transistors ,Indium phosphide ,Surface chemistry ,Nanofios ,Doença de Chagas ,Química de superfície - Abstract
Agradecimentos: R.J., P.K.S. and D.S.O. acknowledge scholarships from FAPESP and A.M.S. and D.B.A. from CAPES/CNPq. Access to confocal microscopy was granted by INFABIC/UNICAMP (FAPESP 08/57906-3, CNPq 573913/2008-0). We acknowledge access to electron microscopy (LME) and microfabrication (LMF) facilities at the National Nanotechnology Laboratory (LNNano, Brazil) and clean room facilities at both Semiconductor Component Center (CCSNano, UNICAMP) and Device Research Laboratory (IFGW, UNICAMP). Xf.XadA2 antibodies were provided by Dr. A. A. de Souza (IAC, Cordeirópolis, São Paulo, Brazil). The authors are greatly indebted to A. Gobbi and M.H. Piazzetta from LMF/LNNano for PDMS microfluidics processing, Dr. A. Kisner from IQ/UNICAMP and P .C. Galvão, from IQ/UNESP, for technical assistance during this work Abstract: Electrically active field-effect transistors (FET) based biosensors are of paramount importance in life science applications, as they offer direct, fast, and highly sensitive label-free detection capabilities of several biomolecules of specific interest. In this work, we report a detailed investigation on surface functionalization and covalent immobilization of biomarkers using biocompatible ethanolamine and poly(ethylene glycol) derivate coatings, as compared to the conventional approaches using silica monoliths, in order to substantially increase both the sensitivity and molecular selectivity of nanowire-based FET biosensor platforms. Quantitative fluorescence, atomic and Kelvin probe force microscopy allowed detailed investigation of the homogeneity and density of immobilized biomarkers on different biofunctionalized surfaces. Significantly enhanced binding specificity, biomarker density, and target biomolecule capture efficiency were thus achieved for DNA as well as for proteins from pathogens. This optimized functionalization methodology was applied to InP nanowires that due to their low surface recombination rates were used as new active transducers for biosensors. The developed devices provide ultrahigh label-free detection sensitivities similar to 1 fM for specific DNA sequences, measured via the net change in device electrical resistance. Similar levels of ultrasensitive detection of similar to 6 fM were achieved for a Chagas Disease protein marker (IBMP8-1). The developed InP nanowire biosensor provides thus a qualified tool for detection of the chronic infection stage of this disease, leading to improved diagnosis and control of spread. These methodological developments are expected to substantially enhance the chemical robustness, diagnostic reliability, detection sensitivity, and biomarker selectivity for current and future biosensing devices FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES Fechado
- Published
- 2017