Liu, Guoquan, Zhang, Yan, Gong, Hao, Li, Shan, Yunrong Pan, Davis, Christopher, Jing, Hai-Chun, Luguang Wu, and Godwin, Ian D.
Additional file 1: Fig. S1. Transgenic sorghum lines were grown in a PC2 glasshouse. (a) 1 week; (b) 2 weeks; (c) 3 weeks; (d) 5 weeks; (e) 7 weeks; (f) 10 weeks in the glasshouse; (g) mature T×430; (h) mature A5; (f) mature L9. Fig. S2. The Construct Used for Gene Transformation. Stem-specific Promoter: either A1 resulting in high gene expression in the mature stalk or LSG2 resulting in high gene expression in the loading sucrose section of the stalk. Vacuole leading: encoding a propeptide to guide SI gene products to vacuole, where sucrose accumulates. Non-silence target gene: sucrose isomerase without motifs gene silencing in plants. Multiple terminators: three recombined terminators complex to guarantee the proper termination of gene transcription. Fig. S3. PCR screening of sucrose isomerase gene in transgenic LSG2 lines. The agarose gel displayed PCR results of transgenic lines. M: DNA ladder; NC: negative control; Transgenic lines L1, L2, L3, L4, L5, L7, L9, L14, and L16 were positive of the sucrose isomerase gene; Transgenic lines L6, L8, L10, L11, L12, L13,L15, and L17 were negative of the sucrose isomerase gene. Fig. S4. Sugar profile of T1 L9 lines. Sugars, including isomaltulose, were measured 20-day post-anthesis in the middle section of internode 4 (counted from top). L9 is one of the top lines in T0 transgenic lines. L9-2, 9-3, 9-6, 9-7, 9-11, 9-12 are isomaltulose positive samples. Nil-L9-4, 9-8 and 9-9 are null-segregant samples in T1 generation. T×430-1, T×430-2, and T×430-3 are the non-transformed control. Fig. S5. PCR screening of sucrose isomerase gene in T1 lines. The agarose gel displayed PCR results of transgenic lines and controls. M: 1 Kb DNA ladder; Positive A5 T1 progenies: A5-2 and A5-4; Positive L2 T1 progenies: L2-1, L2-3 and L2-6; Positive L9 T1 progenies: L9-2, and L9-4; T×430-1, T×430-2 are non-transgenic control samples; PC: positive plasmid control (LSG2). Table S1. Sugar profile of controls and positive transgenic lines with isomaltulose. Table S2. Sugar profile of F1 hybrid lines of R9188 X L9.